一株亚硝酸盐降解菌的分离鉴定及其降解特性
|
摘要
为获得高效降解亚硝酸盐菌,从鄱阳湖水域筛选出1株新型反硝化细菌,命名为X10。本实验对菌株进行16S rDNA基因序列分析,构建系统发育树并结合生化指标对该菌株进行鉴定;研究了亚硝酸盐浓度、pH值、温度、接种量对其生长及脱氮能力的影响,以及菌株对养殖水体中亚硝酸盐氮的降解能力。结果显示,X10鉴定为木糖氧化无色杆菌;温度30°C、pH 7.0、接种量3%,经48 h培养后菌株对筛选培养基中亚硝酸盐(浓度300 mg/L)的降解率最高,达99.8%;将该菌液(2×10~8 CFU/mL)按1%的接种量加入人工养殖池塘水中(亚硝酸盐质量浓度为0.45 mg/L,pH为6.7),28°C水温下经96 h后亚硝酸盐降解率达82.6%;安全性评价实验显示,X10菌落周围无溶血现象,并且高浓度菌液(5×10~8 CFU/mL)对斑马鱼无致死作用。研究表明X10在水产养殖中具有较好的应用前景。
In this paper, a new and highly efficient denitrifying bacterium strain from Poyang Lake was screened and named X10. The physiological and biochemical characteristics, 16 S rDNA gene sequence analysis, and the effects of nitrite concentration, pH value, temperature and inoculum size on the growth and nitrogen removal ability of the strain were researched. Meanwhile, the nitrite degradation capacity of X10 in aquaculture water was also studied. The results showed that the strain X10 was identified as Acinetobacter xylosoxidans. The optimal incubation conditions for the aerobic denitrifying strain X10 were: pH, 7.0; inoculation size, 3%; temperature, 30 °C;nitrite concentration, 300 mg/L. Under this circumstance, the degradum rate of nitrite reached peak after 48 h, up to 99.8%. When X10 bacteria(2×10~8 CFU/mL) were added into cultured pond water(28°C), the degradation rate of nitrite was 82.6% after 96 h. The safety evaluation test showed that the high concentration of X10(5 × 10~8 CFU/mL) had no lethal effect on Danio rerio and no hemolysis was found. These results indicated that X10 can be applied widely in aquaculture.
In this paper, a new and highly efficient denitrifying bacterium strain from Poyang Lake was screened and named X10. The physiological and biochemical characteristics, 16 S rDNA gene sequence analysis, and the effects of nitrite concentration, pH value, temperature and inoculum size on the growth and nitrogen removal ability of the strain were researched. Meanwhile, the nitrite degradation capacity of X10 in aquaculture water was also studied. The results showed that the strain X10 was identified as Acinetobacter xylosoxidans. The optimal incubation conditions for the aerobic denitrifying strain X10 were: pH, 7.0; inoculation size, 3%; temperature, 30 °C;nitrite concentration, 300 mg/L. Under this circumstance, the degradum rate of nitrite reached peak after 48 h, up to 99.8%. When X10 bacteria(2×10~8 CFU/mL) were added into cultured pond water(28°C), the degradation rate of nitrite was 82.6% after 96 h. The safety evaluation test showed that the high concentration of X10(5 × 10~8 CFU/mL) had no lethal effect on Danio rerio and no hemolysis was found. These results indicated that X10 can be applied widely in aquaculture.
引文
[1]吴雅丽,许海,杨桂军,等.太湖水体氮素污染状况研究进展[J].湖泊科学,2014,26(1):19-28.Wu Y L,Xu H,Yang G J,et al.Progress in nitrogen pollution research in Lake Taihu[J].Journal of Lake Sciences,2014,26(1):19-28(in Chinese).
[2]吴伟,范立民.水产养殖环境的污染及其控制对策[J].中国农业科技导报,2014,16(2):26-34.Wu W,Fan L M.Pollution and control measures of aquaculture environment[J].Journal of Agricultural Science and Technology,2014,16(2):26-34(in Chinese).
