秦巴山地玉米种质资源的SSR标记遗传多样性分析
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摘要
为了明确秦巴山地玉米自交系的亲缘关系,采用SSR分子标记方法对26份玉米自交系进行了遗传多样性分析,以遗传相似系数0.624 5为标准,将自交系分为5个类群,初步确定其遗传关系。结果表明:在供试材料中共检测到212个等位基因变异,平均每对引物检测到的等位基因变异为8.83个,变异为4～11个,平均多态性信息量PIC值为0.75,变化范围为0.31～0.93;自交系HSZ-3R被划分到第Ⅲ类群里,与其他25份材料的遗传相似系数范围在0.407 9～0.671 1之间,HSZ-3R与其他4个类群的玉米自交系配置组合具有一定的杂种优势;自交系HB41065被划分到第Ⅴ类群,与其余25份参试自交系材料的遗传相似系数为0.402 5～0.600 0,遗传距离较远,杂种优势较强,在秦巴玉米组合选配中可作为重点材料加以利用。
In order to clarify the genetic relationship of maize inbred lines in the Qinba mountain, the genetic diversity of 26 inbred lines was analyzed through the SSR molecular marker method. Those inbred lines were divided into 5 groups by the genetic similarity coefficient0.624 5 as the criterion. The genetic relationship and genetic distance of these self-crossing materials in different groups were identified to provide an reference for the rational use in breeding of strong advantage combinations. The results showed that a total of 212 alleles were detected in the test materials. The average number of allele detected by per pair of primers was 8.83, the variation was 4 to 11, and the average polymorphic information content PIC value was 0.75. The range of changes was 0.31 to 0.93. The self-cross line HSZ-3 R was divided into Class III group, and the genetic similarity coefficients between HSZ-3 R and other 25 materials ranged from 0.407 9 to 0.671 1;its hybrid combinations with the inbred lines from other 4 groups had certain heterosis advantages. The inbred line HB41065 was divided into Group V, and the genetic similarity coefficients between HB41065 and 25 other inbred lines were 0.402 5～0.600 0, which indicated that the genetic distance was longer and the heterosis was stronger. HB41065 can be used as a key material in maize combination selection in Qinba mountain.
In order to clarify the genetic relationship of maize inbred lines in the Qinba mountain, the genetic diversity of 26 inbred lines was analyzed through the SSR molecular marker method. Those inbred lines were divided into 5 groups by the genetic similarity coefficient0.624 5 as the criterion. The genetic relationship and genetic distance of these self-crossing materials in different groups were identified to provide an reference for the rational use in breeding of strong advantage combinations. The results showed that a total of 212 alleles were detected in the test materials. The average number of allele detected by per pair of primers was 8.83, the variation was 4 to 11, and the average polymorphic information content PIC value was 0.75. The range of changes was 0.31 to 0.93. The self-cross line HSZ-3 R was divided into Class III group, and the genetic similarity coefficients between HSZ-3 R and other 25 materials ranged from 0.407 9 to 0.671 1;its hybrid combinations with the inbred lines from other 4 groups had certain heterosis advantages. The inbred line HB41065 was divided into Group V, and the genetic similarity coefficients between HB41065 and 25 other inbred lines were 0.402 5～0.600 0, which indicated that the genetic distance was longer and the heterosis was stronger. HB41065 can be used as a key material in maize combination selection in Qinba mountain.
引文
[1]刘春晓,李会海,马兰,等.利用SSR标记划分144份玉米自交系的杂种优势群[J].山东农业科学,2018,50(9):1-6.
[2]陈华.利用SSR标记划分我国北方主要玉米自交系杂种优势群[D].新疆:新疆农业大学,2004.
[3]袁力行,傅骏骅,Warburton M.等.利用RFLP、SSR、AFLP和RAPD标记分析玉米自交系遗传多样性的比较研究[J].遗传学报,2000,27:725-733.
[4]赵兴华,王长彪,董艳辉,等. SSR分子标记在玉米育种中的研究进展[J].现代农业科技,2016,7:15-16.
[5]Senior M L,Murphy J P,Goodman M M,et al. Utility of SS R s for determining genetic similarities and relationships in maize using an agarose gel system[J]. Crop Science,1998,38:1088-1098.
[6]吉琼,张新玲,童玲,等.利用SSR标记研究96个玉米自交系的遗传多样性及其群体遗传结构[J].新疆农业大学学报,2012,35(2):99-106.
