燕麦萌发期抗旱指标体系构建及综合评价
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摘要
为探究不同燕麦品种间的抗旱性强弱,构建燕麦品种抗旱评价体系,以6种燕麦品种为试验材料,分别测定蒸馏水(对照,CK)、-0.2、-0.4、-0.8及-1.2 MPa聚乙二醇(PEG-6000)渗透胁迫下不同燕麦品种发芽势、发芽率、发芽指数等7项指标,分析不同干旱胁迫对燕麦种子萌发的影响;以鉴定指标相对值作为抗旱评价指标,在主成分分析的基础上利用GGE-biplot双标图,构建供试燕麦品种抗旱性的评价指标体系,并结合加权隶属函数法综合评价各燕麦品种的抗旱性能。结果表明,-0.2 MPa和-0.4 MPa PEG-6000处理对种子萌发和幼苗生长具有促进作用,其中不同燕麦品种在-0.4 MPa胁迫下相对发芽率、相对活力指数均显著高于CK和-1.2 MPa胁迫组(P<0.05)。利用主成分分析与GGE-biplot双标图将7项评价指标转换为3个综合指标(Z1、Z2、Z3),构建出不同燕麦品种萌发期抗旱性评价体系。在Z1、Z2、Z3权重的基础上,结合加权隶属函数法评价出供试材料种子萌发期抗旱性强弱为青海燕麦>甜燕1号燕麦>奥塔燕麦>ESK燕麦>普兰顿燕麦>干旱燕麦。本研究结果为燕麦抗旱性评价、抗旱品种选育及其机理研究提供了理论依据。
In order to explore the drought resistance ability and construct a drought resistance evaluation system among different oat cultivars,six oat varieties were used as experimental materials the impact of drought stress( CK,-0. 2,-0. 4,-0. 8 and~(-1). 2 MPa polyethylene glycol) on oat seed germination of six oat cultivars were evaluated. Seven indexes of seed germination characteristics were tested including germination potential,germination rate,germination index,and etc. Based on the principal component analysis using GGE-biplot,the evaluation index system of drought resistance of tested oat varieties was constructed by using the relative value of index as drought resistance evaluation index,and the weighting membership function method was used to comprehensively evaluate the drought resistance. The results showed that: 1) The seed germination and seedling growth were accelerated by low concentration polyethylene glycol( PEG-6000) under-0. 2 MPa and-0. 4 MPa. The relative germination rate and relative vigour index of different oat varieties under-0. 4 MPa stress were significantly higher than the treatments of CK and~(-1). 2 MPa( P <0. 05). 2) Drought resistance index system among different oats was constructed by combining principal component analysis with GGE-biplotto shift the seven seed germination indexes into three comprehensive indexes of Z1,Z2 and Z3. 3) On the basis of these three comprehensive indexes weight,the drought resistance of the tested materials were evaluated by the weighted membership function method. The results showed that the drought resistance of the tested materials were as follows: Oat Qinghai > Oat Sweet yan no. 1 > Oat Ota > Oat ESK > Oat Phnum > Oat Drought.This study provided a theoretical basis for evaluation of drought resistance,the research of breeding and mechanism of drought resistance varieties.
In order to explore the drought resistance ability and construct a drought resistance evaluation system among different oat cultivars,six oat varieties were used as experimental materials the impact of drought stress( CK,-0. 2,-0. 4,-0. 8 and~(-1). 2 MPa polyethylene glycol) on oat seed germination of six oat cultivars were evaluated. Seven indexes of seed germination characteristics were tested including germination potential,germination rate,germination index,and etc. Based on the principal component analysis using GGE-biplot,the evaluation index system of drought resistance of tested oat varieties was constructed by using the relative value of index as drought resistance evaluation index,and the weighting membership function method was used to comprehensively evaluate the drought resistance. The results showed that: 1) The seed germination and seedling growth were accelerated by low concentration polyethylene glycol( PEG-6000) under-0. 2 MPa and-0. 4 MPa. The relative germination rate and relative vigour index of different oat varieties under-0. 4 MPa stress were significantly higher than the treatments of CK and~(-1). 2 MPa( P <0. 05). 2) Drought resistance index system among different oats was constructed by combining principal component analysis with GGE-biplotto shift the seven seed germination indexes into three comprehensive indexes of Z1,Z2 and Z3. 3) On the basis of these three comprehensive indexes weight,the drought resistance of the tested materials were evaluated by the weighted membership function method. The results showed that the drought resistance of the tested materials were as follows: Oat Qinghai > Oat Sweet yan no. 1 > Oat Ota > Oat ESK > Oat Phnum > Oat Drought.This study provided a theoretical basis for evaluation of drought resistance,the research of breeding and mechanism of drought resistance varieties.
