米氏凯伦藻(Km02)共培养细菌群落的研究
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摘要
米氏凯伦藻作为我国沿岸典型的有毒赤潮甲藻,近年来赤潮频度越来越密,赤潮规模越来越大,持续时间也越来越长,造成了严重的经济损失,并且威胁到海洋生态系统的健康。众多研究表明藻际细菌可能是影响赤潮生消与有毒藻产毒的关键生物影响因子。为揭示米氏凯伦藻共培养菌群的物种多样性信息,本文通过高通量测序技术手段,分析了我国沿岸产米氏凯伦藻(Km02)共培养菌群的种类、丰度、多样性及系统发育信息。结果显示,米氏凯伦藻(Km02)共培养菌群共有40个种与已有数据库匹配,隶属于4个门(Phylum),11个纲(Class),21个目(Order),26个科(Family),37个属(Genus)。其中优势菌属有8个(>5%),分别为Balneola(20.28%)、 Marinobacter(19.25%)、Rhodobacteraceae科下的未知属(13.15%)、Alteromonas (8.88%)、Methylophaga (8.17%)、Thalassospira(6.24%)、Reichenbachiella (5.92%)及Mesoflavibacter (5.41%)。研究结果表明我国沿岸产米氏凯伦藻(Km02)含有生物多样性较高的共培养菌群,且具有探寻未知海洋细菌的潜力,同时该研究结果也为未来更进一步研究细菌群落对米氏凯伦藻赤潮暴发与消亡,以及产毒特性等机制的研究提供了实验方法与奠定了理论基础。
Karenia mikimotoi is a typical toxic harmful algal along the coast of China, and its bloom becomes more frequent, bigger in scale, and lasts longer, and has caused heavy economic losses and threats to the health of the residents in recent years. Many studies have shown that bacteria in the phycosphere may play a key biological role that affects a harmful algal bloom and toxin production. To reveal the species diversity and composition of co-cultured bacterial community of K. mikimotoi, we analyzed bacterial species, relative abundance, diversity and phylogenetic information of co-culture bacterial communities of K. mikimotoi(Km02) via high-throughput sequencing. The OTU(operational taxonomic units) taxonomy analysis showed that 40 species co-cultured bacterial community associated with K. mikimotoi(Km02) could match the established database, including 4 phyla, 11 classes, 21 orders, 26 families, and 37 genera. The top eight species(>5%) included Balneola(20.28%), Marinobacter(19.25%), unknown species in Rhodobacteraceae(13.15%),Alteromonas(8.88%), Methylophaga(8.17%), Thalassospira(6.24%), Reichenbachiella(5.92%), and Mesoflavibacter(5.41%). This study is the first one reporting the biodiversity of co-cultured bacterial community associated with K.mikimotoi(Km02), providing a potential of discovering new marine microbial species from this strain, and a theoretical basis for understanding the mechanism of the bacterial community to the initiation and termination of the harmful algal bloom of K. mikimotoi, as well as the toxin production.
Karenia mikimotoi is a typical toxic harmful algal along the coast of China, and its bloom becomes more frequent, bigger in scale, and lasts longer, and has caused heavy economic losses and threats to the health of the residents in recent years. Many studies have shown that bacteria in the phycosphere may play a key biological role that affects a harmful algal bloom and toxin production. To reveal the species diversity and composition of co-cultured bacterial community of K. mikimotoi, we analyzed bacterial species, relative abundance, diversity and phylogenetic information of co-culture bacterial communities of K. mikimotoi(Km02) via high-throughput sequencing. The OTU(operational taxonomic units) taxonomy analysis showed that 40 species co-cultured bacterial community associated with K. mikimotoi(Km02) could match the established database, including 4 phyla, 11 classes, 21 orders, 26 families, and 37 genera. The top eight species(>5%) included Balneola(20.28%), Marinobacter(19.25%), unknown species in Rhodobacteraceae(13.15%),Alteromonas(8.88%), Methylophaga(8.17%), Thalassospira(6.24%), Reichenbachiella(5.92%), and Mesoflavibacter(5.41%). This study is the first one reporting the biodiversity of co-cultured bacterial community associated with K.mikimotoi(Km02), providing a potential of discovering new marine microbial species from this strain, and a theoretical basis for understanding the mechanism of the bacterial community to the initiation and termination of the harmful algal bloom of K. mikimotoi, as well as the toxin production.
