HPLC法测定棘豆止咳散中甘草苷的含量
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摘要
目的:建立棘豆止咳散中甘草苷的含量测定方法。方法:采用XTerra MSC18(4.6 mm×250 mm, 5.0μm)色谱柱,乙腈(A)-0.05%磷酸水(B)为流动相进行梯度洗脱,流速1.0 mL/min,检测波长237 nm,柱温40℃。结果:HPLC法测定甘草苷线性关系良好(r~2=0.9993),精密度、稳定性、重复性的RSD值均小于3.0%,加样回收率为90.19%～97.87%。结论:所建立的方法简便,可靠,重复性好,可用于棘豆止咳散的质量控制。
Objective To establish a method for content determination of Liquiritin in Jidouzhike powder. Methods X Terra? MSC18(4.6 mm×250 mm,5.0 μm)was used as chromatographic column, the mobile phase was acetonitrile(A)-0.05% phosphate water(B) with gradient elution at a flow rate of 1.0 mL/min, the detection wavelength was 237 nm, and column temperature was 40℃. Results Liquiritin showed a good linearity in the range of the test concentrations(r~2=0.9993), the RSD values of precision, stability and repeatability were less than 3.0%, and the recovery rate was 90.19% to 97.87%. Conclusion The method established in the experiment is highly sensitive, stable and reliable, and can be used for quality control of Jidouzhike powder.
Objective To establish a method for content determination of Liquiritin in Jidouzhike powder. Methods X Terra? MSC18(4.6 mm×250 mm,5.0 μm)was used as chromatographic column, the mobile phase was acetonitrile(A)-0.05% phosphate water(B) with gradient elution at a flow rate of 1.0 mL/min, the detection wavelength was 237 nm, and column temperature was 40℃. Results Liquiritin showed a good linearity in the range of the test concentrations(r~2=0.9993), the RSD values of precision, stability and repeatability were less than 3.0%, and the recovery rate was 90.19% to 97.87%. Conclusion The method established in the experiment is highly sensitive, stable and reliable, and can be used for quality control of Jidouzhike powder.
引文
[1]扎拉嘎白乙拉.蒙药棘豆止咳散[P].中国专利:CN105596695A,2016-05-25.
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[13]张明,邓毅.甘草及其有效成分的药效学研究进展[J].西部中医药,2015,28(4):156-159.
[14]李静.甘草的临床应用进展[J].中国保健营养,2012,8(4):598-599.
[2]国家药典委员会.中华人民共和国药典(一部) [S].北京:中国医药科技出版社,2015.
[3]李旋珠,陆思选.甘草止咳有奇效[J].云南中医杂志,1992(3):21.
[4]袁立友.甘草在止咳化痰药中的应用[J].天津药学,2001,13(1):28-29.
[5]李薇,宋新波,张丽娟.甘草中化学成分研究进展 [J].辽宁中医药大学学报,2012,14(7):40-43.
[6]惠寿年,董阿玲.国内对甘草化学成分的研究进展[J].中草药,1999,30(4):313-315.
[7]朱一亮,谢强敏,陈季强.甘草黄酮对辣椒素诱导豚鼠咳嗽反射的抑制作用[J].中草药,2006,37(7):1048-1051.
[8]马玲.甘草黄酮及皂苷类成分谱效关系研究[D].银川:宁夏医科大学,2015.
[9]布和巴特尔.传统蒙药与方剂[M].赤峰:内蒙古科学技术出版社,2013:95.
[10]李萍,季辉,徐国钧,等.贝母类中药的镇咳祛痰作用研究[J].中国药科大学学报,1993,24(6) :360 - 362.
[11]孙涛,徐颖,高飞,等.基于P物质水平的川贝母止嗽颗粒止咳作用及机制研究[J].中药药理与床,2014,30(3):127 -130.
[12]周宜,丁红,阎博华,等.不同基源川贝母镇咳、祛痰功效差异性实验研究[J ].中国临床药药理学与治疗学,2010,15(6):612-616.
[13]张明,邓毅.甘草及其有效成分的药效学研究进展[J].西部中医药,2015,28(4):156-159.
[14]李静.甘草的临床应用进展[J].中国保健营养,2012,8(4):598-599.