霍乱毒素B亚单位对小鼠免疫细胞及细胞因子的影响
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摘要
目的探讨侧脑室注射霍乱毒素B亚单位(CTB)对小鼠免疫细胞及细胞因子的影响。方法 C57雌性小鼠侧脑室注射CTB,运用流式细胞术检测外周血免疫细胞亚群变化;流式微球捕获芯片技术(CBA)检测血清中细胞因子及趋化因子的变化。结果 CTB能够明显升高单核巨噬细胞、CD4~+T细胞及B细胞的比例;炎症因子γ干扰素(IFN-γ)、肿瘤坏死因子α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-9(IL-9)及趋化因子巨噬细胞炎性蛋白-1α(MIP-1α)、巨噬细胞炎性蛋白-1β(MIP-1β)、B淋巴细胞趋化因子(BLC)、γ干扰素诱导的单核因子(Mig)、调节活化正常T细胞表达与分泌的趋化因子(RANTES)、单核细胞趋化蛋白-1(MCP-1)也相应升高。结论侧脑室注射CTB能够明显改变小鼠免疫应答状态。
Objective To explore the effect of cholera toxin subunit B on the immune system of mice.Methods Intra-lateral ventricle(LV) of C57 female mice were injected with toxin subunit B.Immunocyte in peripheral blood were detected by flow cytometry; Cytokine and chemokines in serum were detected by flow cytometry with Cytometrin Bead Array(CBA).Results Data showed that CD3~+CD4~+T cells,B cells and mononuclear macrophage were higher than PBS control group. IFN-γ,TNF-α,IL-6, IL-9 inflammation cytokines and MIP-1α,MIP-1β,BLC,MIG,RANTES,MCP1 chemokine were higher than PBS control group.Conclusion Cholera toxin subunit B can obviously change the state of immune response in mice.
Objective To explore the effect of cholera toxin subunit B on the immune system of mice.Methods Intra-lateral ventricle(LV) of C57 female mice were injected with toxin subunit B.Immunocyte in peripheral blood were detected by flow cytometry; Cytokine and chemokines in serum were detected by flow cytometry with Cytometrin Bead Array(CBA).Results Data showed that CD3~+CD4~+T cells,B cells and mononuclear macrophage were higher than PBS control group. IFN-γ,TNF-α,IL-6, IL-9 inflammation cytokines and MIP-1α,MIP-1β,BLC,MIG,RANTES,MCP1 chemokine were higher than PBS control group.Conclusion Cholera toxin subunit B can obviously change the state of immune response in mice.
引文
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[13] Scully P, Macsharry J, O′Mahony D, et al. Bifidobacterium infantis suppression of Peyer′s patch MIP-1α and MIP-1β secretion during salmonella infection correlates with increased local CD4+CD25+ T cell numbers[J].Cell Immunol, 2013,281(2):134-40.
[14] Barbi J, Oghumu S, Rosas L E, et al. Lack of CXCR3 delays the development of hepatic inflammation but does not impair resistance to Leishmania donovani[J]. J Infect Dis, 2007,195(11):1713-7.
[15] Aldinucci D, Colombatti A. The inflammatory chemokine CCL5 and cancer progression[J]. Mediators Inflamm,2014,2014:292376.
[2] Baldauf K J, Royal J M, Hamorsky K T ,et al. Cholera toxin B: one subunit with many pharmaceutical applications[J]. Toxins,2015,7(3):974-96.
[3] Gloudemans A K, Plantinga M, Guilliams M,et al. The mucosal adjuvant cholera toxin B instructs non-mucosal dendritic cells to promote IgA production via retinoic acid and TGF-β[J].PLoS One,2013,8(3):e59822.
[4] Anosova N G, Chabot S, Shreedhar V,et al. Cholera toxin, E. coli heat-labile toxin, and non-toxic derivatives induce dendritic cell migration into the follicle-associated epithelium of Peyer′s patches[J]. Mucosal Immunol, 2008,1(1):59-67.
[5] Brunner R, Jensen-Jarolim E, Pali-Sch?ll I. The ABC of clinical and experimental adjuvants-a brief overview[J].Immunol Lett,2010,128(1):29-35.
[6] Wiedinger K, Romlein H, Bitsaktsis C. Cholera toxin B induced activation of murine macrophages exposed to a fixed bacterial immunogen[J]. Ther Adv Vaccines, 2015,3(5-6):155-63.
[7] Gubernatorova E O, Tumanov A V.Tumor necrosis factor and lymphotoxin in regulation of intestinal inflammation[J]. Biochemistry(Mosc),2016,81(11):1309-25.
[8] Su W L, Perng W C, Huang C H,et al. Association of reduced tumor necrosis factor alpha, gamma interferon, and interleukin-1beta(IL-1beta) but increased IL-10 expression with improved chest radiography in patients with pulmonary tuberculosis[J]. Clin Vaccine Immunol, 2010,17(2):223-31.
[9] Charrad R, Berra?es A, Hamdi B, et al.Anti-inflammatory activity of IL-37 in asthmatic children: correlation with inflammatory cytokines TNF-α, IL-β, IL-6 and IL-17A[J].Immunobiology,2016 ,221(2):182-7.
[10] 明婷婷,丁绍威,胡超杰,等. CD4+IL-9+T细胞在实验性动脉粥样硬化中的作用[J]. 安徽医科大学学报,2017,52(6):824-8.
[11] Chowdhury K, Kumar U, Das S, et al. Synovial IL-9 facilitates neutrophil survival, function and differentiation of Th17 cells in rheumatoid arthritis[J]. Arthritis Res Ther,2018, 20(1):18.
[12] Wang Q, Ren J, Morgan S,et al. Monocyte chemoattractant protein-1(MCP-1) regulates macrophage cytotoxicity in abdominal aortic aneurysm[J]. PLoS One,2014, 9(3):e92053.
[13] Scully P, Macsharry J, O′Mahony D, et al. Bifidobacterium infantis suppression of Peyer′s patch MIP-1α and MIP-1β secretion during salmonella infection correlates with increased local CD4+CD25+ T cell numbers[J].Cell Immunol, 2013,281(2):134-40.
[14] Barbi J, Oghumu S, Rosas L E, et al. Lack of CXCR3 delays the development of hepatic inflammation but does not impair resistance to Leishmania donovani[J]. J Infect Dis, 2007,195(11):1713-7.
[15] Aldinucci D, Colombatti A. The inflammatory chemokine CCL5 and cancer progression[J]. Mediators Inflamm,2014,2014:292376.