正常脑组织和脑卒中组织的电阻抗频谱特性研究
|
摘要
目的:测量和分析正常脑组织(白质和灰质)和脑卒中病变组织(出血组织和缺血组织)的阻抗频谱特性,为开展多频电阻抗成像(electrical impedance tomography,EIT)检测脑卒中研究提供可靠的数据支持。方法:首先,采用自体血注入法和光化学诱导法分别建立家兔脑出血模型和脑缺血模型;其次,分别测量离体15 min内的脑白质、灰质、脑出血组织和脑缺血组织在10 Hz～1 MHz内的阻抗频谱;最后,对比分析正常脑组织和脑卒中组织在10 Hz～1 MHz内的差异。结果:在整个频段内,正常脑组织和脑卒中组织的阻抗频谱存在明显的差异(在10 Hz～1 MHz内,电导率谱差异大于10%),2种卒中组织的阻抗频差变化也明显不同(在100 k Hz内,出血组织的电导率几乎保持不变,而缺血组织呈线性增加)。结论:从组织阻抗频谱特性的角度进一步证实了多频EIT检测脑卒中的可行性,同时为未来开展多频EIT检测脑卒中研究奠定了良好的数据基础。
Objective To measure the electrical impedance spectrum properties of normal brain tissue(gray and white matters) and stroke tissue(hemorrhagic and ischemia tissue) in order to provide reliable data support for studying stroke detection using multi-frequency electrical impedance tomography(EIT). Methods First, the rabbit cerebral hemorrhage model and ischemia model were established using autologous blood injection and photochemical induction, respectively;second, the impedance spectra of white matter, gray matter, hemorrhagic tissue and ischemic tissue within 15 min after animal death at frequencies from 10 Hz to 1 MHz were measured; third, the difference in impedance spectra between normal brain tissue and stroke tissue was compared. Results There was a significant difference in the impedance spectra between normal brain tissue and stroke tissue throughout the frequency band(within 10 Hz-1 MHz, the conductivity difference was greater than 10%); the impedance change with frequency between the two kinds of stroke tissues were also significantly different(below 100 k Hz, the conductivity of the hemorrhagic tissue remained almost unchanged while the conductivity of the ischemic tissue increased linearly). Conclusion This study further confirms the feasibility of using multi-frequency EIT to detect stroke from the perspective of tissue impedance spectrum property and lays a solid data foundation for future study of stroke detection using multi-frequency EIT.
Objective To measure the electrical impedance spectrum properties of normal brain tissue(gray and white matters) and stroke tissue(hemorrhagic and ischemia tissue) in order to provide reliable data support for studying stroke detection using multi-frequency electrical impedance tomography(EIT). Methods First, the rabbit cerebral hemorrhage model and ischemia model were established using autologous blood injection and photochemical induction, respectively;second, the impedance spectra of white matter, gray matter, hemorrhagic tissue and ischemic tissue within 15 min after animal death at frequencies from 10 Hz to 1 MHz were measured; third, the difference in impedance spectra between normal brain tissue and stroke tissue was compared. Results There was a significant difference in the impedance spectra between normal brain tissue and stroke tissue throughout the frequency band(within 10 Hz-1 MHz, the conductivity difference was greater than 10%); the impedance change with frequency between the two kinds of stroke tissues were also significantly different(below 100 k Hz, the conductivity of the hemorrhagic tissue remained almost unchanged while the conductivity of the ischemic tissue increased linearly). Conclusion This study further confirms the feasibility of using multi-frequency EIT to detect stroke from the perspective of tissue impedance spectrum property and lays a solid data foundation for future study of stroke detection using multi-frequency EIT.
引文
[1] GO A S,DARIUSH M,ROGER V L,et al. Executive summary:heart disease and stroke statistics—2013 update:a report from the American Heart Association[J]. Circulation,2015,127(1):143-152.
[2] DEL ZOPPO G J,SAVER J L,JAUCH E C,et al. Expansion of the time window for treatment of acute ischemic stroke with intravenous tissue plasminogen activator:a science advisory from the American Heart Association/American Stroke Association[J]. Stroke,2009,40(8):2 945-2 948.
