不同消毒剂对PRV、PPV和TGEV的灭活效果试验
|
摘要
为了确定癸甲溴铵-碘溶液消毒剂和癸甲溴铵消毒剂对猪主要疫病病原如猪细小病毒(PPV)、伪狂犬病病毒(PRV)和猪传染性胃肠炎病毒(TGEV)的灭活效果,通过细胞观察法,用不同浓度的癸甲溴铵-碘溶液消毒剂和癸甲溴铵消毒剂对PRV、PPV和TGEV进行灭活来观察其灭活效果。结果表明,癸甲溴铵-碘溶液和癸甲溴铵溶液对细胞的最大无毒剂量分别为1∶80和1∶10。1∶320的癸甲溴铵-碘溶液消毒剂,1∶80的癸甲溴铵消毒剂与相应浓度的中和剂的反应产物已无毒副作用。癸甲溴铵-碘溶液消毒剂1∶800稀释时可使PRV TCID_(50)降低5个滴度,TGEV TCID_(50)降低4个滴度;癸甲溴铵消毒剂稀释到1∶400时,可使PRV和TGEV的TCID_(50)均降低约3个滴度。两种消毒剂对PPV的灭活效果均不理想。
In order to determine the inactivation effects of deciquam and iodine compound disinfectant and deciquam disinfectant on porcine parvovirus(PPV),pseudorabies virus(PRV)and porcine infectious gastroenteritis virus(TGEV),PPV,PRV,TGEV were inactivated with different concentrations of deciquam and iodine compound disinfectant and deciquam disinfectant.The cell culture observation showed that the maximum non-toxic dose of deciquam and iodine compound disinfectant and deciquam disinfectant were 1∶80 and 1∶10 respectively.The corresponding concentrations of reaction products of 1∶32 deciquam and iodine compound disinfectant and 1∶80 deciquam disinfectant with neutralizer had no toxic side effects.When deciquam and iodine compound disinfectant was at 1∶800 dilution,the TCID50 to PRV was reduced by 5 titers,and to TGEV was reduced by 4 titers.When the dilution of deciquam was 1∶400,the TCID50 to PRV and TGEV can be reduced by 3 titers.While the effect of these two disinfectants on the inactivation of PPV was not good.
In order to determine the inactivation effects of deciquam and iodine compound disinfectant and deciquam disinfectant on porcine parvovirus(PPV),pseudorabies virus(PRV)and porcine infectious gastroenteritis virus(TGEV),PPV,PRV,TGEV were inactivated with different concentrations of deciquam and iodine compound disinfectant and deciquam disinfectant.The cell culture observation showed that the maximum non-toxic dose of deciquam and iodine compound disinfectant and deciquam disinfectant were 1∶80 and 1∶10 respectively.The corresponding concentrations of reaction products of 1∶32 deciquam and iodine compound disinfectant and 1∶80 deciquam disinfectant with neutralizer had no toxic side effects.When deciquam and iodine compound disinfectant was at 1∶800 dilution,the TCID50 to PRV was reduced by 5 titers,and to TGEV was reduced by 4 titers.When the dilution of deciquam was 1∶400,the TCID50 to PRV and TGEV can be reduced by 3 titers.While the effect of these two disinfectants on the inactivation of PPV was not good.
引文
[1] 陈谊,郑明.人猪共患疾病与感染 [M].北京:中国农业出版社,2017.
[2] 殷震,刘景华.动物病毒学(2版)[M].北京:科学出版社,1997.
[3] 杨汉春.猪伪狂犬病的流行现状与特点[J].猪业科学,2016,33(1):38-38.
[4] 刘立兵,王建昌,经美,等.猪细小病毒重组酶聚合酶扩增快速检测方法的建立[J].中国畜牧兽医,2017,44(11):3320-3326.
[5] 王赛,苏菲,袁秀芳,等.猪传染性胃肠炎病毒N 蛋白单克隆抗体的制备及鉴定[J].中国兽医科学,2018,48(1):13-18.
