盐度和干露对大竹蛏呼吸代谢相关酶的影响
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摘要
为探讨盐度和干露两种胁迫对大竹蛏呼吸代谢酶活力的影响,在实验室条件下分别研究盐度胁迫(15、20、25、30、35)和干露胁迫(6、12、24、48h)条件下,大竹蛏乳酸脱氢酶、碱性磷酸酶和琥珀酸脱氢酶3种酶活力的变化规律。结果显示,当盐度逐渐升高时,碱性磷酸酶的活性呈降低趋势,当盐度为20时,酶活性最低,为0.043U/g,当盐度为25时,酶活性最高,为0.053U/g。盐度对琥珀酸脱氢酶和乳酸脱氢酶呈现波纹型的影响,在盐度为25时,琥珀酸脱氢酶活性较高,为0.401U/mg;盐度为25时,乳酸脱氢酶活性较高,为0.604U/g,盐度为20时,乳酸脱氢酶活性显著降低,为0.287U/g,盐度为35时,乳酸脱氢酶活性无显著变化。当干露时间逐渐延长,从整体变化情况来看,琥珀酸脱氢酶、碱性磷酸酶和乳酸脱氢酶这3种酶的活性逐渐降低,当干露时间达到48h,3种酶活性均达到最低,分别为0.197U/mg、0.020U/g和0.187U/g。说明盐度和干露胁迫对大竹蛏呼吸代谢酶活力有显著影响(P<0.05)。研究结果可为大竹蛏的人工养殖和运输提供参考。
The activities of metabolic enzymes in respiratory including alkaline phosphatase(ALP),succinate dehydrogenase(SDH),and lactate dehydrogenase(LDH)were determined in grand jackknife clam Solen grandis with shell length of(9.16±0.5)cm exposed to a salinity of 10,15,20,25 and 30and to air for 6,12,24,and 48 hat water temperature of(14±1)℃to investigate the effects of different salinities and desiccation on activities of metabolic enzymes in respiratory in grand jackknife clam.The activity of ALP was shown to be decreased with the increase in salinity,with the minimum(0.043U/g)at a salinity of 20,and the maximum(0.053U/g)at a salinity of 25.The high activity of SDH was found at a salinity of 25(0.401U/mg),and high activity of LDH a salinity of 25(0.604U/g),decline at a salinity of 20(0.287U/g).The activities of SDH,ALP and LDH were shown to be decreased with the prolonged air exposure,with the minimal value of 0.197U/mg for SDH,0.020U/g for ALP and 0.187U/g for LDH at 48 h,indicating that high salinity and long period of air exposure influence activities of metabolic enzymes significantly(P<0.05).The findings provide references with grand jackknife clam culture and transportation.
The activities of metabolic enzymes in respiratory including alkaline phosphatase(ALP),succinate dehydrogenase(SDH),and lactate dehydrogenase(LDH)were determined in grand jackknife clam Solen grandis with shell length of(9.16±0.5)cm exposed to a salinity of 10,15,20,25 and 30and to air for 6,12,24,and 48 hat water temperature of(14±1)℃to investigate the effects of different salinities and desiccation on activities of metabolic enzymes in respiratory in grand jackknife clam.The activity of ALP was shown to be decreased with the increase in salinity,with the minimum(0.043U/g)at a salinity of 20,and the maximum(0.053U/g)at a salinity of 25.The high activity of SDH was found at a salinity of 25(0.401U/mg),and high activity of LDH a salinity of 25(0.604U/g),decline at a salinity of 20(0.287U/g).The activities of SDH,ALP and LDH were shown to be decreased with the prolonged air exposure,with the minimal value of 0.197U/mg for SDH,0.020U/g for ALP and 0.187U/g for LDH at 48 h,indicating that high salinity and long period of air exposure influence activities of metabolic enzymes significantly(P<0.05).The findings provide references with grand jackknife clam culture and transportation.