[3]何腾霞,李振轮,杨珊.好氧反硝化细菌及其去除亚硝酸盐的作用机理研究进展[J].过程工程学报,2015,15(1):174-180.He T X,Li Z L,Yang S.Research progress in aerobic denitrifier and its removing mechanism of nitrite[J].The Chinese Journal of Process Engineering,2015,15(1):174-180(in Chinese).
[4]张卫强,朱英.养殖水体中氨氮的危害及其检测方法研究进展[J].环境卫生学杂志,2012,2(6):324-327.Zhang W Q,Zhu Y.Advances on the research of the hazard of ammonia nitrogen in aquaculture water and its determination method[J].Journal of Environmental Hygiene,2012,2(6):324-327(in Chinese).
[5]高明辉,马立保,葛立安,等.亚硝酸盐在水生动物体内的吸收机制及蓄积的影响因素[J].南方水产,2008,4(4):73-79.Gao M H,Ma L B,Ge L A,et al.Nitrite uptake mechanism and the influencing factors of accumulation in aquatic animals[J].South China Fisheries Science,2008,4(4):73-79(in Chinese).
[6]李继洲,程南宁,陈清锦.污染水体的生物修复技术研究进展[J].环境污染治理技术与设备,2005,6(1):25-30.Li J Z,Cheng N N,Chen Q J.Progress in the study of bioremediation for polluted water bodies[J].Techniques and Equipment for Environmental Pollution Control,2005,6(1):25-30(in Chinese).
[7]刘道玉,吴伟.水产养殖水体污染及微生物修复的研究[J].现代农业科技,2011(17):253-256.Liu D Y,Wu W.Research on water pollution and microbial remediation of aquaculture[J].Modern Agricultural Sciences and Technology,2011(17):253-256(in Chinese).
[8]刘慧玲,张红莲,李细钊.光合细菌Ⅰ的分离及其对水体中亚硝酸盐降解的研究[J].水产科学,2005,24(6):32-33.Liu H L,Zhang H L,Li X Z.Degradation of nitrite by photosynthetic bacterium-Ⅰ(PSB-Ⅰ)isolated from shrimp culture ponds[J].Fisheries Science,2005,24(6):32-33(in Chinese).
[9]Guo Y,Zhou X M,Li Y G,et al.Heterotrophic nitrification and aerobic denitrification by a novel Halomonas campisalis[J].Biotechnology Letters,2013,35(12):2045-2049.
[10]安健,宋增福,杨先乐,等.好氧反硝化细菌YX-6特性及鉴定分析[J].中国水产科学,2010,17(3):561-569.An J,Song Z F,Yang X L,et al.Characteristics of aerobic denitrifying strain YX-6 and identification[J].Journal of Fishery Sciences of China,2010,17(3):561-569(in Chinese).
[11]邓振山,张春晓.两株养殖污泥中亚硝酸盐降解菌的筛选及其降解条件[J].环境工程,2016,34(S1):176-179,234.Deng Z S,Zhang C X.Identification and removal condition of two nitrite removing bacteria from aquaculture sediment[J].Environmental Engineering,2016,34(S1):176-179,234(in Chinese).
[12]杨艳,刘萍,马鹏飞,等.巨大芽孢杆菌MPF-906对养鱼水质净化的初步研究[J].水产养殖,2007,28(3):6-8.Yang Y,Liu P,Ma P F,et al.Research on the role of B.megaterium MPF-906 in purifying water quality of fish cultivating[J].Journal of Aquaculture,2007,28(3):6-8(in Chinese).
[13]R E布坎南,N E吉本斯.伯杰细菌鉴定手册[M].第8版.中国科学院微生物研究所《伯杰细菌鉴定手册》翻译组,译.北京:科学出版社,1984:677-830.Buchanan R E,Gibbons N E.Bergey's Manual of Determinative Bacteriology[M].8th ed.Translation team of Berger Bacteria Identification Manual,Institute of Microbiology,Chinese Academy of Sciences,Trans.Beijing:Science Press,1984:677-830(in Chinese).
