复合水凝胶支架在骨组织工程中的应用
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摘要
大面积的颌骨缺损是口腔医学中的常见临床问题,目前广泛运用骨组织移植、引导骨组织再生技术、牵张成骨的方法修复骨缺损,恢复组织器官的功能与美观,但这些方法依然存在局限性。骨组织工程的出现为骨缺损患者的治疗提供了新选择,其重点是制备支架材料替代移植物,植入缺损区,实现骨愈合。其中,支架材料的选择是组织工程骨再生的核心问题。相较于传统支架材料,水凝胶的机械学性能、生物相容性、生物可降解性具有独特的优势,是理想的骨修复支架材料,在组织工程的应用中,已获得初步进展。近期研究发现,复合水凝胶、环境敏感型水凝胶支架能提高材料表面的细胞粘附并诱导干细胞向成骨方向分化,为指导骨修复提供了全新的策略。
One of the most common clinical problems in Stomatology is the defect of jaw. Currently,methods such as bone tissue transplantation,guided bone tissue regeneration and distraction osteogenesis have been widely used to repair bone defects in order to restore the appearance and functions of the tissues. But they still have their own limitations. Bone tissue engineering provides a new option for the treatment of patients with bone defects. It focuses on how to prepare scaffolds instead of implants,and then transplant the scaffold into the area with bone defect to achieve the purpose of bone regeneration. Among them,the key factor in the success of bone tissue engineering is the choice of scaffold materials. Compared with conventional scaffold materials,hydrogels have unique advantages in terms of mechanical properties,biocompatibility and biodegradability. They are ideal materials that can be used to repair bone defects,and have made some progress in the application of regenerative medicine. Recently,researchers have found that composite hydrogel scaffolds and environment-sensitive hydrogel scaffolds can improve the attachment ability of cells and can make mesenchymal stem cells differentiate into osteoblasts,which provides a new strategy for bone repair.
One of the most common clinical problems in Stomatology is the defect of jaw. Currently,methods such as bone tissue transplantation,guided bone tissue regeneration and distraction osteogenesis have been widely used to repair bone defects in order to restore the appearance and functions of the tissues. But they still have their own limitations. Bone tissue engineering provides a new option for the treatment of patients with bone defects. It focuses on how to prepare scaffolds instead of implants,and then transplant the scaffold into the area with bone defect to achieve the purpose of bone regeneration. Among them,the key factor in the success of bone tissue engineering is the choice of scaffold materials. Compared with conventional scaffold materials,hydrogels have unique advantages in terms of mechanical properties,biocompatibility and biodegradability. They are ideal materials that can be used to repair bone defects,and have made some progress in the application of regenerative medicine. Recently,researchers have found that composite hydrogel scaffolds and environment-sensitive hydrogel scaffolds can improve the attachment ability of cells and can make mesenchymal stem cells differentiate into osteoblasts,which provides a new strategy for bone repair.
引文
[1]Mehrali M,Thakur A,Pennisi CP,et al.Nanoreinforced hydrogels for tissue engineering:biomaterials that are comlatible with load-bearing and electroactive tissue[J].Adv Mater,2017,29(8):612-638.
[2]Rufai0hah AJ,Seliktar D.Hydrogels for therapeutic cardiovascular angiogenesis[J].Adv Drug Deliv Rev,2016,15(96):31-39.
[3]Narayanan G,Vernekar VN,Kuyine EL,et al.Poly(lactic acid)-based biomaterials for orthopaedic regenerative engineering[J].Adv Drug Deliv Rev,2016,15(107):247-276.
[4]Kaqami H,Aqata H,Inoue M,et al.The use of bone marrow stromal cells(bone marrow-derived multipotent mesenchymal stromal cells)for alveolar bone tissue engineering:basic science to clinical translation[J].Tissue Enq Part B Rev,2014,20(3):229-232.
[5]韩浩,李利伟,秦文贞,等.ACF/PLGA骨组织工程复合支架的制备及其性能[J].材料科学与工程学报,2012,30(5):719
[6]杨维东,史培良,杨书勇,等.改善珊瑚表面成骨细胞黏附和增殖的研究[J].解放军医学杂志,2001,26(4):248-250.
[7]Zhang Y,Fu C,Li Y,et al.Synthesis of an injectable,self-healable and dual responsive hydrogel for drug delivery and 3D cell cultivation[J].Polym Chem,2017,8(3):537-544.
