玉米核雄性不育的分子机制研究与应用分析
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摘要
玉米细胞核雄性不育突变体是研究花粉发育和减数分裂的理想材料,也是杂种优势利用的重要种质资源。随着分子生物技术的快速发展,部分玉米细胞核雄性不育基因陆续被成功克隆,为其在工程不育化杂交种生产中的应用奠定了基础。综述了近年来对玉米细胞核雄性不育的细胞学鉴定、基因克隆和分子机制的研究进展,并对其应用途径和前景进行了分析。
Maize( Zea mays L.) genic male-sterile mutant has been widely studied for its biological significance on pollen developmental study and commercial value in hybrid vigor utilization. As the development of molecular biotechnology,several genic male-sterile genes has been cloned and characterized in maize,leading to potential use of genetic engineering male-sterility in commercial hybrid seed production. Here,the progress of cytological characterization,gene cloning and molecular mechanism of genic male-sterility study in maize are reviewe,and potential application approaches of genic male-sterile genes in maize hybrid production is discussed.
Maize( Zea mays L.) genic male-sterile mutant has been widely studied for its biological significance on pollen developmental study and commercial value in hybrid vigor utilization. As the development of molecular biotechnology,several genic male-sterile genes has been cloned and characterized in maize,leading to potential use of genetic engineering male-sterility in commercial hybrid seed production. Here,the progress of cytological characterization,gene cloning and molecular mechanism of genic male-sterility study in maize are reviewe,and potential application approaches of genic male-sterile genes in maize hybrid production is discussed.
引文
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[2]Skibbe D S,Schnable P S.Male sterility in maize.Maydica,2005,50:367-376.
[3]Chen X,Zhang H,Sun H,et al.IRREGULAR POLLEN EXINE1 is a novel factor in anther cuticle and pollen exine formation.Plant Physiol,2017,173(1):307-325.
[4]Somaratne Y,Tian Y,Zhang H,et al.ABNORMAL POLLEN VACUOLATION1(APV1)is required for male fertility by contributing to anther cuticle and pollen exine formation in maize.Plant J,2017,90(1):96-110.
[5]Albertsen M C,Phillips R L.Developmental cytology of 13genetic male sterile loci in maize.Can J Genet Cytol,1981,23:195-208.
[6]万向元,吴锁伟,周岩,等.植物花粉发育调控基因Ms1及其编码蛋白:中国,ZL201410381072.5.2017-06-16.[2017-08-19].http://cpquery.sipo.gov.cn/txn Pantent Info List.do?inner-flag:open-type=window&inner-flag:flowno=1515028304864.Wan X Y,Wu S W,Zhou Y,et al.The DNA Sequence and the Coded protein of Ms1 Gene Which Controls the Male Fertility of Plants.Chinese,ZL201410381072.5.2017-06-16.[2017-08-19].http://cpquery.sipo.gov.cn/txn Pantent Info List.do?inner-flag:open-type=window&inner-flag:flowno=1515028304864.
[7]Eyster L A.Heritable characters of maize.VII.Male sterile.Journal of Heredity,1921,12(3):138-141.
[8]Eyster W H.Heritable characters of maize XXXIX-Male sterile3.Journal of Heredity,1931,22(4):117-119.
[9]Beadle G W.Genes in maize for pollen sterility.Genetics,1932,17:413-431.
[10]Morton C M,Lawson D L,Bedinger P.Morphological study of the maize male sterile mutant ms7.Maydica,1989,34:239-245.
[11]Zhang D,Wu S,An X,et al.Construction of a multi-control sterility system for a maize male-sterile line and hybrid seed production based on the Zm Ms7 gene encoding a PHD-finger transcription factor.Plant Biotechnol J.[2017-02-01].https://doi.org/10.1111/pbi.12786.
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[13]Wang D,Oses-Prieto J A,Li K H,et al.The male sterile 8mutation of maize disrupts the temporal progression of the transcriptome and results in the mis-regulation of metabolic functions.Plant J,2010,63(6):939-951.
