柴胡愈伤组织提取物抗肝癌效用研究
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摘要
目的:对中药柴胡的愈伤组织进行培养,并对其抗肿瘤活性进行初步研究。方法:提取柴胡愈伤组织活性成分(BSW-ws),对培养的肝癌细胞SMMC-7221给药,给药浓度分别为:10,20,30,40,60,80,100μg·mL~(-1);再用MTT法检测BSW-ws的细胞毒活性,并经流式细胞仪检测BSW-ws对细胞凋亡的影响。结果:(1)MTT法测BSW-ws的细胞毒活性结果显示,当给药浓度为60μg·mL~(-1)时达到IC50值;(2)流式细胞仪检测结果表明,BSW-ws能够促进肝癌细胞SMMC-7221的凋亡。结论:柴胡愈伤组织提取物BSW-ws通过促进癌细胞凋亡的形式抑制肝癌细胞SMMC-7221的增殖。
Objective:To culture the callus of Chinese traditional medicine Radix Bupleuri and start a preliminary research on its anti-tumor activity.Methods:The active constituents of Bupleurum callus(BSW-ws)were extracted and administered to cultured liver cancer cells SMMC-7221 at concentrations of 10,20,30,40,60,80,100μg·mL~(-1).Then,the cytotoxic activity of BSW-ws was detected by MTT assay,and the effect of BSW-ws on apoptosis was detected by flow cytometry.Results:(1)The cytotoxic activity of BSW-ws by MTT assay showed that IC50 value was reached when the concentration was 60μg·mL~(-1).(2)Flow cytometry showed that BSW-ws could promote cell apoptosis of SMMC-7221.Conclusion:Bupleurum callus extract,BSW-ws,could inhibit the growth of SMMC-7221 cells by promoting the apoptosis of hepatoma cells.
Objective:To culture the callus of Chinese traditional medicine Radix Bupleuri and start a preliminary research on its anti-tumor activity.Methods:The active constituents of Bupleurum callus(BSW-ws)were extracted and administered to cultured liver cancer cells SMMC-7221 at concentrations of 10,20,30,40,60,80,100μg·mL~(-1).Then,the cytotoxic activity of BSW-ws was detected by MTT assay,and the effect of BSW-ws on apoptosis was detected by flow cytometry.Results:(1)The cytotoxic activity of BSW-ws by MTT assay showed that IC50 value was reached when the concentration was 60μg·mL~(-1).(2)Flow cytometry showed that BSW-ws could promote cell apoptosis of SMMC-7221.Conclusion:Bupleurum callus extract,BSW-ws,could inhibit the growth of SMMC-7221 cells by promoting the apoptosis of hepatoma cells.
引文
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[2]程玉鹏,李弘琨,马爱萍,等.柴胡黄酮类抗氧化作用机制最新研究进展[J].化学工程师,2017,31(7):47-48;51.
[3]S kmen M,Akram Khan M.The antioxidant activity of some curcuminoids and chalcones.Inflammopharmacology.2016,24(2-3):81-6.
[4]Chu,J.and Guo,C.Design and Discovery of Some Novel Chalcones as Antioxidant and Anti Inflammatory Agents via Attenuating NFκB.Arch.Pharm.Chem.Life Sci,2016,349:63-70.
[5]Tong H1,Tian D,He Z,et al.Polysaccharides from Bupleurum chinense impact the recruitment and migration of neutrophils by blocking f MLP chemoattractant receptor-mediated functions.Carbohydr Polym,2013,92(2):1071-7.
[6]李廷利,都晓伟,赵景和,等.南北柴胡药理作用的比较研究——解热和保肝作用的实验研究[J].中医药学报,1992,(3):34-37.
[7]李红霞.富马酸二甲酯抑制和保护恶性黑色素瘤细胞的双重作用以及干预机制的研究[D].大连大学,2016.