交联酶聚集体的制备及在漆酶固定化中的应用进展
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摘要
漆酶是一种含铜的多酚氧化酶,其催化效率高,作用底物范围广,在工业中具有广阔应用前景。交联酶聚集体(CLEAs)是一种无需载体、无需酶纯化、制备简单、成本低的新型固定化酶方法,通常具有较高的稳定性,可实现酶的重复利用。对交联酶聚体制备过程中影响酶活性的相关因素以及其改进策略进行了分析,并对交联漆酶聚集体在污染物降解等方面的应用进行了总结,旨在为进一步的研究和应用奠定基础。
Laccase is a copper-containing polyphenol oxidase with high catalytic efficiency. Due to its broad substrate range,laccase has been widely used in many industrial fields. Cross-linked enzyme aggregates(CLEAs)technology is a novel carrier-free enzyme immobilization approach that could be simply performed using crude enzyme at low cost. The CLEAs usually have high stability and can be reused in multiple cycles. In this paper,we first analyzed the factors affecting enzyme activity during the CLEAs preparation and the related improving strategies,and then summarized the application of cross-linked laccase aggregates in pollutant degradation,with the aim of laying a foundation for further research and application of immobilized laccase.
Laccase is a copper-containing polyphenol oxidase with high catalytic efficiency. Due to its broad substrate range,laccase has been widely used in many industrial fields. Cross-linked enzyme aggregates(CLEAs)technology is a novel carrier-free enzyme immobilization approach that could be simply performed using crude enzyme at low cost. The CLEAs usually have high stability and can be reused in multiple cycles. In this paper,we first analyzed the factors affecting enzyme activity during the CLEAs preparation and the related improving strategies,and then summarized the application of cross-linked laccase aggregates in pollutant degradation,with the aim of laying a foundation for further research and application of immobilized laccase.
引文
[1]Fernández-Fernández M, Sanromán Má, Moldes D. Recent developments and applications of immobilized laccase[J].Biotechnology Advances, 2013, 31(8):1808-1825.
[2]司静,李伟,崔宝凯,等.真菌漆酶性质、分子生物学及其应用研究进展[J].生物技术通报, 2011, 2:48-55.
[3]司静,崔宝凯,戴玉成.栓孔菌属漆酶高产菌株的初步筛选及其产酶条件的优化[J].微生物学通报, 2011, 38(3):405-416.
[4]BernalC,RodríguezK,MartínezR.Integratingenzyme immobilization and protein engineering:An alternative path for the development of novel and improved industrial biocatalysts[J].Biotechnology Advances, 2018, 36(5):1470-1480.
[5]Sheldon RA, Schoevaart R, Langen LMV. Cross-linked enzyme aggregates(CLEAs):A novel and versatile method for enzyme immobilization(a review)[J]. Biocatalysis, 2005, 23(3):141-147.
[6]VoběrkováS, Sol?ány V, Vr?anskáM, et al. Immobilization of ligninolytic enzymes from white-rot fungi in cross-linked aggregates[J]. Chemosphere, 2018, 202:694-707.
[7]Cui JD, Jia SR. Optimization protocols and improved strategies of cross-linked enzyme aggregates technology:Current development and future challenges[J]. Critical Reviews in Biotechnology,2015, 35(1):15-28.
[8]Talekar S, Joshi A, Joshi G, et al. Parameters in preparation and characterization of cross linked enzyme aggregates(CLEAs)[J].RSC Advances, 2013, 3(31):12485-12511.
[9]Perzon A, Dicko C,?obano?lu?, et al. Cellulase cross-linked enzyme aggregates(CLEA)activities can be modulated and enhanced by precipitant selection[J]. Journal of Chemical Technology and Biotechnology, 2017, 92(7):1645-1649.
[10]Bilal M, Asgher M, Iqbal HM, et al. Bio-based degradation of emerging endocrine-disrupting and dye-based pollutants using cross-linked enzyme aggregates[J]. Environmental Science and Pollution Research, 2017, 24(8):7035-7041.
[11]Aytar BS, Bakir U. Preparation of cross-linked tyrosinase aggregates[J]. Process Biochemistry, 2008, 43(2):125-131.
[12]Li SP, Su YT, Liu YD, et al. Preparation and characterization of cross-linked enzyme aggregates(CLEAs)of recombinant thermostable alkylsulfatase(SdsAP)from Pseudomonas sp S9[J]. Process Biochemistry, 2016, 51(12):2084-2089.
[13]Mateo C, Palomo JM, Langen, LMV, et al. A new, mild cross-linking methodology to prepare cross-linked enzyme aggregates[J].Biotechnology and Bioengineering, 2004, 86(3):273-276.
