乳杆菌对生物膜的作用及其机制研究进展
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摘要
慢性炎症和感染性疾病与致病菌生物膜(biofilm,BF)的形成密切相关,目前治疗BF相关疾病还局限于应用抗生素,但抗生素滥用会导致多重耐药,治疗不彻底会引起慢性感染,预防和治疗BF相关疾病的新方法亟待提出。随着益生菌生物制剂应用价值的不断体现与验证,益生菌生物制剂不断发展为消除某些致病菌BF感染的新方案。其中发展的最好的莫过于乳杆菌活菌制剂,大量的科研文献及临床实践已证明其具有强大的益生潜力,特别是在抑制致病菌生物膜方面,这为临床治疗BF相关感染开拓了新视野。本文主要综述当前国内外乳杆菌对生物膜作用及其机制的研究进展,旨在通过综述相关研究进展为临床治疗生物膜相关感染提供全新思路。
Chronic inflammation and infectious diseases are closely related to the formation of biofilm(BF). Currently, the treatment of BF-related diseases is limited to the application of antibiotics. But antibiotic abuse can lead to multiple drug resistance, and incomplete treatment can cause chronic infection. Therefore, new methods for treating BF-related diseases are urgently needed. With continuous demonstration and verification of the application value of probiotics, the biological agents have been developed as a new solution to eliminate some BF-related infections with pathogenic bacteria. Among them, live Lactobacillus preparation has become the most developed probiotic biological agent. A large number of scientific researches and clinical practices have proved that Lactobacillus has strong probiotic potential, especially in inhibition of pathogenic bacterial biofilm, which opens up a new horizon for the clinical treatment of biofilm-related infections. This paper reviews the current progress in research on the effect of Lactobacillus on bacterial biofilm and its mechanism, in order to provide a new idea for clinical treatment of biofilm-related infections.
Chronic inflammation and infectious diseases are closely related to the formation of biofilm(BF). Currently, the treatment of BF-related diseases is limited to the application of antibiotics. But antibiotic abuse can lead to multiple drug resistance, and incomplete treatment can cause chronic infection. Therefore, new methods for treating BF-related diseases are urgently needed. With continuous demonstration and verification of the application value of probiotics, the biological agents have been developed as a new solution to eliminate some BF-related infections with pathogenic bacteria. Among them, live Lactobacillus preparation has become the most developed probiotic biological agent. A large number of scientific researches and clinical practices have proved that Lactobacillus has strong probiotic potential, especially in inhibition of pathogenic bacterial biofilm, which opens up a new horizon for the clinical treatment of biofilm-related infections. This paper reviews the current progress in research on the effect of Lactobacillus on bacterial biofilm and its mechanism, in order to provide a new idea for clinical treatment of biofilm-related infections.
引文
[1] YANG Jing,CHEN Lihua.The relation between Pseudomonas aeruginosa biofilm and the host immunity[J].Chin J Microecol,2017,29(7):861-865.(in Chinese) 杨婧,陈丽华.铜绿假单胞菌生物被膜与宿主免疫的关系[J].中国微生态学杂志,2017,29(7):861-865.
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[5] WANG Qinhua.Influence of Lactobacillus acidophilus on Streptococcus mutans in vitro[D].Chongqing:ChongQing Medical University,2013.(in Chinese) 王勤花.乳酸杆菌对变形链球菌体外作用研究[D].重庆:重庆医科大学,2013.
[6] Wu CC,Lin CT,Wu CY,et al.Inhibitory effect of Lactobacillus salivarius on Streptococcus mutans biofilm formation[J].Mol Oral Microbiol,30(1):16-26.
[7] Chuang LC,Huang CS,Ou-Yang LW,et al.Probiotic Lactobacillus paracasei effect on cariogenic bacterial flora[J].Clin Oral Invest,2011,15(4):471-476.
[8] WANG Liangliang.The mechanism of lactic acid bacteria on preventing Staphylococcus aureus mastitis[D].Shaanxi:Northwest A&F University,2016.(in Chinese) 王亮亮.乳酸菌预防金黄色葡萄球菌乳腺炎的机制研究[D].陕西:西北农林科技大学,2016.
[9] Herman-Bausier P,El-Kirat-Chatel S,Foster TJ,et al.Staphylococcus aureus fibronectin-binding protein A mediates cell-cell adhesion through low-affinity homophilic bonds[J].Mbio,2015,6(3):e00413-15.