[7]王慧,卢有林,孙大鹏,等.糯玉米种质品质性状鉴定和SSR标记遗传多样性分析[J].植物遗传资源学报,2013,14(5):800-808.
[8]程宇坤,白建荣,张效梅,等.山西省糯玉米自交系的遗传多样性分析及类群划分[J].山西农业科学,2012,40(5):433-438.
[9]王凤格,田红丽,赵久然,等.中国328个玉米品种(组合)SSR标记遗传多样性分析.中国农业科学,2014,47(5):856-864.
[10]赵久然,王凤格,郭景伦,等.中国玉米新品种DNA指纹库建立系列研究:Ⅱ适于玉米自交系和杂交种指纹图谱绘制的SSR核心引物的确定.玉米科学,2003,11(2):3-5,8.
[11]ZhaoJR,WangFG,GuoJL,etal.Seriesofresearchon establishing DNA fingerprinting pool of Chinese new maize cultivars:Ⅱ. confirmation of a set of SSR core primer pairs[J]. Journal of Maize Sciences,2003,11(2):3-5,8.(in Chinese).
[12]Wang F G,Zhao J R,Dai J R,et al. Selection and development of representative simple sequence repeat primers and multiplex SSR sets for high throughput automated genotyping in maize[J]. Chinese Science Bulletin,2007,52(2):215-223.
[13]Smith J S C,Chin E C L,Shu H,et al. An evaluation of the utility SSRloci as molecular marker in maize:comparisons with date from RFLPs and pedigree[J]. Theor Appl Genet,2009,95:163-173.
[14]Botstein D,Wite R L,Skolnick M, et al. Construction of a genetic linkage map in man using resrriction fragment length polymorphisms[J].Am J Hum Genet,2011,32:314-331.
[2]陈华.利用SSR标记划分我国北方主要玉米自交系杂种优势群[D].新疆:新疆农业大学,2004.
[3]袁力行,傅骏骅,Warburton M.等.利用RFLP、SSR、AFLP和RAPD标记分析玉米自交系遗传多样性的比较研究[J].遗传学报,2000,27:725-733.
[4]赵兴华,王长彪,董艳辉,等. SSR分子标记在玉米育种中的研究进展[J].现代农业科技,2016,7:15-16.
[5]Senior M L,Murphy J P,Goodman M M,et al. Utility of SS R s for determining genetic similarities and relationships in maize using an agarose gel system[J]. Crop Science,1998,38:1088-1098.
[6]吉琼,张新玲,童玲,等.利用SSR标记研究96个玉米自交系的遗传多样性及其群体遗传结构[J].新疆农业大学学报,2012,35(2):99-106.
[7]王慧,卢有林,孙大鹏,等.糯玉米种质品质性状鉴定和SSR标记遗传多样性分析[J].植物遗传资源学报,2013,14(5):800-808.
[8]程宇坤,白建荣,张效梅,等.山西省糯玉米自交系的遗传多样性分析及类群划分[J].山西农业科学,2012,40(5):433-438.
[9]王凤格,田红丽,赵久然,等.中国328个玉米品种(组合)SSR标记遗传多样性分析.中国农业科学,2014,47(5):856-864.
[10]赵久然,王凤格,郭景伦,等.中国玉米新品种DNA指纹库建立系列研究:Ⅱ适于玉米自交系和杂交种指纹图谱绘制的SSR核心引物的确定.玉米科学,2003,11(2):3-5,8.
[11]ZhaoJR,WangFG,GuoJL,etal.Seriesofresearchon establishing DNA fingerprinting pool of Chinese new maize cultivars:Ⅱ. confirmation of a set of SSR core primer pairs[J]. Journal of Maize Sciences,2003,11(2):3-5,8.(in Chinese).
[12]Wang F G,Zhao J R,Dai J R,et al. Selection and development of representative simple sequence repeat primers and multiplex SSR sets for high throughput automated genotyping in maize[J]. Chinese Science Bulletin,2007,52(2):215-223.
[13]Smith J S C,Chin E C L,Shu H,et al. An evaluation of the utility SSRloci as molecular marker in maize:comparisons with date from RFLPs and pedigree[J]. Theor Appl Genet,2009,95:163-173.
[14]Botstein D,Wite R L,Skolnick M, et al. Construction of a genetic linkage map in man using resrriction fragment length polymorphisms[J].Am J Hum Genet,2011,32:314-331.
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