[6] 侯玉凤,周宇,王爱玲,等.戊二醛-癸甲溴铵复合剂对大肠埃希菌和金黄色葡萄球菌的杀菌作用研究[J].中国兽药杂志,2017,51(9):12-17.
[7] 赵紫印,王爱玲,郭抗抗,等.戊二醛-癸甲溴铵复合消毒剂对伪狂犬病病毒的灭活作用研究[J].中国消毒学杂志,2017,34(8):715-718.
[8] 董炳梅,杨丽梅,张含侠,等.猪种布鲁氏菌对4种常用新型消毒剂敏感性的研究[J].中国动物保健,2017,19(1):75-78.
[9] 陈小强,王晶钰,张中航,等.规模化鸡场消毒剂的筛选与消毒效果评价[J].动物医学进展,2011,32(11):85-89.
[10] 赖宝色,林秋敏.规模化猪场消毒剂的选择[J].福建畜牧兽医,2018,40(1):29-31.
[11] 佟海山,陈遇英.我国兽用消毒剂的应用现状与对策[J].中国兽药杂志,2017,53(9):115-117.
[12] 毛娅卿,郭致君,刘彤,等.两种消毒剂对伪狂犬病病毒的杀灭效果的实验观察[J].中国兽医杂志,2008,44(2):67-69.
[13] 古一雯,李临生,罗琥捷.消毒剂研究新进展[J].中国消毒学杂志,2009,25(1):70-73.
[14] 李建霞.几种常用消毒剂的消毒效果比较[J].畜牧兽医科学,2017,7(3):18-19.
[15] 乔利敏,于凤芝.消毒剂使用存在的问题及应对措施[J].山东畜牧兽医,2017,38(6):46-48.
[2] 殷震,刘景华.动物病毒学(2版)[M].北京:科学出版社,1997.
[3] 杨汉春.猪伪狂犬病的流行现状与特点[J].猪业科学,2016,33(1):38-38.
[4] 刘立兵,王建昌,经美,等.猪细小病毒重组酶聚合酶扩增快速检测方法的建立[J].中国畜牧兽医,2017,44(11):3320-3326.
[5] 王赛,苏菲,袁秀芳,等.猪传染性胃肠炎病毒N 蛋白单克隆抗体的制备及鉴定[J].中国兽医科学,2018,48(1):13-18.
[6] 侯玉凤,周宇,王爱玲,等.戊二醛-癸甲溴铵复合剂对大肠埃希菌和金黄色葡萄球菌的杀菌作用研究[J].中国兽药杂志,2017,51(9):12-17.
[7] 赵紫印,王爱玲,郭抗抗,等.戊二醛-癸甲溴铵复合消毒剂对伪狂犬病病毒的灭活作用研究[J].中国消毒学杂志,2017,34(8):715-718.
[8] 董炳梅,杨丽梅,张含侠,等.猪种布鲁氏菌对4种常用新型消毒剂敏感性的研究[J].中国动物保健,2017,19(1):75-78.
[9] 陈小强,王晶钰,张中航,等.规模化鸡场消毒剂的筛选与消毒效果评价[J].动物医学进展,2011,32(11):85-89.
[10] 赖宝色,林秋敏.规模化猪场消毒剂的选择[J].福建畜牧兽医,2018,40(1):29-31.
[11] 佟海山,陈遇英.我国兽用消毒剂的应用现状与对策[J].中国兽药杂志,2017,53(9):115-117.
[12] 毛娅卿,郭致君,刘彤,等.两种消毒剂对伪狂犬病病毒的杀灭效果的实验观察[J].中国兽医杂志,2008,44(2):67-69.
[13] 古一雯,李临生,罗琥捷.消毒剂研究新进展[J].中国消毒学杂志,2009,25(1):70-73.
[14] 李建霞.几种常用消毒剂的消毒效果比较[J].畜牧兽医科学,2017,7(3):18-19.
[15] 乔利敏,于凤芝.消毒剂使用存在的问题及应对措施[J].山东畜牧兽医,2017,38(6):46-48.