降解玉米秸秆的毛头鬼伞菌株的初步筛选
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摘要
选取5株不同来源的毛头鬼伞(Coprinus comatus)菌株,通过平板筛选和酶活性检测等方法,比较其降解玉米秸秆木质纤维素的的能力,旨在筛选降解玉米秸秆能力较强的菌株。结果表明,1号、2号、4号菌株在平板筛选中的生长速度、菌丝密度、变色圈直径大于3号菌株和5号菌株;在液体培养的第10天,1号菌株产木聚糖酶、漆酶能力高于其他菌株,分别为42.36 U·mL~(-1)、10.39 U·mL~(-1)。
In this study, five strains of Coprinus comatus from different sources were selected, and the ability to degrade corn stalk lignocellulose was compared by using plate screeening and enzyme activity testing aiming at screening strains with strong ability to degrade corn stalk. The results showed that the growth rate, the hyphal density and discoloration diameter of No.l, No.2 and No.4 strain were larger compared with No.3 and No.5 strain by using the plate screening test. On the 10 th day of liquid culture, the xylanase and laccase production capacity of strain No.l were 42.36 U·mL~(-1) and 10.39 U·mL~(-1), respectively, which was higher than other strains.
In this study, five strains of Coprinus comatus from different sources were selected, and the ability to degrade corn stalk lignocellulose was compared by using plate screeening and enzyme activity testing aiming at screening strains with strong ability to degrade corn stalk. The results showed that the growth rate, the hyphal density and discoloration diameter of No.l, No.2 and No.4 strain were larger compared with No.3 and No.5 strain by using the plate screening test. On the 10 th day of liquid culture, the xylanase and laccase production capacity of strain No.l were 42.36 U·mL~(-1) and 10.39 U·mL~(-1), respectively, which was higher than other strains.
引文
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[14]杜海萍,宋瑞清,王钰祺.几种真菌产木质素降解酶的比较研究[J].林业科技,2006,31(4):20-24.
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[2] Ikehata K, Buchanan ID, Smith DW. Recent developments in the production of extracellular fungal peroxida[J]. Journal of Environmental Engineering&Science, 2011, 3(1):1-19.
[3]姜洁.玉米秸秆微生物预处理工艺筛选[D].重庆:西南大学,2010.
[4]何文.鸡腿菇营养价值高开发前案广阔[J].资源开发与利用,2000(4):11-12.
[5] Ward Q Hadar Y, DosorelzCG The Biodegradation of lignocellulose by white rot fungi[C]//Fungal biotechnology in agricultural, food,and environmental applications Boca Raton:CRC Press, 2004:393-407.
[6]刘朝贵,邵坤,聂和平,等.不同培养料对鸡腿菇胞外酶活性影响的研究[J].西南师范大学学报,2008, 33(1):26.
[7]谢君,任路,张义正,等.侧耳菌产生木质纤维素酶及其降解植物生物质的研究[J].兰州大学学报:自然科学版,2002, 38(2):121-126.
[8]宋安东,周利霞,胡渝,等.产木质素降解酶类香菇菌株的筛选[J].河南农业大学学报,2006, 4(3):307-310.
[9]郭夏丽,程小平,杨小丽,等.高效玉米秸秆降解菌复合系的构建[J].中国农学通报,2010, 26(7):261-266.
[10]宋安东,吕洋,尤晓燕,等.几种食用菌产木质纤维素降解酶酶类研究[J].中国食用菌,2005, 24(2):39-41.
[11] Pointing. Feasibility of bioremediation by white-rot fungi[J].Lied Microbiology Biotechnology, 2007, 57(2):20-33.
[12]牛文娟.主要农作物秸秆组成成分和能源利用潜力[D].北京:中国农业大学,2015.
[13]王小娟,李培培,袁旭峰.4组常温分解小麦秸秆复合菌系的比较研究[J].中国农业大学学报,2011, 16(1):24-29.
[14]杜海萍,宋瑞清,王钰祺.几种真菌产木质素降解酶的比较研究[J].林业科技,2006,31(4):20-24.
[15]孙江慧.几株食用菌对秸秆木质纤维素降解能力的研究[D].南京:南京农业大学,2012.
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