[14]Wang D,Skibbe DS and Walbot V.Maize Male sterile 8(Ms8),a putativeβ-1,3-galactosyltransferase,modulates cell division,expansion,and differentiation during early maize anther development.Plant Reprod,2013,26(4):329-338.
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[22]Nan G L,Zhai J,Arikit S,et al.MS23,a master basic helixloop-helix factor,regulates the specification and development of the tapetum in maize.Development,2017,144(1):163-172.
[23]Fox T,Trimnell M R and Albertsen M C.Male-sterile mutant ms24 mapped to chormosome 10.Maize Genet Coop Newsletter,2002,76:37.
[24]Djukanovic V,Smith J,Lowe K,et al.Male-sterile maize plants produced by targeted mutagenesis of the cytochrome P450-like gene(MS26)using a re-designed I-Cre I homing endonuclease.Plant J,2013,76(5):888-899.
[25]Loukides C A,Broadwater A H,Bedinger P A.Two new malesterile mutants of Zea mays(Poaceae)with abnormal tapetal cell morphology.American Journal of Botany,1995,87(8):1017-1023.
[26]Golubovskaya I N.Mapping of two mei-genes of maize wtih the help of B-A translocations.Soviet Genetics,1987,23:473-480.
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[31]Trimnell M R,Patterson E,Fox T W,et al.New chromosome 2L male-sterile mutant:ms33 and alleles.Maize Genet Coop Newsletter,1999,73:48.
[32]万向元,吴锁伟,张丹凤,等.玉米花粉发育调控基因Ms33的DNA序列及其编码蛋白:中国,CN201610880590.0.2017-02-15.[2017-08-19].http://cpquery.sipo.gov.cn/txn Pantent Info List.do?inner-flag:open-type=window&innerflag:flowno=1515028304864.Wan X Y,Wu S W,Zhang D F,et al.The DNA Sequence and the Coded protein of a Pollen Developmental Gene Ms33 in Maize.China,CN201610880590.0.2017-02-15.[2017-08-19].http://cpquery.sipo.gov.cn/txn Pantent Info List.do?inner-flag:open-type=window&inner-flag:flowno=1515028304864.
[33]Trimnell M R,Patterson E,Fox T W,et al.New chromosome 7L male-sterile mutant:ms34.Maize Genet Coop Newsletter,1999,73:49.
[34]Trimnell M R,Patterson E,Fox T W,et al.New chromosome 9L male-sterile mutants:ms35 and ms36.Maize Genet Coop Newsletter,1999,73:49.
[35]Trimnell M R,Fox T W,Albertsen M C.New chromosome 7L male-sterile mutant:ms37.Maize Genet Coop Newsletter,1999,73:48.
[36]Albertsen M C,Fox T W,Trimnell M R.Changing a duplicated designation for two different male-sterile mutations.Maize Genet Coop Newsletter,1999,73:48.
[37]Albertsen M C,Sellner L M.An independent,EMS-induced dominant male sterile that maps similar to Ms41.Maize Genet Coop Newsletter,1988,62:70.
[38]Albertsen M C,Fox T W,Trimnell M R,et al.Interval mapping a new dominant male-sterile mutant,Ms42.Maize Genet Coop Newsletter,1993,67:64.
[39]Fox T,De Bruin J,Haug Collet K,et al.A single point mutation in Ms44 results in dominant male sterility and improves nitrogen use efficiency in maize.Plant Biotechnol J,2017,15(8):942-952.
[40]Cigan A M,Unger E,Xu R J,et al.,Phenotypic complementation of ms45 maize requires tapetal expression of MS45.Sex Plant Reprod,2001,14(3):135-142.
[41]Trimnell M R,Fox T W,Albertsen M C.New chromosome 10male-sterile mutant:ms47.Maize Genet Coop Newsletter,2002,76:38.
