摘要
玉米秸秆中纤维素含量丰富,具有较高的利用价值。但玉米秸秆存在降解困难的问题,筛选高效的玉米秸秆降解菌成为解决该问题的关键。通过富集培养、刚果红纤维素平板初筛和纤维素酶活力测定复筛的方法,从农田土壤中分离筛选出纤维素降解菌6株,其中菌株编号为L-4的秸秆降解菌的纤维素酶活力最高,为23.59 U/g,28℃条件下玉米秸秆15 d的降解率为23.32%。
Corn stover is rich in cellulose and has high utilization value. But the corn stalks are difficult to be degradated and screened efficiently. The corn stover degradation strains is the key to solving this problem. In this paper,6 strains of cellulose-degrading were isolated and screened from farmland soil by enrichment culture. With the congo red cellulose plate primary screening and cellulase activity re-screening methods, the 6 fibre-degrading bacteria were screened from the soil, and the straw-degrading strain with strain No. L-4 of the cellulase activity was the highest,which was 23.59 U/g, and the degradation rate of corn stalk for 15 days at 28℃ was 23.32%.
引文
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