冬小麦抗寒性鉴定的低温处理方式和鉴定指标的研究
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摘要
为了筛选冬小麦抗寒性鉴定的低温处理方式和鉴定指标,以冬小麦强抗寒品种东农冬麦1号、弱抗寒品种济麦22和不抗寒品种中国春为试验材料,设室内快速低温、室内缓慢低温和田间种植三种处理,测定和分析了不同处理下小麦叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)活性及抗坏血酸(ASA)、谷胱甘肽(GSH)、丙二醛(MDA)、过氧化氢(H_2O_2)含量。结果表明,与室内缓慢低温处理相比,室内快速低温处理下小麦各项指标较接近田间种植处理,且与田间种植处理相关性较显著。低温下抗寒品种的H_2O_2及MDA含量显著低于弱抗寒品种和不抗寒品种,SOD活性和ASA含量与H_2O_2含量呈极显著负相关。因此,室内快速低温处理结合SOD活性及ASA、H_2O_2和MDA含量分析可以进行小麦品种抗寒性鉴定。
In order to screen the low temperature treatment methods and identification indices of cold resistance screening of winter wheat, three varieties:Dongnongdongmai 1(high cold-resistant), Jimai 22(weak cold-resistant) and China Spring(non-cold-resistant) were selected as materials and three treatments were set up, i.e. indoor rapid low temperature treatment, indoor slow low temperature treatment and field planting. Content of malondialdehyde(MDA), hydrogen peroxide(H_2O_2) and reactive oxygen species scavenging substances(SOD, POD, APX, ASA, GSH, CAT) were detected. The results showed that the physiological indices of indoor rapid low temperature treatment were closer to the field, and the correlation with the field planting was significant. The content of H_2O_2 and MDA in high cold-resistant variety was significantly lower than that in weak-resistant and non-cold-resistant variety. The contents of SOD and ASA were significantly negatively correlated with H_2O_2. Therefore, indoor rapid cold treatment, combined with SOD, ASA, H_2O_2 and MDA detection can be used to identify the cold resistance of wheat.
In order to screen the low temperature treatment methods and identification indices of cold resistance screening of winter wheat, three varieties:Dongnongdongmai 1(high cold-resistant), Jimai 22(weak cold-resistant) and China Spring(non-cold-resistant) were selected as materials and three treatments were set up, i.e. indoor rapid low temperature treatment, indoor slow low temperature treatment and field planting. Content of malondialdehyde(MDA), hydrogen peroxide(H_2O_2) and reactive oxygen species scavenging substances(SOD, POD, APX, ASA, GSH, CAT) were detected. The results showed that the physiological indices of indoor rapid low temperature treatment were closer to the field, and the correlation with the field planting was significant. The content of H_2O_2 and MDA in high cold-resistant variety was significantly lower than that in weak-resistant and non-cold-resistant variety. The contents of SOD and ASA were significantly negatively correlated with H_2O_2. Therefore, indoor rapid cold treatment, combined with SOD, ASA, H_2O_2 and MDA detection can be used to identify the cold resistance of wheat.
引文
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[28]INZé D.Extraction and determination of ascorbate and dehydroascorbate from plant tissue [J].Analytical Biochemistry,1995,225(1):165.
[29]MA F,CHENG L.The sun-exposed peel of apple fruit has higher xanthophyll cycle-dependent thermal dissipation and antioxidants of the ascorbate–glutathione pathway than the shaded peel [J].Plant Science,2003,165(4):821.
[30]ESIM N,ATICI O,MUTLU S.Effects of exogenous nitric oxide in wheat seedlings under chilling stress [J].Toxicology & Industrial Health,2012,30(3):270.
[31]ZHANG B,JIA D,GAO Z,et al.Physiological responses to low temperature in spring and winter wheat varieties [J].Journal of the Science of Food & Agriculture,2016,96(6):1969.
[32]ZHANG D,REN L,CHEN G Q,et al.ROS-induced oxidative stress and apoptosis-like event directly affect the cell viability of cry preserved embryogenic callus in Agapanthus praecox [J].Plant Cell Reports,2015,34(9):1505.
[33]SMIRNOFF N.The role of active oxygen in the response of plants to water deficit and desiccation [J].New Phytologist,1993,125(1):31.
[34]GILL S S,TUTEJAN.Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants [J].Plant Physiology & Biochemistry,2010,48(12):918.
[35]YOSHIMURA K.Expression of spinach ascorbate peroxidase isoenzymes in response to oxidative stresses [J].Plant Physiology,2000,123(1):225.
[36]MILLER A F.Superoxide dismutases:Ancient enzymes and new insights [J].Febs Letters,2012,586(5):587.
