玉米细胞核雄性不育突变体K305ms的生理生化分析
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摘要
为探讨60Co-γ辐射诱变获得的玉米细胞核雄性不育突变体K305ms的生理生化特征,以不育株K305ms及其姊妹交群体中的可育株K305F为材料,对雄花发育不同时期花药和颖壳中的可溶性蛋白含量、游离脯氨酸含量及超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性进行了测定。结果表明,与可育株K305F相比,不育株K305ms雄花发育过程中花药与颖壳的可溶性蛋白含量和游离脯氨酸含量均不同程度降低,且K305ms花药的SOD和CAT活性明显降低,而POD活性显著或极显著升高。说明玉米细胞核雄性不育可能与花药发育过程中物质代谢和抗氧化系统异常有关。
In order to understand physiological and biochemical characteristics of the maize genic male sterile mutant K305 ms induced by60 Co-γ irradiation,male sterile mutant K305 ms and fertile plant K305 F from their sister-crossed line were used as materials. The content change of soluble protein,free proline,and the activity of superoxide dismutase(SOD),catalase(CAT),peroxidase(POD) during the anther development stage were detected. The main results were showed as follows: The soluble protein content and free proline content in anther and glume of K305 ms mutant were lower than fertile plant K305 F during anther development stage; Moreover,the activities of SOD and CAT in anther of K305 ms mutant were significant lower than those in K305 F,whereas the POD activity was significant higher. The results indicated that genic male sterility of maize was related to abnormal metabolism of soluble protein and the disordered reactive oxygen scavenging enzyme system(ROS) during the development of anther.
In order to understand physiological and biochemical characteristics of the maize genic male sterile mutant K305 ms induced by60 Co-γ irradiation,male sterile mutant K305 ms and fertile plant K305 F from their sister-crossed line were used as materials. The content change of soluble protein,free proline,and the activity of superoxide dismutase(SOD),catalase(CAT),peroxidase(POD) during the anther development stage were detected. The main results were showed as follows: The soluble protein content and free proline content in anther and glume of K305 ms mutant were lower than fertile plant K305 F during anther development stage; Moreover,the activities of SOD and CAT in anther of K305 ms mutant were significant lower than those in K305 F,whereas the POD activity was significant higher. The results indicated that genic male sterility of maize was related to abnormal metabolism of soluble protein and the disordered reactive oxygen scavenging enzyme system(ROS) during the development of anther.
引文
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[15]刘金兵,侯喜林,王述彬,等.甜椒胞质雄性不育(CMS)系及其保持系花药中游离氨基酸含量[J].江苏农业学报,2006,22(1):68-70.LIU J B,HOU X L,WANG S B,et al.Free amono acids of anther in sweet pepper CMS lines and their maintainer lines[J].Jiangsu Journal of Agricultural Science,2006,22(1):68-70.(in Chinese with English abstract)
[16]梁艳荣,胡小红,陈源闽,等.胡萝卜雄性不育系生理生化特性研究[J].华北农学报,2006,21(3):19-22.LIANG Y S,HU X H,CHEN Y M,et al.Studies on the physiological and biochemical characters in carrot male-sterile lines[J].Acta Agriculturae Boreali-Sinica,2006,21(3):19-22.(in Chinese with English abstract)
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[18]李巍,马翎健,何蓓如.两类小麦温敏雄性不育系育性敏感时期生理生化指标的变化[J].麦类作物学报,2009,29(1):89-92.LI W,MA L J,HE B R.Change of some physiological characters in the fertility sensitive period of two types of temperature sensitive male sterile wheat lines[J].Journal of Triticeae Crops,2009,29(1):89-92.(in Chinese with English abstract)
[19]王先裕,梁聪耀,邓廖芬,等.番茄温敏雄性不育系T-4生理生化特性研究[J].北方园艺,2011(15):29-31.WANG X Y,LIANG C Y,DENG L F,et al.Studies on physiological and biochemical characters in thermo-sensitive male sterile line tomato Solanum lycopersicum L.T-4[J].Northern Horticulture,2011(15):29-31.(in Chinese with English abstract)
[20]LEHMANN S,FUNCK D,SZABADOS L,et al.Proline metabolism and transport in plant development[J].Amino Acids,2010,39(4):949-962.
[21]MITTLER R.Oxidative stress,antioxidants and stress tolerance[J].Trends in Plant Science,2002,7(9):405-410.
[22]BATINIC-HABERLE I,TOVMASYAN A,ROBERTS E R,et al.SOD therapeutics:latest insights into their structureactivity relationships and impact on the cellular redox-based signaling pathways[J].Antioxidants&Redox Signaling,2014,20(15):2372-2415.
