基于代谢组学的针灸作用机制研究进展
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摘要
代谢组学是新近兴起的一种系统生物学研究手段,具有整体性、动态性反应生物体接受刺激时所作出的代谢物层面的变化,与中医整体观十分吻合,因此广泛用于中医中药学的研究中,在针灸学机制的研究中早已将代谢组学作为一种有效的研究手段,近年来针灸代谢组学的研究日趋增多,在揭示针灸作用机制方面也取得了一定的成果,故对现今的研究做一综述,以愿能为代谢组学广泛应用于针灸的现代化研究提供新思路。
Metabolomics is a kind of newly emerged systematic biology research method,with integrity and dynamic nature. It can reflect the changes of metabolins when stimulating the living organism. Due to its accordance with the holism in traditional Chinese medicine( TCM),it is widely used in TCM and Chinese materia medica. In the research of acupuncture mechanism,metabonomics has been taken as an effective method of research for a long time. There are more researches of metabonomics on acupuncture and moxibustion in recent years with certain achievements in terms of revealing the mechanisms of acupuncture and moxibustion. This paper made a review on current studies,wishing to provide new ideas for metabonomics being widely used in modern acupuncture research.
Metabolomics is a kind of newly emerged systematic biology research method,with integrity and dynamic nature. It can reflect the changes of metabolins when stimulating the living organism. Due to its accordance with the holism in traditional Chinese medicine( TCM),it is widely used in TCM and Chinese materia medica. In the research of acupuncture mechanism,metabonomics has been taken as an effective method of research for a long time. There are more researches of metabonomics on acupuncture and moxibustion in recent years with certain achievements in terms of revealing the mechanisms of acupuncture and moxibustion. This paper made a review on current studies,wishing to provide new ideas for metabonomics being widely used in modern acupuncture research.
引文
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[8]Zhang A,Sun H,Qiu S,et al.Metabolomics insights into pathophysiological mechanisms of nephrology[J].Int Urol Nephrol,2014,46(5):1025-1030.
[9]Ma H,Liu X,Wu Y,et al.The Intervention Effects of Acupuncture on Fatigue Induced by Exhaustive Physical Exercises:A Metabolomics Investigation[J].Evid Based Complement Alternat Med,2015,2015:508302.
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[14]李姝婧.隔药灸脐法与针刺三阴交治疗原发性痛经的代谢组学对比研究[D].济南:山东中医药大学,2013.
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[16]韩媛媛,马玉侠,王晓翠.隔药灸脐法治疗脾虚型肠易激综合征的尿液代谢组学机制[J].广州中医药大学学报,2014,31(4):541-544.
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[23]Gao Z,Liu X,Yu S,et al.Electroacupuncture at Acupoints Reverses Plasma Glutamate,Lipid,and LDL/VLDL in an Acute Migraine Rat Model:A(1)H NMR-Based Metabolomic Study[J].Evid Based Complement Alternat Med,2014,2014:659268.
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[26]Boonstra E,de Kleijn R,Colzato LS,et al.Neurotransmitters as food supplements:the effects of GABA on brain and behavior[J].Front Psychol,2015,6:1520.
[27]Pamenter ME,Hogg DW,Ormond J,et al.Endogenous GABA(A)and GABA(B)receptor-mediated electrical suppression is critical to neuronal anoxia tolerance[J].Proc Natl Acad Sci U S A,2011,108(27):11274-11279.
[28]Gilhotra N,Dhingra D.Thymoquinone produced antianxiety-like effects in mice through modulation of GABA and NO levels[J].Pharmacol Rep,2011,63(3):660-669.
[29]Peitzsch M,Prejbisz A,KroiβM,et al.Analysis of plasma 3-methoxytyramine,normetanephrine and metanephrine by ultraperformance liquid chromatography-tandem mass spectrometry:utility for diagnosis of dopamine-producing metastatic phaeochromocytoma[J].Ann Clin Biochem,2013,50(Pt 2):147-155.
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[33]Park JM,Choi SU.Identification of a novel unpaired histidine sensor kinase affecting secondary metabolism and morphological differentiation in Streptomyces acidiscabies ATCC 49003[J].Folia Microbiol(Praha),2015,60(4):279-287.
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[35]Zhao C,Du H,Xu L,et al.Metabolomic analysis revealed glycylglycine accumulation in astrocytes after methionine enkephalin administration exhibiting neuron protective effects[J].J Pharm Biomed A-nal,2015,115:48-54.
