肝细胞自体荧光光谱研究
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摘要
攻克肝癌需要对肝细胞有足够的了解,才能进而对比肝细胞与肝癌细胞的区别,实现肝癌的早期筛查工作。首先,在实验室中培育了HL-7702[L-02]肝细胞,接着利用荧光光谱仪研究了肝细胞的自体荧光光谱。结果表明,肝细胞的荧光光谱出现两个明显峰值,分别位于588nm和660nm处,其中588nm处获得最大荧光强度。然后,结合荧光显微镜观察肝细胞的形态,计算多个视野下的细胞荧光图像,获得了肝细胞平均直径接近13.1μm。流式细胞仪的实验表明肝细胞的直径比较集中。最后,研究了肝细胞荧光饱和强度的变化,发现随着细胞浓度增加,肝细胞会呈现荧光饱和状态。实验结果为深入研究肝细胞提供了理论依据,为接下来肝细胞及肝癌细胞对比研究打下基础。
In order to overcome the need for liver cells to understand liver cancer,the difference can be compared between liver cells and liver cancer cells, and the screening of liver cancer can be achieved early. Firstly, HL-7702[L-02] hepatic cell was cultured in the laboratory,and then the autofluorescence spectrum of hepatic cell were studied by fluorescence spectrometry. The results showed that the fluorescence spectrum of hepatic cell showed two distinct peaks at 588nm and 660nm,respectively,and the maximum fluorescence intensity was obtained at 588nm. Then,the morphology of the hepatic cell was observed by fluorescence microscopy,and the fluorescence photographs of the cells in multiple fields of view were calculated,and the average diameter of the hepatic cell was obtained to be close to 13.1μm. The experiment of flow cytometer shows that the diameter of hepatic cell was relatively concentrated. Finally,the changes of fluorescence saturation intensity of hepatic cell were studied;and it was found that with the increase of cell concentration,hepatic cell showed fluorescence saturation. The experimental results with a theoretical basis for the in-depth study of hepatic cell was provided and the foundation for the subsequent comparative study of liver and liver cancer cells was laid.
In order to overcome the need for liver cells to understand liver cancer,the difference can be compared between liver cells and liver cancer cells, and the screening of liver cancer can be achieved early. Firstly, HL-7702[L-02] hepatic cell was cultured in the laboratory,and then the autofluorescence spectrum of hepatic cell were studied by fluorescence spectrometry. The results showed that the fluorescence spectrum of hepatic cell showed two distinct peaks at 588nm and 660nm,respectively,and the maximum fluorescence intensity was obtained at 588nm. Then,the morphology of the hepatic cell was observed by fluorescence microscopy,and the fluorescence photographs of the cells in multiple fields of view were calculated,and the average diameter of the hepatic cell was obtained to be close to 13.1μm. The experiment of flow cytometer shows that the diameter of hepatic cell was relatively concentrated. Finally,the changes of fluorescence saturation intensity of hepatic cell were studied;and it was found that with the increase of cell concentration,hepatic cell showed fluorescence saturation. The experimental results with a theoretical basis for the in-depth study of hepatic cell was provided and the foundation for the subsequent comparative study of liver and liver cancer cells was laid.
引文
[1]李静,曾红娟,庞小峰.肝癌细胞和正常肝细胞的自体荧光光谱研究[J].光学学报,2009,29(8):2261-2263.
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[10]杜恩辅,徐霖,周选民,等.荧光探针偶联GPC3单链抗体对肝癌的体内外检测研究[J].检验医学与临床,2018,15(11):1583-1586.
[11]吴拥军,郝艳红,吴维超,等.血清自体荧光光谱联合肿瘤标志物群在肺癌诊断中的价值[J].光谱学与光谱分析,2009,29(10):2787-2791.
[12]申炳俊,苗苗,刘慧,等.荧光光谱法研究柯里拉京与人血清白蛋白之间的相互作用[J].长春理工大学学报:自然科学版,2016,39(6):75-80.
[2]董小玉,刘秀敏,刘张苑珠,等.胃肠道肿瘤的多光子成像与光学活检[J].分子影像学杂志,2017,40(3):344-349.
[3]蒋宏图,刘适搏,孙媛媛.分子荧光光度法测定水中石油类物质[J].长春理工大学学报:自然科学版,2010,33(4):168-173.
[4]陶春先,阮俊,舒顺朋,等.三维荧光光谱对食用植物油中煎炸油的检测[J].中国激光,2016,43(1):219-225.
[5]汤陌生.自体荧光成像技术在乳腺癌和结肠癌诊断中的应用[J].中国医疗设备,2014,29(4):33-35.
[6]吴志伟,林居强,黄义梅,等.鼻咽癌增殖与转移机制荧光检测的研究进展[J].光电子·激光,2018,29(8):917-924.
[7]李晓舟,田湘湘,杨天月.肺癌的血清自体荧光检测[J].光散射学报,2011,23(4):402-405.
[8]裴娇娇,常淑芳,孙江川.光谱技术在宫颈上皮内瘤变监测中的研究进展[J].激光杂志,2014,35(5):59-61.
[9]Dan C,Yue P,Lu W,et al.Selective Visualization of the endogenous peroxynitrite in an inflamed mouse model by a mitochondria-targetable two-photon ratiometric fluorescent probe[J].Journal of the American Chemical Society,2017,139(1):285-292
[10]杜恩辅,徐霖,周选民,等.荧光探针偶联GPC3单链抗体对肝癌的体内外检测研究[J].检验医学与临床,2018,15(11):1583-1586.
[11]吴拥军,郝艳红,吴维超,等.血清自体荧光光谱联合肿瘤标志物群在肺癌诊断中的价值[J].光谱学与光谱分析,2009,29(10):2787-2791.
[12]申炳俊,苗苗,刘慧,等.荧光光谱法研究柯里拉京与人血清白蛋白之间的相互作用[J].长春理工大学学报:自然科学版,2016,39(6):75-80.