[8]Iwata T,Yamato M,Ishikawa I,et al.Tissue engineering in periodontal tissue[J].Anat Rec,2014,297(1):16-25.
[9]Chia HN,Wu BM.Recent advances in 3D printing of biomaterials[J].J Biol Eng,2015,9(1):4-18.
[10]Bose S,Roy M,Bandyopadhyay A.Recent advanced in bone tissue engineering scaffolds[J].Trends Biotechnol,2012,30(10):546-554.
[11]Velasco MA,Narvaez-Tovar CA,Grazon-Alvarado DA.Design,materials,and mechanobiology of biodegradable scaffolds for bone tissue engineering[J].Bio Med Res Inter,2015,2015(1-6):729076.
[12]Murphy CM,O Brien FJ,Little DG,et al.Cell-scaffold interactions in the bone tissue engineering triad[J].Eue Cells Mater,2013,26:120-132.
[13]Lou T,Wang X,Song G,et al.Structure and properties of PLLA/β-TCP nanocomposite scaffolds for bone tissue engineering[J].J Mater Sci Mater Med,2013,26(4):120-132.
[14]Liu Y,Lim J,Teoh SH.Review:Development of clinically relevant scaffolds for vascularised bone tissue engineering[J].Biotechnol Adv,2013,31(5):688-705.
[15]Pakulska MM,Vulic K,Tam RY,et al.Hybrid crosslinked methylcellulose hydrogel:A predictable and tunable platform for local drug delivery[J].Adv Mater,2015,27(34):5002-5008.
[16]Ni M,Zhang N,Xia W,et al.Dramatically promoted swelling of a hydrogel by pillar[6]arene-ferrocene complexation with multistimuli responsiveness[J].J Am Chem Soc,2016,138(20):6643-6649.
[17]Wenz A,Borchers K,Tovar GEM,et al.Bone matrix production in hydroxyapatite-modified hydrogels suitable for bone bioprinting[J].Biofabrication,2017,9(4):103-117.
[18]Tijore A,Irvine SA,Sariq U,et al.Contact guidence for cardiac tissue engineering using 3D bioprinted gelatin patterned hydrogel[J].Biofabrication,2017,12(13):228-267.
[19]Huang J,Jiang X.Injectable and degradable p H-responsive hydrogelvia spontaneous amino-yne click reaction[J].ACS Appl Mater Interfaces,2017,12(13):2-35.
[20]Chen W,Hao D,Hao W,et al.Hydrogel with ultra-fast selfhealing property both in air and underwater[J].ACS Appl Mater Interfaces,2017,11(13):18-45.
[21]Khorshidi S,Karkhaneh A.Hydrogel/fiber conductive scaffold for bone tissue engineering[J].J Biomed Mater Res A,2017,9(1):82-89.
[22]Gsib O,Duval JL,Goczkowski M,et al.Evaluation of fibrinbased interpenetrating polymer networks as potential biomaterials for tissue engineering[J].Nanomaterials,2017,7(12):436-457.
[23]Garza EB,Gomez S,Chayet A,et al.One-year safety and efficacy resultss of a hydrogel inlay to improve near vision in patients with emmetropic presbyopia[J].J Refract Surg,2013,29(3):166
[24]张磊,丁琪,夏扬,等.掺杂Fe3O4磁性纳米粒子壳聚糖凝胶的制备及其性质研究[J].东南大学学报,2012,31(5):567-571.
[25]Salick DA,Pochan DJ,Schneider JP.Design of an injectableβ-hairpin peptide hydrogel that kills methicillin-resistant staphylococcus aureus[J].Adv Mater,2010,21,21(41):4120-4123.
[26]Hoemann CD,Sun J,Legare A,et al.Tissue engineering of cartilage using an injectable and adhesive chitosan-based cell-delivery vehicle[J].Osteoarthritis Cartlage,2005,13(4):318-329.
[27]Hoemann CD,Chenite A,Sun J,et al.Cytocompatible gel formation of chitosan-glycerol phosphate solutions supplemented with hydroxyl ethyl cellulose is due to the presence of glyoxal[J].J Biomed Mater Res,2007,83(2):521-529.
[28]Chao PH,Yodmuang S,Wang X,et al.Silk hydrogel for cartliage tissue enginee-ring[J].J Biomed Mater Res,2010,95b(1):84-90.
