紫花苞舌兰类原球茎诱导及植株再生
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摘要
为建立紫花苞舌兰类原球茎离体再生体系,本研究以其腋芽为外植体,研究了不同激素浓度配比、添加剂对其类原球茎诱导、分化及生根诱导的影响。结果表明,外植体在1/2 MS+6-BA2.0 mg/L+NAA0.2 mg/L+AC(活性炭)0.2 g/L+CW(椰汁)10%培养基上培养35 d后,获得无菌苗,其成活率达80%以上;无菌苗的顶端分生组织在1/2 MS+10 mg/L Picloram培养基上的类原球茎的诱导率最高,达75%;类原球茎在1/2 MS+6-BA 2.0 mg/L+IBA 0.2 mg/L培养基上培养45 d后分化出小苗,分化率为54%;小苗在1/2 MS+6-BA 0.5 mg/L+IBA 1.0 mg/L+AC 0.2 g/L培养基上生根培养35 d后,生根率达85%以上;小苗驯化移栽成活率达95%,长势良好。
In order to establish a regeneration system in Spathoglottis plicata, its axillary bud was used as explant to study the effect of different hormone concentration and additives on PLBs induction, differentiation and rooting.The results showed that sterile plantlets were obtained under the condition of 1/2 MS +2.0 mg/L 6-BA+0.2 mg/L NAA+0.2 g/L AC(activated carbon)+10%, CW(coconut juice) after 35 days and its survival rate was higher that80%. The PLBs induction of apical meristem reached its top under the condition of 10 mg/L Picloram +1/2 MS,and the highest induction rate of PLBs clusters was 75%. Plantlets were differentiated from the PLBs under the condition of 1/2 MS+2.0 mg/L 6-BA+0.2 mg/L IBA after 45 d and the differentiation rate was 54%. The regeneration rate could reach up to 85% under the condition of 1.0 mg/L IBA+0.5 mg/L 6-BA+0.2 g/L AC after35 days. The survival rate of transplanted seedlings reached 95% with excellent growth.
In order to establish a regeneration system in Spathoglottis plicata, its axillary bud was used as explant to study the effect of different hormone concentration and additives on PLBs induction, differentiation and rooting.The results showed that sterile plantlets were obtained under the condition of 1/2 MS +2.0 mg/L 6-BA+0.2 mg/L NAA+0.2 g/L AC(activated carbon)+10%, CW(coconut juice) after 35 days and its survival rate was higher that80%. The PLBs induction of apical meristem reached its top under the condition of 10 mg/L Picloram +1/2 MS,and the highest induction rate of PLBs clusters was 75%. Plantlets were differentiated from the PLBs under the condition of 1/2 MS+2.0 mg/L 6-BA+0.2 mg/L IBA after 45 d and the differentiation rate was 54%. The regeneration rate could reach up to 85% under the condition of 1.0 mg/L IBA+0.5 mg/L 6-BA+0.2 g/L AC after35 days. The survival rate of transplanted seedlings reached 95% with excellent growth.
引文
Bapat V.A.,and Narayanaswami S.,1977,Rhizogenesis in a tis sue culture of the orchid Spathoglottis plicata,Bull.Torrey Bot.Club 104(1):2-4
Chen Y.Q.,1997,In vitro clonal propagation of Spathoglottis plicata,Zhiwu Shenglixue Tongxun(Plant Physiology Com munications),33(4):273-274(陈永勤,1997,苞舌兰的离体无性繁殖,植物生理学通讯,33(4):273-274)
Hossain M.M.,and Dey R.,2013,Multiple regeneration path ways in Spathoglottis plicata Blume-A study in vitro,South African Journal of Botany,85(2):56-62
Li F.F.,Yi C.,Li Q.F.,Zeng B.,and Wen S.,2014,Tissue culture of orchid Browning phenomenon and control,Nanfang Yuanyi(Southern Horticulture),25(4):50-53(李菲菲,易春,李青峰,曾斌,文帅,2014,兰花组织培养的褐化现象及控制研究进展,南方园艺,25(4):50-53)
Sebastinraj J.,and Muhirkuzhali S.,2014,Asymbiotic seed ger minati on and micropropagation of Spathoglottis plicat blume,IJAPBC,3(2):495-501
Sinha P.,Hakim M.L.,and Alam M.F.,2009,In vitro mass clona propagation of Spathoglottis plicata blume,Plant Tissue Cul ture&Biotechnology,19(2):151-160
Teng W.L.,Nicholson L.,and Teng M.C.,1997,Micropropaga tion of Spathoglottis plicata,Plant Cell Reports,16(12)831-835
Wang L.P.,and Liang S.Y.,2010,Study on PLB induction and propagation of Dendrobium officinale,Zhongguo Nongxu Tongbao(Chinese Agricultural Science Bulletin),26(1)265-268(王丽萍,梁淑云,2010,铁皮石斛原球茎诱导与增殖研究,中国农学通报,26(1):265-268)
Yao J.,Liu F.,Huang M.Z.,Tang Y.Q.,and Yang G.S.,2015,In vitro regeneration and browning of Renanthera coccine Loureiro stem buds,Redai Nongye Kexue(Chinese Journa of Tropical Agriculture),35(3):18-22(姚婧,刘帆,黄民忠唐燕琼,杨光穗,2015,火焰兰茎段侧芽离体再生及褐化的研究,热带农业科学,35(3):18-22)
Chen Y.Q.,1997,In vitro clonal propagation of Spathoglottis plicata,Zhiwu Shenglixue Tongxun(Plant Physiology Com munications),33(4):273-274(陈永勤,1997,苞舌兰的离体无性繁殖,植物生理学通讯,33(4):273-274)
Hossain M.M.,and Dey R.,2013,Multiple regeneration path ways in Spathoglottis plicata Blume-A study in vitro,South African Journal of Botany,85(2):56-62
Li F.F.,Yi C.,Li Q.F.,Zeng B.,and Wen S.,2014,Tissue culture of orchid Browning phenomenon and control,Nanfang Yuanyi(Southern Horticulture),25(4):50-53(李菲菲,易春,李青峰,曾斌,文帅,2014,兰花组织培养的褐化现象及控制研究进展,南方园艺,25(4):50-53)
Sebastinraj J.,and Muhirkuzhali S.,2014,Asymbiotic seed ger minati on and micropropagation of Spathoglottis plicat blume,IJAPBC,3(2):495-501
Sinha P.,Hakim M.L.,and Alam M.F.,2009,In vitro mass clona propagation of Spathoglottis plicata blume,Plant Tissue Cul ture&Biotechnology,19(2):151-160
Teng W.L.,Nicholson L.,and Teng M.C.,1997,Micropropaga tion of Spathoglottis plicata,Plant Cell Reports,16(12)831-835
Wang L.P.,and Liang S.Y.,2010,Study on PLB induction and propagation of Dendrobium officinale,Zhongguo Nongxu Tongbao(Chinese Agricultural Science Bulletin),26(1)265-268(王丽萍,梁淑云,2010,铁皮石斛原球茎诱导与增殖研究,中国农学通报,26(1):265-268)
Yao J.,Liu F.,Huang M.Z.,Tang Y.Q.,and Yang G.S.,2015,In vitro regeneration and browning of Renanthera coccine Loureiro stem buds,Redai Nongye Kexue(Chinese Journa of Tropical Agriculture),35(3):18-22(姚婧,刘帆,黄民忠唐燕琼,杨光穗,2015,火焰兰茎段侧芽离体再生及褐化的研究,热带农业科学,35(3):18-22)