引文
王朝晖,尹伊伟,齐雨藻等,2001.珠海桂山岛米氏裸甲藻赤潮对鱼鳃损伤的病理学组织观察.海洋学报,23(1):133-138
刘志国,王金辉,蔡芃等,2014.米氏凯伦藻分布及其引发赤潮的发生规律研究.国土与自然资源研究,(1):38-41
刘桂英,葛坤,宋伦等,2015.米氏凯伦藻的研究进展.海洋科学,39(9):117-122
许翠娅,黄美珍,杜琦,2010.福建沿岸海域主要赤潮生物的生态学特征.台湾海峡,29(3):434-441
张水浸,许昆灿,陈其焕等,1988.厦门西港区一次赤潮的观测.海洋学报,10(5):602-608
香港渔农自然护理署,2013.香港有害海洋微藻
曾江宁,曾淦宁,黄韦艮等,2004.赤潮影响因素研究进展.东海海洋,22(2):40-47
Anderson D M,Burkholder J M,Cochlan W P et al,2008.Harmful algal blooms and eutrophication:examining linkages from selected coastal regions of the United States.Harmful Algae,8(1):39-53
Azam F,Fenchel T,Field J G et al,1983.The ecological role of water-column microbes in the sea.Marine Ecology Progress Series,10(3):257-263
Blaxter M,Mann J,Chapman T et al,2005.Defining operational taxonomic units using DNA barcode data.Philosophical Transactions of the Royal Society B:Biological Sciences,360(1462):1935-1943
Bolch C J S,Subramanian T A,Green D H,2011.The toxic dinoflagellate Gymnodinium catenatum(dinophyceae)requires marine bacteria for growth.Journal of Phycology,47(5):1009-1022
Celikkol-Aydin S,Gaylarde C C,Lee T et al,2016.16S rRNAgene profiling of planktonic and biofilm microbial populations in the Gulf of Guinea using Illumina NGS.Marine Environmental Research,122:105-112
Croft M T,Lawrence A D,Raux-Deery E et al,2005.Algae acquire vitamin B12 through a symbiotic relationship with bacteria.Nature,438(7064):90-93
Dai X F,Lu D D,Guan W B et al,2014.Newly recorded Karlodinium veneficum dinoflagellate blooms in stratified water of the East China Sea.Deep Sea Research Part II:Topical Studies in Oceanography,101:237-243
Green D H,Llewellyn L E,Negri A P et al,2004.Phylogenetic and functional diversity of the cultivable bacterial community associated with the paralytic shellfish poisoning dinoflagellate Gymnodinium catenatum.FEMSMicrobiology Ecology,47(3):345-357
Grossart H P,Levold F,Allgaier M et al,2005.Marine diatom species harbour distinct bacterial communities.Environmental Microbiology,7(6):860-873
Guidi F,Pezzolesi L,Vanucci S,2018.Microbial dynamics during harmful dinoflagellate Ostreopsis cf.ovata growth:bacterial succession and viral abundance pattern.MicrobiologyOpen,7(4):e00584
Guillard R R L,Ryther J H,1962.Studies of marine planktonic diatoms:I.Cyclotella nana Hustedt,and Detonula confervacea(Cleve)Gran.Canadian Journal of Microbiology,8(2):229-239
Heisler J,Glibert P M,Burkholder J M et al,2008.Eutrophication and harmful algal blooms:a scientific consensus.Harmful Algae,8(1):3-13
Hold G L,Smith E A,RappéM S et al,2001.Characterisation of bacterial communities associated with toxic and non-toxic dinoflagellates:Alexandrium spp.and Scrippsiella trochoidea.FEMS Microbiology Ecology,37(2):161-173
Keeling P J,Palmer J D,2008.Horizontal gene transfer in eukaryotic evolution.Nature Reviews Genetics,9(8):605-618
Kim J H,Wang P B,Park B S et al,2018.Revealing the distinct habitat ranges and hybrid zone of genetic sub-populations within Pseudo-nitzschia pungens(Bacillariophyceae)in the West Pacific area.Harmful Algae,73:72-83
