益智仁-乌药醚提取物对糖尿病肾病小鼠p27kip1蛋白表达的影响
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摘要
目的:观察益智仁-乌药醚提取物对糖尿病肾病小鼠p27kip1蛋白表达的影响。方法:应用STZ造模,建立糖尿病肾病模型小鼠,分别给予厄贝沙坦10mg/(kg·d)、益智仁-乌药醚提取物3个剂量,即20g/(kg·d)、10g/(kg·d)、5g/(kg·d)治疗4周,采用RT-PCR和Western blotting方法观察各组小鼠p27kip1蛋白的表达情况。结果:与正常对照组比较,糖尿病肾病模型组p27kip1蛋白表达明显上调;与模型组比较,益智仁-乌药醚提取物20g/kg组和厄贝沙坦组p27kip1蛋白表达均明显下调。结论:益智仁-乌药醚提取物对糖尿病肾病小鼠肾脏有一定的保护作用,其机制可能与调节p27kip1蛋白表达有关。
Objective: To observe the influence of ether extract of the Yizhiren-Wuyao on p27kip1 Protein Expression in diabetic nephropathy mice.Methods: STZ was applied to establish the model of diabetic nephropathy mice. Diabetic nephropathy mice were given positive control drug irbesartan 10mg/( kg·d),ether extract of the Yizhiren-Wuyao three intervendonal 20 g/( kg·d),10 g/( kg·d),5g/( kg·d) for 4 weeks.The expression of p27kip1 Protein was observed by RT-PCR and Western blotting method. Results: Compared with the normal control group,diabetic nephropathy increased expression of p27kip1 Protein in the model group( P < 0. 01); compared with the model group,in the ether extract of the Yizhiren-Wuyao 20g/kg group and irbesartan group p27kip1 Protein expressions significantly decreased( P < 0. 01). Conclusion:ether extract of the Yizhiren-Wuyao on kidney of diabetic nephropathy mice has a protective effect,its mechanism may be related to regulating the expression of p27kip1 protein.
Objective: To observe the influence of ether extract of the Yizhiren-Wuyao on p27kip1 Protein Expression in diabetic nephropathy mice.Methods: STZ was applied to establish the model of diabetic nephropathy mice. Diabetic nephropathy mice were given positive control drug irbesartan 10mg/( kg·d),ether extract of the Yizhiren-Wuyao three intervendonal 20 g/( kg·d),10 g/( kg·d),5g/( kg·d) for 4 weeks.The expression of p27kip1 Protein was observed by RT-PCR and Western blotting method. Results: Compared with the normal control group,diabetic nephropathy increased expression of p27kip1 Protein in the model group( P < 0. 01); compared with the model group,in the ether extract of the Yizhiren-Wuyao 20g/kg group and irbesartan group p27kip1 Protein expressions significantly decreased( P < 0. 01). Conclusion:ether extract of the Yizhiren-Wuyao on kidney of diabetic nephropathy mice has a protective effect,its mechanism may be related to regulating the expression of p27kip1 protein.
引文
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3尹德辉,韦祎,谢毅强,等."益智-乌药"药对抗糖尿病肾病有效部位的筛选.时珍国医国药,2015;26(3)∶579~581
4徐叔云.药理实验方法学.北京:人民卫生出版社.第三版,2005∶1516
5 Schernthaner G,Schernthaner GH.Diabetic nephropathy:new approache for improving glycemic control and reducing risk.J Nephrol,2013;26(6)∶975~985
6 Wolf G,Schanze A,Stahl R,et al.P27kip1knockout mice are pro-tected from diabetic nephropathy:Evidence for p27kip1 haplotype insufficiency.Kidney Int,2005;68(4)∶1583~1589
7 Liu M1,Zhang Y,Chi Y,et al.Delivery of megsin si RNA plasmidreveal therapeutic potential against diabetic nephropathy by down-regulating p27(kip1)level.J Nephrol,2012;25(3)∶418~425
8李俊美.吕仁和教授治疗糖尿病肾病的经验.四川中医,2009;27(5)∶1~3
9赵良斌,李明权,何玉华,等.叶传蕙教授治疗糖尿病肾病用药经验.中华中医药杂志,2013;28(6)∶1764~1767
10陈萍,王培培,焦泽沼,等.益智仁的化学成分及药理活性研究进展.现代药物与临床,2013;28(4)∶617~623
11谢毅强,刘嫱,韦祎,等.益智提取物抗糖尿病肾病有效部位的筛选.中国实验方剂学杂志,2015;21(3)∶114~117
12刘嫱,房磊臣,韦祎,等.益智仁提取物对糖尿病肾病模型小鼠的疗效及机制研究.中国医药导报,2015;12(33)∶21~25
13闫利利,王金芳,李秋月,等.乌药的化学成分研究.中国药业,2013;22(2)∶16~17
2 Liao YC,Lee YH,Chuang LY,et al.Advanced glycation end productsmediated hypertrophy is negatively regulated by tetrahydrobiopterin in rena tubular cells.Mol Cell Endocrinol,2012;355(1)∶71~77
3尹德辉,韦祎,谢毅强,等."益智-乌药"药对抗糖尿病肾病有效部位的筛选.时珍国医国药,2015;26(3)∶579~581
4徐叔云.药理实验方法学.北京:人民卫生出版社.第三版,2005∶1516
5 Schernthaner G,Schernthaner GH.Diabetic nephropathy:new approache for improving glycemic control and reducing risk.J Nephrol,2013;26(6)∶975~985
6 Wolf G,Schanze A,Stahl R,et al.P27kip1knockout mice are pro-tected from diabetic nephropathy:Evidence for p27kip1 haplotype insufficiency.Kidney Int,2005;68(4)∶1583~1589
7 Liu M1,Zhang Y,Chi Y,et al.Delivery of megsin si RNA plasmidreveal therapeutic potential against diabetic nephropathy by down-regulating p27(kip1)level.J Nephrol,2012;25(3)∶418~425
8李俊美.吕仁和教授治疗糖尿病肾病的经验.四川中医,2009;27(5)∶1~3
9赵良斌,李明权,何玉华,等.叶传蕙教授治疗糖尿病肾病用药经验.中华中医药杂志,2013;28(6)∶1764~1767
10陈萍,王培培,焦泽沼,等.益智仁的化学成分及药理活性研究进展.现代药物与临床,2013;28(4)∶617~623
11谢毅强,刘嫱,韦祎,等.益智提取物抗糖尿病肾病有效部位的筛选.中国实验方剂学杂志,2015;21(3)∶114~117
12刘嫱,房磊臣,韦祎,等.益智仁提取物对糖尿病肾病模型小鼠的疗效及机制研究.中国医药导报,2015;12(33)∶21~25
13闫利利,王金芳,李秋月,等.乌药的化学成分研究.中国药业,2013;22(2)∶16~17