[29]Yue K,Santiago GT,Alvarez MM,et al.Synthesis,properties,and biomedical applications of gelatin methacryloyl(Gel MA)hydrogels[J].Biomaterials,2015,73(12):8701-8707.
[30]Frasca S,Norol F,Visage CL,et al.Calcium-phosphate ceramics and polysaccharide-based hydrogel scaffolds combined with mesenchymal stem cell with mesenchymal stem cell differently support bone repair in rats[J].J Mate Sci Mater Med,2017,28(2):35-48.
[31]Zhao L,Wang LM,Detamore MS.Human umbilical cord mesenchymal stromal cell-based approach for osteochondral tissue engineering[J].J Tissue Eng Regen M,2011,5(9):712-721.
[32]何大为,金岩,骆凯,等.重组人骨形成蛋白-2/转化生长因子-β作用下陶瓷化骨粉/水凝胶与骨髓基质细胞修复自体颅骨缺损[J].中国临床康复,2005,9(46):339-341.
[33]Pappas NA,Hilt JZ,Khademhosseini A,et al.Hydrogels in biology and medicine:from molecular principles to bionanotechnology[J].Adv Mater,2006,18(11):1345-1366.
[34]Rowley JA,Madlambayan G,Mooney DJ.Alginate hydrogels as cynthetic extracellular matrix materials[J].Biomaterials,1999,20(1):45-53.
[35]Karp JM,Sarraf F,Shoichet MS,et al.Fibrin-filled scaffolds for bone-tissue engineering:An in vivo study[J].J Biomed Mater Res,2004,71(1):162-171.
[36]丛明宇.载EPO-壳聚糖明胶水凝胶应用于兔上颌窦底提升术的研究[D].吉林:吉林大学,2015.
[37]Bokhari MA,Akay G,Zhang S,et al.The enhancement of osteoblast growth and differentiation in vitro on a peptide hydrogel-polyHIPE polymer hrbrid material[J].Biomaterials,2005,26(25):5198-5208.
[38]赵刚,李京玲,王丹,等.新型自组装水凝胶NBD/RADA16可促进前成骨细胞的分化[J].中国组织工程研究,2014,18(21):3329-3333.
[39]Antoine EE,Vlachos PP,Rylander MN.Review of collagen-I hydrogels for bioengineered tissue microenvironments:characterization of mechanics,structure,and transport[J].Tissue Eng,2014,20(6):638-696.
[40]Liu YC,Cai S,Xiao ZS,et al.Release of basic fibroblast growth factor from a crosslinked glycosaminoglycan hydrogel promotes wound healing[J].Wound Repair Regen,2007,15(2):245-251.
[41]Matsukuma D,Yamamoto K,Aoyagi T.Stimuli-responsive properties of N-isopropylacrylamide-based ultrathin hydrogel films prepared by photo-cross-linking[J].Langmuir,2006,22(13):5911-5915.
[42]Liu Y,Zhu MF,Liu XL,et al.High clay contentnanocomposite hydrogels with surprising mechanical strength and interesting deswelling kinetics[J].Polymer,2006,47(1):1-5.
[43]Grogan SP,Pauli C,Chen P,et al.In site tissue engineering using magnetically guided three-dimensional cell patterning[J].Tissue Eng,2012,18(7):496-506.
[44]Sapir Y,Cohen S,Friedman G,et al.The promotion of in vitro vessel-like organi-zation of endothelial cells in magnetically responsive alginate scaffolds[J].Biomaterials,2012,33(16):4100-4109.
[45]Paolo F,Sabrina B,Elisabetta R.Novel agmatine-containing poly(amidoamine)hydrogels as scaffolds for tissue engineering[J].Biomacromolecules,2005,6(4):2229-2235.
[2]Rufai0hah AJ,Seliktar D.Hydrogels for therapeutic cardiovascular angiogenesis[J].Adv Drug Deliv Rev,2016,15(96):31-39.
[3]Narayanan G,Vernekar VN,Kuyine EL,et al.Poly(lactic acid)-based biomaterials for orthopaedic regenerative engineering[J].Adv Drug Deliv Rev,2016,15(107):247-276.
[4]Kaqami H,Aqata H,Inoue M,et al.The use of bone marrow stromal cells(bone marrow-derived multipotent mesenchymal stromal cells)for alveolar bone tissue engineering:basic science to clinical translation[J].Tissue Enq Part B Rev,2014,20(3):229-232.