[10] Varma P,Nisha N,Dinesh KR,et al.Anti-infective properties of Lactobacillus fermentum against Staphylococcus aureus and Pseudomonas aeruginosa[J].J Mol Microbiol Biotechnol,2011,20(3):137-143.
[11] Prince T,McBain AJ,O′Neill CA.Lactobacillus reuteri protects epidermal keratinocytes from Staphylococcus aureus-induced cell death by competitive exclusion[J].Appl Environ Microbiol,2012,78(15):5119-5126.
[12] Pamer EG.Resurrecting the intestinal microbiota to combat antibiotic-resistant pathogens[J].Science,2016,352(6285):535-538.
[13] Nordmann P,Cuzon G,Naas T.The real threat of Klebsiella pneumoniae carbapenemase-producing bacteria[J].Lancet Infect Dis,2009,9(4):228-236.
[14] Rao KP,Chennappa G,Suraj U,et al.Probiotic potential of Lactobacillus strains isolated from sorghum-based traditional fermented food[J].Probiotics Antimicrob Proteins,2015,7(2):146-156.
[15] Hennequin C,Aumeran C,Robin F,et al.Antibiotic resistance and plasmid transfer capacity in biofilm formed with a CTX-M-15-producing Klebsiella pneumoniae isolate[J].J Antimicrob Chemother,2012,67(9):2123-2130.
[16] AI-Mathkhury HJ,Ali AS,Ghafil JA.Antagonistic effect of bacteriocin against urinary catheter associated Pseudomonads aeruginosa biofilm[J].N Am J Med Sci,2011,3(8):367-370.
[17] Ramos AN,Gobbato N,Rachid M,et al.Effect of Lactobacillus plantarum and Pseudomonas aeruginosa culture supernatants on polymorphonuclear damage and inflammatory response[J].Int Immunopharmacol,2010,10(2):247-251.
[18] LI Ruilian,WANG Zhuo,DU Yuguang.Recent progress in Candida albicans biofilm[J].Acta Microgiol Sin,2017,57(8):1206-1218.(in Chinese) 李瑞莲,王倬,杜昱光.白假丝酵母菌生物膜研究进展[J].微生物学报,2017,57(8):1206-1218.
[19] Chew SY,Cheah YK,Seow HF,et al.In vitro modulation of probiotic bacteria on the biofilm of Candida glabrata[J].Anaerobe,2015,34:132-138.
[20] Rizzello CG,Filannino P,Di Cagno R,et al.Quorum-sensing regulation of constitutive plantaricin by Lactobacillus plantarum strains under a model system for vegetables and fruits[J].Appl Environ Microbiol,2014,80(2):777-787.
[21] Maldonado-Barragan A,Caballero-Guerrero B,Lucena-Padros H,et al.Induction of bacteriocin production by coculture is widespread among plantaricin-producing Lactobacillus plantarum strains with different regulatory operons[J].Food Microbiol,2013,33(1):40-47.
[22] James KM,MacDonald KW,Chanyi RM,et al.Inhibition of Candida albicans biofilm formation and modulation of gene expression by probiotic cells and supernatant[J].J Med Microbiol,2016,65(4):328-336.
[23] Martinez RC,Franceschini SA,Patta MC,et al.Reid,Improved treatment of vulvovaginal candidiasis with fluconazole plus probiotic Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14[J].Lett Appl Microbiol,2009,48(3):269-274.
[24] Chew SY,Cheah YK,Seow HF,et al.In vitro modulation of probiotic bacteria on the biofilm of Candida glabrata[J].Anaerobe,2015,34:132-138.
[2] Ma L,Wang J,Wang S,et al.Synthesis of multiple Pseudomonas aeruginosa biofilm matrix exopolysaccharides is post-transcriptionally regulated[J].Environ Microbiol,2012,14(8):1995-2005.
[3] Desai JV,Mitchell AP.Candida albicans biofilm development and its genetic control[J].Microbiol Spectr,2015,3(3):10-16.
[4] Dosler S,Karaaslan E.Inhibition and destruction of Pseudomonas aeruginosa,biofilms by antibiotics and antimicrobial peptides[J].Peptides,2014,62:32-37.
[5] WANG Qinhua.Influence of Lactobacillus acidophilus on Streptococcus mutans in vitro[D].Chongqing:ChongQing Medical University,2013.(in Chinese) 王勤花.乳酸杆菌对变形链球菌体外作用研究[D].重庆:重庆医科大学,2013.
