SiO_2-PEG药物洗脱支架涂层的左旋多巴改性研究
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摘要
以正硅酸四乙酯和聚乙二醇为前驱体,利用左旋多巴(L-DOPA)的黏结性和自交联性能,制备左旋多巴改性的二氧化硅-聚乙二醇药物洗脱支架涂层,进而装载阿司匹林.采用金相显微镜观察涂层表面形貌,用紫外分光光度法考察载药涂层中阿司匹林的体外释放行为.结果表明,添加L-DOPA可延缓阿司匹林的释放,但L-DOPA加入过多可导致药物释放速度加快.涂层中阿司匹林的释放速率随着其装载浓度的增加而加快.制备涂层时的搅拌温度升高导致涂层溶剂挥发加快,进而改变涂层结构,导致阿司匹林的释放速度加快.
Owning to L-DOPA's adhesive and self-crosslinking properties, L-DOPA modified SiO_2-PEG coatings for drug eluting stents were prepared using TEOS and PEG as precursors, followed by the loading of aspirin as model drug. The surface morphology was characterized by metalloscope, and the in vitro release profiles of aspirin from coatings were investigated by UV/Vis analysis. The results indicated that L-DOPA resulted in the delayed release of aspirin, however, excessive L-DOPA resulted in the accelerated release. With the increase of aspirin loading concentration, the increase release rate was observed. The increase of stirring temperature during preparation resulted in the acceleration of solvent evaporation, leading to the accelerated release of aspirin.
Owning to L-DOPA's adhesive and self-crosslinking properties, L-DOPA modified SiO_2-PEG coatings for drug eluting stents were prepared using TEOS and PEG as precursors, followed by the loading of aspirin as model drug. The surface morphology was characterized by metalloscope, and the in vitro release profiles of aspirin from coatings were investigated by UV/Vis analysis. The results indicated that L-DOPA resulted in the delayed release of aspirin, however, excessive L-DOPA resulted in the accelerated release. With the increase of aspirin loading concentration, the increase release rate was observed. The increase of stirring temperature during preparation resulted in the acceleration of solvent evaporation, leading to the accelerated release of aspirin.
引文
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[3] SUN D M,ZHENG Y M,YIN T Y,et al.Coronary drug-eluting stents:from design optimization to newer strategies[J].Journal of Biomedical Materials Research:Part A,2014,102(5):1625-1640.
[4] 元峰,裴佳,袁广银.可生物降解/吸收药物洗脱支架临床应用现状综述[J].功能材料,2018,49(5):5028-5034.
[5] BAVRY A A,PARK K.Drug eluting stents[J].Journal of the American College of Cardiology,2016,67(12):1470-1471.
[6] SERRUYS P W,CHEVALIER,DUDEK D,et al.A bioresorbable everolimus- eluting scaffold versus a metallic everolimus- eluting stent for ischaemic heart disease caused by de-novo native coronary artery lesions (ABSORB II):an interim 1-year analysis of clinical and procedural secondary outcomes from a randomised controlled trial[J].The Lancet,2015,385(9962):43-54.
[7] Antithrombotic Trialists’Collaboration.Collaborative meta-analysis collaborative meta-analysis of randomized trials of antiplatelet therapy for prevention of death,myocardial infarction,and stroke in high risk patients[J].BMJ Journal,2002,324:71-86.
[8] 陈文平,詹红兵,李昭辉,等.左旋多巴改性有机-无机复合药物洗脱支架涂层的制备[J].中国组织工程研究与临床康复,2010(38):7112-7115.
[9] 蒋燕,向虹,游来江,等.SiO2基药物可控释放材料[J].材料导报,2006,20(1):50-53.
[10] 王莉玮,王子忱,赵敬哲,等.PEG法合成多孔高比表面积SiO2[J].功能材料,1998,29(4):397-400.
[11] HOLTEN-ANDERSON N,WAITE J H.Mussel-designed protective coatings for compliant substrates[J].Journal of Dental Research,2008,87(8):701-709.
[12] ZHU L P,YU J Z,XU Y Y,et al.Surface modification of PVDF porous membranes via poly(DOPA) coating and heparin immobilization[J].Colloids and Surfaces B:Biointerfaces,2009,69(1):152-155.
[13] 朱利平,徐又一,奚振宇,等.DOPA在聚乙烯微孔膜上的自聚合及肝素固定化[J].高分子学报,2009(4):394-397.
[14] LEE H,SCHERER N F,MESSERSMITH P B.Single molecule mechanics of mussel adhesion[J].Proceedings of the Natl Acad Sci USA,2006,103(35):12999-13003.
[15] DALSIN J L,Lin L J,TOSATTI S,et al.Protein resistance of titanium oxide surfaces modified by biologically inspired mPEG-DOPA [J].Langmuir,2005,21(2):640-646.
[16] CIHLA J.Hydrolysis and polycondensation of ethyl silicates.1.Effect of pH and catalyst on the hydrolysis and polycondensation of tetraethoxysilane (TEOS)[J].Colloids and Surfaces A:Physicochemical and Engineering Aspects,1993,70(3):239-251.
[17] 隋学叶,刘世权,程新.正硅酸乙酯的水解缩聚反应及多孔SiO2粉体的制备[J].中国粉体技术,2006,12(3):35-40.
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