[3] SUROWIEC A,STUCHLY S S,SWARUPA. Postmortem changes of the dielectric properties of bovine brain tissues at low radiofrequencies[J]. Bioelectromagnetics,1986,7(1):31-43.
[4] GABRIEL S,LAU R W,GABRIEL C. The dielectric properties of biological tissues:Ⅲ. Parametric models for the dielectric spectrum of tissues[J]. Phys Med Biol,1996,41(11):2 271-2 293.
[5]吴小明,董秀珍,秦明新,等.家兔脑组织复电阻抗频率特性及其等效电路模型[J].中国生物医学工程学报,2003,22(3):228-234.
[6] DOWRICK T,BLOCHET C,HOLDER D. In vivo bioimpedance measurement of healthy and ischaemic rat brain:implications for stroke imaging using electrical impedance tomography[J]. Physiol Meas,2015,36(6):1 273.
[7]杨亚萍,刘晓鹏,台立稳.自体血注入法大鼠脑出血模型建立的制作技巧[J].神经药理学报,2012,2(2):29-31.
[8]张杰文,索爱琴,张茂悦,等.光化学诱导大鼠局灶脑缺血动物模型的建立及磁共振T2WI检测[J].骨科,2000,24(4):179-180.
[9]李衡,董秀珍,祁琦,等.脑部电阻抗测量中电极与导电膏性能对比研究[J].医疗卫生装备,2017,38(1):1-5.
[10]蔡占秀,邱召运,连波,等.甲状腺正常组织介电特性的研究[J].医疗卫生装备,2016,37(12):13-16.
[11]王航,史学涛,董秀珍.基于comsol有限元分析的离体组织阻抗测量盒的仿真研究[J].医疗卫生装备,2010,31(11):178-181.
[12]朱建波,史学涛,尤富生,等.生物组织活性与介电特性关系的探索研究[J].医疗卫生装备,2013,34(1):1-3.
[2] DEL ZOPPO G J,SAVER J L,JAUCH E C,et al. Expansion of the time window for treatment of acute ischemic stroke with intravenous tissue plasminogen activator:a science advisory from the American Heart Association/American Stroke Association[J]. Stroke,2009,40(8):2 945-2 948.
[3] SUROWIEC A,STUCHLY S S,SWARUPA. Postmortem changes of the dielectric properties of bovine brain tissues at low radiofrequencies[J]. Bioelectromagnetics,1986,7(1):31-43.
[4] GABRIEL S,LAU R W,GABRIEL C. The dielectric properties of biological tissues:Ⅲ. Parametric models for the dielectric spectrum of tissues[J]. Phys Med Biol,1996,41(11):2 271-2 293.
[5]吴小明,董秀珍,秦明新,等.家兔脑组织复电阻抗频率特性及其等效电路模型[J].中国生物医学工程学报,2003,22(3):228-234.
[6] DOWRICK T,BLOCHET C,HOLDER D. In vivo bioimpedance measurement of healthy and ischaemic rat brain:implications for stroke imaging using electrical impedance tomography[J]. Physiol Meas,2015,36(6):1 273.
[7]杨亚萍,刘晓鹏,台立稳.自体血注入法大鼠脑出血模型建立的制作技巧[J].神经药理学报,2012,2(2):29-31.
[8]张杰文,索爱琴,张茂悦,等.光化学诱导大鼠局灶脑缺血动物模型的建立及磁共振T2WI检测[J].骨科,2000,24(4):179-180.
[9]李衡,董秀珍,祁琦,等.脑部电阻抗测量中电极与导电膏性能对比研究[J].医疗卫生装备,2017,38(1):1-5.
[10]蔡占秀,邱召运,连波,等.甲状腺正常组织介电特性的研究[J].医疗卫生装备,2016,37(12):13-16.
[11]王航,史学涛,董秀珍.基于comsol有限元分析的离体组织阻抗测量盒的仿真研究[J].医疗卫生装备,2010,31(11):178-181.
[12]朱建波,史学涛,尤富生,等.生物组织活性与介电特性关系的探索研究[J].医疗卫生装备,2013,34(1):1-3.