[42]Trimnell M R,Fox T W,Albertsen M C.New chromosome 9L male-sterile mutant:ms48.Maize Genet Coop Newsletter,2002,76:38.
[43]Trimnell M R,Fox T W,Albertsen M C.New chromosome 10male-sterile mutant:ms49.Maize Genet Coop Newsletter,2002,76:38-39.
[44]Trimnell M R,Fox T W,Albertsen M C.New chromosome 6L male-sterile mutant:ms50.Maize Genet Coop Newsletter,2002,76:39.
[45]Trimnell M R,Fox T W,Albertsen M C.New chromosome 4male-sterile mutant:ms52.Maize Genet Coop Newsletter,2004,78:27.
[46]Vernoud V,Laigle G,Rozier F,et al.The HD-ZIP IV transcription factor OCL4 is necessary for trichome patterning and anther development in maize.Plant J,2009,59(6):883-894.
[47]Wang C J,Nan G L,Kelliher T,et al.Maize multiple archesporial cells 1(mac1),an ortholog of rice TDL1A,modulates cell proliferation and identity in early anther development.Development,2012,139(14):2594-2603.
[48]Wang D,Skibbe D S,and Walbot V.Maize csmd1 exhibits premeiotic somatic and post-meiotic microspore and somatic defects but sustains anther growth.Sex Plant Reprod,2011,24(4):297-306.
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[2]Skibbe D S,Schnable P S.Male sterility in maize.Maydica,2005,50:367-376.
[3]Chen X,Zhang H,Sun H,et al.IRREGULAR POLLEN EXINE1 is a novel factor in anther cuticle and pollen exine formation.Plant Physiol,2017,173(1):307-325.
[4]Somaratne Y,Tian Y,Zhang H,et al.ABNORMAL POLLEN VACUOLATION1(APV1)is required for male fertility by contributing to anther cuticle and pollen exine formation in maize.Plant J,2017,90(1):96-110.
[5]Albertsen M C,Phillips R L.Developmental cytology of 13genetic male sterile loci in maize.Can J Genet Cytol,1981,23:195-208.
[6]万向元,吴锁伟,周岩,等.植物花粉发育调控基因Ms1及其编码蛋白:中国,ZL201410381072.5.2017-06-16.[2017-08-19].http://cpquery.sipo.gov.cn/txn Pantent Info List.do?inner-flag:open-type=window&inner-flag:flowno=1515028304864.Wan X Y,Wu S W,Zhou Y,et al.The DNA Sequence and the Coded protein of Ms1 Gene Which Controls the Male Fertility of Plants.Chinese,ZL201410381072.5.2017-06-16.[2017-08-19].http://cpquery.sipo.gov.cn/txn Pantent Info List.do?inner-flag:open-type=window&inner-flag:flowno=1515028304864.
[7]Eyster L A.Heritable characters of maize.VII.Male sterile.Journal of Heredity,1921,12(3):138-141.
[8]Eyster W H.Heritable characters of maize XXXIX-Male sterile3.Journal of Heredity,1931,22(4):117-119.
[9]Beadle G W.Genes in maize for pollen sterility.Genetics,1932,17:413-431.
[10]Morton C M,Lawson D L,Bedinger P.Morphological study of the maize male sterile mutant ms7.Maydica,1989,34:239-245.
[11]Zhang D,Wu S,An X,et al.Construction of a multi-control sterility system for a maize male-sterile line and hybrid seed production based on the Zm Ms7 gene encoding a PHD-finger transcription factor.Plant Biotechnol J.[2017-02-01].https://doi.org/10.1111/pbi.12786.
[12]Wang D,Adams C M,Fernandes J F,et al.A low molecular weight proteome comparison of fertile and male sterile 8 anthers of Zea mays.Plant Biotechnol J,2012,10(8):925-935.
[13]Wang D,Oses-Prieto J A,Li K H,et al.The male sterile 8mutation of maize disrupts the temporal progression of the transcriptome and results in the mis-regulation of metabolic functions.Plant J,2010,63(6):939-951.