[5]韩浩,李利伟,秦文贞,等.ACF/PLGA骨组织工程复合支架的制备及其性能[J].材料科学与工程学报,2012,30(5):719
[6]杨维东,史培良,杨书勇,等.改善珊瑚表面成骨细胞黏附和增殖的研究[J].解放军医学杂志,2001,26(4):248-250.
[7]Zhang Y,Fu C,Li Y,et al.Synthesis of an injectable,self-healable and dual responsive hydrogel for drug delivery and 3D cell cultivation[J].Polym Chem,2017,8(3):537-544.
[8]Iwata T,Yamato M,Ishikawa I,et al.Tissue engineering in periodontal tissue[J].Anat Rec,2014,297(1):16-25.
[9]Chia HN,Wu BM.Recent advances in 3D printing of biomaterials[J].J Biol Eng,2015,9(1):4-18.
[10]Bose S,Roy M,Bandyopadhyay A.Recent advanced in bone tissue engineering scaffolds[J].Trends Biotechnol,2012,30(10):546-554.
[11]Velasco MA,Narvaez-Tovar CA,Grazon-Alvarado DA.Design,materials,and mechanobiology of biodegradable scaffolds for bone tissue engineering[J].Bio Med Res Inter,2015,2015(1-6):729076.
[12]Murphy CM,O Brien FJ,Little DG,et al.Cell-scaffold interactions in the bone tissue engineering triad[J].Eue Cells Mater,2013,26:120-132.
[13]Lou T,Wang X,Song G,et al.Structure and properties of PLLA/β-TCP nanocomposite scaffolds for bone tissue engineering[J].J Mater Sci Mater Med,2013,26(4):120-132.
[14]Liu Y,Lim J,Teoh SH.Review:Development of clinically relevant scaffolds for vascularised bone tissue engineering[J].Biotechnol Adv,2013,31(5):688-705.
[15]Pakulska MM,Vulic K,Tam RY,et al.Hybrid crosslinked methylcellulose hydrogel:A predictable and tunable platform for local drug delivery[J].Adv Mater,2015,27(34):5002-5008.
[16]Ni M,Zhang N,Xia W,et al.Dramatically promoted swelling of a hydrogel by pillar[6]arene-ferrocene complexation with multistimuli responsiveness[J].J Am Chem Soc,2016,138(20):6643-6649.
[17]Wenz A,Borchers K,Tovar GEM,et al.Bone matrix production in hydroxyapatite-modified hydrogels suitable for bone bioprinting[J].Biofabrication,2017,9(4):103-117.
[18]Tijore A,Irvine SA,Sariq U,et al.Contact guidence for cardiac tissue engineering using 3D bioprinted gelatin patterned hydrogel[J].Biofabrication,2017,12(13):228-267.
[19]Huang J,Jiang X.Injectable and degradable p H-responsive hydrogelvia spontaneous amino-yne click reaction[J].ACS Appl Mater Interfaces,2017,12(13):2-35.
[20]Chen W,Hao D,Hao W,et al.Hydrogel with ultra-fast selfhealing property both in air and underwater[J].ACS Appl Mater Interfaces,2017,11(13):18-45.
[21]Khorshidi S,Karkhaneh A.Hydrogel/fiber conductive scaffold for bone tissue engineering[J].J Biomed Mater Res A,2017,9(1):82-89.
[22]Gsib O,Duval JL,Goczkowski M,et al.Evaluation of fibrinbased interpenetrating polymer networks as potential biomaterials for tissue engineering[J].Nanomaterials,2017,7(12):436-457.
[23]Garza EB,Gomez S,Chayet A,et al.One-year safety and efficacy resultss of a hydrogel inlay to improve near vision in patients with emmetropic presbyopia[J].J Refract Surg,2013,29(3):166
[24]张磊,丁琪,夏扬,等.掺杂Fe3O4磁性纳米粒子壳聚糖凝胶的制备及其性质研究[J].东南大学学报,2012,31(5):567-571.
[25]Salick DA,Pochan DJ,Schneider JP.Design of an injectableβ-hairpin peptide hydrogel that kills methicillin-resistant staphylococcus aureus[J].Adv Mater,2010,21,21(41):4120-4123.