[14]Zerva A, Antonopoulou I, Enman J, et al. Cross-linked enzyme aggregates of feruloyl esterase preparations from Thermothelomyces thermophila and Talaromyces wortmannii[J]. Catalysts, 2018, 8(5):208.
[15]Yang J, Xu X, Yang X, et al. Cross-linked enzyme aggregates of Cerrena laccase:Preparation, enhanced NaCl tolerance and decolorization of Remazol Brilliant Blue Reactive[J]. Journal of the Taiwan Institute of Chemical Engineers, 2016, 65:1-7.
[16]Zheng GW, Yu HL, Li CHX, et al. Immobilization of Bacillus subtilis esterase by simple cross-linking for enzymatic resolution of DL-menthyl acetate[J]. Journal of Molecular Catalysis B:Enzymatic, 2011, 70(3-4):138-143.
[17]Dalal S, Kapoor M, Gupta MN. Preparation and characterization of combi-CLEAs catalyzing multiple non-cascade reactions[J].Journal of Molecular Catalysis B:Enzymatic, 2007, 44(3-4):128-132.
[18]Bolar S, Iyyaswami R, Belur PD. Purification of glutaminase from Zygosaccharomyces rouxii in polyethylene glycol-sodium sulphate aqueous two-phase system[J]. Separation Science and Technology, 2015, 50(9):1360-1368.
[19]Migneault I, Dartiguenave C, Bertrand MJ, et al. Glutaraldehyde:behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking[J]. Biotechniques, 2004 37(5):790-802.
[20]Shah S, Sharma A, Gupta MN. Preparation of cross-linked enzyme aggregates by using bovine serum albumin as a proteic feeder[J].Analytical Biochemistry, 2006, 351(2):207-213.
[21]Cui JD, Liu RL, Li LB. A facile technique to prepare crosslinked enzyme aggregates of bovine pancreatic lipase using bovine serum albumin as an additive[J]. Korean Journal of Chemical Engineering, 2016, 33(2):610-615.
[22]TorabizadehH,TavakoliM,SafariM.Immobilizationof thermostableα-amylase from Bacillus licheniformis by crosslinked enzyme aggregates method using calcium and sodium ions as additives[J]. Journal of Molecular Catalysis B:Enzymatic,2014, 108(108):13-20.
[23]Gupta P, Dutt K, Misra S, et al. Characterization of cross-linked immobilized lipase from thermophilic mould Thermomyces lanuginosa using glutaraldehyde[J]. Bioresource Technology,2009, 100(18):4074-4076.
[24]Wang MF, Qi W, Yu QX, et al. Cross-linking enzyme aggregates in the macropores of silica gel:a practical and efficient method for enzyme stabilization[J]. Biochemical Engineering Journal, 2010,52(2):168-174.
[25]Reshmi R, Sugunan S. Improved biochemical characteristics of cross linkedβ-glucosidase on nanoporous silica foams[J].Journal of Molecular Catalysis B:Enzymatic, 2013, 85-86:111-118.
[26]Park JM, Kim M, Park HS, et al. Immobilization of lysozyme-CLEA onto electrospun chitosan nanofiber for effective antibacterial applications[J]. Int J Biol Macromol, 2013, 54(1):37-43.
[27]Singh RK, Tiwari MK, Singh R, et al. From protein engineering to immobilization:promising strategies for the upgrade of industrial enzymes[J]. International Journal of Molecular Sciences, 2013,14(1):1232-1277.
[28]杨猛,江惠娟,宁晨曦,等.复合交联酶聚集体的制备及催化羰基不对称还原合成手性醇[J].高等学校化学报, 2018, 39(1):54-63.
[29]Periyasamy K, Santhalembi, L, Mortha G, et al. Carrier-free coimmobilization of xylanase, cellulase andβ-1, 3-glucanase as combined cross-linked enzyme aggregates(combi-CLEAs)for one-pot saccharification of sugarcane bagasse[J]. RSC Advances, 2016, 6(39):32849-32857.
[30]Sheldon RA. Characteristicfeatures and biotechnological applications of cross-linked enzyme aggregates(CLEAs)[J].Applied Microbiology and Biotechnology, 2011, 92(3):467-477.
[31]Su E, Meng Y, Ning C, et al. Magnetic combined cross-linked enzyme aggregates(Combi-CLEAs)for cofactor regeneration in the synthesis of chiral alcohol[J]. J Biotechnol, 2018, 271:1-7.