[14]Wang D,Skibbe DS and Walbot V.Maize Male sterile 8(Ms8),a putativeβ-1,3-galactosyltransferase,modulates cell division,expansion,and differentiation during early maize anther development.Plant Reprod,2013,26(4):329-338.
[15]Albertsen M,Fox T,Leonard A,et al.Cloning and Use of the ms9 Gene From Maize:US,US20160024520A1.2016-01-28.[2017-08-19].http://appft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&co1=AND&d=PG01&s1=ms9.TTL.&OS=TTL/ms9&RS=TTL/ms9.
[16]Kelliher T,Egger R L,Zhang H,et al.Unresolved issues in premeiotic anther development.Front Plant Sci,2014,5:347.
[17]余自青,吴锁伟,张丹凤,等.玉米隐性核不育突变体ms14的遗传分析与基因定位.中国生物工程杂志,2016,36(10):8-14.Yu Z Q,Wu S W,Zhang D F,et al.Genetic analysis and gene mapping of recessive genic male sterility14(ms14)mutant in maize.China Biotechnology,2016,36(10):8-14.
[18]Eyster W H.Genetics of Zea mays.Bibliographia Genetica,1934,11:187-192.
[19]Schwartz D.The interaction of nuclear and cytoplasmic factors in the inheritance of male sterility in maize.Genetics,1951,36(6):676-696.
[20]Albertsen M C,Fox T,Trimnell M,et al.Msca1 Nucleotide Sequences Impacting Plant Male Fertility and Method of Using Same:US,US20090038027A1.2009-02-05.[2017-08-19].http://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&co1=AND&d=PTXT&s1=7910802.PN.&OS=PN/7910802&RS=PN/7910802.
[21]Chaubal R,Anderson J R,Trimnell M R,et al.The transformation of anthers in the msca1 mutant of maize.Planta,2003,216(5):778-788.
[22]Nan G L,Zhai J,Arikit S,et al.MS23,a master basic helixloop-helix factor,regulates the specification and development of the tapetum in maize.Development,2017,144(1):163-172.
[23]Fox T,Trimnell M R and Albertsen M C.Male-sterile mutant ms24 mapped to chormosome 10.Maize Genet Coop Newsletter,2002,76:37.
[24]Djukanovic V,Smith J,Lowe K,et al.Male-sterile maize plants produced by targeted mutagenesis of the cytochrome P450-like gene(MS26)using a re-designed I-Cre I homing endonuclease.Plant J,2013,76(5):888-899.
[25]Loukides C A,Broadwater A H,Bedinger P A.Two new malesterile mutants of Zea mays(Poaceae)with abnormal tapetal cell morphology.American Journal of Botany,1995,87(8):1017-1023.
[26]Golubovskaya I N.Mapping of two mei-genes of maize wtih the help of B-A translocations.Soviet Genetics,1987,23:473-480.
[27]Trimnell M R,Fox T W,Albertsen M C.New chromosome 10S male-sterile mutant:ms29.Maize Genet Coop Newsletter,1998,72:37.
[28]Trimnell M R,Fox T W,Albertsen M C.New chromosome 2L male-sterile mutants:ms30 and ms31.Maize Genet Coop Newsletter,1998,72:38.
[29]万向元,吴锁伟,周岩,等.一种玉米花粉减数分裂后发育调控基因Ms30的DNA序列及其编码蛋白,中国,ZL201410703778.9.2017-04-12.[2017-08-19].http://cpquery.sipo.gov.cn/txn Pantent Info List.do?inner-flag:opentype=window&inner-flag:flowno=1515028304864.Wan X Y,Wu S W,Xie K,et al.The DNA Sequence and the Coded Protein of a Pollen Post-meiotic Developmental Gene Ms30in Maize:China,ZL201410703778.9.2017-04-12.[2017-08-19].http://cpquery.sipo.gov.cn/txn Pantent Info List.do?inner-flag:open-type=window&inner-flag:flowno=1515028304864.