[37]DGRAHAM A,PATTERSON B D.Respo^nses of plants to low,nonfreezing temperatures:proteins,metabolism,and acclimation [J].Annu.Rev.Plant Physiol,2003,33(1):349.
[38]XU J.Comparative physiological and proteomic response to abrupt low temperature stress between two winter wheat cultivars differing in low temperature tolerance [J].Plant Biology,2013,15(2):297.
[39]WANG Y L,KANG J.Effects of low temperature stress on physiological and biochemical characteristics of winter wheat in seedling and jointing stages [J].Journal of Henan Agricultural Sciences,2004.
[2]JANMOHAMMADI M,ZOLLA L,RINALDUCCI S.Low temperature tolerance in plants:Changes at the protein level [J].Phytochemistry,2015,117:77.
[3]王晓楠,付连双,李卓夫,等.低温驯化及封冻阶段不同冬小麦品种叶绿素荧光参数的比较[J].麦类作物学报,2009,29(1):83.WANG X N,FU L S,LI Z F,et al.Comparison of chlorophyll fluorescence parameters between two winter wheat cultivars during cold acclimation and frozen period [J].Journal of Triticeae Crops,2009,29(1):87.
[4]RAKEI A,MAALIAMIRI R,ZEINALI H,et al.DNA methylation and physio-biochemical analysis of chickpea in response to cold stress [J].Protoplasma,2016,253(1):64.
[5]SANGHERA G S,WANI S H,WASIM H,et al.Engineering cold stress tolerance in crop plants [J].Current Genomics,2011,12(1):33.
[6]YU G,HOU W,DU X,et al.Identification of wheat non-specific lipid transfer proteins involved in chilling tolerance [J].Plant Cell Reports,2014,33(10):1757.
[7]NEJADSADEGHI L,MAALI-AMIRI R,ZEINALIH,et al.Membrane fatty acid compositions and cold-induced responses in tetraploid and hexaploid wheats [J].Molecular Biology Reports,2015,42(2):366.
[8]AN F,LI G,LI Q X,et al.The comparatively proteomic analysis in response to cold stress in cassava plant lets [J].Plant Molecular Biology Reporter,2016,34(6):1098.
[9]SUN Y,FU L,CHEN L,et al.Characterization of two winter wheat varietie’ responses to freezing in a frigid region of the People's Republic of China [J].Canadian Journal of Plant Science,2016,97(5):814.
[10]LIU Y,JIANG H,ZHAO Z,et al.Nitric oxide synthase like activity-dependent nitric oxide production protects against chilling-induced oxidative damage in Chorispora bungeana suspension cultured cells [J].Plant Physiology & Biochemistry,2010,48(12):938.
[11]LI X,JIAN C,LIU F,et al.Cold priming drives the sub-cellular antioxidant systems to protect photosynthetic electron transport against subsequent low temperature stress in winter wheat [J].Plant Physiology & Biochemistry,2014,82(3):40.
[12]THAKUR P,NAYYAR H.Facing the cold stress by plants in the changing environment:Sensing,signaling,and defending mechanisms [C]//Plant Acclimation to Environmental Stress.Springer New York,2013:49.
[13]MUTLU S,?KKE?ATICI,ESIMN,et al.Essential oils of catmint(Nepeta meyeri,Benth.) induce oxidative stress in early seedlings of various weed species [J].Acta Physiologiae Plantarum,2011,33(3):947.
[14]陈露,李卓夫,王晓楠,等.低温下冬小麦SOD及APX相关基因的表达分析[J].麦类作物学报,2016.36(8):985.CHEN L,LI Z F,WANG X N,et al.Expression of genes related to SOD and APX enzumes in winter wheat at low temperature [J].Journal of Triticeae Crops,2016,36(8):985.
[15]林郑和,钟秋生,游小妹,等.低温胁迫对茶树抗氧化酶活性的影响[J].茶叶科学,2018,38(4):369.LIN Z H,ZHONG Q S,YOU X M,et al.Antioxidant enzyme activity of tea plant(Camellia sinensis) in response to low temperature stress [J].Journal of Tea Science,2018,38(4):396.
[16]冯玉磊.寒地冬小麦返青期抗氧化特性[J].麦类作物学报,2012,32(3):550.FENG Y L.Antioxidant properties of winter wheat in frigid region at greening stage [J].Journal of Triticeae Crops,2012,32(3):550.
[17]张晓红,冯梁杰,杨特武,等.冬季低温胁迫对油菜抗寒生理特性的影响[J].植物生理学报.2015,51(5):745.ZHANG X H,FENG L J,YANG T W,et al.Effects of chilling stress on physiological characteristics of rapeseed seedlings in winter [J].Plant Physiology Journal,2015,51(5):745.
[18]XIE D W,WANG X N,FU L S,et al.Identification of the trehalose-6-phosphate synthase gene family in winter wheat and expression analysis under conditions of freezing stress [J].Journal of Genetics,2015,94(1):56.