[14]熊焰,刘力源,罗睿,等.1株亚硝酸盐降解苗的筛选、鉴定、降解条件及效果[J].中国水产科学,2010,17(6):1264-1271.Xiong Y,Liu L Y,Luo R,et al.Screening,identification,degradation condition and effects of a nitrite-degradating strain[J].Journal of Fishery Sciences of China,2010,17(6):1264-1271(in Chinese).
[15]田雪,范鹏浩,邹鹏,等.木糖氧化无色杆菌P1对芘降解的研究及其在两相分配生物反应系统中的应用[J].北京化工大学学报(自然科学版),2016,43(5):63-68.Tian X,Fan P H,Zou P,et al.Degradation of pyrene by Achromobacter xylosoxidans P1 and its application in a two-phase partitioning bioreactor[J].Journal of Beijing University of Chemical Technology(Natural Science Edition),2016,43(5):63-68(in Chinese).
[16]司美茹,赵云峰,苏涛.高效降解烷烃的无色杆菌XL株的分离鉴定及其降解特性[J].土壤通报,2011,42(3):562-567.Si M R,Zhao Y F,Su T.Isolation and identification of a high-efficiency alkane-degrading Achromobacter insolitus XL strain and its degradation characteristics[J].Chinese Journal of Soil Science,2011,42(3):562-567(in Chinese).
[17]唐玉斌,孙常宇,陈芳艳,等.一株[艹屈]高效降解菌的分离鉴定及其降解特性[J].微生物学通报,2009,36(4):593-597.Tang Y B,Sun C Y,Chen F Y,et al.Isolation and identification of a chrysene-degrading strain and its degradation characteristics[J].Microbiology China,2009,36(4):593-597(in Chinese).
[18]李哲,陈潼樾,冷粟,等.一株氧化木糖无色杆菌对Pb的生物矿化作用及其应用效果研究[J].农业环境科学学报,2017,36(10):2014-2020.Li Z,Chen T Y,Leng S,et al.Biomineralization of Pb by a strain of Achromobacter xylosoxidans and its practical application in bioremediation[J].Journal of Agro-Environment Science,2017,36(10):2014-2020(in Chinese).
[19]武凤霞,范丙全,刘建玲.木糖氧化无色杆菌反硝化亚种细菌的分离鉴定及其菲降解特性研究[J].植物营养与肥料学报,2007,13(4):725-729.Wu F X,Fan B Q,Liu J L.Isolation of Achromobacter xylosoxidans subsp.Denitrificans and it’s degradation of phenanthrene[J].Plant Nutrition and Fertilizer Science,2007,13(4):725-729(in Chinese).
[20]Zhu L,Ding W,Feng L J,et al.Isolation of aerobic denitrifiers and characterization for their potential application in the bioremediation of oligotrophic ecosystem[J].Bioresource Technology,2012,108:1-7.
[21]Chen Q,Ni J R.Heterotrophic nitrification-aerobic denitrification by novel isolated bacteria[J].Journal of Industrial Microbiology&Biotechnology,2011,38(9):1305-1310.
[22]姚秀清,刘秀梅.反硝化细菌降解养殖水体亚硝酸盐的研究[J].应用化工,2014,43(5):871-873.Yao X Q,Liu X M.The research of degradation of nitrite by denitrifying bacteria in the aquaculture water[J].Applied Chemical Industry,2014,43(5):871-873(in Chinese).
[23]杨浩锋,唐佳玙,胡安辉,等.一株反硝化细菌的分离鉴定及其反硝化特性[J].环境工程学报,2014,8(1):366-371.Yang H F,Tang J Y,Hu A H,et al.Identification and denitrification characteristics of a denitrifier[J].Chinese Journal of Environmental Engineering,2014,8(1):366-371(in Chinese).