[36]de Sousa RT,Streck EL,Forlenza OV,et al.Regulation of leukocyte tricarboxylic acid cycle in drug-na?ve Bipolar Disorder[J].Neurosci Lett,2015,605:65-68.
[37]Dong Q,Zhang X,Yu Z,et al.Synthesis reduction of central neurotransmitter 5-hydroxytryptamine by branched chain amino acid and associated antagonists improves postoperative fatigue syndrome[J].Chinese Journal of Gastrointestinal Surgery,2014,17(10):985-989.
[38]Ruan Z,Yang Y,Zhou Y,et al.Metabolomic analysis of amino acid and energy metabolism in rats supplemented with chlorogenic acid[J].Amino Acids,2014,46(9):2219-2229.
[39]Zhang A,Sun H,Wang Z,et al.Metabolomics:towards understanding traditional Chinese medicine[J].Planta Med,2010,76(17):2026-2035.
[40]Liang F,Koya D.Acupuncture:is it effective for treatment of insulin resistance?[J].Diabetes Obes Metab,2010,12(7):555-569.
[41]Wu Q,Zhang Q,Sun B,et al.1H NMR-based metabonomic study on the metabolic changes in the plasma of patients with functional dyspepsia and the effect of acupuncture[J].J Pharm Biomed Anal,2010,51(3):698-704.
[42]Wu QF,Mao S,Cai W,et al.Effects of electroacupuncture of“Weishu”(BL 21)and“Zhongwan”(CV 12)on serum large molecular metabolites in functional dyspepsia rats[J].Acupuncture Research,2010,35(4):287-292.
[43]陈大帅.针刺天枢穴治疗功能性便秘的~1H NMR代谢组学研究[D].成都:成都中医药大学,2014.
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[45]Lee CC,Kasa-Vubu JZ,Supiano MA.Differential effects of raloxifene and estrogen on insulin sensitivity in postmenopausal women[J].JAm Geriatr Soc,2003,51(5):683-688.
[46]Yeh PS,Lin HJ,Chen PS,et al.Effect of statin treatment on threemonth outcomes in patients with stroke-associated infection:a prospective cohort study[J].Eur J Neurol,2012,19(5):689-695.
[47]Munshi SU,Taneja S,Bhavesh NS,et al.Metabonomic analysis of hepatitis E patients shows deregulated metabolic cycles and abnormalities in amino acid metabolism[J].J Viral Hepat,2011,18(10):e591-602.
[48]Ruan Z,Yang Y,Wen Y,et al.Metabolomic analysis of amino acid and fat metabolism in rats with L-tryptophan supplementation[J].A-mino Acids,2014,46(12):2681-2691.
[49]周次利,陆嫄,吴璐一,等.艾灸生成物干预大鼠尿液代谢组学研究[J].中国组织工程研究,2015,19(15):2387-2393.
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[2]Abu BMH,Sarmidi MR,Cheng KK,et al.Metabolomics-the complementary field in systems biology:a review on obesity and type 2 diabetes[J].Mol Biosyst,2015,11(7):1742-1774.
[3]Fathi F,Ektefa F,Hagh-Azali M,et al.Metabonomics exposes metabolic biomarkers of Crohn's disease by(1)HNMR[J].Gastroenterol Hepatol Bed Bench,2013,6(Suppl 1):S19-22.
[4]邓淑芳,吴巧凤,杨明晓,等.代谢组学技术及其在针灸关键科学问题研究中的应用[J].世界中医药,2015,10(4):472-476.
[5]Bodi V,Marrachelli VG,Husser O,et al.Metabolomics in the diagnosis of acute myocardial ischemia[J].J Cardiovasc Transl Res,2013,6(5):808-815.
[6]Shah NJ,Sureshkumar S,Shewade DG.Metabolomics:A Tool Ahead for Understanding Molecular Mechanisms of Drugs and Diseases[J].Indian J Clin Biochem,2015,30(3):247-54.
[7]Tian Y,Nie X,Xu S,et al.Integrative metabonomics as potential method for diagnosis of thyroid malignancy[J].Sci Rep,2015,5:14869.
[8]Zhang A,Sun H,Qiu S,et al.Metabolomics insights into pathophysiological mechanisms of nephrology[J].Int Urol Nephrol,2014,46(5):1025-1030.
[9]Ma H,Liu X,Wu Y,et al.The Intervention Effects of Acupuncture on Fatigue Induced by Exhaustive Physical Exercises:A Metabolomics Investigation[J].Evid Based Complement Alternat Med,2015,2015:508302.
