贮藏年限对燕麦种子生理生化特性的影响
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摘要
为研究贮藏时间对燕麦种子萌发及生理生化特性的影响,本试验以白燕2号和白燕7号为供试材料,研究了燕麦种子萌发特性、渗透调节物质含量、抗氧化酶和脱氢酶活性、丙二醛含量随贮藏年限的变化情况。结果表明,燕麦种子发芽势等萌发指标和幼苗生长均随贮藏时间的延长而下降。贮藏8年时发芽率显著降低,白燕2号低于40%;而白燕7号仍有60%以上。在0～4年的贮藏期内,燕麦种子脯氨酸和可溶性糖含量增加,丙二醛含量显著上升;白燕2号抗氧化物酶活性随贮藏年限的增加显著下降,白燕7号的则明显上升。贮藏8年后燕麦种子渗透调节物质和抗氧化物酶活性急剧下降,有毒物质积累增加,种子贮藏特性变劣。白燕7号的耐贮藏性明显优于白燕2号。
In order to study the effects of storage years on the germination and physiological and biochemical characteristics of oat seeds,two oat varieties(Baiyan No.2 and Baiyan No.7)with different storage years were used as materials to study the germination characteristics,osmotic adjustment substances,antioxidant enzymes and dehydrogenase activities,and malondialdehyde content were tested.The results showed that the seed germination index and seedling growth of germinated oat seed decreased with the increase in storage years.The germination rate decreased significantly after storage for 8 years,Baiyan No.2 was lower than 40%,and Baiyan No.7 was higher than 60%.During storage period of 0～4 years,the content of proline and soluble sugar in oat seeds increased,and the content of malondialdehyde increased significantly.The activity of antioxidant enzymes of Baiyan No.2 decreased significantly with the increase of storage age,while an obvious increment was obtained in Baiyan No.7.After 8 years of storage,the osmotic adjustment substance and antioxidant enzymes activity of oat seed decreased sharply,the accumulation of toxic substances increased,and the storage characteristics of seeds deteriorated.The storage stability of Baiyan No.7 was much better than that of Baiyan No.2.
In order to study the effects of storage years on the germination and physiological and biochemical characteristics of oat seeds,two oat varieties(Baiyan No.2 and Baiyan No.7)with different storage years were used as materials to study the germination characteristics,osmotic adjustment substances,antioxidant enzymes and dehydrogenase activities,and malondialdehyde content were tested.The results showed that the seed germination index and seedling growth of germinated oat seed decreased with the increase in storage years.The germination rate decreased significantly after storage for 8 years,Baiyan No.2 was lower than 40%,and Baiyan No.7 was higher than 60%.During storage period of 0～4 years,the content of proline and soluble sugar in oat seeds increased,and the content of malondialdehyde increased significantly.The activity of antioxidant enzymes of Baiyan No.2 decreased significantly with the increase of storage age,while an obvious increment was obtained in Baiyan No.7.After 8 years of storage,the osmotic adjustment substance and antioxidant enzymes activity of oat seed decreased sharply,the accumulation of toxic substances increased,and the storage characteristics of seeds deteriorated.The storage stability of Baiyan No.7 was much better than that of Baiyan No.2.