[24]?imek M,Jí?ováL,Hopkins D W.What is the so-called optimum pH for denitrification in soil?[J].Soil Biology and Biochemistry,2002,34(9):1227-1234.
[25]王燕,王淑莹,孙洪伟,等.pH和MLSS对短程反硝化过程NO2-还原速率的影响[J].中国给水排水,2010,26(13):25-28.Wang Y,Wang S Y,Sun H W,et al.Effects of pH and MLSS on nitrite reduction rate in shortcut denitrification[J].China Water&Wastewater,2010,26(13):25-28(in Chinese).
[26]李卫芬,郑佳佳,张小平,等.反硝化酶及其环境影响因子的研究进展[J].水生生物学报,2014,38(1):166-170.Li W F,Zheng J J,Zhang X P,et al.Progress in studies on denitrification enzymes and environmental impact factors[J].Acta Hydrobiologica Sinica,2014,38(1):166-170(in Chinese).
[27]Carrera J,Vicent T,Lafuente F J.Influence of temperature on denitrification of an industrial high-strength nitrogen wastewater in a two-sludge system[J].Water SA,2004,29(1):11-16.
[28]李焱生,魏民,张艾晓,等.一株异养型亚硝酸盐氧化细菌的分离及其降解特性的研究[J].生物技术通报,2010(5):196-202.Li Y S,Wei M,Zhang A X,et al.Isolation,identification and characterization of a nitrite-oxidizing bacterium[J].Biotechnology Bulletin,2010(5):196-202(in Chinese).
[29]杨小龙,李文明,陈燕,等.一株好氧反硝化菌的分离鉴定及其除氮特性[J].微生物学报,2011,51(8):1062-1070.Yang X L,Li W M,Chen Y,et al.Identification and denitrification of an aerobic bacterium[J].Acta Microbiologica Sinica,2011,51(8):1062-1070(in Chinese).
[30]陈薇,丁祥力,贺月林,等.亚硝酸盐降解菌的分离鉴定及其降解特性[J].环境科学与技术,2011,34(S2):37-41.Chen W,Ding X L,He Y L,et al.Isolation,identification and degradation characteristics of nitrite degradation bactercia[J].Environmental Science&Technology,2011,34(S2):37-41(in Chinese).
[31]邓爱娟,刘敏,刘志雄,等.洪湖地区养殖鱼塘春夏季水温变化及预报研究[J].中国农学通报,2013,29(29):61-68.Deng A M,Liu M,Liu Z X,et al.Water temperature change and forecast in spring and summer in aquaculture fish pond’s in Honghu Area[J].Chinese Agricultural Science Bulletin,2013,29(29):61-68(in Chinese).
[32]孟翠丽,杨文刚,干昌林,等.淡水鱼塘分层水温的变化特征[J].中国农学通报,2015,31(11):103-108.Meng C L,Yang W G,Gan C L,et al.Variation characteristics of water temperatures at different depths of fishpond[J].Chinese Agricultural Science Bulletin,2015,31(11):103-108(in Chinese).
[33]何腾霞,李振轮,徐义.耐冷亚硝酸盐型反硝化细菌Pseudomonas putida Y-12脱氮特性[J].环境科学学报,2015,35(10):3071-3077.He T X,Li Z L,Xu Y.Denitrification characteristics of a hypothermia nitrite-denitrifier Pseudomonas putida Y12 [J].Acta Scientiae Circumstantiae,2015,35(10):3071-3077(in Chinese).
[34]何伟,王薇,王洁,等.一株好氧反硝化菌的分离鉴定及其混合应用特性研究[J].生态与农村环境学报,2009,25(2):88-93.He W,Wang W,Wang J,et al.Isolation,identification and its mixed application of a strain of aerobic denitrifying bacteria[J].Journal of Ecology and Rural Environment,2009,25(2):88-93(in Chinese).