[26]Hoemann CD,Sun J,Legare A,et al.Tissue engineering of cartilage using an injectable and adhesive chitosan-based cell-delivery vehicle[J].Osteoarthritis Cartlage,2005,13(4):318-329.
[27]Hoemann CD,Chenite A,Sun J,et al.Cytocompatible gel formation of chitosan-glycerol phosphate solutions supplemented with hydroxyl ethyl cellulose is due to the presence of glyoxal[J].J Biomed Mater Res,2007,83(2):521-529.
[28]Chao PH,Yodmuang S,Wang X,et al.Silk hydrogel for cartliage tissue enginee-ring[J].J Biomed Mater Res,2010,95b(1):84-90.
[29]Yue K,Santiago GT,Alvarez MM,et al.Synthesis,properties,and biomedical applications of gelatin methacryloyl(Gel MA)hydrogels[J].Biomaterials,2015,73(12):8701-8707.
[30]Frasca S,Norol F,Visage CL,et al.Calcium-phosphate ceramics and polysaccharide-based hydrogel scaffolds combined with mesenchymal stem cell with mesenchymal stem cell differently support bone repair in rats[J].J Mate Sci Mater Med,2017,28(2):35-48.
[31]Zhao L,Wang LM,Detamore MS.Human umbilical cord mesenchymal stromal cell-based approach for osteochondral tissue engineering[J].J Tissue Eng Regen M,2011,5(9):712-721.
[32]何大为,金岩,骆凯,等.重组人骨形成蛋白-2/转化生长因子-β作用下陶瓷化骨粉/水凝胶与骨髓基质细胞修复自体颅骨缺损[J].中国临床康复,2005,9(46):339-341.
[33]Pappas NA,Hilt JZ,Khademhosseini A,et al.Hydrogels in biology and medicine:from molecular principles to bionanotechnology[J].Adv Mater,2006,18(11):1345-1366.
[34]Rowley JA,Madlambayan G,Mooney DJ.Alginate hydrogels as cynthetic extracellular matrix materials[J].Biomaterials,1999,20(1):45-53.
[35]Karp JM,Sarraf F,Shoichet MS,et al.Fibrin-filled scaffolds for bone-tissue engineering:An in vivo study[J].J Biomed Mater Res,2004,71(1):162-171.
[36]丛明宇.载EPO-壳聚糖明胶水凝胶应用于兔上颌窦底提升术的研究[D].吉林:吉林大学,2015.
[37]Bokhari MA,Akay G,Zhang S,et al.The enhancement of osteoblast growth and differentiation in vitro on a peptide hydrogel-polyHIPE polymer hrbrid material[J].Biomaterials,2005,26(25):5198-5208.
[38]赵刚,李京玲,王丹,等.新型自组装水凝胶NBD/RADA16可促进前成骨细胞的分化[J].中国组织工程研究,2014,18(21):3329-3333.
[39]Antoine EE,Vlachos PP,Rylander MN.Review of collagen-I hydrogels for bioengineered tissue microenvironments:characterization of mechanics,structure,and transport[J].Tissue Eng,2014,20(6):638-696.
[40]Liu YC,Cai S,Xiao ZS,et al.Release of basic fibroblast growth factor from a crosslinked glycosaminoglycan hydrogel promotes wound healing[J].Wound Repair Regen,2007,15(2):245-251.
[41]Matsukuma D,Yamamoto K,Aoyagi T.Stimuli-responsive properties of N-isopropylacrylamide-based ultrathin hydrogel films prepared by photo-cross-linking[J].Langmuir,2006,22(13):5911-5915.
[42]Liu Y,Zhu MF,Liu XL,et al.High clay contentnanocomposite hydrogels with surprising mechanical strength and interesting deswelling kinetics[J].Polymer,2006,47(1):1-5.
[43]Grogan SP,Pauli C,Chen P,et al.In site tissue engineering using magnetically guided three-dimensional cell patterning[J].Tissue Eng,2012,18(7):496-506.
[44]Sapir Y,Cohen S,Friedman G,et al.The promotion of in vitro vessel-like organi-zation of endothelial cells in magnetically responsive alginate scaffolds[J].Biomaterials,2012,33(16):4100-4109.
[45]Paolo F,Sabrina B,Elisabetta R.Novel agmatine-containing poly(amidoamine)hydrogels as scaffolds for tissue engineering[J].Biomacromolecules,2005,6(4):2229-2235.
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