引文
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[5]张娜,赵宝平,张艳丽,任鹏,刘景辉,王莹.干旱胁迫下燕麦叶片抗氧化酶活性等生理特性变化及抗旱性比较[J].干旱地区农业研究,2013,31(1):166-171
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[7]陈雅君,刘慧民,张雪岩,梁敏,王超.水分胁迫对草地早熟禾生长及水分生理指标的影响[J].中国草地学报,2009,31(2):59-63
[8]Halim A,Normaniza O.The effects of plant density of Melastoma malabathricum on the erosion rate of slope soil at different slope orientations[J].International Journal of Sediment Research,2015,30(2):131-141
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[11]柯贞进,尹美强,温银元,黄明镜,黄学芳,郭平毅,王玉国,原向阳.干旱胁迫下聚丙烯酰胺浸种对谷子种子萌发及幼苗期抗旱性的影响[J].核农学报,2015,29(3):563-570
[12]裴帅帅,尹美强,温银元,黄明镜,张彬,郭平毅,王玉国,原向阳.不同品种谷子种子萌发期对干旱胁迫的生理响应及其抗旱性评价[J].核农学报,2014,28(10):1897-1904
[13]李娜,罗俊杰,张仁陟,刘新星.持续模拟干旱胁迫对燕麦萌发特性影响及品种抗旱性评价研究[J].核农学报,2016,30(2):379-387
[14]刘光辉,陈全家,吴鹏昊,曲延英,高文伟,杨军善,杜荣光.棉花花铃期抗旱性综合评价及指标筛选[J].植物遗传资源学报,2016,17(1):53-62
[15]罗俊杰,欧巧明,叶春雷,王方,王镛臻.主要胡麻品种抗旱相关指标分析及综合评价[J].核农学报,2014,28(11):2115-2125
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[17]陈新,宋高原,张宗文,吴斌.PEG-6000胁迫下裸燕麦萌发期抗旱性鉴定与评价[J].植物遗传资源学报,2014,15(6):1188-1195
[18]朱学海,宋燕春,赵治海,石云素,刘颖慧,黎裕,王天宇.用渗透剂胁迫鉴定谷子芽期耐旱性的方法研究[J].植物遗传资源报,2008,9(1):62-67
[19]徐振朋,宛涛,蔡萍,张雅荣,于静,孟超.PEG模拟干旱胁迫对罗布麻种子萌发及生理特性的影响[J].中国草地学报,2015,37(5):75-80
[20]姚玉波.不同品种亚麻种子萌发期抗旱性鉴定[J].核农学报,2015,29(10):2033-2039
[21]贾寿山,朱俊刚,王曙光,史雨刚,孙黛珍.山西小麦地方品种萌发期的抗旱性[J].华北农学报,2011,26(2):213-217
[22]鞠乐,齐军仓,贺雪,王丹,侯忠庆,付强,熊显鹏.大麦种子萌发期对渗透胁迫的响应及抗旱性鉴定指标的筛选[J].干旱地区农业研究,2013,31(1):172-176
[23]李志博,魏亦农.北疆主栽棉花种子对渗透胁迫的响应及其萌发力差异评价[J].种子,2010,29(7):1-4
[24]谢小玉,张霞,张兵.油菜苗期抗旱性评价及抗旱相关指标变化分析[J].中国农业科学,2013,46(3):476-485
[25]周桂莲,杨慧霞.小麦抗旱性鉴定的生理生化指标及其分析评价[J].干旱地区农业研究,1996,14(2):65-71
[26]景蕊莲,昌小平.用渗透胁迫鉴定小麦种子萌发期抗旱性的方法分析[J].植物遗传资源学报,2003,4(4):292-296
[27]李志博,林海荣,魏亦农,郗忠玲,喻娟.北疆主栽棉花抗旱性生育期差异评价及鉴定体系的初步建立[J].干旱地区农业研究,2011,29(3):84-90
[28]杨进文,朱俊刚,王曙光,孙黛珍,史雨刚,陈卫国.用GGE双标图及隶属函数综合分析山西小麦地方品种抗旱性[J].应用生态学报,2013,24(4):1031-1038
[29]王尊欣.31个甘蔗品种田间综合评价及优良品种的抗旱性分析[D].海口:海南大学,2014
[30]李贵全,张海燕,季兰,赵二开,刘建兵,李玲,张家蓉.不同大豆品种抗旱性综合评价[J].应用生态学报,2012,17(12):2408-2412
[2]牛瑞明,王燕,吴桂丽,高明照.裸燕麦种子萌发对模拟干旱胁迫的响应及其耐旱性综合评价[J].麦类植物学报,2011,31(4):753-756
[3]Subrahmanyam D,Subash N,Haris A,Sikka A K.Influence of water stress on leaf photosynthetic characteristics in wheat cultivars differing in their susceptibility to drought[J].Photosynthetica,2015,44(1):125-129
