红树林植物阔苞菊内生真菌硒富集工艺优化
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摘要
为了获得有机硒的来源新途径,以菌丝体的富硒总量为指标,采用单因素、正交实验法研究了一株阔苞菊内生真菌的硒富集能力,对培养基中硒溶液添加量、盐浓度、碳源浓度、培养时间、碳源种类等影响因素进行了优化。结果表明,适合的培养条件为硒溶液添加量为30 m L、盐浓度5 g/L、碳源(葡萄糖)浓度15 g/L、培养时间24 h;代谢产物中硒含量为181.6μg/g,菌丝体富硒总量为1941.7μg/L,其有机硒转化率偏低,仅为16.2%。为进一步筛选开发滨海红树林中内生富硒微生物资源提供理论依据。
Study of the selenium enrichment ability for an mangrove plant Pluchea indica endophytic fungus by means of single factor and orthogonal experiments,in order to obtain organic selenium.The influence factors such as selenium solution added amount,salt concentration,carbon source concentration,culture time and carbon source were optimized with the total selenium accumulation as the index in the experiment. The results showed that when the enriched culture medium was contained salt concentration 5 g/L,selenium solution added volume 30 m L,carbon source( glucose) concentration 15 g/L,culture time 24 h,the total selenium accumulation was 1941.7 g/L,the mycelium Se content of up to 181.6 μg/g and the lower organic selenium transformation rate was 16.2%. It is expected to provide theoretical basis for further researching selenium rich microbial resources in coastal mangrove plants.
Study of the selenium enrichment ability for an mangrove plant Pluchea indica endophytic fungus by means of single factor and orthogonal experiments,in order to obtain organic selenium.The influence factors such as selenium solution added amount,salt concentration,carbon source concentration,culture time and carbon source were optimized with the total selenium accumulation as the index in the experiment. The results showed that when the enriched culture medium was contained salt concentration 5 g/L,selenium solution added volume 30 m L,carbon source( glucose) concentration 15 g/L,culture time 24 h,the total selenium accumulation was 1941.7 g/L,the mycelium Se content of up to 181.6 μg/g and the lower organic selenium transformation rate was 16.2%. It is expected to provide theoretical basis for further researching selenium rich microbial resources in coastal mangrove plants.
引文
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[2]秦俊法.中国硒研究历史回顾(上)[J].广东微量元素科学,2014,21(11):44-57.
[3]秦俊法.中国硒研究历史回顾(下)[J].广东微量元素科学,2014,21(12):36-51.
[4]Tan J A,Zhu W Y,Wang W Y,et al.Selenium in soil and endemic diseases in China[J].The Science of the Total Environment,2002,284(1):227-235.
[5]Abdulah R,Miyazaki K,Nakazawa M,et al.Chemical forms of selenium for cancer prevention[J].Journal of Trace Elements in Medicine and Biology,2005,19(2):141-150.
[6]姚昭,李红艳,张云龙,等.有机硒、无机硒、VE单独使用及有机硒与VE联用对大鼠体内抗氧化能力的影响[J].食品科学,2013,34(15):272-276.
[7]高慧娟,杨霞霞,杜学基,等.荷叶离褶伞菌丝体富硒条件的研究[J].食品工业科技,2013,34(11):146-149.
[8]Marek K,Stanislaw B,Iwona G..Acucmulation and metabolism of selenium by yeast cells[J].Applied Microbiology and Biotechnology,2015,99(13):5373-5382.
[9]梁金钟,王翼雪,梅剑秋.植物乳杆菌富硒培养条件的优化[J].食品工业科技,2017,38(3):137-142.
[10]李祎,郑伟,郑天凌.海洋微生物多样性及其分子生态学研究进展[J].微生物学通报,2013,40(4):655-668.
[11]张偲,张长生,田新朋,等.中国海洋微生物多样性研究[J].中国科学院院刊,2010,25(6):651-658.
[12]杨志强,李杰,郑国东,等.广西北部湾沿海经济区富硒土壤地球化学特征[J].物探与化探,2014,38(6):1260-1264.
[13]徐树兰,程中山,陈其津,等.不同培养条件下红树林真菌1893菌株代谢产物的杀虫活性[J].环境昆虫学报,2009,31(1):58-62.
[14]杨革.灵芝菌丝体深层培养及多糖提取工艺研究[J].生物学杂志,1997,14(2):16-17.
[15]蒋步云,柴振林,吕爱华,等.杨梅中硒含量测定的消化方法[J].浙江农业科学,2012,8(14):1175-1176.
[16]郝素娥,滕冰.硒酵母中硒含量测定方法的研究[J].理化检验,2012,4(8):151-153.
[17]刘娟,焦华.分光光度法测定大米中硒含量[J].光谱实验室,2012,4(4):2376-2379.
[18]杨永锋,姚耀柱,梁嘉梨,等.两种干海产品中硒含量的紫外-可见分光光度间接测定[J].化学研究与应用,2016,28(6):837-841.
[19]Michael O G,Robert C.E.Fungal Nutrition and Physiology[M].USA:A Wiley-Interscience Publication,1984:71-96.
[20]李艳宾,张琴.产黄酮甘草内生真菌YF-A的发酵工艺优化[J].食品科技,2016,41(11):25-30.
[21]Schubert A,Allara P,Morte A.Cleavage of sucrose in roots of soybean(Glycine max)colonized by an arbuscular mycorrhizal fungus[J].New Phytologist,2004,161:495-501.
[22]邹英宁,吴强盛,李艳,等.丛枝菌根真菌对枳根系形态和蔗糖、葡萄糖含量的影响[J].应用生态学报,2014,25(4):1125-1129.
[23]陈志杰,袁书林.食(药)用真菌富硒及产品开发研究进展[J].中国食用菌,2009,28(6):9-11.