[23]O'BRIEN J A,DAUDI A,BUTT V S,et al.Reactive oxygen species and their role in plant defence and cell wall metabolism[J].Planta,2012,236(3):765-779.
[24]SUZUKI N,KOUSSEVITZKY S,MITTLER R,et al.ROS and redox signalling in the response of plants to abiotic stress[J].Plant,Cell&Environment,2012,35(2):259-270.
[25]HASANUZZAMAN M,HOSSAIN M A,DA SILVA J A T,et al.Plant response and tolerance to abiotic oxidative stress:antioxidant defense is a key factor[M]//VENKATESWARLU B,SHANKER AK,SHANKER C,et al.Crop stress and its perspectives and strategies.Dordrecht:Springer,2012:261-315.
[26]伊风艳,石凤翎,高翠萍,等.苜蓿雄性不育株与可育株生理生化特性的比较[J].中国草地学报,2014,36(6):60-65.YI F Y,SHI F L,GAO C P,et al.A comparative analysis of physiological and biochemical characteristic between alfalfa male sterile plants and male fertile plants[J].Chinese Journal of Grassland,2014,36(6):60-65.(in Chinese with English abstract)
[27]张克中,赵祥云,黄善武,等.王百合雄性不育突变体无性系花蕾花药的生化分析[J].东北林业大学学报,2003,31(3):31-33.ZHANG K Z,ZHAO X Y,HUANG S W,et al.Biochemical analysis of flower buds and anther in Lilium regale's male sterility mutants lines[J].Jounal of Northeast Forestry University,2003,31(3):31-33.(in Chinese with English abstract)
[28]SPENA A,ESTRUCH J J,PRINSEN E,et al.Anther-specific expression of the rol B gene of Agrobacterium rhizogenes increases IAA content in anthers and alters anther development and whole flower growth[J].Theoretical and Applied Genetics,1992,84(5/6):520-527.
[29]赵前程,耿宵,陈雪平,等.花椰菜雄性不育系小孢子发育过程及其POD活性[J].华北农学报,2002,17(2):108-111.ZHAO Q C,GENG X,CHEN X P,et al.Cytological observation on microsporogenesis of male sterile line of cauliflower[J].Acta Agriculturae Boreali-Sinica,2002,17(2):108-111.(in Chinese with English abstract)
[30]张子学,侯喜林.辣椒细胞质雄性不育与活性氧代谢的关系[J].西北植物学报,2005,25(4):799-802.ZHANG Z X,HOU X L.Relation between cytoplasmic male sterility and reactive oxygen species metabolism in pepper[J].Acta Botanica Boreali-Occidentalia Sinica,2005,25(4):799-802.(in Chinese with English abstract)
[31]张建奎,宗学凤,王俊义,等.温光敏核雄性不育小麦花药中保护酶活性的变化[J].麦类作物学报,2001,21(4):26-30.ZHANG J K,ZONG X F,WANG J Y,et al.Changes of activity of protective enzymes in anther of thermo-photo-sensitive genic male-sterile wheat[J].Journal of Triticeae Crops,2001,21(4):26-30.(in Chinese with English abstract)
[32]石晓艺,胡甘,蒙立颖,等.5种细胞质雄性不育小麦败育过程中活性氧代谢保护酶的响应[J].麦类作物学报,2014,34(12):1656-1660.SHI X Y,HU G,MENG L Y,et al.Activities of protective enzymes of reactive oxygen metabolism during aborting process of 5 kinds of cytoplasmic male wheat lines[J].Journal of Triticeae Crops,2014,34(12):1656-1660.(in Chinese with English abstract)
[33]刘莉.水稻细胞质雄性不育系和保持系表型观察及活性氧代谢研究[D].南京:南京师范大学,2016.LIU L.Studies on the phenotype observation and reactive oxygen metabolism of cytoplamsic male sterility and their maintainer lines in rice[D].Nanjing:Nanjing Normal University,2016.
[2]胡丽芳,苏连水,朱昌兰,等.辐射诱变水稻雄性不育突变体tda的遗传与细胞学分析[J].核农学报,2015,29(12):2253-2258.HU L F,SU L S,ZHU C L,et al.Genetic and cytological analysis of a rice male sterile mutant tda derived from radiation mutation[J].Journal of Nuclear Agricultural Sciences,2015,29(12):2253-2258.(in Chinese with English abstract)
[3]WANG Y,GU R,CHEN H,et al.Characterization and genetic mapping of a novel recessive genic male sterile gene ms305 in maize(Zea mays L.)[J].Israel Journal of Plant Sciences,2015,62(3):208-214.
