甘露消毒丹及其挥发油对流感病毒感染小鼠黏膜免疫的影响
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摘要
目的:观察甘露消毒丹(GXM)及其挥发油对流感病毒(IV)感染的BALB/c小鼠免疫球蛋白、细胞因子的含量变化以探讨其对黏膜免疫的影响。方法:SPF级BALB/c小鼠108只按照随机数字表法分为6组,分别为:正常组(A组)、模型组(B组)、GXM灌胃组(C组)、GXM滴鼻组(D组)、GXM合用组(E组)、奥司他韦组(F组),每组18只。除A组动物外,其余5组均以流感病毒FM1-6-E2滴鼻制备感染模型。于造模后第3、7、10天采集标本,采用ELISA方法检测肺泡灌洗液(BALF)及鼻咽冲洗液(NAIF)中sIgA、血清中IgA、IL-5、IL-6含量。结果:IV感染小鼠后,NAIF和BALF中sIgA含量降低;血清中IL-5、IL-6水平升高,而IgA含量降低。与B组比较,D、E组能够升高NAIF中sIgA含量,而C组在第10天升高明显(P<0.05),F组作用不明显(P>0.05);E组能够升高血清中IgA、BALF中sIgA含量,而C组、D组分别在第7天、第10天升高明显(P<0.05),F组作用不明显(P>0.05);C、E、F组能够降低IL-5、IL-6含量(P<0.05),而D组作用不明显(P>0.05)。结论:GXM挥发油可促进sIgA的分泌且起效迅速,GXM醇提物可升高IgA、sIgA含量,降低IL-5、IL-6含量,两种给药方式合用效果更佳。
Objective:To observe the changes of immunoglobulin and cytokines in BALB/c mice infected with influenza viruses by using Ganlu Xiaodu Micropill(GXM) and its volatile oil to investigate its effect on mucosal immunity.Methods:Totally 108 SPF BALB/c mice were randomly divided into 6 groups according to random number table,respectively:normal group(Group A),model grape(Group B),filling GXM to the stomach group(Group C),dripping GXM to the nasal cavity(Group D),GXM combined group(Group E)and oseltamivir group(Group F),each group 18.In addition to the group A,we used nasalinoculation of IV FM1-6-E2 in mice to produce model.Each group's mice were infected on 3,7,10 day. And then mice were sacrificed,detecting the levels of sIgA in the miceBALF and NAIF,IgA,IL-5 and IL-6 in the mice bloodwith the method of ELISA.Results:After the infection of IV,SIgA levels decreased in NAIF and BALF;the levels of IL-5 and IL-6 in the mice blood were increased, but the level of IgA was decreased.Compared with group B,D, E group can increase the level of sIgA in NAIF,while that of the group C increased significantly on the 10 th day(P<0.05). The level of sIgA of F group was not obvious(P>0.05).E group can increase the levels of IgA, sIgA in BALF of C group and D group, respectively. On the 7 the and 10 the day,those increased significantly(P<0.05). Those of F group was not obvious(P>0.05). C, E, F group can reduce the levels of IL-5 and IL-6(P<0.05). D group'swere not obvious(P>0.05).Conclusion:GXM volatile oil can promote the secretion of sIgA and rapid onset. GXM alcohol extract can increasethe level of IgA and sIgA,reduce the levels of IL-5 and IL-6. The combination of the two model is better.
Objective:To observe the changes of immunoglobulin and cytokines in BALB/c mice infected with influenza viruses by using Ganlu Xiaodu Micropill(GXM) and its volatile oil to investigate its effect on mucosal immunity.Methods:Totally 108 SPF BALB/c mice were randomly divided into 6 groups according to random number table,respectively:normal group(Group A),model grape(Group B),filling GXM to the stomach group(Group C),dripping GXM to the nasal cavity(Group D),GXM combined group(Group E)and oseltamivir group(Group F),each group 18.In addition to the group A,we used nasalinoculation of IV FM1-6-E2 in mice to produce model.Each group's mice were infected on 3,7,10 day. And then mice were sacrificed,detecting the levels of sIgA in the miceBALF and NAIF,IgA,IL-5 and IL-6 in the mice bloodwith the method of ELISA.Results:After the infection of IV,SIgA levels decreased in NAIF and BALF;the levels of IL-5 and IL-6 in the mice blood were increased, but the level of IgA was decreased.Compared with group B,D, E group can increase the level of sIgA in NAIF,while that of the group C increased significantly on the 10 th day(P<0.05). The level of sIgA of F group was not obvious(P>0.05).E group can increase the levels of IgA, sIgA in BALF of C group and D group, respectively. On the 7 the and 10 the day,those increased significantly(P<0.05). Those of F group was not obvious(P>0.05). C, E, F group can reduce the levels of IL-5 and IL-6(P<0.05). D group'swere not obvious(P>0.05).Conclusion:GXM volatile oil can promote the secretion of sIgA and rapid onset. GXM alcohol extract can increasethe level of IgA and sIgA,reduce the levels of IL-5 and IL-6. The combination of the two model is better.
