砖红壤微生物总DNA的提取方法比较

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英文篇名：Comparison of Extraction Methods for Total DNA of Microbes from Latosols 作者：邓大豪 ; 邓涛 ; 周游 ; 汪军 ; 杨腊英 ; 黄俊生 英文作者：DENG Dahao;DENG tao;ZHOU you;WANG jun;YANG Laying;HUANG Junsheng;Institute of Environment and Plant Protection,CATAS;Hainan University; 关键词：土壤微生物 ; DNA提取 ; 16S ; rDNA ; ITS ; rDNA 英文关键词：soil microbe;;DNA extraction;;16S rDNA;;ITS rDNA 中文刊名：RDNK 英文刊名：Chinese Journal of Tropical Agriculture 机构：中国热带农业科学院环境与植物保护研究所;海南大学; 出版日期：2019-01-25 出版单位：热带农业科学 年：2019 期：v.39;No.257 基金：国家重点研发计划项目(No.2017YFD020060206);; 海南自然科学基金创新研究团队项目(No.2017CXTD016);; 中国热带农业科学院基本科研业务费专项资金(No.1630042018013);中国热带农业科学院环境与植物保护研究所自主选题项目(No.hzsjy2017004);; 广西创新驱动发展专项资金项目(No.桂科AA18118011-2) 语种：中文; 页：RDNK201901010 页数：5 CN：01 ISSN：46-1038/S 分类号：50-54

摘要

针对中国南方砖红土壤的特点,通过比较和优化土壤微生物总DNA的提取方法,获得较高质量的DNA,从而进行土壤细菌16S r DNA基因扩增和真菌I TS r DNA基因扩增。结果表明:方法一提取的土壤微生物DNA受到腐殖质等影响无法进行基因扩增;方法二提取的DNA可以进行土壤微生物基因组16S r DNA基因扩增,提取时间大大减少,但无法进行I TS r DNA基因扩增;方法三提取的DNA质量最好,可以进行细菌16S r DNA和真菌I TS r DNA基因组扩增。
        According to the c haracteristics of latosols in southern China, extraction methods for soil microbial total DNA were compared and optimized to produce high quality total DNA s from the soil microbes in latosols, and the soil bacteria were identified by using the 16 S r DNA amplification and the soil fungi by using ITS r DNA. The results showed that the soil microbial DNA extracted by the first method could not be amplified by the ITS and 16 s primers because of the existence of humus and others.The DNA extracted by the second method could be used to amplify the 16 S r DNA gene of soil microbial genome with shorter extraction time, but the ITS r DNA gene amplification could not be performed. The extracted DNA by the third method was best in quality and could be used for bacterial and fungal genome amplification.

引文

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