[4]Suriyan C,Chalermpol K.Proline accumulation photosynthetic abilities and growth characters of sugarcane(Saccharum officinarum L.)plantlets in response to iso-osmotic salt and water-deficit stress[J].Agricultural Sciences in China,2009,8(1):51-58
[5]张娜,赵宝平,张艳丽,任鹏,刘景辉,王莹.干旱胁迫下燕麦叶片抗氧化酶活性等生理特性变化及抗旱性比较[J].干旱地区农业研究,2013,31(1):166-171
[6]程建峰,潘晓云,刘宜柏,戴廷波,曹卫星.水稻抗旱性鉴定的形态指标[J].生态学报.2015,25(11):325-333
[7]陈雅君,刘慧民,张雪岩,梁敏,王超.水分胁迫对草地早熟禾生长及水分生理指标的影响[J].中国草地学报,2009,31(2):59-63
[8]Halim A,Normaniza O.The effects of plant density of Melastoma malabathricum on the erosion rate of slope soil at different slope orientations[J].International Journal of Sediment Research,2015,30(2):131-141
[9]Juan J,Camacho-Cristóbal,Rexach J.Boron in plants:deficiency and toxicity[J].Journal of Integrative Plant Biology,2008,50(10):1247-1255
[10]罗俊杰,欧巧明,叶春雷,王方,王镛臻,陈玉梁.重要胡麻栽培品种的抗旱性综合评价及指标筛选[J].作物学报,2014,40(7):1259-1273
[11]柯贞进,尹美强,温银元,黄明镜,黄学芳,郭平毅,王玉国,原向阳.干旱胁迫下聚丙烯酰胺浸种对谷子种子萌发及幼苗期抗旱性的影响[J].核农学报,2015,29(3):563-570
[12]裴帅帅,尹美强,温银元,黄明镜,张彬,郭平毅,王玉国,原向阳.不同品种谷子种子萌发期对干旱胁迫的生理响应及其抗旱性评价[J].核农学报,2014,28(10):1897-1904
[13]李娜,罗俊杰,张仁陟,刘新星.持续模拟干旱胁迫对燕麦萌发特性影响及品种抗旱性评价研究[J].核农学报,2016,30(2):379-387
[14]刘光辉,陈全家,吴鹏昊,曲延英,高文伟,杨军善,杜荣光.棉花花铃期抗旱性综合评价及指标筛选[J].植物遗传资源学报,2016,17(1):53-62
[15]罗俊杰,欧巧明,叶春雷,王方,王镛臻.主要胡麻品种抗旱相关指标分析及综合评价[J].核农学报,2014,28(11):2115-2125
[16]中国种子总站.GB/T 3543.4-1995农作物种子检验规程发芽试验[S].北京:中国标准出版社,1995
[17]陈新,宋高原,张宗文,吴斌.PEG-6000胁迫下裸燕麦萌发期抗旱性鉴定与评价[J].植物遗传资源学报,2014,15(6):1188-1195
[18]朱学海,宋燕春,赵治海,石云素,刘颖慧,黎裕,王天宇.用渗透剂胁迫鉴定谷子芽期耐旱性的方法研究[J].植物遗传资源报,2008,9(1):62-67
[19]徐振朋,宛涛,蔡萍,张雅荣,于静,孟超.PEG模拟干旱胁迫对罗布麻种子萌发及生理特性的影响[J].中国草地学报,2015,37(5):75-80
[20]姚玉波.不同品种亚麻种子萌发期抗旱性鉴定[J].核农学报,2015,29(10):2033-2039
[21]贾寿山,朱俊刚,王曙光,史雨刚,孙黛珍.山西小麦地方品种萌发期的抗旱性[J].华北农学报,2011,26(2):213-217
[22]鞠乐,齐军仓,贺雪,王丹,侯忠庆,付强,熊显鹏.大麦种子萌发期对渗透胁迫的响应及抗旱性鉴定指标的筛选[J].干旱地区农业研究,2013,31(1):172-176
[23]李志博,魏亦农.北疆主栽棉花种子对渗透胁迫的响应及其萌发力差异评价[J].种子,2010,29(7):1-4
[24]谢小玉,张霞,张兵.油菜苗期抗旱性评价及抗旱相关指标变化分析[J].中国农业科学,2013,46(3):476-485
[25]周桂莲,杨慧霞.小麦抗旱性鉴定的生理生化指标及其分析评价[J].干旱地区农业研究,1996,14(2):65-71
[26]景蕊莲,昌小平.用渗透胁迫鉴定小麦种子萌发期抗旱性的方法分析[J].植物遗传资源学报,2003,4(4):292-296
[27]李志博,林海荣,魏亦农,郗忠玲,喻娟.北疆主栽棉花抗旱性生育期差异评价及鉴定体系的初步建立[J].干旱地区农业研究,2011,29(3):84-90
[28]杨进文,朱俊刚,王曙光,孙黛珍,史雨刚,陈卫国.用GGE双标图及隶属函数综合分析山西小麦地方品种抗旱性[J].应用生态学报,2013,24(4):1031-1038
[29]王尊欣.31个甘蔗品种田间综合评价及优良品种的抗旱性分析[D].海口:海南大学,2014
[30]李贵全,张海燕,季兰,赵二开,刘建兵,李玲,张家蓉.不同大豆品种抗旱性综合评价[J].应用生态学报,2012,17(12):2408-2412