顶花板凳果耐寒性分析及评价指标的筛选
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摘要
以2年生顶花板凳果为材料,研究了不同的低温处理时间对其叶片MDA含量、相对电导率、可溶性蛋白含量、SOD活性、叶绿素含量以及钙离子浓度的影响,采用相关性分析和主成分分析法对各生理指标进行了分析和比较。结果表明,板凳果在低温处理后,叶片卷曲,颜色呈深绿色。随着低温的持续,板凳果MDA含量、可溶性蛋白含量、叶绿素a含量、叶绿素b含量、类胡萝卜素含量、叶绿素总量、SOD活性和相对电导率均呈上升趋势,在低温处理96 h时,各含量均达到最大值,且与对照间差异显著;钙离子浓度在处理48 h时达到最大值,显著高于对照。通过相关性和主成分分析得出,类胡萝卜素含量、可溶性蛋白含量、叶绿素a含量、叶绿素总量、相对电导率和叶绿素b含量为较大特征向量,系数分别为0.991,0.985,0.983,0.967,0.956和0.907,其与顶花板凳果抗寒性关系密切。
Taking 2-year-old Pachysandra terminalis as test materials, the effect of different low temperature treatment time on MDA content, relative electric conductivity, soluble protein content, SOD activity, chlorophyll content and Ca2+concentration were studied. Correlative analysis and principal component analysis were used to analyze and compare the physiological indexes. The results showed that Pachysandra terminalis leaves curled, and were dark green in color after low temperature treatment. With the continued low temperature, the MDA content, soluble protein content, chlorophyll a content, chlorophyll b content, carotenoid content, total chlorophyll content, SOD activity and relative electric conductivity increased, and when the low temperature was treated for 96 h, all contents reached the maximum value, which was significantly difference with the control. Ca2+concentration reached a maximum at 48 h of treatment, was significantly higher than the control. Correlative analysis and principal component analysis showed that the correlations between carotenoid content, soluble protein content, chlorophyll a content, total chlorophyll content, relative electrical conductivity and chlorophyll b content were larger eigenvectors, the coefficients were 0.991, 0.985, 0.983, 0.967, 0.956 and 0.907, and it was closely related to the cold resistance of the Pachysandra terminalis.
Taking 2-year-old Pachysandra terminalis as test materials, the effect of different low temperature treatment time on MDA content, relative electric conductivity, soluble protein content, SOD activity, chlorophyll content and Ca2+concentration were studied. Correlative analysis and principal component analysis were used to analyze and compare the physiological indexes. The results showed that Pachysandra terminalis leaves curled, and were dark green in color after low temperature treatment. With the continued low temperature, the MDA content, soluble protein content, chlorophyll a content, chlorophyll b content, carotenoid content, total chlorophyll content, SOD activity and relative electric conductivity increased, and when the low temperature was treated for 96 h, all contents reached the maximum value, which was significantly difference with the control. Ca2+concentration reached a maximum at 48 h of treatment, was significantly higher than the control. Correlative analysis and principal component analysis showed that the correlations between carotenoid content, soluble protein content, chlorophyll a content, total chlorophyll content, relative electrical conductivity and chlorophyll b content were larger eigenvectors, the coefficients were 0.991, 0.985, 0.983, 0.967, 0.956 and 0.907, and it was closely related to the cold resistance of the Pachysandra terminalis.
引文
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[2]马祥瑞,焦丽华,朱永强.顶花板凳果特性及繁育应用[J].农业科技与信息,2016(31):129.
[3]何士洋,沈超强,顾亮,等.优良耐阴地被植物富贵草的繁殖及园林应用[J].现代农业科技,2017(16):144.
[4]王安敏,郝德民.常绿耐荫地被植物——顶花三角咪在北京引种成功[J].中国花卉盆景,1993(3):25.
[5]马勋.介绍几种北方常绿阔叶植物[N].中国花卉报,2003-01-18.
[6]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:105-263.
[7]沈伟其.测定水稻叶片叶绿素含量的混合液提取法[J].植物生理学通讯,1988(3):62-64.
[8]GONG M,DING N C,HE Z Y,et al. Correlation between lipid pre-oxidation damage and ultrastruc-tural changes of mesophyll cells inbarley and wheat seedling during salt stress[J]. Journal of IntegrativePlant Biology,1989,31(11):841-846.
[9]BRADFORD M M. A rapid and sensitive method for the quantifica-tion of microgram quantities of protein in utilizing the principle ofprotein-dye binding[J]. Analytical Biochemistry,1976,72(7):248-254.
[10]王兆.低温胁迫对彩叶草的生理效应及抗寒性研究[D].福州:福建农林大学,2014.
[11]陈明辉,程世平,张志录,等.不同品种果蔗幼苗对低温的生理响应及耐寒性评价[J].华南农业大学学报,2018,39(2):40-46.
[12]张军,张文,张贝贝,等. 5个冬小麦品种(系)的越冬生理特性[J].贵州农业科学,2018,46(1):11-14.
[13]李钊.玉米苗期抗冻生理响应及其转录组调控分析[D].哈尔滨:东北农业大学,2017.
[14]石如意,王腾飞,李军,等.低温胁迫下外源ABA对玉米幼苗抗寒性的影响[J].华北农学报,2018,33(3):136-143.
[15]张迎辉.低温胁迫下福建山樱花的生理响应与抗寒基因的表达[D].福州:福建农林大学,2014.
[16]曹颖,金刚,刘明国.阜新地区引种金银花抗寒生理指标对比分析[J].辽宁林业科技,2009(4):23-26.
[17]刘冰.花椒抗寒性研究[D].兰州:甘肃农业大学,2005.
[18]赵跃锋,陈昆,张清华.盐胁迫对茄子光合特性、叶绿素荧光及保护酶活性的影响[J].山西农业科学,2018,46(11):1797-1799,1906.
[19]崔青云.金钱松(Pseudolarix kaempferi)抗寒抗旱性研究[D].杭州:浙江农林大学,2011.
[20]严海燕.常绿阔叶灌木富贵草的生态适应性研究[D].长春:吉林农业大学,2013.
[21]李长慧,李淑娟,刘艳霞,等.低温胁迫对10份鹅观草属野生种质抗寒生理指标的影响[J].草业科学,2018,35(1):123-132.
[22]杜春芳.甘蓝型油菜低温诱导的转录组和蛋白组分析[D].武汉:华中农业大学,2016.
[23]郭爱华,左宝峰,姚延梼,等.自然降温对雪松叶片中叶绿素及电导率的影响[J].山西农业大学学报(自然科学版),2005(4):393-395.
[24]何玮,王家兵,张丽,等.外源钙离子对低温胁迫下扁穗牛鞭草叶片保护酶活性的影响[J].中国农学通报,2015,31(12):175-180.
[25]田家明,张智猛,戴良香,等.外源钙对盐碱土与非盐碱土花生生长发育与光合特性的影响[J].华北农学报,2018,33(6):130-136.
[26] RASMUSSED H,BARRETT P Q. Calcium messenger system:anintegrated view[J]. Physiol Rev,1984,64:938-984.
[27]刘学琴.钙在低温胁迫三尖杉过程中信号转导机制研究[D].福州:福建农林大学,2006.