基于气相离子迁移谱和化学计量学方法判别肉的种类
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摘要
建立一种快速、无损分析肉中挥发性有机成分的顶空进样气相色谱-离子迁移谱联用方法,结合化学计量学方法构建快速分类模型以区分肉的种类。以牛肉、羊肉和鸡肉共59个样品为研究对象,称取约1 g待测肉样于标准瓶中,用磁帽密封,直接进行GC-IMS分析。结果表明,GC-IMS三维指纹谱可以有效表征不同肉类之间的气味差异信息,选取对应三维谱中26个特征峰的强度作为变量参数,用PCA方法对数据进行降维分析,并用LDA算法对样品进行分类。结果表明,采用前2个主成分能达到98.3%的正确率,仅有1个牛肉样品被误判成羊肉样品。以顶空进样气相色谱-离子迁移谱检测肉中挥发性成分,用PCA和LDA分析鉴别肉的种类具有可行性。
A rapid and nondestructive headspace gas chromatography tandem ion mobility spectrometry(GC-IMS)method was established for the analysis of the volatile organic compounds in 3 kinds of meat.59 samples were taken as the research object with beef,chicken and lamb,about 1 g tested sample was put into the standard sample bottle and sealed with magnetic cap for direct GC-IMS detection and analysis.The results show that the sample information area of characteristic peaks of GC-IMS 3 D spectra corresponding to volatile organic substances can effectively characterize different types of meat,sectional intensity of area of the corresponding three-dimensional spectrum of 26 characteristic peaks were selected as variables.Principal component analysis(PCA) was used to reduce dimension of the selected variables of three kinds of meat and linear discrimination analysis(LDA) was further used to classify them.The accuracies of 98.3%were obtained by utilizing first two PCs and only 1 beef samples were misjudged as the lamb sample.GC-IMS is simple,rapid and nondestructive technology,and can be used for rapid identification of other food and agricultural products.
A rapid and nondestructive headspace gas chromatography tandem ion mobility spectrometry(GC-IMS)method was established for the analysis of the volatile organic compounds in 3 kinds of meat.59 samples were taken as the research object with beef,chicken and lamb,about 1 g tested sample was put into the standard sample bottle and sealed with magnetic cap for direct GC-IMS detection and analysis.The results show that the sample information area of characteristic peaks of GC-IMS 3 D spectra corresponding to volatile organic substances can effectively characterize different types of meat,sectional intensity of area of the corresponding three-dimensional spectrum of 26 characteristic peaks were selected as variables.Principal component analysis(PCA) was used to reduce dimension of the selected variables of three kinds of meat and linear discrimination analysis(LDA) was further used to classify them.The accuracies of 98.3%were obtained by utilizing first two PCs and only 1 beef samples were misjudged as the lamb sample.GC-IMS is simple,rapid and nondestructive technology,and can be used for rapid identification of other food and agricultural products.
引文
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[3]成黎北.中国肉类产品质量安全现状及改善控制措施[J].食品科学,2010,31(19):465-468.
[4]DING Q,WANG Z Y,HUANG L,et al.Electrical bioimpedance measurement technology and its application in detection of meat quality[J].Transducer&Microsystem Technologies,2009,28(3):4-7.
[5]杨彦杰,昝林森,王洪宝.秦川牛脂联素基因SNPs检测及其与胴体、肉质性状的相关性[J].遗传,2009,31(10):1006-1012.
[6]PENG Y,ZHANG L.Advancement of rapid detection of meat quality and safety by using spectral imaging technology[J].Journal of Food Safety&Quality,2010,27(2):62-72.
[7]PENG Y K,ZHANG H Y.Advancement of nondestructive detection technology of meat quality and safety[J].Food&Nutrition in China,2011,17(10):5-10.
[8]兰冬梅,许平,林晓岚,等.植物油品质指标及其检测的研究进展[J].农产品加工,2015(3):68-70.
[9]ZHANG L,SHUAI Q,LI P,et al.Ion mobility spectrometry fingerprints:A rapid detection technology for adulteration of sesame oil[J].Food Chemistry,2016,192(6):60-66.
[10]KUKLYA A,REINECKE T,UTESCHIL F,et al X-ray ionization differential ion mobility spectrometry[J].Talanta,2017,162(10):159-166.
[11]GRAAFF R J V D.The mobility of ions in gases[J].Nature,1929,124(3114):10-11.
[12]BRADBURY N E,NIELSEN R A.Absolute values of the electron mobility in hydrogen[J].Phys Rev,1936,49(5):388-393.
[13]GALLART-MATEU D,ARMENTA S,DE LAGUARDIA M.Indoor and outdoor determination of pesticides in air by ion mobility spectrometry[J].Talanta,2016,161(10):632-639.
[14]KAFLE G K,KHOT L R,SANKARAN S,et al State of ion mobility spectrometry and applications in agriculture:A review[J].Engineering in Agriculture,Environment and Food,2016,9(4):346-357.
[15]PUTON J,NAMIE S NIK J.Ion mobility spectrometry:Current status and application for chemical warfare agents detection[J].TrAC Trends in Analytical Chemistry,2016,85(Part B):10-20.
[16]彭丽英,王卫国,王新,等.离子迁移谱快速筛查白酒中痕量邻苯二甲酸酯的研究[J].分析化学,2014,42(2):278-282.
[17]常春艳,葛含光,王利强,等.离子迁移谱快速测定食品中熏蒸剂溴甲烷[J].食品研究与开发,2016,37(14):116-119.
[18]冯雪,尹利辉,金少鸿,等.离子迁移谱及其在药学领域的应用[J].药物分析杂志,2013,33(7):1109-1114.
[19]陈轲,颜毅坚,马军,等.离子迁移谱法对地沟油的检测研究[J].中国油脂,2015,40(12):66-69.
[20]钱冲,廖永红,刘明艳,等.不同香型白酒的聚类分析和主成分分析[J].中国食品学报,2017,17(2):243-255.