[32]Sóti V, Lenaerts S, Cornet I. Of enzyme use in cost-effective high solid simultaneous saccharification and fermentation processes[J]. J Biotechnol, 2018, 270:70-76.
[33]Singh RL, Singh PK. Singh RP. Enzymatic decolorization and degradation of azo dyes-A review[J]. International Biodeterioration&Biodegradation, 2015, 104:21-31.
[34]Kuma VV, Kumar MPP, Thiruvenkadaravi KV, et al. Preparation and characterization of porous cross linked laccase aggregates for the decolorization of triphenyl methane and reactive dyes[J].Bioresource Technology, 2012, 119(9):28-34.
[35]Vr?anskáM, VoběrkováS, Jiménez AJ, et al. Preparation and optimisation of cross-linked enzyme aggregates using native isolate white rot fungi Trametes versicolor and Fomes fomentarius for the decolourisation of synthetic dyes[J]. International Journal of Environmental Research and Public Health, 2017, 15(1):23.
[36]Kumar VV, Sivanesan S, Cabana H. Magnetic cross-linked laccase aggregates-Bioremediation tool for decolorization of distinct classes of recalcitrant dyes[J]. Science of the Total Environment, 2014,487(1):830-839.
[37]Cabana H, Jones JP, Agathos SN. Utilization of cross-linked laccase aggregates in a perfusion basket reactor for the continuous elimination of endocrine-disrupting chemicals[J]. Biotechnology and Bioengineering, 2009, 102(6):1582-1592.
[38]SinirliogluZA,SinirliogluD,AkbasF.Preparationand characterization of stable cross-linked enzyme aggregates of novel laccase enzyme from Shewanella putrefaciens and using malachite green decolorization[J]. Bioresource Technology, 2013, 146(10):807-811.
[39]Jeong J, Song W, Cooper WJ, et al. Degradation of tetracycline antibiotics:mechanisms and kinetic studies for advanced oxidation/reduction processes[J]. Chemosphere, 2010, 78(5):533-540.
[40]Yang J, Lin Y, Yang X, et al. Degradation of tetracycline byimmobilizedlaccaseandtheproposedtransformation pathway[J]. Journal of Hazardous Materials, 2017, 322:525-531.
[41]Kumar VV, Cabana H. Towards high potential magnetic biocatalysts for on-demand elimination of pharmaceuticals[J]. Bioresource Technology, 2016, 200:81-89.
[42]Jiang Y, Wang Q, He Y, et al. Co-aggregation of laccase and nature egg white:a simple method to prepare stable and recyclable biocatalyst[J]. Applied Biochemistry and Biotechnology, 2014,172(5):2496-2506.
[43]Matijo?yt?I, Arends IWCE, Vries SD, et al. Preparation and use of cross-linked enzyme aggregates(CLEAs)of laccases[J].Journal of Molecular Catalysis B:Enzymatic, 2010, 62(2):142-148.
[44]Nghiem LN, Hai FI, Kang J, et al. Enhancement of trace organic contaminant degradation by crude enzyme extract from Trametes versicolor culture:Effect of mediator type and concentration[J].Journal of the Taiwan Institute of Chemical Engineers, 2014, 45(4):1855-1862.
[45]Chauhan PS, Goradia B, Saxena A. Bacterial laccase:recent update on production, properties and industrial applications[J].3 Biotech, 2017, 7(5):323.
[2]司静,李伟,崔宝凯,等.真菌漆酶性质、分子生物学及其应用研究进展[J].生物技术通报, 2011, 2:48-55.
[3]司静,崔宝凯,戴玉成.栓孔菌属漆酶高产菌株的初步筛选及其产酶条件的优化[J].微生物学通报, 2011, 38(3):405-416.
[4]BernalC,RodríguezK,MartínezR.Integratingenzyme immobilization and protein engineering:An alternative path for the development of novel and improved industrial biocatalysts[J].Biotechnology Advances, 2018, 36(5):1470-1480.
[5]Sheldon RA, Schoevaart R, Langen LMV. Cross-linked enzyme aggregates(CLEAs):A novel and versatile method for enzyme immobilization(a review)[J]. Biocatalysis, 2005, 23(3):141-147.
[6]VoběrkováS, Sol?ány V, Vr?anskáM, et al. Immobilization of ligninolytic enzymes from white-rot fungi in cross-linked aggregates[J]. Chemosphere, 2018, 202:694-707.
[7]Cui JD, Jia SR. Optimization protocols and improved strategies of cross-linked enzyme aggregates technology:Current development and future challenges[J]. Critical Reviews in Biotechnology,2015, 35(1):15-28.