[6] Wu CC,Lin CT,Wu CY,et al.Inhibitory effect of Lactobacillus salivarius on Streptococcus mutans biofilm formation[J].Mol Oral Microbiol,30(1):16-26.
[7] Chuang LC,Huang CS,Ou-Yang LW,et al.Probiotic Lactobacillus paracasei effect on cariogenic bacterial flora[J].Clin Oral Invest,2011,15(4):471-476.
[8] WANG Liangliang.The mechanism of lactic acid bacteria on preventing Staphylococcus aureus mastitis[D].Shaanxi:Northwest A&F University,2016.(in Chinese) 王亮亮.乳酸菌预防金黄色葡萄球菌乳腺炎的机制研究[D].陕西:西北农林科技大学,2016.
[9] Herman-Bausier P,El-Kirat-Chatel S,Foster TJ,et al.Staphylococcus aureus fibronectin-binding protein A mediates cell-cell adhesion through low-affinity homophilic bonds[J].Mbio,2015,6(3):e00413-15.
[10] Varma P,Nisha N,Dinesh KR,et al.Anti-infective properties of Lactobacillus fermentum against Staphylococcus aureus and Pseudomonas aeruginosa[J].J Mol Microbiol Biotechnol,2011,20(3):137-143.
[11] Prince T,McBain AJ,O′Neill CA.Lactobacillus reuteri protects epidermal keratinocytes from Staphylococcus aureus-induced cell death by competitive exclusion[J].Appl Environ Microbiol,2012,78(15):5119-5126.
[12] Pamer EG.Resurrecting the intestinal microbiota to combat antibiotic-resistant pathogens[J].Science,2016,352(6285):535-538.
[13] Nordmann P,Cuzon G,Naas T.The real threat of Klebsiella pneumoniae carbapenemase-producing bacteria[J].Lancet Infect Dis,2009,9(4):228-236.
[14] Rao KP,Chennappa G,Suraj U,et al.Probiotic potential of Lactobacillus strains isolated from sorghum-based traditional fermented food[J].Probiotics Antimicrob Proteins,2015,7(2):146-156.
[15] Hennequin C,Aumeran C,Robin F,et al.Antibiotic resistance and plasmid transfer capacity in biofilm formed with a CTX-M-15-producing Klebsiella pneumoniae isolate[J].J Antimicrob Chemother,2012,67(9):2123-2130.
[16] AI-Mathkhury HJ,Ali AS,Ghafil JA.Antagonistic effect of bacteriocin against urinary catheter associated Pseudomonads aeruginosa biofilm[J].N Am J Med Sci,2011,3(8):367-370.
[17] Ramos AN,Gobbato N,Rachid M,et al.Effect of Lactobacillus plantarum and Pseudomonas aeruginosa culture supernatants on polymorphonuclear damage and inflammatory response[J].Int Immunopharmacol,2010,10(2):247-251.
[18] LI Ruilian,WANG Zhuo,DU Yuguang.Recent progress in Candida albicans biofilm[J].Acta Microgiol Sin,2017,57(8):1206-1218.(in Chinese) 李瑞莲,王倬,杜昱光.白假丝酵母菌生物膜研究进展[J].微生物学报,2017,57(8):1206-1218.
[19] Chew SY,Cheah YK,Seow HF,et al.In vitro modulation of probiotic bacteria on the biofilm of Candida glabrata[J].Anaerobe,2015,34:132-138.
[20] Rizzello CG,Filannino P,Di Cagno R,et al.Quorum-sensing regulation of constitutive plantaricin by Lactobacillus plantarum strains under a model system for vegetables and fruits[J].Appl Environ Microbiol,2014,80(2):777-787.
[21] Maldonado-Barragan A,Caballero-Guerrero B,Lucena-Padros H,et al.Induction of bacteriocin production by coculture is widespread among plantaricin-producing Lactobacillus plantarum strains with different regulatory operons[J].Food Microbiol,2013,33(1):40-47.
[22] James KM,MacDonald KW,Chanyi RM,et al.Inhibition of Candida albicans biofilm formation and modulation of gene expression by probiotic cells and supernatant[J].J Med Microbiol,2016,65(4):328-336.
[23] Martinez RC,Franceschini SA,Patta MC,et al.Reid,Improved treatment of vulvovaginal candidiasis with fluconazole plus probiotic Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14[J].Lett Appl Microbiol,2009,48(3):269-274.
[24] Chew SY,Cheah YK,Seow HF,et al.In vitro modulation of probiotic bacteria on the biofilm of Candida glabrata[J].Anaerobe,2015,34:132-138.