引文
[1]吴杨平,陈爱华,姚国兴,等.大竹蛏生物学特性的初步研究[J].水产科学,2011,30(12):764-767.
[2]吴杨平,陈爱华,姚国兴,等.大竹蛏稚贝滤水率的研究[J].海洋科学,2011,35(1):6-9.
[3]阎希柱,王桂忠,李少菁.盐度对九孔鲍能量收支的影响[J].生态学杂志,2009,28(8):1520-1524.
[4]聂鸿涛,姜力文,王海伦,等.不同温度和盐度对大竹蛏耗氧率和排氨率的影响[J].海洋科学,2017,41(4):44-50.
[5]高杉,周遵春,董颖,等.盐度及pH突变对大竹蛏稚贝抗氧化酶活力的影响[J].大连海洋大学学报,2017,32(1):62-67.
[6]陈爱华,姚国兴,张志伟,等.温度,盐度和底质对大竹蛏稚贝生长及存活的影响[J].热带海洋学报,2010,29(5):94-97.
[7]张鹭,陈寅山,彭宣宪.低盐压力下缢蛏血淋巴蛋白的比较蛋白质组学研究[J].吉林农业大学学报,2004,26(3):272-274.
[8]于瑞海,王昭萍,孔令锋,等.不同发育期的太平洋牡蛎在不同干露状态下的成活率研究[J].中国海洋大学学报:自然科学版,2006,36(4):617-620.
[9]尤仲杰,陆彤霞,马斌,等.几种环境因子对墨西哥湾扇贝幼虫和稚贝生长与存活的影响[J].热带海洋学报,2003,22(3):22-29.
[10]曹琛,匡少华,姜超,等.干露及盐度变化对毛蚶苗的影响试验[J].河北渔业,2007(5):38-38.
[11]杨凤,谭文明,闫喜武,等.干露及淡水浸泡对菲律宾蛤仔稚贝生长和存活的影响[J].水产科学,2012,31(3):143-146.
[12]林小涛,张秋明,许忠能,等.虾蟹类呼吸代谢研究进展[J].水产学报,2000,24(6):575-580.
[13]路允良,王芳,高勤峰,等.盐度对三疣梭子蟹成熟前后呼吸代谢的影响[J].水产学报,2012,36(9):1392-1399.
[14]管越强,李利,王慧春,等.低氧胁迫对日本沼虾呼吸代谢和抗氧化能力的影响[J].河北大学学报:自然科学版,2010,30(3):301-306.
[15]Kaja S,Payne A J,Singh T,et al.An optimized lactate dehydrogenase release assay for screening of drug candidates in neuroscience[J].Journal of Pharmacological and Toxicological Methods,2015(73):1-6.
[16]Tian J,Jia H,Yu J.A description of alkaline phosphatases from marine organisms[J].Chinese Journal of Oceanology and Limnology,2016,34(4):795-809.
[17]Tettelbach S T,Rhodes E W.Combined effects of temperature and salinity on embryos and larvae of northern bay scallop,Argopecten irradians[J].Marine Biology,1981,63(3):249-256.
[18]Castagna M.Culture of the bay scallop,Argopecten irradians in Virginia[J].Marine Fisheries Review,1975(37):19-24.
[19]鹿瑶,刘辉,聂鸿涛,等.辽宁沿海薄片镜蛤的繁殖周期研究[J].大连海洋大学学报,2015,30(6):647-652.
[20]张志伟,姚国兴,陈爱华.大竹蛏同工酶组织特异性与多态性初步研究[J].海洋科学,2009,33(3):41-43.
[21]张滔,刘相全,孙振兴,等.大竹蛏(Solen grandis)不同地理群体遗传多样性的AFLP分析[J].海洋与湖沼,2013,44(2):525-530.
[22]张志伟,陈爱华,张志勇,等.大竹蛏5个野生群体遗传多样性的微卫星分析[J].中国水产科学,2015,22(4):740-748.