Lu D D,Goebel J,2001.Five red tide species in genus Prorocentrum including the description of Prorocentrum donghaiense Lu sp.nov.from the East China Sea.Chinese Journal of Oceanology and Limnology,19(4):337-344
Park B S,Kim J H,Kim J H et al,2015.Dynamics of bacterial community structure during blooms of Cochlodinium polykrikoides(Gymnodiniales,Dinophyceae)in Korean coastal waters.Harmful Algae,48:44-54
Park B S,Wang P B,Kim J H et al,2014.Resolving the intra-specific succession within Cochlodinium polykrikoides populations in southern Korean coastal waters via use of quantitative PCR assays.Harmful Algae,37:133-141
Park J,Park B S,Wang P B et al,2017.Phycospheric native bacteria Pelagibaca bermudensis and Stappia sp.ameliorate biomass productivity of Tetraselmis striata(KCTC1432BP)in co-cultivation system through mutualistic interaction.Frontiers in Plant Science,8:289
Ramanan R,Kim B H,Cho D H et al,2016.Algae-bacteria interactions:evolution,ecology and emerging applications.Biotechnology Advances,34(1):14-29
Rooney-Varga J N,Giewat M W,Savin M C et al,2005.Links between phytoplankton and bacterial community dynamics in a coastal marine environment.Microbial Ecology,49(1):163-175
Sarmento H,Gasol J M,2012.Use of phytoplankton‐derived dissolved organic carbon by different types of bacterioplankton.Environmental Microbiology,14(9):2348-2360
Wang P B,Joo J H,Park B S et al,2017.Relationship between dissolved organic carbon and bacterial community in the coastal waters of Incheon,Korea.Oceanological and Hydrobiological Studies,46(1):50-61
刘志国,王金辉,蔡芃等,2014.米氏凯伦藻分布及其引发赤潮的发生规律研究.国土与自然资源研究,(1):38-41
刘桂英,葛坤,宋伦等,2015.米氏凯伦藻的研究进展.海洋科学,39(9):117-122
许翠娅,黄美珍,杜琦,2010.福建沿岸海域主要赤潮生物的生态学特征.台湾海峡,29(3):434-441
张水浸,许昆灿,陈其焕等,1988.厦门西港区一次赤潮的观测.海洋学报,10(5):602-608
香港渔农自然护理署,2013.香港有害海洋微藻
曾江宁,曾淦宁,黄韦艮等,2004.赤潮影响因素研究进展.东海海洋,22(2):40-47
Anderson D M,Burkholder J M,Cochlan W P et al,2008.Harmful algal blooms and eutrophication:examining linkages from selected coastal regions of the United States.Harmful Algae,8(1):39-53
Azam F,Fenchel T,Field J G et al,1983.The ecological role of water-column microbes in the sea.Marine Ecology Progress Series,10(3):257-263
Blaxter M,Mann J,Chapman T et al,2005.Defining operational taxonomic units using DNA barcode data.Philosophical Transactions of the Royal Society B:Biological Sciences,360(1462):1935-1943
Bolch C J S,Subramanian T A,Green D H,2011.The toxic dinoflagellate Gymnodinium catenatum(dinophyceae)requires marine bacteria for growth.Journal of Phycology,47(5):1009-1022
Celikkol-Aydin S,Gaylarde C C,Lee T et al,2016.16S rRNAgene profiling of planktonic and biofilm microbial populations in the Gulf of Guinea using Illumina NGS.Marine Environmental Research,122:105-112
Croft M T,Lawrence A D,Raux-Deery E et al,2005.Algae acquire vitamin B12 through a symbiotic relationship with bacteria.Nature,438(7064):90-93
Dai X F,Lu D D,Guan W B et al,2014.Newly recorded Karlodinium veneficum dinoflagellate blooms in stratified water of the East China Sea.Deep Sea Research Part II:Topical Studies in Oceanography,101:237-243
