犊牛粪便中益生菌的分离与鉴定
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摘要
为了筛选出能够抑制犊牛腹泻的益生菌株,本研究采集了20份健康犊牛新鲜粪便,采用厌氧培养、形态学观察以及分子鉴定技术,初步分离得到3株革兰氏阳性杆菌(A1、A2和A3)和4株革兰氏阳性球菌(B1、B2、B3和B4),并对这些菌株进行分子鉴定。鉴定结果表明:A1和A2均为罗伊乳杆菌,A3为黏膜乳杆菌;B1为坚强肠球菌,B2为海氏肠球菌,B3和B4均为屎肠球菌。本研究为防治犊牛腹泻提供了更多的候选菌种。
In order to screen the inhibition of calf diarrhea probiotics, this study collected fresh faeces from 20 healthy calves by anaerobic cultivation. Using morphological observation and molecular identification technology, 7 probiotics strains were preliminarily isolated, and they were 3 strains of Gram-positive bacillus(A1, A2 and A3) and 4 strains of Gram-positive cocci(B1, B2, B3, and B4). The results showed that, A1 and A2 were Lactobacillus reuteri, A3 was Lactobacillus mucosae, B1 was Enterococcus durans, B2 was Enterococcus hirae, B3 and B4 were Enterococcus faecium based on 16 S rRNA gene. This study provides more candidates for the treatment of calf diarrhea.
In order to screen the inhibition of calf diarrhea probiotics, this study collected fresh faeces from 20 healthy calves by anaerobic cultivation. Using morphological observation and molecular identification technology, 7 probiotics strains were preliminarily isolated, and they were 3 strains of Gram-positive bacillus(A1, A2 and A3) and 4 strains of Gram-positive cocci(B1, B2, B3, and B4). The results showed that, A1 and A2 were Lactobacillus reuteri, A3 was Lactobacillus mucosae, B1 was Enterococcus durans, B2 was Enterococcus hirae, B3 and B4 were Enterococcus faecium based on 16 S rRNA gene. This study provides more candidates for the treatment of calf diarrhea.
引文
[1]温俊,孙冬岩,孙笑非.肠道菌群的重要性及微生态制剂对肠道的调节作用[J].饲料研究,2010,2:70-72.
[2]Gaggia F,Mattarelli P,Biavati B.Probiotics and prebiotics in animal feeding for safe food production[J].Int J Food Microbil,2010,141:15-28.
[3]Hill C,Guainer F,Reid G.et al.The international scientific Association for Probiotics and prebiotics concensus statement on the scope and appropriate use of the term probiotic[J].Nat Rev Gastroenterol Hepatol,2014,11:506-514.
[4]章文明,汪海峰,刘建新,等.乳酸杆菌益生作用机制的研究进展[J].动物营养学报,2012,24(3):389-396.
[5]杨汝德,陈琼,陈惠音,等.乳酸菌发酵制品研究的现状与发展[A].广州食品工业科技“益生菌、益生元与健康”研讨会论文集[C].2001.
[6]赵桂英,杨亮宇,段纲,等.断奶仔猪健康菌群的数量和分区[J].黑龙江畜牧兽医,2003,9:27-28.
[7]Coccorullo P,Strisciuglio C,Martinelli M,et al.Lactobacillus reuteri(DSM 17938)in infants with functional chronic constipation:A double-blind,randomized,placebo-controlled Study[J].J Pediatr,2010,157(4):598-602.
[8]Savino F,Pelle E,Palumeri E,et al.Lactobacillus reuteri(American Type Culture Collection Strain 55730)Versus Simethicone in the Treatment of Infantile Colic:A Prospective Randomized Study[J].Pediatrics,2007,119:124-130.
[9]王永,杨维仁,张桂国.饲料中添加屎肠球菌对断奶仔猪生长性能、肠道菌群和免疫功能的影响[J].动物营养学报,2013,25(5):1069-1076.
[10]Axelsson L,Chung T C,Dobrogosz W J,et al.Production of a broad spectrum antimicrobial substance by Lactobacillus reuteri[J].Microbial Ecol Health Dis,1989,2:131-136.
[11]Spinler J K,Taweechotipart M,Rognerud C L,et al.Human derived probiotic Lactobacillus reuteri demonstrate antimicrobial activities targeting diverse enteric bacterial pathogens[J].Anaerobe,2008,14(3):166-171.
[12]Pessi T,Sutas Y,Hurme M,et al.Interleukin-10generation in atopic children following oral Lactobacillus rhamnosus GG[J].Clin Exp Allergy,2000,30:1804-1808.
[13]姜绍通,罗水中,郑志,等.粘膜乳杆菌发酵乳清生产L-乳酸的研究[J].食品科学,2007,28(2):162-165.
[14]葛龙,李波.屎肠球菌在饲用微生态制剂中的研究与应用[J].饲料与畜牧,2013,6:57-59.
[2]Gaggia F,Mattarelli P,Biavati B.Probiotics and prebiotics in animal feeding for safe food production[J].Int J Food Microbil,2010,141:15-28.
[3]Hill C,Guainer F,Reid G.et al.The international scientific Association for Probiotics and prebiotics concensus statement on the scope and appropriate use of the term probiotic[J].Nat Rev Gastroenterol Hepatol,2014,11:506-514.
[4]章文明,汪海峰,刘建新,等.乳酸杆菌益生作用机制的研究进展[J].动物营养学报,2012,24(3):389-396.
[5]杨汝德,陈琼,陈惠音,等.乳酸菌发酵制品研究的现状与发展[A].广州食品工业科技“益生菌、益生元与健康”研讨会论文集[C].2001.
[6]赵桂英,杨亮宇,段纲,等.断奶仔猪健康菌群的数量和分区[J].黑龙江畜牧兽医,2003,9:27-28.
[7]Coccorullo P,Strisciuglio C,Martinelli M,et al.Lactobacillus reuteri(DSM 17938)in infants with functional chronic constipation:A double-blind,randomized,placebo-controlled Study[J].J Pediatr,2010,157(4):598-602.
[8]Savino F,Pelle E,Palumeri E,et al.Lactobacillus reuteri(American Type Culture Collection Strain 55730)Versus Simethicone in the Treatment of Infantile Colic:A Prospective Randomized Study[J].Pediatrics,2007,119:124-130.
[9]王永,杨维仁,张桂国.饲料中添加屎肠球菌对断奶仔猪生长性能、肠道菌群和免疫功能的影响[J].动物营养学报,2013,25(5):1069-1076.
[10]Axelsson L,Chung T C,Dobrogosz W J,et al.Production of a broad spectrum antimicrobial substance by Lactobacillus reuteri[J].Microbial Ecol Health Dis,1989,2:131-136.
[11]Spinler J K,Taweechotipart M,Rognerud C L,et al.Human derived probiotic Lactobacillus reuteri demonstrate antimicrobial activities targeting diverse enteric bacterial pathogens[J].Anaerobe,2008,14(3):166-171.
[12]Pessi T,Sutas Y,Hurme M,et al.Interleukin-10generation in atopic children following oral Lactobacillus rhamnosus GG[J].Clin Exp Allergy,2000,30:1804-1808.
[13]姜绍通,罗水中,郑志,等.粘膜乳杆菌发酵乳清生产L-乳酸的研究[J].食品科学,2007,28(2):162-165.
[14]葛龙,李波.屎肠球菌在饲用微生态制剂中的研究与应用[J].饲料与畜牧,2013,6:57-59.
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