[2]吴伟,范立民.水产养殖环境的污染及其控制对策[J].中国农业科技导报,2014,16(2):26-34.Wu W,Fan L M.Pollution and control measures of aquaculture environment[J].Journal of Agricultural Science and Technology,2014,16(2):26-34(in Chinese).
[3]何腾霞,李振轮,杨珊.好氧反硝化细菌及其去除亚硝酸盐的作用机理研究进展[J].过程工程学报,2015,15(1):174-180.He T X,Li Z L,Yang S.Research progress in aerobic denitrifier and its removing mechanism of nitrite[J].The Chinese Journal of Process Engineering,2015,15(1):174-180(in Chinese).
[4]张卫强,朱英.养殖水体中氨氮的危害及其检测方法研究进展[J].环境卫生学杂志,2012,2(6):324-327.Zhang W Q,Zhu Y.Advances on the research of the hazard of ammonia nitrogen in aquaculture water and its determination method[J].Journal of Environmental Hygiene,2012,2(6):324-327(in Chinese).
[5]高明辉,马立保,葛立安,等.亚硝酸盐在水生动物体内的吸收机制及蓄积的影响因素[J].南方水产,2008,4(4):73-79.Gao M H,Ma L B,Ge L A,et al.Nitrite uptake mechanism and the influencing factors of accumulation in aquatic animals[J].South China Fisheries Science,2008,4(4):73-79(in Chinese).
[6]李继洲,程南宁,陈清锦.污染水体的生物修复技术研究进展[J].环境污染治理技术与设备,2005,6(1):25-30.Li J Z,Cheng N N,Chen Q J.Progress in the study of bioremediation for polluted water bodies[J].Techniques and Equipment for Environmental Pollution Control,2005,6(1):25-30(in Chinese).
[7]刘道玉,吴伟.水产养殖水体污染及微生物修复的研究[J].现代农业科技,2011(17):253-256.Liu D Y,Wu W.Research on water pollution and microbial remediation of aquaculture[J].Modern Agricultural Sciences and Technology,2011(17):253-256(in Chinese).
[8]刘慧玲,张红莲,李细钊.光合细菌Ⅰ的分离及其对水体中亚硝酸盐降解的研究[J].水产科学,2005,24(6):32-33.Liu H L,Zhang H L,Li X Z.Degradation of nitrite by photosynthetic bacterium-Ⅰ(PSB-Ⅰ)isolated from shrimp culture ponds[J].Fisheries Science,2005,24(6):32-33(in Chinese).
[9]Guo Y,Zhou X M,Li Y G,et al.Heterotrophic nitrification and aerobic denitrification by a novel Halomonas campisalis[J].Biotechnology Letters,2013,35(12):2045-2049.
[10]安健,宋增福,杨先乐,等.好氧反硝化细菌YX-6特性及鉴定分析[J].中国水产科学,2010,17(3):561-569.An J,Song Z F,Yang X L,et al.Characteristics of aerobic denitrifying strain YX-6 and identification[J].Journal of Fishery Sciences of China,2010,17(3):561-569(in Chinese).
[11]邓振山,张春晓.两株养殖污泥中亚硝酸盐降解菌的筛选及其降解条件[J].环境工程,2016,34(S1):176-179,234.Deng Z S,Zhang C X.Identification and removal condition of two nitrite removing bacteria from aquaculture sediment[J].Environmental Engineering,2016,34(S1):176-179,234(in Chinese).
[12]杨艳,刘萍,马鹏飞,等.巨大芽孢杆菌MPF-906对养鱼水质净化的初步研究[J].水产养殖,2007,28(3):6-8.Yang Y,Liu P,Ma P F,et al.Research on the role of B.megaterium MPF-906 in purifying water quality of fish cultivating[J].Journal of Aquaculture,2007,28(3):6-8(in Chinese).
