不同形态锌化合物对己烯雌酚诱导的氧化性损伤的保护作用
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摘要
目的研究氯化锌(ZnCl_(2 )),络合锌(Zn-EDTA)和葡萄糖酸锌(Zn-GLU)对己烯雌酚诱导的氧化性损伤和白细胞介素改变的作用。方法将100只昆明种雌鼠随机分为空白对照组、染毒组,ZnCl_2组,Zn-EDTA组,Zn-GLU组。除空白对照组外,其它组给予己烯雌酚,各补锌组(ZnCl_2组、Zn-EDTA组和Zn-GLU组)再给予相应的药物。脱颈处死小鼠,测定血清中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量以及白细胞介素-2(IL-2)和白细胞介素-10(IL-10)的含量。结果染毒组血清中IL-10、SOD和GSH-Px活性显著低于空白对照组,血清中IL-2、MDA的含量显著高于空白对照组(P<0.05);各补锌组小鼠血清中SOD和GSH-Px活性显著高于染毒组(P<0.05),MDA和IL-2的含量显著低于染毒组(P<0.05)。结论锌对己烯雌酚诱导的氧化性损伤和白细胞介素改变具有一定的保护作用。
Objective To study the effects of zinc chloride(ZnCl_2),disodium zinc ethylenediamine tetraacetate(Zn-EDTA),and zinc gluconate(Zn-GLU)on the oxidative damage and level changes of interleukins(ILs)caused by diethylstilbestrol.Methods 100 female Kunming mice were randomly divided into 5 groups:control group,poisoned group,ZnCl_2 group,Zn-EDTA group,and Zn-GLU group.Except for the control group,the other groups were given diethylstilbestrol,and the zinc supplementation group(ZnCl_2 group,Zn-EDTA group and Zn-GLU group)were given the corresponding drugs.All the mice were sacrificed by cervical dislocation,and the serum levels of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),malondialdehyde(MDA),interleukin-2(IL-2)and interleukin-10(IL-10)were detected.Results The levels of serum IL-10,SOD and GSH-Px of the poisoned group were significantly lower than that of the control group,and the contents of serum IL-2 and MDA were significantly higher than that of the control group(P<0.05).Compared with the poisoned group,the activity of SOD and GSH-Px in the serum of each supplemental zinc group was significantly higher(P<0.05),and the contents of MDA and IL-2 were significantly lower(P<0.05).Conclusion Zinc has a protective effect against oxidative damage and level changes of ILs caused by diethylstilbestrol.
Objective To study the effects of zinc chloride(ZnCl_2),disodium zinc ethylenediamine tetraacetate(Zn-EDTA),and zinc gluconate(Zn-GLU)on the oxidative damage and level changes of interleukins(ILs)caused by diethylstilbestrol.Methods 100 female Kunming mice were randomly divided into 5 groups:control group,poisoned group,ZnCl_2 group,Zn-EDTA group,and Zn-GLU group.Except for the control group,the other groups were given diethylstilbestrol,and the zinc supplementation group(ZnCl_2 group,Zn-EDTA group and Zn-GLU group)were given the corresponding drugs.All the mice were sacrificed by cervical dislocation,and the serum levels of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),malondialdehyde(MDA),interleukin-2(IL-2)and interleukin-10(IL-10)were detected.Results The levels of serum IL-10,SOD and GSH-Px of the poisoned group were significantly lower than that of the control group,and the contents of serum IL-2 and MDA were significantly higher than that of the control group(P<0.05).Compared with the poisoned group,the activity of SOD and GSH-Px in the serum of each supplemental zinc group was significantly higher(P<0.05),and the contents of MDA and IL-2 were significantly lower(P<0.05).Conclusion Zinc has a protective effect against oxidative damage and level changes of ILs caused by diethylstilbestrol.
引文
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[18] 其力根,阿拉坦敖其尔,武飞,等.黄芪多糖对己烯雌酚诱导的小鼠睾丸损伤保护作用研究[J].动物医学进展,2013,34(6):47-50.
[19] Lke S.Induction of interleukin-10 producing dendritic cells as a tool to suppress allergen-specific T helper 2 responses[J].Frontiers in Immunology,2018,9:455.
