西藏皱皮木瓜中齐墩果酸、熊果酸提取工艺优化及不同干燥方式的含量分析
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摘要
本研究旨在建立齐墩果酸和熊果酸同时分析方法,并测定西藏木瓜在不同干燥方式下西藏木瓜中齐墩果酸和熊果酸含量。选用甲醇为提取溶剂,超声波辅助浸提,通过单因素实验及响应面优化齐墩果酸、熊果酸提取工艺,采用高效液相色谱法检测西藏皱皮木瓜在冷冻干燥、自然晾干、恒温烘干三种不同干燥方式下齐墩果酸、熊果酸含量。结果表明,西藏皱皮木瓜齐墩果酸、熊果酸最佳提取工艺条件:以甲醇为提取溶剂,60 W功率的超声波辅助提取、提取时间55 min、提取温度59℃,齐墩果酸和熊果酸提取率分别为0.2766±0.01591%,0.5705±0.0563%;利用本文所建立的提取工艺及分析方法,检测到西藏皱皮木瓜熊果酸和齐墩果酸含量大小顺序为:鲜样品﹥冷冻干燥﹥自然晾干﹥50℃恒温烘干﹥60℃恒温烘干﹥70℃恒温烘干。结果说明,冷冻干燥最有利保存西藏皱皮木瓜中的熊果酸和齐墩果酸。
This experiment was to optimize the extracting technology of oleanolic acid and ursolic acid from Fructus Chaenomeles and analyze the content in different drying methods. Response surface methodology basing on single factor was used to optimize oleanolic acid and ursolic acid through detecting the targeted component index by high performance liquid chromatography(HPLC). In addition, the methanol was used as extracting solvent and ultrasonic was applied as auxiliary method. The results showed that the best condition for extraction was 60 W ultrasonic,55 min extracting time and 59℃ extracting temperature. The yield rate of oleanolic acid was0.02766±0.001591% and ursolic acid was 0.05705±0.000563%. The contents of oleanolic acid and ursolic acid from Fructus Chaenomeles samples by applying freeze-dried was the highest,then decreased by air-dried with 50℃ constant temperature, by 60℃ constant temperature dried and by 70℃ constant temperature dried. These results indicated that freeze-drying could preserve oleanolic acid and ursolic acid in Fructus Chaenomeles as far as possible.
This experiment was to optimize the extracting technology of oleanolic acid and ursolic acid from Fructus Chaenomeles and analyze the content in different drying methods. Response surface methodology basing on single factor was used to optimize oleanolic acid and ursolic acid through detecting the targeted component index by high performance liquid chromatography(HPLC). In addition, the methanol was used as extracting solvent and ultrasonic was applied as auxiliary method. The results showed that the best condition for extraction was 60 W ultrasonic,55 min extracting time and 59℃ extracting temperature. The yield rate of oleanolic acid was0.02766±0.001591% and ursolic acid was 0.05705±0.000563%. The contents of oleanolic acid and ursolic acid from Fructus Chaenomeles samples by applying freeze-dried was the highest,then decreased by air-dried with 50℃ constant temperature, by 60℃ constant temperature dried and by 70℃ constant temperature dried. These results indicated that freeze-drying could preserve oleanolic acid and ursolic acid in Fructus Chaenomeles as far as possible.
引文
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[5]王志芳.皱皮木瓜中齐墩果酸和熊果酸测定,提取及抗肿瘤活性研究[D].华中农业大学,2006.
[6]Tokuda H,Ohigashi H,Koshimizu K,et al.Inhibitory effects of ursolic and oleanolic acid on skin tumor promotion by12-O-tetradecanoylphorbol-13-acetate[J].Cancer Letters.1986,33(3):279-285.
[7]Hsu H Y,Yang J J,Lin C C.Effects of oleanolic acid and ursolic acid on inhibiting tumor growth and enhancing the recovery of hematopoietic system postirradiation in mice[J].Cancer Letters.1997,111(1-2):7-13.
[8]Jie L I,Guo W J,Yang Q Y.Effects of ursolic acid and oleanolic acid on human colon carcinoma cell line HCT15[J].World Journal of Gastroenterology.2002,8(3):493.
[9]齐红,秦华,郭庆梅.皱皮木瓜组分含量测定及药理作用研究进展[J].中国执业药师.2016(08):39-42.
[10]汪倪萍.木瓜苷对大鼠佐剂性关节炎的治疗作用[J].中国药理学通报.2003,19(3):340-344.
[11]Somova L O,Nadar A,Rammanan P,et al.Cardiovascular,antihyperlipidemic and antioxidant effects of oleanolic and ursolic acids in experimental hypertension[J].Phytomedicine.2003,10(3):115-121.
[12]Yang J J,Gong Y,Shi J,et al.[Study on the effect of ursolic acid(UA)on the myocardial fibrosis of experimental diabetic mice][J].Chinese journal of applied physiology.2013,29(4):353.
[13]王立新,韩广轩,刘文庸等.齐墩果酸的化学及药理研究[J].药学实践杂志.2001(02):104-107.
[14]Laszczyk M N.Pentacyclic Triterpenes of the Lupane,Oleanane and Ursane Group as Tools in Cancer Therapy[J].Planta Medica.2009,75(15):1549-1560.