[13]R E布坎南,N E吉本斯.伯杰细菌鉴定手册[M].第8版.中国科学院微生物研究所《伯杰细菌鉴定手册》翻译组,译.北京:科学出版社,1984:677-830.Buchanan R E,Gibbons N E.Bergey's Manual of Determinative Bacteriology[M].8th ed.Translation team of Berger Bacteria Identification Manual,Institute of Microbiology,Chinese Academy of Sciences,Trans.Beijing:Science Press,1984:677-830(in Chinese).
[14]熊焰,刘力源,罗睿,等.1株亚硝酸盐降解苗的筛选、鉴定、降解条件及效果[J].中国水产科学,2010,17(6):1264-1271.Xiong Y,Liu L Y,Luo R,et al.Screening,identification,degradation condition and effects of a nitrite-degradating strain[J].Journal of Fishery Sciences of China,2010,17(6):1264-1271(in Chinese).
[15]田雪,范鹏浩,邹鹏,等.木糖氧化无色杆菌P1对芘降解的研究及其在两相分配生物反应系统中的应用[J].北京化工大学学报(自然科学版),2016,43(5):63-68.Tian X,Fan P H,Zou P,et al.Degradation of pyrene by Achromobacter xylosoxidans P1 and its application in a two-phase partitioning bioreactor[J].Journal of Beijing University of Chemical Technology(Natural Science Edition),2016,43(5):63-68(in Chinese).
[16]司美茹,赵云峰,苏涛.高效降解烷烃的无色杆菌XL株的分离鉴定及其降解特性[J].土壤通报,2011,42(3):562-567.Si M R,Zhao Y F,Su T.Isolation and identification of a high-efficiency alkane-degrading Achromobacter insolitus XL strain and its degradation characteristics[J].Chinese Journal of Soil Science,2011,42(3):562-567(in Chinese).
[17]唐玉斌,孙常宇,陈芳艳,等.一株[艹屈]高效降解菌的分离鉴定及其降解特性[J].微生物学通报,2009,36(4):593-597.Tang Y B,Sun C Y,Chen F Y,et al.Isolation and identification of a chrysene-degrading strain and its degradation characteristics[J].Microbiology China,2009,36(4):593-597(in Chinese).
[18]李哲,陈潼樾,冷粟,等.一株氧化木糖无色杆菌对Pb的生物矿化作用及其应用效果研究[J].农业环境科学学报,2017,36(10):2014-2020.Li Z,Chen T Y,Leng S,et al.Biomineralization of Pb by a strain of Achromobacter xylosoxidans and its practical application in bioremediation[J].Journal of Agro-Environment Science,2017,36(10):2014-2020(in Chinese).
[19]武凤霞,范丙全,刘建玲.木糖氧化无色杆菌反硝化亚种细菌的分离鉴定及其菲降解特性研究[J].植物营养与肥料学报,2007,13(4):725-729.Wu F X,Fan B Q,Liu J L.Isolation of Achromobacter xylosoxidans subsp.Denitrificans and it’s degradation of phenanthrene[J].Plant Nutrition and Fertilizer Science,2007,13(4):725-729(in Chinese).
[20]Zhu L,Ding W,Feng L J,et al.Isolation of aerobic denitrifiers and characterization for their potential application in the bioremediation of oligotrophic ecosystem[J].Bioresource Technology,2012,108:1-7.
[21]Chen Q,Ni J R.Heterotrophic nitrification-aerobic denitrification by novel isolated bacteria[J].Journal of Industrial Microbiology&Biotechnology,2011,38(9):1305-1310.
[22]姚秀清,刘秀梅.反硝化细菌降解养殖水体亚硝酸盐的研究[J].应用化工,2014,43(5):871-873.Yao X Q,Liu X M.The research of degradation of nitrite by denitrifying bacteria in the aquaculture water[J].Applied Chemical Industry,2014,43(5):871-873(in Chinese).
[23]杨浩锋,唐佳玙,胡安辉,等.一株反硝化细菌的分离鉴定及其反硝化特性[J].环境工程学报,2014,8(1):366-371.Yang H F,Tang J Y,Hu A H,et al.Identification and denitrification characteristics of a denitrifier[J].Chinese Journal of Environmental Engineering,2014,8(1):366-371(in Chinese).