[23]宋贤亭,于瑞海,马培振,等.大竹蛏室内人工育苗技术研究[J].海洋湖沼通报,2015(4):56-60.
[24]Viruu M.Difference in effects of various training regimens on metabolism of skeletal muscles[J].Journal of Sports Medicine and Physical Fitness,1994,34(3):217-227.
[25]蔡彬,江幸福,罗礼智,等.温、湿度对粘虫蛾飞行能源物质利用的影响[J].生态学报,2002,22(7):1068-1074.
[26]Zietara M S,Gronczewska J,Stachowiak K,et al.Lactate dehydrogenase in abdominal muscle of crayfish Orconects limosus and shrimp Crangon crangon(Decapoda:Crustacea):properities and evolutionary relationship[J].Comparative Biochemistry and Physiology—Part B:Biochemistry&Molecular Biology,1996,114(4):395-401.
[27]周永灿,潘金培.贝类细胞和体液的防御机制研究进展[J].水产学报,1997,21(4):449-454.
[28]王瑞芳,庄平,冯广朋,等.盐度升高对中华绒螯蟹几种非特异性免疫因子的影响[J].水产学报,2012,36(4):546-552.
[29]王帅,高如承,温扬敏,等.盐度突变对中国血蛤非特异性免疫酶活性的影响[J].江苏农业科学,2008(5):213-215.
[30]李畅,侯玉霞,覃兆海.琥珀酸脱氢酶的结构与活性氧的产生[J].世界农药,2009,31(2):17-21.
[31]Zhang R Q,Chen Q X,Zheng W Z,et al.Inhibition kinetics of green crab(Scylla serrata)alkaline phosphatase activity by dithiothreitol or 2-mercaptoethanol[J].The International Journal of Biochemistry&Cell Biology,2000,32(8):865-872.
[32]段亚飞,董宏标,王芸,等.干露胁迫对日本囊对虾呼吸代谢酶活性和RNA/DNA比值的影响[J].海洋渔业,2016,38(1):42-50.
[2]吴杨平,陈爱华,姚国兴,等.大竹蛏稚贝滤水率的研究[J].海洋科学,2011,35(1):6-9.
[3]阎希柱,王桂忠,李少菁.盐度对九孔鲍能量收支的影响[J].生态学杂志,2009,28(8):1520-1524.
[4]聂鸿涛,姜力文,王海伦,等.不同温度和盐度对大竹蛏耗氧率和排氨率的影响[J].海洋科学,2017,41(4):44-50.
[5]高杉,周遵春,董颖,等.盐度及pH突变对大竹蛏稚贝抗氧化酶活力的影响[J].大连海洋大学学报,2017,32(1):62-67.
[6]陈爱华,姚国兴,张志伟,等.温度,盐度和底质对大竹蛏稚贝生长及存活的影响[J].热带海洋学报,2010,29(5):94-97.
[7]张鹭,陈寅山,彭宣宪.低盐压力下缢蛏血淋巴蛋白的比较蛋白质组学研究[J].吉林农业大学学报,2004,26(3):272-274.
[8]于瑞海,王昭萍,孔令锋,等.不同发育期的太平洋牡蛎在不同干露状态下的成活率研究[J].中国海洋大学学报:自然科学版,2006,36(4):617-620.
[9]尤仲杰,陆彤霞,马斌,等.几种环境因子对墨西哥湾扇贝幼虫和稚贝生长与存活的影响[J].热带海洋学报,2003,22(3):22-29.
[10]曹琛,匡少华,姜超,等.干露及盐度变化对毛蚶苗的影响试验[J].河北渔业,2007(5):38-38.
[11]杨凤,谭文明,闫喜武,等.干露及淡水浸泡对菲律宾蛤仔稚贝生长和存活的影响[J].水产科学,2012,31(3):143-146.
