双波长HPLC法测定防风通圣丸中6种成分的含量
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摘要
目的建立双波长HPLC法同时测定防风通圣丸中6种成分的含量。方法日本岛津C18色谱柱(250mm×4.6mm,5μm);以乙腈-0.5mL·L-1磷酸水溶液为流动相,梯度洗脱;流速:1.0mL·min-1;柱温:25℃;栀子苷、芍药苷、连翘苷、甘草苷和甘草酸铵的检测波长均为237nm,黄芩苷的检测波长为280nm。结果栀子苷、芍药苷、甘草苷、黄芩苷、连翘苷和甘草酸铵分别在0.041～0.414,0.048～0.487,0.062～0.622,0.057～0.568,0.042～0.417和0.080～0.796μg范围内线性关系良好;其平均回收率(n=5)分别为99.6%,98.2%,99.7%,100.0%,101.3%和107.9%。结论该方法准确、专属性好,可用于防风通圣丸的质量控制。
Objective To establish a double wavelength HPLC method for the simultaneous determination of 6components in Fangfengtongsheng Pills.Methods A double wavelength HPLC was developed,and the analysis was performed on a SHIMADZU C18column(250mm×4.6mm,5μm)with gradient elution using acetonitrile-0.5mL·L-1 phosphoric acid as mobile phase in a flow rate of 1.0mL·min-1.Geniposide,paeoniflorin,forsyside,liquiritin and ammonium glycyrrhizinate were detected at 237 nm and baicalin was detected at 280 nm.The column temperature was 25℃.Results The linear ranges of geniposide,paeoniflorin,liquiritin,baicalin,forsyside and ammonium glycyrrhizinate were 0.041-0.414,0.048-0.487,0.062-0.622,0.057-0.568,0.042-0.417and0.080-0.796μg,respectively.The average recoveries(n=5)were 99.6%,98.2%,99.7%,100.0%,101.3%and 107.9%,respectively.Conclusion The method is accurate,reproducible and can be used for the quality control of Fangfengtongsheng Pills
Objective To establish a double wavelength HPLC method for the simultaneous determination of 6components in Fangfengtongsheng Pills.Methods A double wavelength HPLC was developed,and the analysis was performed on a SHIMADZU C18column(250mm×4.6mm,5μm)with gradient elution using acetonitrile-0.5mL·L-1 phosphoric acid as mobile phase in a flow rate of 1.0mL·min-1.Geniposide,paeoniflorin,forsyside,liquiritin and ammonium glycyrrhizinate were detected at 237 nm and baicalin was detected at 280 nm.The column temperature was 25℃.Results The linear ranges of geniposide,paeoniflorin,liquiritin,baicalin,forsyside and ammonium glycyrrhizinate were 0.041-0.414,0.048-0.487,0.062-0.622,0.057-0.568,0.042-0.417and0.080-0.796μg,respectively.The average recoveries(n=5)were 99.6%,98.2%,99.7%,100.0%,101.3%and 107.9%,respectively.Conclusion The method is accurate,reproducible and can be used for the quality control of Fangfengtongsheng Pills
引文
[1]国家药典委员会.中国药典:2012年版:第一增补本[S].北京:中国医药科技出版社,2012:176-177.
[2]国家药典委员会.中国药典:2015年版:一部[S].北京:中国医药科技出版社,2015:889.
[3]黄敬群,杨娟,李红.高效液相色谱法测定双黄连口服液中绿原酸、黄芩苷、连翘苷、汉黄芩苷的含量[J].中国中医药信息杂志,2008,15(5):53-55.
[4]侯志坚,师永清.双波长同时测定防风通圣丸中栀子苷和黄芩苷的含量[J].中国实验方剂学杂志,2011,17(24):80-82.
[5]王敏,杜守颖,杨冰,等.HPLC法同时测定益气复脉方中3种活性成分的含量[J].西北药学杂志,2017,32(3):260-263.
[6]宋丽丽,曹颖,李晓霞,等.应用HPLC对复方一清颗粒中黄芩苷含量测定方法的改进[J].实验室科学,2015,18(2):10-13.
[7]张囡,钱宇坤,李维超.病毒合剂(Ⅱ)中黄芩苷、绿原酸和橙皮苷的含量测定[J].天津药学,2015,27(3):13-15.
[8]孙晓佳,纪宏宇,兰恭赞,等.HPLC法测定乳癖消颗粒中连翘苷的含量[J].中国药师,2016,19(1):184-186.
[9]翟媛媛,师永清.HPLC法同时测定清心明目上清片中栀子苷、连翘苷、黄芩苷和盐酸小檗碱的含量[J].西北民族大学学报:自然科学版,2016,37(1):13-17.
[10]张丹丹,冯程,顾媛媛,等,UPLC同时测定双黄连粉针剂中黄芩苷、绿原酸、连翘苷成分的含量[J].中医药信息,2017,34(2):39-41.
[11]郝乘仪,郭淑英,冯波,等.高效液相色谱法同时测定牛黄上清丸中6个成分的含量[J].中国药学杂志,2014,49(9):769-772.
[12]尹雪,孙萍,温学森.白芍中芍药苷含量影响因素研究进展[J].辽宁中医药大学学报,2016,18(2):78-80.
[13]张艳.HPLC法同时测定泻肝安神丸中龙胆苦苷、栀子苷、黄芩苷的含量[J].中国药师,2017,20(6):1125-1127.
[14]杨必浩,周应硕.高效液相色谱法同时测定龙胆泻肝片中龙胆苦苷、栀子苷和黄芩苷的含量[J].系统医学,2017,2(8):127-131.