[30]Moon J,Skibbe D,Timofejeva L,et al.Regulation of cell divisions and differentiation by MALE STERILITY32 is required for anther development in maize.Plant J,2013,76(4):592-602.
[31]Trimnell M R,Patterson E,Fox T W,et al.New chromosome 2L male-sterile mutant:ms33 and alleles.Maize Genet Coop Newsletter,1999,73:48.
[32]万向元,吴锁伟,张丹凤,等.玉米花粉发育调控基因Ms33的DNA序列及其编码蛋白:中国,CN201610880590.0.2017-02-15.[2017-08-19].http://cpquery.sipo.gov.cn/txn Pantent Info List.do?inner-flag:open-type=window&innerflag:flowno=1515028304864.Wan X Y,Wu S W,Zhang D F,et al.The DNA Sequence and the Coded protein of a Pollen Developmental Gene Ms33 in Maize.China,CN201610880590.0.2017-02-15.[2017-08-19].http://cpquery.sipo.gov.cn/txn Pantent Info List.do?inner-flag:open-type=window&inner-flag:flowno=1515028304864.
[33]Trimnell M R,Patterson E,Fox T W,et al.New chromosome 7L male-sterile mutant:ms34.Maize Genet Coop Newsletter,1999,73:49.
[34]Trimnell M R,Patterson E,Fox T W,et al.New chromosome 9L male-sterile mutants:ms35 and ms36.Maize Genet Coop Newsletter,1999,73:49.
[35]Trimnell M R,Fox T W,Albertsen M C.New chromosome 7L male-sterile mutant:ms37.Maize Genet Coop Newsletter,1999,73:48.
[36]Albertsen M C,Fox T W,Trimnell M R.Changing a duplicated designation for two different male-sterile mutations.Maize Genet Coop Newsletter,1999,73:48.
[37]Albertsen M C,Sellner L M.An independent,EMS-induced dominant male sterile that maps similar to Ms41.Maize Genet Coop Newsletter,1988,62:70.
[38]Albertsen M C,Fox T W,Trimnell M R,et al.Interval mapping a new dominant male-sterile mutant,Ms42.Maize Genet Coop Newsletter,1993,67:64.
[39]Fox T,De Bruin J,Haug Collet K,et al.A single point mutation in Ms44 results in dominant male sterility and improves nitrogen use efficiency in maize.Plant Biotechnol J,2017,15(8):942-952.
[40]Cigan A M,Unger E,Xu R J,et al.,Phenotypic complementation of ms45 maize requires tapetal expression of MS45.Sex Plant Reprod,2001,14(3):135-142.
[41]Trimnell M R,Fox T W,Albertsen M C.New chromosome 10male-sterile mutant:ms47.Maize Genet Coop Newsletter,2002,76:38.
[42]Trimnell M R,Fox T W,Albertsen M C.New chromosome 9L male-sterile mutant:ms48.Maize Genet Coop Newsletter,2002,76:38.
[43]Trimnell M R,Fox T W,Albertsen M C.New chromosome 10male-sterile mutant:ms49.Maize Genet Coop Newsletter,2002,76:38-39.
[44]Trimnell M R,Fox T W,Albertsen M C.New chromosome 6L male-sterile mutant:ms50.Maize Genet Coop Newsletter,2002,76:39.
[45]Trimnell M R,Fox T W,Albertsen M C.New chromosome 4male-sterile mutant:ms52.Maize Genet Coop Newsletter,2004,78:27.
[46]Vernoud V,Laigle G,Rozier F,et al.The HD-ZIP IV transcription factor OCL4 is necessary for trichome patterning and anther development in maize.Plant J,2009,59(6):883-894.
[47]Wang C J,Nan G L,Kelliher T,et al.Maize multiple archesporial cells 1(mac1),an ortholog of rice TDL1A,modulates cell proliferation and identity in early anther development.Development,2012,139(14):2594-2603.
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