石榴籽油在D-半乳糖诱导的衰老小鼠体内的抗氧化作用
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摘要
研究了石榴籽油(PGSO)在D-半乳糖诱导的衰老小鼠体内的抗氧化作用。将60只昆明小鼠随机分为6组:健康组、模型组、维生素E(V_E)阳性对照组以及PGSO低、中、高剂量组,持续给药45 d后,测定各组小鼠的体重,分析PGSO对小鼠肝、肾、脑与血清中抗氧化系统的作用;检测各组小鼠肝、肾中G6PD的活性和NADPH含量。结果表明:与模型组比较,PGSO可拮抗小鼠体重的减轻,降低肝、肾、脑及血清中MDA含量,增加GSH含量,提高T-AOC活性以及抗氧化酶SOD和GSH-Px的活性;且PGSO各剂量组小鼠肝和肾中G6PD的活性均增强,NADPH含量均增加。研究表明PGSO对D-半乳糖诱导的衰老小鼠体内的氧化应激具有明显的拮抗作用。
The antioxidant activity of Punica granatum seed oil(PGSO)on aging model mice induced by D-galactose was studied.Sixty Kunming mice were randomly divided into six groups:healthy group,model group,vitamin E(V_E)positive control group,and low-,medium-,and high-dose PGSO groups.After 45 d treatment,body weights of mice in each group were measured.The effects of PGSO on antioxidant system in liver,kidney,brain and serum of mice were analyzed.And the activity of G6PD and the content of NADPH in liver and kidney of mice were examined.The results showed that PGSO antagonized the decrease of body weight of mice compared with model group.In liver,kidney,brain and serum of mice,PGSO reduced the content of MDA and increased the content of GSH and activity of T-AOC,as well as the activities of antioxidant enzymes SOD and GSH-Px.In addition,in liver and kidney of mice,PGSO elevated the activity of G6PD and the content of NADPH.Taken together,the data indicated that PGSO had the capacity of antagonizing oxidative stress in aging model mice induced by D-galactose.
The antioxidant activity of Punica granatum seed oil(PGSO)on aging model mice induced by D-galactose was studied.Sixty Kunming mice were randomly divided into six groups:healthy group,model group,vitamin E(V_E)positive control group,and low-,medium-,and high-dose PGSO groups.After 45 d treatment,body weights of mice in each group were measured.The effects of PGSO on antioxidant system in liver,kidney,brain and serum of mice were analyzed.And the activity of G6PD and the content of NADPH in liver and kidney of mice were examined.The results showed that PGSO antagonized the decrease of body weight of mice compared with model group.In liver,kidney,brain and serum of mice,PGSO reduced the content of MDA and increased the content of GSH and activity of T-AOC,as well as the activities of antioxidant enzymes SOD and GSH-Px.In addition,in liver and kidney of mice,PGSO elevated the activity of G6PD and the content of NADPH.Taken together,the data indicated that PGSO had the capacity of antagonizing oxidative stress in aging model mice induced by D-galactose.
引文
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[2]余强,聂少平,李文娟,等.灵芝多糖对D-半乳糖致衰老小鼠的作用研究[J].食品科学,2009,30(17):305-307.
[3]LIAO J,WANG K,YAO W,et al.Cloning,expression and antioxidant activity of a thioredoxin peroxidase from Branchiostoma belcheri tsingtaunese[J].Plos One,2017,12(4):1-12.
[4]CHU Y L,YING M L,LIU Z H,et al.Effect of Dendrobium officinale on D-galactose-induced aging mice[J].Chin J Integr Med,2017(1):1-9.
[5]钱宗耀,步岩刚,宋斌.气-质联用测定新疆石榴籽油中脂肪酸含量[J].粮油食品科技,2016,24(1):68-71.
[6]李文敏,敖明章,余龙江,等.石榴籽油的微波提取和体外抗氧化作用研究[J].天然产物研究与开发,2006,18(3):378-380.
[7]王毓宁,何静,李鹏霞,等.石榴籽油自由基清除能力及对H2O2诱导PC12细胞损伤的保护作用[J].中国食品学报,2016,16(5):32-36.
[8]马齐,秦涛,王丽娥,等.石榴籽油的提取及成分分析[J].粮油食品科技,2008,16(1):28-30.
[9]邓红,范雪层,田子卿,等.文冠果种仁冷榨油体内抗氧化功能研究[J].中国油脂,2010,35(12):38-40.
[10]田华,向红莉,付杰,等.DHA藻油调和油对SD大鼠心肌组织的影响[J].中国油脂,2016,41(8):54-57.
[11]胡滨,陈一资,苏赵.西瓜籽油辅助降血脂功能研究[J].中国油脂,2017,42(2):56-62.
[12]苗利利,吴浩浩,仇农学,等.石榴籽油的体内抗氧化性评价[J].中国油脂,2010,35(1):37-40.
[13]王美菊,陶明煊,牛文颖,等.石榴叶多酚对急性酒精性肝损伤小鼠肾脏、心脏及免疫器官的抗氧化作用[J].食品科学,2016,37(1):208-212.
[14]KUMER D,RIZVI S I.Black tea supplementation augments redox balance in rats:relevance to aging[J].Arch Physiol Biochem,2017,23:1-7.
[15]刘贵珊,杨博,张泽生,等.白藜芦醇对D-半乳糖致衰老小鼠学习记忆能力和脑组织抗氧化能力的影响[J].食品科学,2014,35(5):204-207.
[16]吴莹,周庆,李杏,等.蓝莓花青素与白藜芦醇复配对老龄小鼠抗衰老作用的评价[J].中国医院药学杂志,2016,36(24):2178-2183.
[17]贾秀月,高艳华,赵晓莲,等.自由基与抗衰老的研究概况[J].黑龙江医药科学,2007,30(2):75-76.
[18]李书丹,宋娜,李晓东,等.红树莓原液对D-半乳糖模型小鼠抗衰老作用[J].长春中医药大学学报,2016,32(5):902-904.
[19]甄天元,赵梦醒,雷敏.鱿鱼墨黑色素对亚急性衰老模型小鼠抗氧化功能的影响[J].中国食品学报,2012,12(5):61-65.
[20]ZHANG W K,TAO S S,LI T T,et al.Nutmeg oil alleviates chronic inflammatory pain through inhibition of COX-2 expression and substance P release in vivo[J].Food Nutr Res,2016,60:1-5.
[21]QIN Y Y,LI M,FENG X,et al.Combined NADPH and the NOX inhibitor apocynin provides greater anti-inflammatory and neuroprotective effects in a mouse model of stroke[J].Free Radical Bio Med,2017,104:333-345.
[22]NAGASAKIAKI H,NAKASHIMA A,KANEKO S,et al.Aripiprazole increases NADPH level in PC12 cells:the role of NADPH oxidase[J].J Neural Transm,2014,121(1):91-103.