引文
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[33]李青莲.不同贮藏时间夏枯草种子萌发特性及播种技术研究[D].郑州:河南农业大学,2017:17-24
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[35]张东晖,云锦凤,石凤敏,等.不同贮藏时间披碱草种子劣变及活力测定[J].草业科学,2008,24(04):116-118
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[37]张虎,寇江涛,师尚礼.紫花苜蓿种子萌发对钴胁迫的生理生化响应[J].草业学报,2015,24(09):146-153
[38]周万海,师尚礼,寇江涛.盐胁迫下外源NO对苜蓿幼苗生长及氮代谢的影响[J].应用生态学报,2012,23(11):3003-3008
[39]杜锦,曹高燚,杨勇,等.人工老化对不同品种小麦种子生理生化特性的影响[J].天津农业科学,2016,22(01):32-36
[40]李西,吴亚娇,孙凌霞.铅胁迫对三种暖季型草坪草生长和生理特性的影响[J].草业学报,2014,23(4):171-180
[41]列淦文,叶龙华,薛摇立.臭氧胁迫对植物主要生理功能的影响[J].生态学报,2014,34(2):294-306
[42]祝煜中,夏黎明,竺思仪,等.人工老化的甜玉米种子活力、生理特性和遗传多样性的变化[J].华南农业大学学报,2018,39(01):25-30
[43]刘志华,王妮,陈立宇,等.罗布麻种子自然状态老化过程中生理生化指标测定与分析[J].种子,2015,34(03):43-46
[44]李玉荣,韩建国,孙彦,等.新麦草种子劣变过程中生理生化变化[J].草地学报,2005,13(3):180-183
[45]孔令琪,王俊峰,刘国庆等.苜蓿硬实种子对人工老化时间处理的生理响应[J].草地学报,2011,19(01):81-85
[46]刘明久,王铁固,陈士林,等.玉米种子人工老化过程中生理特性与种子活力的变化[J].核农学报,2008,22(04):510-513
[47]张海艳.糯玉米种子老化过程中种子活力和生理特性的变化[J].玉米科学,2015,23(01):92-96,102
[48]王仲,邵红,张丽敏,等.荷兰豆种子人工老化过程中生理生化指标的研究[J].中国农学通报,2009,25(03):137-139
[49]Biel W,Bobko K,Maciorowski R.Chemical composition and nutritive value of husked and naked oats grain[J].Journal of Cereal Science,2009,49(3):413-418
[50]吴浩,周青平,颜红波,等.人工老化对两种披碱草属种子生理生化特性的影响[J].草业科学,2014,31(12):2251-2256
[51]李笑蕊,王世霞,幺杨,等.裸燕麦和皮燕麦的营养及功能活性成分对比分析[J].粮油食品科技,2015,23(05):50-54
[2]闫慧芳,夏方山,毛培胜.种子老化及活力修复研究进展[J].中国农学通报,2014,30(03):20-26
[3]周国栋.种子老化对老芒麦种质生理特性及遗传完整性变化的影响[D].北京:中国农业科学院,2012:20-21
[4]Ghive D V,Barabde N P,Pote S R.Seed viability and factors affecting seed storage[J].Asian Journal of Bio Science,2007,2:1-2
[5]Mc Donald M B.Seed deterioration:physiology,repair and assessment[J].Seed Science and Technology,1999,27:177-237
[6]卢军,刘宁.低温和长时间储藏对烟草种子萌发及幼苗抗氧化系统的影响[J].生态科学,2015,34(01):87-90
[7]姜义宝,郑秋红,王成章,等.超干贮藏对菊苣种子活力与抗氧化性的影响[J].草业学报,2009,18(05):93-97
[8]吴浩,周青平,颜红波,等.人工老化对两种披碱草属种子生理生化特性的影响[J].草业科学,2014,31(12):2251-2256
[9]郝阳春.储藏时间对油松种子萌发及生理特征变化的影响[J].防护林科技,2016(03):34-35
[10]张永娟,韩蕊,原烨,等.罗布麻种子老化过程中的生理生化特性[J].草业科学,2011,28(12):2130-2135
[11]龙金飞,郑清岭,杨忠仁,等.不同年限对沙芥属植物种子寿命及贮藏物质的影响[J].种子,2017,36(10):15-20
[12]赵桂琴,师尚礼.青藏高原饲用燕麦研究与生产现状、存在问题与对策[J].草业科学,2004,21(11):17-21
[13]黄华,刘学文,王毅.燕麦主要成分提取方法研究进展[J].食品工业,2011,32(06):94-96
[14]赵丽那.燕麦籽粒富营养基因型的筛选[D].贵阳:贵州大学,2017:1-2
[15]戚向阳,曹少谦,刘合生,等.不同品种燕麦的油脂组成及与其它营养物质相关性研究[J].中国食品学报,2014,14(05):63-71