[10]王金海,杜小正,方晓丽,等.代谢组学技术在针灸研究中的应用探讨[J].甘肃中医学院学报,2012,29(1):18-20.
[11]Zhang Y,Wang N,Zhang M,et al.Metabonomics study on Polygonum multiflorum induced liver toxicity in rats by GC-MS[J].Int JClin Exp Med,2015,8(7):10986-10992.
[12]高骏,刘旭光,颜贤忠,等.代谢组学研究针灸关键问题的初步策略分析与探讨[J].针刺研究,2011,36(4):296-301.
[13]陆嫄,吴璐一,黄任佳,等.基于代谢组学技术研究艾灸治疗溃疡性结肠炎的思考[J].环球中医药,2013,6(6):450-452.
[14]李姝婧.隔药灸脐法与针刺三阴交治疗原发性痛经的代谢组学对比研究[D].济南:山东中医药大学,2013.
[15]杨星月,马玉侠,杜冬青,等.基于代谢组学的隔药灸脐法治疗原发性痛经的机理研究[J].上海针灸杂志,2015,34(8):707-710.
[16]韩媛媛,马玉侠,王晓翠.隔药灸脐法治疗脾虚型肠易激综合征的尿液代谢组学机制[J].广州中医药大学学报,2014,31(4):541-544.
[17]李春静.隔药灸脐法治疗脾虚型肠易激综合征的代谢组学机制研究[D].济南:山东中医药大学,2013.
[18]王晓翠.隔药灸脐法治疗脾虚型肠易激综合征的代谢组学研究[D].济南:山东中医药大学,2012.
[19]张晓宁,马玉侠.隔药灸脐与匹维溴铵对脾虚型肠易激综合征患者尿液代谢组学影响的异同[J].西部中医药,2015,28(7):1-4.
[20]吴巧凤,杨阳,赵纪岚,等.基于~1HNMR代谢组学技术研究针刺经穴与非经穴治疗溃疡性结肠炎的脑代谢物质基础[J].北京中医药大学学报,2014,37(8):572-576+579.
[21]Jiang N,Yan X,Zhou W,et al.NMR-based metabonomic investigations into the metabolic profile of the senescence-accelerated mouse[J].J Proteome Res,2008,7(9):3678-3686.
[22]Yan G,Zhang A,Sun H,et al.Dissection of Biological Property of Chinese Acupuncture Point Zusanli Based on Long-Term Treatment via Modulating Multiple Metabolic Pathways[J].Evid Based Complement Alternat Med,2013,2013:429703.
[23]Gao Z,Liu X,Yu S,et al.Electroacupuncture at Acupoints Reverses Plasma Glutamate,Lipid,and LDL/VLDL in an Acute Migraine Rat Model:A(1)H NMR-Based Metabolomic Study[J].Evid Based Complement Alternat Med,2014,2014:659268.
[24]Angelos PC,Brennan TE,Toriumi DM.Biomechanical properties of superficial musculoaponeurotic system tissue with vs without reinforcement with poly-4-hydroxybutyric acid absorbable mesh[J].JA-MA Facial Plast Surg,2014,16(3):199-205.
[25]Gibson KM,Hoffmann GF,Hodson AK,et al.4-Hydroxybutyric acid and the clinical phenotype of succinic semialdehyde dehydrogenase deficiency,an inborn error of GABA metabolism[J].Neuropediatrics,1998,29(1):14-22.
[26]Boonstra E,de Kleijn R,Colzato LS,et al.Neurotransmitters as food supplements:the effects of GABA on brain and behavior[J].Front Psychol,2015,6:1520.
[27]Pamenter ME,Hogg DW,Ormond J,et al.Endogenous GABA(A)and GABA(B)receptor-mediated electrical suppression is critical to neuronal anoxia tolerance[J].Proc Natl Acad Sci U S A,2011,108(27):11274-11279.
[28]Gilhotra N,Dhingra D.Thymoquinone produced antianxiety-like effects in mice through modulation of GABA and NO levels[J].Pharmacol Rep,2011,63(3):660-669.
[29]Peitzsch M,Prejbisz A,KroiβM,et al.Analysis of plasma 3-methoxytyramine,normetanephrine and metanephrine by ultraperformance liquid chromatography-tandem mass spectrometry:utility for diagnosis of dopamine-producing metastatic phaeochromocytoma[J].Ann Clin Biochem,2013,50(Pt 2):147-155.