[24]?imek M,Jí?ováL,Hopkins D W.What is the so-called optimum pH for denitrification in soil?[J].Soil Biology and Biochemistry,2002,34(9):1227-1234.
[25]王燕,王淑莹,孙洪伟,等.pH和MLSS对短程反硝化过程NO2-还原速率的影响[J].中国给水排水,2010,26(13):25-28.Wang Y,Wang S Y,Sun H W,et al.Effects of pH and MLSS on nitrite reduction rate in shortcut denitrification[J].China Water&Wastewater,2010,26(13):25-28(in Chinese).
[26]李卫芬,郑佳佳,张小平,等.反硝化酶及其环境影响因子的研究进展[J].水生生物学报,2014,38(1):166-170.Li W F,Zheng J J,Zhang X P,et al.Progress in studies on denitrification enzymes and environmental impact factors[J].Acta Hydrobiologica Sinica,2014,38(1):166-170(in Chinese).
[27]Carrera J,Vicent T,Lafuente F J.Influence of temperature on denitrification of an industrial high-strength nitrogen wastewater in a two-sludge system[J].Water SA,2004,29(1):11-16.
[28]李焱生,魏民,张艾晓,等.一株异养型亚硝酸盐氧化细菌的分离及其降解特性的研究[J].生物技术通报,2010(5):196-202.Li Y S,Wei M,Zhang A X,et al.Isolation,identification and characterization of a nitrite-oxidizing bacterium[J].Biotechnology Bulletin,2010(5):196-202(in Chinese).
[29]杨小龙,李文明,陈燕,等.一株好氧反硝化菌的分离鉴定及其除氮特性[J].微生物学报,2011,51(8):1062-1070.Yang X L,Li W M,Chen Y,et al.Identification and denitrification of an aerobic bacterium[J].Acta Microbiologica Sinica,2011,51(8):1062-1070(in Chinese).
[30]陈薇,丁祥力,贺月林,等.亚硝酸盐降解菌的分离鉴定及其降解特性[J].环境科学与技术,2011,34(S2):37-41.Chen W,Ding X L,He Y L,et al.Isolation,identification and degradation characteristics of nitrite degradation bactercia[J].Environmental Science&Technology,2011,34(S2):37-41(in Chinese).
[31]邓爱娟,刘敏,刘志雄,等.洪湖地区养殖鱼塘春夏季水温变化及预报研究[J].中国农学通报,2013,29(29):61-68.Deng A M,Liu M,Liu Z X,et al.Water temperature change and forecast in spring and summer in aquaculture fish pond’s in Honghu Area[J].Chinese Agricultural Science Bulletin,2013,29(29):61-68(in Chinese).
[32]孟翠丽,杨文刚,干昌林,等.淡水鱼塘分层水温的变化特征[J].中国农学通报,2015,31(11):103-108.Meng C L,Yang W G,Gan C L,et al.Variation characteristics of water temperatures at different depths of fishpond[J].Chinese Agricultural Science Bulletin,2015,31(11):103-108(in Chinese).
[33]何腾霞,李振轮,徐义.耐冷亚硝酸盐型反硝化细菌Pseudomonas putida Y-12脱氮特性[J].环境科学学报,2015,35(10):3071-3077.He T X,Li Z L,Xu Y.Denitrification characteristics of a hypothermia nitrite-denitrifier Pseudomonas putida Y12 [J].Acta Scientiae Circumstantiae,2015,35(10):3071-3077(in Chinese).
[34]何伟,王薇,王洁,等.一株好氧反硝化菌的分离鉴定及其混合应用特性研究[J].生态与农村环境学报,2009,25(2):88-93.He W,Wang W,Wang J,et al.Isolation,identification and its mixed application of a strain of aerobic denitrifying bacteria[J].Journal of Ecology and Rural Environment,2009,25(2):88-93(in Chinese).