[15]王岱杰,王晓,耿岩玲等.反相高效液相色谱测定不同品种皱皮木瓜中齐墩果酸和熊果酸含量[J].食品科学.2008(10):497-499.
[16]刘世尧.不同产区皱皮木瓜有机酸组成及主要活性成分分离纯化研究[D].西南大学,2012.
[17]蒋顶云,蒋长兴,熊清平等.皱皮木瓜中齐墩果酸的超临界CO2提取工艺优选[J].中国实验方剂学杂志.2012(17):20-24.
[18]蔡娟,刘世尧,韦正鑫等.皱皮木瓜皮渣齐墩果酸和熊果酸提取工艺优化研究[J].食品工业科技.2015(02):282-285.
[19]李琼霞,李慧.光皮木瓜与皱皮木瓜中齐墩果酸含量比较[J].中国民族民间医药.2009(13):19-20.
[20]张恒,耿岩玲,王岱杰等.不同品种皱皮木瓜的营养成分研究[J].山东科学.2011(02):24-27.
[21]邓才富,罗川,任星宇等.不同采收期皱皮木瓜中熊果酸,齐墩果酸含量比较[J].亚太传统医药.2016(08):30-31.
[22]邢雅丽.泡桐叶中熊果酸的超声波提取与分离研究[D].中国林业科学研究院,2014.
[23]李胜华,伍贤进,郁建平等.超声波提取翻白草中熊果酸工艺优化研究[J].食品科学.2007(08):160-164.
[2]倪志诚.西藏经济植物[M].北京:北京科学技术出版社,1990.
[3]刘厚佳,胡晋红,孙莲娜等.木瓜中齐墩果酸抗乙型肝炎病毒研究[J].解放军药学学报.2002,18(5):272-274.
[4]孔祥密.垂丝海棠和贴梗海棠抗氧化和保肝作用研究[D].河南大学,2014.
[5]王志芳.皱皮木瓜中齐墩果酸和熊果酸测定,提取及抗肿瘤活性研究[D].华中农业大学,2006.
[6]Tokuda H,Ohigashi H,Koshimizu K,et al.Inhibitory effects of ursolic and oleanolic acid on skin tumor promotion by12-O-tetradecanoylphorbol-13-acetate[J].Cancer Letters.1986,33(3):279-285.
[7]Hsu H Y,Yang J J,Lin C C.Effects of oleanolic acid and ursolic acid on inhibiting tumor growth and enhancing the recovery of hematopoietic system postirradiation in mice[J].Cancer Letters.1997,111(1-2):7-13.
[8]Jie L I,Guo W J,Yang Q Y.Effects of ursolic acid and oleanolic acid on human colon carcinoma cell line HCT15[J].World Journal of Gastroenterology.2002,8(3):493.
[9]齐红,秦华,郭庆梅.皱皮木瓜组分含量测定及药理作用研究进展[J].中国执业药师.2016(08):39-42.
[10]汪倪萍.木瓜苷对大鼠佐剂性关节炎的治疗作用[J].中国药理学通报.2003,19(3):340-344.
[11]Somova L O,Nadar A,Rammanan P,et al.Cardiovascular,antihyperlipidemic and antioxidant effects of oleanolic and ursolic acids in experimental hypertension[J].Phytomedicine.2003,10(3):115-121.
[12]Yang J J,Gong Y,Shi J,et al.[Study on the effect of ursolic acid(UA)on the myocardial fibrosis of experimental diabetic mice][J].Chinese journal of applied physiology.2013,29(4):353.
[13]王立新,韩广轩,刘文庸等.齐墩果酸的化学及药理研究[J].药学实践杂志.2001(02):104-107.
[14]Laszczyk M N.Pentacyclic Triterpenes of the Lupane,Oleanane and Ursane Group as Tools in Cancer Therapy[J].Planta Medica.2009,75(15):1549-1560.
[15]王岱杰,王晓,耿岩玲等.反相高效液相色谱测定不同品种皱皮木瓜中齐墩果酸和熊果酸含量[J].食品科学.2008(10):497-499.
[16]刘世尧.不同产区皱皮木瓜有机酸组成及主要活性成分分离纯化研究[D].西南大学,2012.
[17]蒋顶云,蒋长兴,熊清平等.皱皮木瓜中齐墩果酸的超临界CO2提取工艺优选[J].中国实验方剂学杂志.2012(17):20-24.
[18]蔡娟,刘世尧,韦正鑫等.皱皮木瓜皮渣齐墩果酸和熊果酸提取工艺优化研究[J].食品工业科技.2015(02):282-285.
[19]李琼霞,李慧.光皮木瓜与皱皮木瓜中齐墩果酸含量比较[J].中国民族民间医药.2009(13):19-20.
[20]张恒,耿岩玲,王岱杰等.不同品种皱皮木瓜的营养成分研究[J].山东科学.2011(02):24-27.
[21]邓才富,罗川,任星宇等.不同采收期皱皮木瓜中熊果酸,齐墩果酸含量比较[J].亚太传统医药.2016(08):30-31.
[22]邢雅丽.泡桐叶中熊果酸的超声波提取与分离研究[D].中国林业科学研究院,2014.
[23]李胜华,伍贤进,郁建平等.超声波提取翻白草中熊果酸工艺优化研究[J].食品科学.2007(08):160-164.
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