基于不可逆电穿孔肿瘤阻抗变化与肿瘤灭活效果关系的研究
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摘要
研究不可逆电穿孔对生物肿瘤细胞的影响,利用动物实验方法分析不可逆电穿孔时肿瘤组织的阻抗分布.对乳腺癌组织小鼠进行实验,脉冲参数为100 0 V,脉宽70μs,占空比50%,每组脉冲数10,共10组.实验结果表明:在不可逆电穿孔情况下,阻抗在一定的斜率范围内减小,通过算法计算,阻抗的斜率在-0. 091×10~(-3)和-1. 474×10~(-3)之间.在电脉冲刺激肿瘤过程中,可以将被刺激区域电阻抗的变化率作为反馈信息,实时有效地验证不可逆电穿孔效果,提升肿瘤灭活效率,为不可逆电穿孔技术在临床应用提供新的研究思路.
In order to study the effect of irreversible electroporation on biological tumor cells,animal experiments were used to analyze the impedance distribution of tumor tissue during irreversible electroporation. The experiments were performed on mouse with breast cancer tissue under the following con: the voltage pulse parameter is 1 000 V,pulse width is 70 μs,duty cycle is 50%,pulse number is 10 per group,and the group number is 10. The experimental results show that the impedance decreases in a certain slope range under the condition of irreversible electroporation,and the slope of the impedance is calculated by the algorithm between -0. 091 × 10~(-3) and-1. 474 × 10~(-3). In the process of electric pulse stimulation of the tumor,the change rate of electrical impedance of the stimulated area can be used as feedback information to effectively verify the irreversible electroporation effect in real time. It can enhance the efficiency of the tumor inactivation and provide new research ideas for clinical application of irreversible electroporation technology.
In order to study the effect of irreversible electroporation on biological tumor cells,animal experiments were used to analyze the impedance distribution of tumor tissue during irreversible electroporation. The experiments were performed on mouse with breast cancer tissue under the following con: the voltage pulse parameter is 1 000 V,pulse width is 70 μs,duty cycle is 50%,pulse number is 10 per group,and the group number is 10. The experimental results show that the impedance decreases in a certain slope range under the condition of irreversible electroporation,and the slope of the impedance is calculated by the algorithm between -0. 091 × 10~(-3) and-1. 474 × 10~(-3). In the process of electric pulse stimulation of the tumor,the change rate of electrical impedance of the stimulated area can be used as feedback information to effectively verify the irreversible electroporation effect in real time. It can enhance the efficiency of the tumor inactivation and provide new research ideas for clinical application of irreversible electroporation technology.
引文
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[10]卢强,闫小龙,黄立军,等.高压电场不可逆电穿孔诱发A549肺癌细胞凋亡的实验研究[J].现代生物医学进展,2014,14(3):425-428.
[11] GOLDMAN D E. Potential impedance and rectification in membranes[J]. The Journal of General Physiology,1943,27(1):37-60.
[12] COASTER H G L. A quantitative analysis of the voltagecurrent relationships of fixed charge membranes and the associated property of"punch-through"[J]. Biophysical Journal,1965,5(5):669-686.
[13] PAVLING M,Kanduser M,Rebersek M. Effect of cell electroporation on the conductivity of a cell suspension[J]. Biophysical journal,2005,88(6):4378-4390.
[14] OINK G,MIKUS P,RUBINSKY B. Irreversible electroporation:implications for prostate ablation[J]. Technology in Cancer Research and Treatment,2007,6(4):295-300.
[15] IVORRA A,RUBINSKY B. In vivo electrical impedance measurements during and after electroporation of rat liver[J]. Bioelectrochemistry,2007,70(2):287-295.
[16] IVORRA A,AI-SAKERE B,RUBINSKY B. In vivo electrical conductivity measurements during and after tumor electroporation:conductivity changes reflect the treatment outcome[J]. Physics in Medicine and Biology,2009,54(19):5949-5963.
[17] RUBINSKY Y,IVORRA A,MAOR E. In vivo imaging of irreversible electroporation by means of electrical impedance tomography[J]. Physics in Medicine and Biology,2009,54(16),4927-4943.
[18]董秀珍.生物电阻抗技术研究进展[J].物理学报,2004,(6),311-317.
[2] DAVALOS R V,MIR I L,RUBINSKY B. Tissue ablation with irreversible electroporation[J]. Annals of Biomedical Engineering,2005,33(2):223-231.
[3] NEUMANN E,SCHAEFER-RIDDER M,WANG Y.Gene transfer into mouse lyoma cells by electroporation in high electric fields[J]. The EMBO Journal,1982,1(7):841-845.
[4] BAMES F S G B. Handbook of biological effects of electromagnetic fields(CRC/Taylor&Francis,Boca Raton[J]. Biophysical Journal,2007,5(3):47-57.
[5] WEAVER J C. Electroporation:A general phenomenon for manipulating cells and tissues[J]. Transactions on Plasma Science,2000,28(1):24-33.
[6] EDD J F,HOROWITZ J,DAVALOS R V. In vivo results of a new focal tissue ablation technique:irreversible electroporation[J]. Transactions on Biomedical Engineering,2006,53(7):1409-1415.
[7] MAOR E,IVORRA A,LEOR J. The effect of irreversible electroporation on blood vessels[J]. Technology in Cancer Research and Treatment,2007,6(4):307-312.
[8] MAOR E,IVORRA A,RUBINSKY B. Non thermal irreversible electroporation:novel technology for vascular smooth muscle cells ablation[J]. Plos One,2009,4(3):4757.
[9] MIKLAVCIC D,SEMROV D,MEKID H. A validated model of in vivo electric field distribution in tissues for electrochemotherapy and for DNA electrotransfer for gene therapy[J]. Biochimica et Biophysica Acta,2004,1523(1):73-83.
[10]卢强,闫小龙,黄立军,等.高压电场不可逆电穿孔诱发A549肺癌细胞凋亡的实验研究[J].现代生物医学进展,2014,14(3):425-428.
[11] GOLDMAN D E. Potential impedance and rectification in membranes[J]. The Journal of General Physiology,1943,27(1):37-60.
[12] COASTER H G L. A quantitative analysis of the voltagecurrent relationships of fixed charge membranes and the associated property of"punch-through"[J]. Biophysical Journal,1965,5(5):669-686.
[13] PAVLING M,Kanduser M,Rebersek M. Effect of cell electroporation on the conductivity of a cell suspension[J]. Biophysical journal,2005,88(6):4378-4390.
[14] OINK G,MIKUS P,RUBINSKY B. Irreversible electroporation:implications for prostate ablation[J]. Technology in Cancer Research and Treatment,2007,6(4):295-300.
[15] IVORRA A,RUBINSKY B. In vivo electrical impedance measurements during and after electroporation of rat liver[J]. Bioelectrochemistry,2007,70(2):287-295.
[16] IVORRA A,AI-SAKERE B,RUBINSKY B. In vivo electrical conductivity measurements during and after tumor electroporation:conductivity changes reflect the treatment outcome[J]. Physics in Medicine and Biology,2009,54(19):5949-5963.
[17] RUBINSKY Y,IVORRA A,MAOR E. In vivo imaging of irreversible electroporation by means of electrical impedance tomography[J]. Physics in Medicine and Biology,2009,54(16),4927-4943.
[18]董秀珍.生物电阻抗技术研究进展[J].物理学报,2004,(6),311-317.
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