[4]陈华伟,石海春,余学杰,等.1份60Co-γ射线诱变玉米雄性不育突变体的遗传分析[J].核农学报,2016,30(5):829-834.CHEN H W,SHI H C,YU X J,et al.Genetic analysis of a maize male sterile mutant induced by60Co-γirradiation[J].Journal of Nuclear Agricultural Sciences,2016,30(5):829-834.(in Chinese with English abstract)
[5]石海春,粟佳美,赵长云,等.玉米核不育突变体K305ms主要性状配合力及产量杂种优势研究[J].核农学报,2016,30(4):621-628.SHI H C,SU J M,ZHAO C Y,et al.Combining ability of the major characters and yield heterosis analysis of geneic male sterility mutant K305ms in maize[J].Journal of Nuclear Agricultural Sciences,2016,30(4):621-628.(in Chinese with English abstract)
[6]BLAKESLEY R W,BOEZI J A.A new staining technique for proteins in polyacrylamide gels using Coomassie Brilliant Blue G250[J].Analytical Biochemistry,1977(82):580-582.
[7]张宪政,谭桂茹.植物生理学实验技术[M].沈阳:辽宁科学技术出版社,1989.
[8]邹佳,蔺万煌,罗红兵,等.玉米C型胞质雄性不育与可溶性糖,可溶性蛋白,游离脯氨酸含量的关系[J].湖南农业大学学报(自然科学版).2009,35(3):249-251.ZOU J,LIN W H,LUO H B,et al.Relationship between contents of soluble sugar,soluble protein and free proline and C-cytoplasmic male sterility in maize[J].Journal of Hunan Agricultural University(Natural Sciences),2009,35(3):249-251.(in Chinese with English abstract)
[9]朱广廉,曹宗巽.花粉中的游离脯氨酸及其生理功能[J].植物生理学通讯,1985(4):9-14.ZHU G L,CAO Z X.Free proline and its physiological functions in pollen[J].Plant Physiology Communications,1985(4):9-14.(in Chinese)
[10]SZABADOS L,SAVOURA.Proline:a multifunctional amino acid[J].Trends in Plant Science,2010,15(2):89-97.
[11]MATTIOLI R,COSTANTINO P,TROVATO M.Proline accumulation in plants:not only stress[J].Plant Signaling&Behavior,2009,4(11):1016-1018.
[12]CHIANG H H,DANDEKAR A M.Regulation of proline accumulation in Arabidopsis thaliana(L.)Heynh during development and in response to desiccation[J].Plant,Cell&Environment,1995,18(11):1280-1290.
[13]SCHWACKE R,GRALLATH S,BREITKREUZ K E,et al.Le Pro T1,a transporter for proline,glycine betaine,andγ-amino butyric acid in tomato pollen[J].Plant Cell,1999,11(3):377-391.
[14]STINES A P,NAYLOR D J,HJ P B,et al.Proline accumulation in developing grapevine fruit occurs independently of changes in the levels ofΔ1-pyrroline-5-carboxylate synthetase m RNA or protein[J].Plant Physiology,1999,120(3):923-931.
[15]刘金兵,侯喜林,王述彬,等.甜椒胞质雄性不育(CMS)系及其保持系花药中游离氨基酸含量[J].江苏农业学报,2006,22(1):68-70.LIU J B,HOU X L,WANG S B,et al.Free amono acids of anther in sweet pepper CMS lines and their maintainer lines[J].Jiangsu Journal of Agricultural Science,2006,22(1):68-70.(in Chinese with English abstract)
[16]梁艳荣,胡小红,陈源闽,等.胡萝卜雄性不育系生理生化特性研究[J].华北农学报,2006,21(3):19-22.LIANG Y S,HU X H,CHEN Y M,et al.Studies on the physiological and biochemical characters in carrot male-sterile lines[J].Acta Agriculturae Boreali-Sinica,2006,21(3):19-22.(in Chinese with English abstract)
[17]邓明华,周群初,刘志敏,等.辣椒细胞质雄性不育系与保持系生化特性研究[J].湖南农业大学学报(自然科学版),2002,28(6):492-494.DENG M H,ZHOU Q C,LIU Z M,et al.A study on some biochemical characters in cytoplasmic male-sterile lines and their maintainer lines of hot pepper[J].Journal of Hunan Agricultural University(Natural Sciences),2002,28(6):492-494.(in Chinese with English abstract)
[18]李巍,马翎健,何蓓如.两类小麦温敏雄性不育系育性敏感时期生理生化指标的变化[J].麦类作物学报,2009,29(1):89-92.LI W,MA L J,HE B R.Change of some physiological characters in the fertility sensitive period of two types of temperature sensitive male sterile wheat lines[J].Journal of Triticeae Crops,2009,29(1):89-92.(in Chinese with English abstract)
[19]王先裕,梁聪耀,邓廖芬,等.番茄温敏雄性不育系T-4生理生化特性研究[J].北方园艺,2011(15):29-31.WANG X Y,LIANG C Y,DENG L F,et al.Studies on physiological and biochemical characters in thermo-sensitive male sterile line tomato Solanum lycopersicum L.T-4[J].Northern Horticulture,2011(15):29-31.(in Chinese with English abstract)
[20]LEHMANN S,FUNCK D,SZABADOS L,et al.Proline metabolism and transport in plant development[J].Amino Acids,2010,39(4):949-962.