引文
[1] 郭圣敏.中药预防流感的作用与黏膜免疫相关性探讨[J]. 中国实用医药, 2015(14):291-292.
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[9] 李春梅.分泌型IgA联合常规抗感染药物治疗对小儿上呼吸道感染炎症反应、免疫应答的影响[J].海南医学院学报,2018,24(1):60-63.
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[13] 柳丽松, 柳弯, 李艳,等. 分泌型免疫球蛋白A在黏膜免疫中的作用[J]. 医学综述 ,2015,21(11):1927-1929.
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[15] 南淑玲, 徐顺富, 陈许,等.升降散对流感病毒鼠肺适应株FM1感染小鼠免疫功能的影响[J].中药药理与临床, 2016,32(5):8-13.
[16] 刘光华,王贵帮,杨慧,等.甘露消毒丹制方特点探微[J].四川中医,2017,35(11):32-35.
[17] 刘晓婷, 张沂, 顾立刚,等.流感病毒H1N1感染A549细胞诱导凋亡及黄芩苷干预作用的研究[J].中国药理学通报, 2015,31(7):936-939.
[18] 张美玲,李峰,王聪聪,等.连翘抗病毒作用研究进展[J].辽宁中医药大学学报,2016,18(10):130-132.
[19] 王安庆.茵陈的现代研究进展[J].光明中医,2014,29(10):2207-2208.
[20] 徐雯,吴艳清,丁浩然,等.广藿香的药理作用及机制研究进展[J].上海中医药杂志,2017,51(10):103-106.
[21] 毕丽叶,聂晶.滑石功用拾遗[J].中国中医基础医学杂志,2017,23(8):1152-1153,1173.
[2] 邵雨萌, 杨景青. 抗流感药物应用现状及展望[J]. 湖南中医杂志, 2013, 29(9):143-145.
[3] Li JH,Wang RQ,Guo WJ,et al. Efficacy and safety of traditional Chinese medicine for the treatment of influenza A (H1N1): A meta-analysis[J]. Journal of the Chinese Medical Association,2016,79(5):522-525.
[4] 张永欣,赵斐,王宇佳,等.新型siRNA抗流感病毒活性的研究[J].中国医药生物技术,2017,12(3):207-214.
[5] 刘光华,吴振起,刘娟.从湿毒论治雾霾环境下流感病毒感染[J].吉林中医药,2018,38(2):181-184.
[6] 毕岩,岳冬辉,高玉伟,等.甘露消毒丹对H1N1流感病毒感染小鼠细胞因子的影响[J].中华中医药杂志,2014,29(12):3950-3953.
[7] 韩云,邹俊驹,贺又舜.甘露消毒丹的来源与现代应用概况[J].湖南中医杂志,2017,33(5):181-183.
[8] 王剑,田志刚.呼吸道黏膜免疫及其相关疾病研究进展[J].中国免疫学杂志,2015,31(3):289-294.
[9] 李春梅.分泌型IgA联合常规抗感染药物治疗对小儿上呼吸道感染炎症反应、免疫应答的影响[J].海南医学院学报,2018,24(1):60-63.
[10] 岳冬辉,毕岩,宋岩,等.中医药预防流感与黏膜免疫相关性研究[J].中医药临床杂志, 2015,12(27):1655-1658.
[11] 张印,崔艺馨,杨明会.呼吸道变应性炎症相关Th细胞因子的研究进展[J].中国医药导报,2016,13(16):31-34.
[12] 赵化阳, 刘金华, 刘云才. H1N1流感病毒感染小鼠在免疫学研究中的初步应用[J]. 实验动物科学, 2016, 33(1):24-28.
[13] 柳丽松, 柳弯, 李艳,等. 分泌型免疫球蛋白A在黏膜免疫中的作用[J]. 医学综述 ,2015,21(11):1927-1929.
[14] Betakova Tatiana,Kostrabova Anna,Lachova Veronika,et al. Cytokines induced during influenza virus infection[J]. Current pharmaceutical design, 2017,23(18):2616-2622.
[15] 南淑玲, 徐顺富, 陈许,等.升降散对流感病毒鼠肺适应株FM1感染小鼠免疫功能的影响[J].中药药理与临床, 2016,32(5):8-13.
[16] 刘光华,王贵帮,杨慧,等.甘露消毒丹制方特点探微[J].四川中医,2017,35(11):32-35.
[17] 刘晓婷, 张沂, 顾立刚,等.流感病毒H1N1感染A549细胞诱导凋亡及黄芩苷干预作用的研究[J].中国药理学通报, 2015,31(7):936-939.
[18] 张美玲,李峰,王聪聪,等.连翘抗病毒作用研究进展[J].辽宁中医药大学学报,2016,18(10):130-132.
[19] 王安庆.茵陈的现代研究进展[J].光明中医,2014,29(10):2207-2208.
[20] 徐雯,吴艳清,丁浩然,等.广藿香的药理作用及机制研究进展[J].上海中医药杂志,2017,51(10):103-106.
[21] 毕丽叶,聂晶.滑石功用拾遗[J].中国中医基础医学杂志,2017,23(8):1152-1153,1173.