[8]Talekar S, Joshi A, Joshi G, et al. Parameters in preparation and characterization of cross linked enzyme aggregates(CLEAs)[J].RSC Advances, 2013, 3(31):12485-12511.
[9]Perzon A, Dicko C,?obano?lu?, et al. Cellulase cross-linked enzyme aggregates(CLEA)activities can be modulated and enhanced by precipitant selection[J]. Journal of Chemical Technology and Biotechnology, 2017, 92(7):1645-1649.
[10]Bilal M, Asgher M, Iqbal HM, et al. Bio-based degradation of emerging endocrine-disrupting and dye-based pollutants using cross-linked enzyme aggregates[J]. Environmental Science and Pollution Research, 2017, 24(8):7035-7041.
[11]Aytar BS, Bakir U. Preparation of cross-linked tyrosinase aggregates[J]. Process Biochemistry, 2008, 43(2):125-131.
[12]Li SP, Su YT, Liu YD, et al. Preparation and characterization of cross-linked enzyme aggregates(CLEAs)of recombinant thermostable alkylsulfatase(SdsAP)from Pseudomonas sp S9[J]. Process Biochemistry, 2016, 51(12):2084-2089.
[13]Mateo C, Palomo JM, Langen, LMV, et al. A new, mild cross-linking methodology to prepare cross-linked enzyme aggregates[J].Biotechnology and Bioengineering, 2004, 86(3):273-276.
[14]Zerva A, Antonopoulou I, Enman J, et al. Cross-linked enzyme aggregates of feruloyl esterase preparations from Thermothelomyces thermophila and Talaromyces wortmannii[J]. Catalysts, 2018, 8(5):208.
[15]Yang J, Xu X, Yang X, et al. Cross-linked enzyme aggregates of Cerrena laccase:Preparation, enhanced NaCl tolerance and decolorization of Remazol Brilliant Blue Reactive[J]. Journal of the Taiwan Institute of Chemical Engineers, 2016, 65:1-7.
[16]Zheng GW, Yu HL, Li CHX, et al. Immobilization of Bacillus subtilis esterase by simple cross-linking for enzymatic resolution of DL-menthyl acetate[J]. Journal of Molecular Catalysis B:Enzymatic, 2011, 70(3-4):138-143.
[17]Dalal S, Kapoor M, Gupta MN. Preparation and characterization of combi-CLEAs catalyzing multiple non-cascade reactions[J].Journal of Molecular Catalysis B:Enzymatic, 2007, 44(3-4):128-132.
[18]Bolar S, Iyyaswami R, Belur PD. Purification of glutaminase from Zygosaccharomyces rouxii in polyethylene glycol-sodium sulphate aqueous two-phase system[J]. Separation Science and Technology, 2015, 50(9):1360-1368.
[19]Migneault I, Dartiguenave C, Bertrand MJ, et al. Glutaraldehyde:behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking[J]. Biotechniques, 2004 37(5):790-802.
[20]Shah S, Sharma A, Gupta MN. Preparation of cross-linked enzyme aggregates by using bovine serum albumin as a proteic feeder[J].Analytical Biochemistry, 2006, 351(2):207-213.
[21]Cui JD, Liu RL, Li LB. A facile technique to prepare crosslinked enzyme aggregates of bovine pancreatic lipase using bovine serum albumin as an additive[J]. Korean Journal of Chemical Engineering, 2016, 33(2):610-615.
[22]TorabizadehH,TavakoliM,SafariM.Immobilizationof thermostableα-amylase from Bacillus licheniformis by crosslinked enzyme aggregates method using calcium and sodium ions as additives[J]. Journal of Molecular Catalysis B:Enzymatic,2014, 108(108):13-20.
[23]Gupta P, Dutt K, Misra S, et al. Characterization of cross-linked immobilized lipase from thermophilic mould Thermomyces lanuginosa using glutaraldehyde[J]. Bioresource Technology,2009, 100(18):4074-4076.
[24]Wang MF, Qi W, Yu QX, et al. Cross-linking enzyme aggregates in the macropores of silica gel:a practical and efficient method for enzyme stabilization[J]. Biochemical Engineering Journal, 2010,52(2):168-174.
[25]Reshmi R, Sugunan S. Improved biochemical characteristics of cross linkedβ-glucosidase on nanoporous silica foams[J].Journal of Molecular Catalysis B:Enzymatic, 2013, 85-86:111-118.
[26]Park JM, Kim M, Park HS, et al. Immobilization of lysozyme-CLEA onto electrospun chitosan nanofiber for effective antibacterial applications[J]. Int J Biol Macromol, 2013, 54(1):37-43.