[21]MITTLER R.Oxidative stress,antioxidants and stress tolerance[J].Trends in Plant Science,2002,7(9):405-410.
[22]BATINIC-HABERLE I,TOVMASYAN A,ROBERTS E R,et al.SOD therapeutics:latest insights into their structureactivity relationships and impact on the cellular redox-based signaling pathways[J].Antioxidants&Redox Signaling,2014,20(15):2372-2415.
[23]O'BRIEN J A,DAUDI A,BUTT V S,et al.Reactive oxygen species and their role in plant defence and cell wall metabolism[J].Planta,2012,236(3):765-779.
[24]SUZUKI N,KOUSSEVITZKY S,MITTLER R,et al.ROS and redox signalling in the response of plants to abiotic stress[J].Plant,Cell&Environment,2012,35(2):259-270.
[25]HASANUZZAMAN M,HOSSAIN M A,DA SILVA J A T,et al.Plant response and tolerance to abiotic oxidative stress:antioxidant defense is a key factor[M]//VENKATESWARLU B,SHANKER AK,SHANKER C,et al.Crop stress and its perspectives and strategies.Dordrecht:Springer,2012:261-315.
[26]伊风艳,石凤翎,高翠萍,等.苜蓿雄性不育株与可育株生理生化特性的比较[J].中国草地学报,2014,36(6):60-65.YI F Y,SHI F L,GAO C P,et al.A comparative analysis of physiological and biochemical characteristic between alfalfa male sterile plants and male fertile plants[J].Chinese Journal of Grassland,2014,36(6):60-65.(in Chinese with English abstract)
[27]张克中,赵祥云,黄善武,等.王百合雄性不育突变体无性系花蕾花药的生化分析[J].东北林业大学学报,2003,31(3):31-33.ZHANG K Z,ZHAO X Y,HUANG S W,et al.Biochemical analysis of flower buds and anther in Lilium regale's male sterility mutants lines[J].Jounal of Northeast Forestry University,2003,31(3):31-33.(in Chinese with English abstract)
[28]SPENA A,ESTRUCH J J,PRINSEN E,et al.Anther-specific expression of the rol B gene of Agrobacterium rhizogenes increases IAA content in anthers and alters anther development and whole flower growth[J].Theoretical and Applied Genetics,1992,84(5/6):520-527.
[29]赵前程,耿宵,陈雪平,等.花椰菜雄性不育系小孢子发育过程及其POD活性[J].华北农学报,2002,17(2):108-111.ZHAO Q C,GENG X,CHEN X P,et al.Cytological observation on microsporogenesis of male sterile line of cauliflower[J].Acta Agriculturae Boreali-Sinica,2002,17(2):108-111.(in Chinese with English abstract)
[30]张子学,侯喜林.辣椒细胞质雄性不育与活性氧代谢的关系[J].西北植物学报,2005,25(4):799-802.ZHANG Z X,HOU X L.Relation between cytoplasmic male sterility and reactive oxygen species metabolism in pepper[J].Acta Botanica Boreali-Occidentalia Sinica,2005,25(4):799-802.(in Chinese with English abstract)
[31]张建奎,宗学凤,王俊义,等.温光敏核雄性不育小麦花药中保护酶活性的变化[J].麦类作物学报,2001,21(4):26-30.ZHANG J K,ZONG X F,WANG J Y,et al.Changes of activity of protective enzymes in anther of thermo-photo-sensitive genic male-sterile wheat[J].Journal of Triticeae Crops,2001,21(4):26-30.(in Chinese with English abstract)
[32]石晓艺,胡甘,蒙立颖,等.5种细胞质雄性不育小麦败育过程中活性氧代谢保护酶的响应[J].麦类作物学报,2014,34(12):1656-1660.SHI X Y,HU G,MENG L Y,et al.Activities of protective enzymes of reactive oxygen metabolism during aborting process of 5 kinds of cytoplasmic male wheat lines[J].Journal of Triticeae Crops,2014,34(12):1656-1660.(in Chinese with English abstract)
[33]刘莉.水稻细胞质雄性不育系和保持系表型观察及活性氧代谢研究[D].南京:南京师范大学,2016.LIU L.Studies on the phenotype observation and reactive oxygen metabolism of cytoplamsic male sterility and their maintainer lines in rice[D].Nanjing:Nanjing Normal University,2016.