[30]Liaudet L,Calderari B,Pacher P.Pathophysiological mechanisms of catecholamine and cocaine-mediated cardiotoxicity[J].Heart Fail Rev,2014,19(6):815-824.
[31]Mullins GR,Wang L,Raje V,et al.Catecholamine-induced lipolysis causes m TOR complex dissociation and inhibits glucose uptake in adipocytes[J].Proc Natl Acad Sci U S A,2014,111(49):17450-17455.
[32]Mizuta K,Zhang Y,Xu D,et al.The dopamine D1 receptor is expressed and facilitates relaxation in airway smooth muscle[J].Respir Res,2013,14:89.
[33]Park JM,Choi SU.Identification of a novel unpaired histidine sensor kinase affecting secondary metabolism and morphological differentiation in Streptomyces acidiscabies ATCC 49003[J].Folia Microbiol(Praha),2015,60(4):279-287.
[34]Fogel WA,Michelsen KA,Granerus G,et al.Neuronal storage of histamine in the brain and tele-methylimidazoleacetic acid excretion in portocaval shunted rats[J].J Neurochem,2002,80(3):375-382.
[35]Zhao C,Du H,Xu L,et al.Metabolomic analysis revealed glycylglycine accumulation in astrocytes after methionine enkephalin administration exhibiting neuron protective effects[J].J Pharm Biomed A-nal,2015,115:48-54.
[36]de Sousa RT,Streck EL,Forlenza OV,et al.Regulation of leukocyte tricarboxylic acid cycle in drug-na?ve Bipolar Disorder[J].Neurosci Lett,2015,605:65-68.
[37]Dong Q,Zhang X,Yu Z,et al.Synthesis reduction of central neurotransmitter 5-hydroxytryptamine by branched chain amino acid and associated antagonists improves postoperative fatigue syndrome[J].Chinese Journal of Gastrointestinal Surgery,2014,17(10):985-989.
[38]Ruan Z,Yang Y,Zhou Y,et al.Metabolomic analysis of amino acid and energy metabolism in rats supplemented with chlorogenic acid[J].Amino Acids,2014,46(9):2219-2229.
[39]Zhang A,Sun H,Wang Z,et al.Metabolomics:towards understanding traditional Chinese medicine[J].Planta Med,2010,76(17):2026-2035.
[40]Liang F,Koya D.Acupuncture:is it effective for treatment of insulin resistance?[J].Diabetes Obes Metab,2010,12(7):555-569.
[41]Wu Q,Zhang Q,Sun B,et al.1H NMR-based metabonomic study on the metabolic changes in the plasma of patients with functional dyspepsia and the effect of acupuncture[J].J Pharm Biomed Anal,2010,51(3):698-704.
[42]Wu QF,Mao S,Cai W,et al.Effects of electroacupuncture of“Weishu”(BL 21)and“Zhongwan”(CV 12)on serum large molecular metabolites in functional dyspepsia rats[J].Acupuncture Research,2010,35(4):287-292.
[43]陈大帅.针刺天枢穴治疗功能性便秘的~1H NMR代谢组学研究[D].成都:成都中医药大学,2014.
[44]Zhang L,Wang Y,Xu Y,et al.Metabonomic analysis reveals efficient ameliorating effects of acupoint stimulations on the menopause-caused alterations in mammalian metabolism[J].Sci Rep,2014,4:3641.
[45]Lee CC,Kasa-Vubu JZ,Supiano MA.Differential effects of raloxifene and estrogen on insulin sensitivity in postmenopausal women[J].JAm Geriatr Soc,2003,51(5):683-688.
[46]Yeh PS,Lin HJ,Chen PS,et al.Effect of statin treatment on threemonth outcomes in patients with stroke-associated infection:a prospective cohort study[J].Eur J Neurol,2012,19(5):689-695.
[47]Munshi SU,Taneja S,Bhavesh NS,et al.Metabonomic analysis of hepatitis E patients shows deregulated metabolic cycles and abnormalities in amino acid metabolism[J].J Viral Hepat,2011,18(10):e591-602.
[48]Ruan Z,Yang Y,Wen Y,et al.Metabolomic analysis of amino acid and fat metabolism in rats with L-tryptophan supplementation[J].A-mino Acids,2014,46(12):2681-2691.
[49]周次利,陆嫄,吴璐一,等.艾灸生成物干预大鼠尿液代谢组学研究[J].中国组织工程研究,2015,19(15):2387-2393.
[50]周思远.针刺胃俞募穴治疗功能性消化不良的~1H NMR代谢组学研究[D].成都:成都中医药大学,2011.
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