Green D H,Llewellyn L E,Negri A P et al,2004.Phylogenetic and functional diversity of the cultivable bacterial community associated with the paralytic shellfish poisoning dinoflagellate Gymnodinium catenatum.FEMSMicrobiology Ecology,47(3):345-357
Grossart H P,Levold F,Allgaier M et al,2005.Marine diatom species harbour distinct bacterial communities.Environmental Microbiology,7(6):860-873
Guidi F,Pezzolesi L,Vanucci S,2018.Microbial dynamics during harmful dinoflagellate Ostreopsis cf.ovata growth:bacterial succession and viral abundance pattern.MicrobiologyOpen,7(4):e00584
Guillard R R L,Ryther J H,1962.Studies of marine planktonic diatoms:I.Cyclotella nana Hustedt,and Detonula confervacea(Cleve)Gran.Canadian Journal of Microbiology,8(2):229-239
Heisler J,Glibert P M,Burkholder J M et al,2008.Eutrophication and harmful algal blooms:a scientific consensus.Harmful Algae,8(1):3-13
Hold G L,Smith E A,RappéM S et al,2001.Characterisation of bacterial communities associated with toxic and non-toxic dinoflagellates:Alexandrium spp.and Scrippsiella trochoidea.FEMS Microbiology Ecology,37(2):161-173
Keeling P J,Palmer J D,2008.Horizontal gene transfer in eukaryotic evolution.Nature Reviews Genetics,9(8):605-618
Kim J H,Wang P B,Park B S et al,2018.Revealing the distinct habitat ranges and hybrid zone of genetic sub-populations within Pseudo-nitzschia pungens(Bacillariophyceae)in the West Pacific area.Harmful Algae,73:72-83
Lu D D,Goebel J,2001.Five red tide species in genus Prorocentrum including the description of Prorocentrum donghaiense Lu sp.nov.from the East China Sea.Chinese Journal of Oceanology and Limnology,19(4):337-344
Park B S,Kim J H,Kim J H et al,2015.Dynamics of bacterial community structure during blooms of Cochlodinium polykrikoides(Gymnodiniales,Dinophyceae)in Korean coastal waters.Harmful Algae,48:44-54
Park B S,Wang P B,Kim J H et al,2014.Resolving the intra-specific succession within Cochlodinium polykrikoides populations in southern Korean coastal waters via use of quantitative PCR assays.Harmful Algae,37:133-141
Park J,Park B S,Wang P B et al,2017.Phycospheric native bacteria Pelagibaca bermudensis and Stappia sp.ameliorate biomass productivity of Tetraselmis striata(KCTC1432BP)in co-cultivation system through mutualistic interaction.Frontiers in Plant Science,8:289
Ramanan R,Kim B H,Cho D H et al,2016.Algae-bacteria interactions:evolution,ecology and emerging applications.Biotechnology Advances,34(1):14-29
Rooney-Varga J N,Giewat M W,Savin M C et al,2005.Links between phytoplankton and bacterial community dynamics in a coastal marine environment.Microbial Ecology,49(1):163-175
Sarmento H,Gasol J M,2012.Use of phytoplankton‐derived dissolved organic carbon by different types of bacterioplankton.Environmental Microbiology,14(9):2348-2360
Wang P B,Joo J H,Park B S et al,2017.Relationship between dissolved organic carbon and bacterial community in the coastal waters of Incheon,Korea.Oceanological and Hydrobiological Studies,46(1):50-61
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