[19]FREDERIKS T M,CHRISTOPHER J T,HARVEY G L,et al.Current and emerging screening methods to identify post-head-emergence frost adaptation in wheat and barley [J].Journal of Experimental Botany,2012,63(15):5405.
[20]SHAHRYAR N,MAALI-AMIRIR.Metabolic acclimation of tetraploid and hexaploid wheats by cold stress-induced carbohydrate accumulation [J].Journal of Plant Physiology,2016,204:52.
[21]KALAPOS B,NOVáK A,DOBREV P,et al.Effect of the winter wheat cheyenne 5A substituted chromosome on dynamics of abscisic acid and cytokinins in freezing-sensitive Chinese Spring genetic background [J].Frontiers in Plant Science,2017,8:2033.
[22]TCHAGANG A B,FAUTEUX F,DAN T,et al.Bioinformatics identification of new targets for improving low temperature stress tolerance in spring and winter wheat [J].Bmc Bioinformatics,2017,18(1):174.
[23]赵瑞玲,赵勇,徐渴,等.室内冷冻法鉴定小麦抗寒性的研究[J/OL].植物遗传资源学报.https://doi.org/10.13430/j.cnki.jpgr.20180719001.ZHAO R L,ZHAO Y,XU K,et al.Identification of cold resistance wheat varieties by laboratory freezing method [J/OL] .Journal of Plant Genetic Resources.https://doi.org/10.13430/j.cnki.jpgr.20180719001.
[24]张淑霞,钟阳和,魏淑秋.实验室冷冻法鉴定2个冬小麦品种抗寒性研究[J].中国生态农业学报,2003,11(3):39.ZHANG S X,ZHONG Y H,WEI S Q.Thecold resistance of two winter wheat cultivars appraised by the freezing method in a experiment room [J].Chinese Journal of Eco-Agriculture,2003,11(3)39.
[25]李合生.现代植物生理学[M].北京:高等教育出版社,2012.LI H S.Modern plant physiology [M].Beijing:Higher Education Press,2012.
[26]邹琦.植物生理学实验指导[M].北京:高等教育出版社,2000.ZOU Q.Experimental guide for plant physiology [M].Beijing:Higher Education Press Higher Education Press,2000.
[27]陈建勋,王晓峰.植物生理学实验指导[M].广州:华南理工大学出版社,2002:120.CHEN J X,WANG X F.Experimental guide for plant physiology [M].Guangzhou:South China University of Technology Press,2002:120.
[28]INZé D.Extraction and determination of ascorbate and dehydroascorbate from plant tissue [J].Analytical Biochemistry,1995,225(1):165.
[29]MA F,CHENG L.The sun-exposed peel of apple fruit has higher xanthophyll cycle-dependent thermal dissipation and antioxidants of the ascorbate–glutathione pathway than the shaded peel [J].Plant Science,2003,165(4):821.
[30]ESIM N,ATICI O,MUTLU S.Effects of exogenous nitric oxide in wheat seedlings under chilling stress [J].Toxicology & Industrial Health,2012,30(3):270.
[31]ZHANG B,JIA D,GAO Z,et al.Physiological responses to low temperature in spring and winter wheat varieties [J].Journal of the Science of Food & Agriculture,2016,96(6):1969.
[32]ZHANG D,REN L,CHEN G Q,et al.ROS-induced oxidative stress and apoptosis-like event directly affect the cell viability of cry preserved embryogenic callus in Agapanthus praecox [J].Plant Cell Reports,2015,34(9):1505.
[33]SMIRNOFF N.The role of active oxygen in the response of plants to water deficit and desiccation [J].New Phytologist,1993,125(1):31.
[34]GILL S S,TUTEJAN.Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants [J].Plant Physiology & Biochemistry,2010,48(12):918.
[35]YOSHIMURA K.Expression of spinach ascorbate peroxidase isoenzymes in response to oxidative stresses [J].Plant Physiology,2000,123(1):225.
[36]MILLER A F.Superoxide dismutases:Ancient enzymes and new insights [J].Febs Letters,2012,586(5):587.
[37]DGRAHAM A,PATTERSON B D.Respo^nses of plants to low,nonfreezing temperatures:proteins,metabolism,and acclimation [J].Annu.Rev.Plant Physiol,2003,33(1):349.
[38]XU J.Comparative physiological and proteomic response to abrupt low temperature stress between two winter wheat cultivars differing in low temperature tolerance [J].Plant Biology,2013,15(2):297.
[39]WANG Y L,KANG J.Effects of low temperature stress on physiological and biochemical characteristics of winter wheat in seedling and jointing stages [J].Journal of Henan Agricultural Sciences,2004.