[16]Pekka L,Katja K,Ilkka L,et al.Effect of heat treatment on lipid stability in processed oats[J].Journal of Cereal Science,2003,37:215-221
[17]Cognat C,Shepherd T,Verrall S R,et al.Comparison of two headspace sampling techniques for the analysis of off-flavour volatiles from oat based products[J].Food Chemistry,2012,34(3):1592-1600
[18]刘国富,张宇龙,李玉龙,等.盐胁迫对5种燕麦种子萌发的影响[J].黑龙江畜牧兽医,2017,60(19):150-152,156
[19]高彩婷,刘景辉,张玉芹,等.短期盐胁迫下燕麦幼苗的生理响应[J].草地学报,2017,25(02):337-343
[20]蔡天革,王鹏,唐凤德.盐胁迫对燕麦种子萌发和幼苗抗氧化酶的影响[J].辽宁大学学报(自然科学版),2016,43(01):74-78
[21]杨苗苗.裸燕麦种子老化处理对其种质资源遗传完整性的影响[D].呼和浩特:内蒙古师范大学,2017:1-43
[22]孔令琪.不同老化处理对燕麦种子生理、蛋白质及抗氧化基因的影响[D].北京:中国农业大学,2015:1-80
[23]韩亮亮,毛培胜.燕麦种子人工加速老化条件的筛选优化[J].种子,2007,27(11):31-34
[24]张治安,陈展宇.植物生理学实验技术[M].长春:吉林大学出版社,2000:100-194
[25]Wang W,He A,Peng S,et al.The Effect of Storage Condition and Duration on the Deterioration of Primed Rice Seeds[J].Front Plant Science,2018,9:172
[26]张苗苗,赵彦,云锦凤,等.老化处理对2种披碱草属牧草种子生理生化的影响[J].种子,2012,31(04):23-27
[27]陈泉竹,毛培胜,孔令琪,等.劣变时间和温度对不同含水量燕麦种子的活力影响[J].种子,2016,35(11):31-35
[28]朱萍,孔令琪,李高等.贮藏温度对不同含水量老芒麦种子生理特性的影响[J].草业学报,2011,20(06):101-108
[29]赵鹏,张轶婷,郝丽珍,等.超干处理对沙芥属蔬菜种子活力及抗氧化代谢的影响[J].中国农业科学,2013,46(02):334-342
[30]余波,杜尚广,罗丽萍.种子活力测定方法[J].中国科学:生命科学,2015,45(7):709-713
[31]高荣岐,张庆春.作物种子学[M].北京:中国农业科技出版社,1997:27-64
[32]陈玲玲,程航,张阳阳,等.不同贮藏年限敖汉苜蓿种子活力及生理特性的研究[J].种子,2017,36(03):23-27,32
[33]李青莲.不同贮藏时间夏枯草种子萌发特性及播种技术研究[D].郑州:河南农业大学,2017:17-24
[34]马向丽,何超,罗富成,等.水引发对不同贮藏年限纳罗克非洲狗尾草种子活力的影响[J].中国草地学报,2017,39(05):16-23
[35]张东晖,云锦凤,石凤敏,等.不同贮藏时间披碱草种子劣变及活力测定[J].草业科学,2008,24(04):116-118
[36]李培英,孙宗玖,阿不来提,等.常温贮藏下新农1号狗牙根种子劣变的研究[J].草业科学,2007,23(10):63-67
[37]张虎,寇江涛,师尚礼.紫花苜蓿种子萌发对钴胁迫的生理生化响应[J].草业学报,2015,24(09):146-153
[38]周万海,师尚礼,寇江涛.盐胁迫下外源NO对苜蓿幼苗生长及氮代谢的影响[J].应用生态学报,2012,23(11):3003-3008
[39]杜锦,曹高燚,杨勇,等.人工老化对不同品种小麦种子生理生化特性的影响[J].天津农业科学,2016,22(01):32-36
[40]李西,吴亚娇,孙凌霞.铅胁迫对三种暖季型草坪草生长和生理特性的影响[J].草业学报,2014,23(4):171-180
[41]列淦文,叶龙华,薛摇立.臭氧胁迫对植物主要生理功能的影响[J].生态学报,2014,34(2):294-306
[42]祝煜中,夏黎明,竺思仪,等.人工老化的甜玉米种子活力、生理特性和遗传多样性的变化[J].华南农业大学学报,2018,39(01):25-30
[43]刘志华,王妮,陈立宇,等.罗布麻种子自然状态老化过程中生理生化指标测定与分析[J].种子,2015,34(03):43-46
[44]李玉荣,韩建国,孙彦,等.新麦草种子劣变过程中生理生化变化[J].草地学报,2005,13(3):180-183
[45]孔令琪,王俊峰,刘国庆等.苜蓿硬实种子对人工老化时间处理的生理响应[J].草地学报,2011,19(01):81-85
[46]刘明久,王铁固,陈士林,等.玉米种子人工老化过程中生理特性与种子活力的变化[J].核农学报,2008,22(04):510-513
[47]张海艳.糯玉米种子老化过程中种子活力和生理特性的变化[J].玉米科学,2015,23(01):92-96,102
[48]王仲,邵红,张丽敏,等.荷兰豆种子人工老化过程中生理生化指标的研究[J].中国农学通报,2009,25(03):137-139
[49]Biel W,Bobko K,Maciorowski R.Chemical composition and nutritive value of husked and naked oats grain[J].Journal of Cereal Science,2009,49(3):413-418
[50]吴浩,周青平,颜红波,等.人工老化对两种披碱草属种子生理生化特性的影响[J].草业科学,2014,31(12):2251-2256
[51]李笑蕊,王世霞,幺杨,等.裸燕麦和皮燕麦的营养及功能活性成分对比分析[J].粮油食品科技,2015,23(05):50-54
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