[27]Singh RK, Tiwari MK, Singh R, et al. From protein engineering to immobilization:promising strategies for the upgrade of industrial enzymes[J]. International Journal of Molecular Sciences, 2013,14(1):1232-1277.
[28]杨猛,江惠娟,宁晨曦,等.复合交联酶聚集体的制备及催化羰基不对称还原合成手性醇[J].高等学校化学报, 2018, 39(1):54-63.
[29]Periyasamy K, Santhalembi, L, Mortha G, et al. Carrier-free coimmobilization of xylanase, cellulase andβ-1, 3-glucanase as combined cross-linked enzyme aggregates(combi-CLEAs)for one-pot saccharification of sugarcane bagasse[J]. RSC Advances, 2016, 6(39):32849-32857.
[30]Sheldon RA. Characteristicfeatures and biotechnological applications of cross-linked enzyme aggregates(CLEAs)[J].Applied Microbiology and Biotechnology, 2011, 92(3):467-477.
[31]Su E, Meng Y, Ning C, et al. Magnetic combined cross-linked enzyme aggregates(Combi-CLEAs)for cofactor regeneration in the synthesis of chiral alcohol[J]. J Biotechnol, 2018, 271:1-7.
[32]Sóti V, Lenaerts S, Cornet I. Of enzyme use in cost-effective high solid simultaneous saccharification and fermentation processes[J]. J Biotechnol, 2018, 270:70-76.
[33]Singh RL, Singh PK. Singh RP. Enzymatic decolorization and degradation of azo dyes-A review[J]. International Biodeterioration&Biodegradation, 2015, 104:21-31.
[34]Kuma VV, Kumar MPP, Thiruvenkadaravi KV, et al. Preparation and characterization of porous cross linked laccase aggregates for the decolorization of triphenyl methane and reactive dyes[J].Bioresource Technology, 2012, 119(9):28-34.
[35]Vr?anskáM, VoběrkováS, Jiménez AJ, et al. Preparation and optimisation of cross-linked enzyme aggregates using native isolate white rot fungi Trametes versicolor and Fomes fomentarius for the decolourisation of synthetic dyes[J]. International Journal of Environmental Research and Public Health, 2017, 15(1):23.
[36]Kumar VV, Sivanesan S, Cabana H. Magnetic cross-linked laccase aggregates-Bioremediation tool for decolorization of distinct classes of recalcitrant dyes[J]. Science of the Total Environment, 2014,487(1):830-839.
[37]Cabana H, Jones JP, Agathos SN. Utilization of cross-linked laccase aggregates in a perfusion basket reactor for the continuous elimination of endocrine-disrupting chemicals[J]. Biotechnology and Bioengineering, 2009, 102(6):1582-1592.
[38]SinirliogluZA,SinirliogluD,AkbasF.Preparationand characterization of stable cross-linked enzyme aggregates of novel laccase enzyme from Shewanella putrefaciens and using malachite green decolorization[J]. Bioresource Technology, 2013, 146(10):807-811.
[39]Jeong J, Song W, Cooper WJ, et al. Degradation of tetracycline antibiotics:mechanisms and kinetic studies for advanced oxidation/reduction processes[J]. Chemosphere, 2010, 78(5):533-540.
[40]Yang J, Lin Y, Yang X, et al. Degradation of tetracycline byimmobilizedlaccaseandtheproposedtransformation pathway[J]. Journal of Hazardous Materials, 2017, 322:525-531.
[41]Kumar VV, Cabana H. Towards high potential magnetic biocatalysts for on-demand elimination of pharmaceuticals[J]. Bioresource Technology, 2016, 200:81-89.
[42]Jiang Y, Wang Q, He Y, et al. Co-aggregation of laccase and nature egg white:a simple method to prepare stable and recyclable biocatalyst[J]. Applied Biochemistry and Biotechnology, 2014,172(5):2496-2506.
[43]Matijo?yt?I, Arends IWCE, Vries SD, et al. Preparation and use of cross-linked enzyme aggregates(CLEAs)of laccases[J].Journal of Molecular Catalysis B:Enzymatic, 2010, 62(2):142-148.
[44]Nghiem LN, Hai FI, Kang J, et al. Enhancement of trace organic contaminant degradation by crude enzyme extract from Trametes versicolor culture:Effect of mediator type and concentration[J].Journal of the Taiwan Institute of Chemical Engineers, 2014, 45(4):1855-1862.
[45]Chauhan PS, Goradia B, Saxena A. Bacterial laccase:recent update on production, properties and industrial applications[J].3 Biotech, 2017, 7(5):323.