[12]林小涛,张秋明,许忠能,等.虾蟹类呼吸代谢研究进展[J].水产学报,2000,24(6):575-580.
[13]路允良,王芳,高勤峰,等.盐度对三疣梭子蟹成熟前后呼吸代谢的影响[J].水产学报,2012,36(9):1392-1399.
[14]管越强,李利,王慧春,等.低氧胁迫对日本沼虾呼吸代谢和抗氧化能力的影响[J].河北大学学报:自然科学版,2010,30(3):301-306.
[15]Kaja S,Payne A J,Singh T,et al.An optimized lactate dehydrogenase release assay for screening of drug candidates in neuroscience[J].Journal of Pharmacological and Toxicological Methods,2015(73):1-6.
[16]Tian J,Jia H,Yu J.A description of alkaline phosphatases from marine organisms[J].Chinese Journal of Oceanology and Limnology,2016,34(4):795-809.
[17]Tettelbach S T,Rhodes E W.Combined effects of temperature and salinity on embryos and larvae of northern bay scallop,Argopecten irradians[J].Marine Biology,1981,63(3):249-256.
[18]Castagna M.Culture of the bay scallop,Argopecten irradians in Virginia[J].Marine Fisheries Review,1975(37):19-24.
[19]鹿瑶,刘辉,聂鸿涛,等.辽宁沿海薄片镜蛤的繁殖周期研究[J].大连海洋大学学报,2015,30(6):647-652.
[20]张志伟,姚国兴,陈爱华.大竹蛏同工酶组织特异性与多态性初步研究[J].海洋科学,2009,33(3):41-43.
[21]张滔,刘相全,孙振兴,等.大竹蛏(Solen grandis)不同地理群体遗传多样性的AFLP分析[J].海洋与湖沼,2013,44(2):525-530.
[22]张志伟,陈爱华,张志勇,等.大竹蛏5个野生群体遗传多样性的微卫星分析[J].中国水产科学,2015,22(4):740-748.
[23]宋贤亭,于瑞海,马培振,等.大竹蛏室内人工育苗技术研究[J].海洋湖沼通报,2015(4):56-60.
[24]Viruu M.Difference in effects of various training regimens on metabolism of skeletal muscles[J].Journal of Sports Medicine and Physical Fitness,1994,34(3):217-227.
[25]蔡彬,江幸福,罗礼智,等.温、湿度对粘虫蛾飞行能源物质利用的影响[J].生态学报,2002,22(7):1068-1074.
[26]Zietara M S,Gronczewska J,Stachowiak K,et al.Lactate dehydrogenase in abdominal muscle of crayfish Orconects limosus and shrimp Crangon crangon(Decapoda:Crustacea):properities and evolutionary relationship[J].Comparative Biochemistry and Physiology—Part B:Biochemistry&Molecular Biology,1996,114(4):395-401.
[27]周永灿,潘金培.贝类细胞和体液的防御机制研究进展[J].水产学报,1997,21(4):449-454.
[28]王瑞芳,庄平,冯广朋,等.盐度升高对中华绒螯蟹几种非特异性免疫因子的影响[J].水产学报,2012,36(4):546-552.
[29]王帅,高如承,温扬敏,等.盐度突变对中国血蛤非特异性免疫酶活性的影响[J].江苏农业科学,2008(5):213-215.
[30]李畅,侯玉霞,覃兆海.琥珀酸脱氢酶的结构与活性氧的产生[J].世界农药,2009,31(2):17-21.
[31]Zhang R Q,Chen Q X,Zheng W Z,et al.Inhibition kinetics of green crab(Scylla serrata)alkaline phosphatase activity by dithiothreitol or 2-mercaptoethanol[J].The International Journal of Biochemistry&Cell Biology,2000,32(8):865-872.
[32]段亚飞,董宏标,王芸,等.干露胁迫对日本囊对虾呼吸代谢酶活性和RNA/DNA比值的影响[J].海洋渔业,2016,38(1):42-50.