[20] Komai T,Inoue M,Okamura T,et al.Transforming growth factor-β and interleukin-10 synergistically regulate humoral immunity via modulating metabolic signals[J].Frontiers in Immunology,2018,9:1364.
[2] 师博颖,王智源,刘俊杰,等.长江江苏段饮用水源地3种雌激素污染特征[J].环境科学学报,2018,38(3):875-883.
[3] 李军延,乔佩环,张林媛,等.己烯雌酚诱导的氧化应激对青春期大鼠睾丸类固醇合成的影响[J].中国比较医学杂志,2014,24(6):1-6.
[4] 杜娟,潘红艳,宫智勇.己烯雌酚对生殖系统的影响及机制的研究进展[J].中国药理学与毒理学杂志,2012,26(4):588-590.
[5] Habas K,Brinkworth MH,Anderson D.Diethylstilbestrol induces oxidative DNA damage,resulting in apoptosis of spermatogonial stem cells in vitro[J].Toxicology,2017,382:117-121.
[6] Shaliutina O,Shaliutina-Kole?ová A,Lebeda I,et al.The in vitro effect of nonylphenol,propranolol,and diethylstilbestrol on quality parameters and oxidative stress in sterlet(Acipenser ruthenus)spermatozoa[J].Toxicology in Vitro,2017,43:9-15.
[7] 吕廷廷.己烯雌酚抑制刀豆蛋白A诱导的小鼠急性自身免疫性肝炎[D].广州:南方医科大学,2013:1-65.
[8] 任妮丽,陈萍.白介素6在雌激素促进小鼠肺腺癌进展中的作用及其机制[J].肿瘤防治研究,2016,43(4):253-257.
[9] 朱泓,杨祖菁,惠宁,等.孕期补锌对母胎汞毒性的拮抗作用及其抗氧化损伤机制研究[J].上海交通大学学报(医学版),2014,34(2):193-196,200.
[10] 李家丽,杨汀,刘海荣,等.硫酸锌诱导金属硫蛋白2表达对脓毒症小鼠脏器损伤的保护作用[J].成都医学院学报,2017,12(2):133-138.
[11] 其力根,额尔登其木格,武飞,等.葛根素缓解己烯雌酚诱导的雄性小鼠生殖损伤研究[J].黑龙江畜牧兽医,2013(21):148-150.
[12] 杜娟,潘红艳,林利美,等.黄酮和维生素E对己烯雌酚所致雄性小鼠肝脏氧化损伤的保护作用[J].中国畜牧兽医,2013,40(4):149-153.
[13] 付军.锌及络合锌抗银屑病作用实验研究[D].济南:山东大学,2007:1-57.
[14] Baek JO,Byamba D,Wu WH,et al.Assessment of an imiquimod-induced psoriatic mouse model in relation to oxidative stress[J].Archives of Dermatological Research,2012,304(9):699-706.
[15] 张彩利,刘玉姣,王安忠,等.枸杞多糖对己烯雌酚生殖损伤仓鼠抗氧化能力及生殖激素水平的影响[J].中国兽医学报,2014,34(8):1364-1368,1372.
[16] 胥可,贾强,张娟,等.多菌灵诱导小鼠睾丸支持细胞凋亡及周期阻滞的研究[J].现代预防医学,2018,45(4):700-703,736.
[17] 储兰兰,许猛,王妍,等.小鼠急性酒精性肝损伤模型氧化损伤指标的动态监测[J].现代预防医学,2017,44(14):2605-2608,2622.
[18] 其力根,阿拉坦敖其尔,武飞,等.黄芪多糖对己烯雌酚诱导的小鼠睾丸损伤保护作用研究[J].动物医学进展,2013,34(6):47-50.
[19] Lke S.Induction of interleukin-10 producing dendritic cells as a tool to suppress allergen-specific T helper 2 responses[J].Frontiers in Immunology,2018,9:455.
[20] Komai T,Inoue M,Okamura T,et al.Transforming growth factor-β and interleukin-10 synergistically regulate humoral immunity via modulating metabolic signals[J].Frontiers in Immunology,2018,9:1364.