高效液相色谱法测定磺胺间甲氧嘧啶的含量
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摘要
建立了兽药磺胺间甲氧嘧啶钠注射液中磺胺间甲氧嘧啶含量的高效液相色谱测定法。以C18为固定相,0.1%磷酸溶液-乙腈(80∶20)为流动相,流速1.0 m L/min,柱温40℃,检测波长270 nm,采用外标法进行定量。磺胺间甲氧嘧啶的线性范围为5.0~100.0μg/m L,回归方程为Area=40.333×Amt-18.843(n=5),相关系数大于0.999。从高(120%)、中(100%)和低(80%)三个添加水平的检测结果可以看出,磺胺间甲氧嘧啶的回收率为96.27%~98.30%。该方法简单,准确,精密度好,回收率高,可以用于测量磺胺间甲氧嘧啶钠注射液中磺胺间甲氧嘧啶的含量。
An HPLC method has been developed to determine the content of sulfamonomethoxine in sulfamonomethoxine sodium injection,using C18 column as stationary phase,with a mobile phase of 0. 1%phosphoric solution-acetonitrile( 80 ∶ 20) and wavelength of 270 nm. It was quantified by external standard method and the linear range of sulfamonomethoxine was between 5. 0 and 100. 0 μg/m L. And the regression equation was Area = 40. 333 × Amt-18. 843( n = 5) with the correlation to be more than 0. 999. The average recoveries at three different levels of 120%,100% and 80% of the sample ranged between 96. 27% and98. 30%. This method was simple and accurate,and had a good precision and high recoveries as well,could be used for determination of sulfamonomethoxine in sulfamonomethoxine sodium injection.
An HPLC method has been developed to determine the content of sulfamonomethoxine in sulfamonomethoxine sodium injection,using C18 column as stationary phase,with a mobile phase of 0. 1%phosphoric solution-acetonitrile( 80 ∶ 20) and wavelength of 270 nm. It was quantified by external standard method and the linear range of sulfamonomethoxine was between 5. 0 and 100. 0 μg/m L. And the regression equation was Area = 40. 333 × Amt-18. 843( n = 5) with the correlation to be more than 0. 999. The average recoveries at three different levels of 120%,100% and 80% of the sample ranged between 96. 27% and98. 30%. This method was simple and accurate,and had a good precision and high recoveries as well,could be used for determination of sulfamonomethoxine in sulfamonomethoxine sodium injection.
引文
[1]中国兽药典委员会.中华人民共和国兽药典(二部)[M].二O一O年版.北京:中国农业出版社.
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[3]中国兽医药品监察所.中国兽医药品监察所兽药残留检验操作规程CJCZ020[Z].
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[5]耿士伟,曲斌,姜加华,等.Qu ECh ERS-UPLC-MS/MS快速测定鸡肝中七种磺胺类药物残留[J].中国兽药杂志,2011,45(10):16-19.
[6]查玉兵,陈美,王晓芳,等.高效液相色谱法测定猪肉中五种磺胺类药物残留量[J].分析仪器,2008,(4):44-45.
[7]陈锡龙.HPLC法测定复方磺胺对甲氧嘧啶片中磺胺对甲氧嘧啶的含量[J].中国兽药杂志,2014,48(7):30-33.
[8]杨武宁,崔艳莉.HPLC法测定复方磺胺对甲氧嘧啶钠可溶性粉含量[J].中国动物保健,2013,15(8):30-32.
[2]农业部兽药评审中心,兽药国家标准汇编——兽药地方标准上升国家标准(第一册)[M].北京:中国农业出版社.2010:87-90
[3]中国兽医药品监察所.中国兽医药品监察所兽药残留检验操作规程CJCZ020[Z].
[4]中华人民共和国农业部.农牧发[2001]38号文件[S].
[5]耿士伟,曲斌,姜加华,等.Qu ECh ERS-UPLC-MS/MS快速测定鸡肝中七种磺胺类药物残留[J].中国兽药杂志,2011,45(10):16-19.
[6]查玉兵,陈美,王晓芳,等.高效液相色谱法测定猪肉中五种磺胺类药物残留量[J].分析仪器,2008,(4):44-45.
[7]陈锡龙.HPLC法测定复方磺胺对甲氧嘧啶片中磺胺对甲氧嘧啶的含量[J].中国兽药杂志,2014,48(7):30-33.
[8]杨武宁,崔艳莉.HPLC法测定复方磺胺对甲氧嘧啶钠可溶性粉含量[J].中国动物保健,2013,15(8):30-32.