[15]高旭东,师永清,陈士恩,等.双波长HPLC测定栀子金花丸中栀子苷、黄芩苷、盐酸小檗碱和大黄素的含量[J].国际药学研究杂志,2015,42(3):409-412.
[16]陈国辉.高效液相色谱法测定双黄连口服液中连翘苷含量[J].海峡药学,2015,27(10):70-71.
[17]田园,王海亮,解笑瑜,等.HPLC法测定和血明目片中黄芩苷的含量[J].西北药学杂志,2017,29(4):361-363.
[18]赵咏梅,王雷.连翘苷的提取及其稳定性研究[J].陕西农业科学,2016,62(5):21-23.
[19]宋佳,段树卿,李云霞.HPLC法同时测定心神宁片中栀子苷和甘草苷的含量[J].中国药房,2015,26(27):3862-3864.
[20]张丽萍.用高效液相色谱法测定安坤颗粒中栀子苷和芍药苷的含量[J].药学实践杂志,2014,32(2):121-123.
[21]臧文怡,毛春芹,苏晓楠,等.HPLC同时测定益经颗粒中芍药苷、阿魏酸、毛蕊花糖苷和斯皮诺素的含量[J].中国实验方剂学杂志,2016,22(5):60-63.
[22]王书航,金向群,刘玉梅.高效液相色谱法测定藿香清胃软胶囊中栀子苷和甘草苷的含量[J].中国临床药理学杂志,2015,31(20):2049-2051.
[23]赵亮,王新霞,吕磊,等.HPLC法测定清肝散结颗粒中黄芩苷、汉黄芩苷、野黄芩苷、黄芩素及汉黄芩素[J].中成药,2014,36(2):313-317.
[2]国家药典委员会.中国药典:2015年版:一部[S].北京:中国医药科技出版社,2015:889.
[3]黄敬群,杨娟,李红.高效液相色谱法测定双黄连口服液中绿原酸、黄芩苷、连翘苷、汉黄芩苷的含量[J].中国中医药信息杂志,2008,15(5):53-55.
[4]侯志坚,师永清.双波长同时测定防风通圣丸中栀子苷和黄芩苷的含量[J].中国实验方剂学杂志,2011,17(24):80-82.
[5]王敏,杜守颖,杨冰,等.HPLC法同时测定益气复脉方中3种活性成分的含量[J].西北药学杂志,2017,32(3):260-263.
[6]宋丽丽,曹颖,李晓霞,等.应用HPLC对复方一清颗粒中黄芩苷含量测定方法的改进[J].实验室科学,2015,18(2):10-13.
[7]张囡,钱宇坤,李维超.病毒合剂(Ⅱ)中黄芩苷、绿原酸和橙皮苷的含量测定[J].天津药学,2015,27(3):13-15.
[8]孙晓佳,纪宏宇,兰恭赞,等.HPLC法测定乳癖消颗粒中连翘苷的含量[J].中国药师,2016,19(1):184-186.
[9]翟媛媛,师永清.HPLC法同时测定清心明目上清片中栀子苷、连翘苷、黄芩苷和盐酸小檗碱的含量[J].西北民族大学学报:自然科学版,2016,37(1):13-17.
[10]张丹丹,冯程,顾媛媛,等,UPLC同时测定双黄连粉针剂中黄芩苷、绿原酸、连翘苷成分的含量[J].中医药信息,2017,34(2):39-41.
[11]郝乘仪,郭淑英,冯波,等.高效液相色谱法同时测定牛黄上清丸中6个成分的含量[J].中国药学杂志,2014,49(9):769-772.
[12]尹雪,孙萍,温学森.白芍中芍药苷含量影响因素研究进展[J].辽宁中医药大学学报,2016,18(2):78-80.
[13]张艳.HPLC法同时测定泻肝安神丸中龙胆苦苷、栀子苷、黄芩苷的含量[J].中国药师,2017,20(6):1125-1127.
[14]杨必浩,周应硕.高效液相色谱法同时测定龙胆泻肝片中龙胆苦苷、栀子苷和黄芩苷的含量[J].系统医学,2017,2(8):127-131.
[15]高旭东,师永清,陈士恩,等.双波长HPLC测定栀子金花丸中栀子苷、黄芩苷、盐酸小檗碱和大黄素的含量[J].国际药学研究杂志,2015,42(3):409-412.
[16]陈国辉.高效液相色谱法测定双黄连口服液中连翘苷含量[J].海峡药学,2015,27(10):70-71.
[17]田园,王海亮,解笑瑜,等.HPLC法测定和血明目片中黄芩苷的含量[J].西北药学杂志,2017,29(4):361-363.
[18]赵咏梅,王雷.连翘苷的提取及其稳定性研究[J].陕西农业科学,2016,62(5):21-23.
[19]宋佳,段树卿,李云霞.HPLC法同时测定心神宁片中栀子苷和甘草苷的含量[J].中国药房,2015,26(27):3862-3864.
[20]张丽萍.用高效液相色谱法测定安坤颗粒中栀子苷和芍药苷的含量[J].药学实践杂志,2014,32(2):121-123.
[21]臧文怡,毛春芹,苏晓楠,等.HPLC同时测定益经颗粒中芍药苷、阿魏酸、毛蕊花糖苷和斯皮诺素的含量[J].中国实验方剂学杂志,2016,22(5):60-63.
[22]王书航,金向群,刘玉梅.高效液相色谱法测定藿香清胃软胶囊中栀子苷和甘草苷的含量[J].中国临床药理学杂志,2015,31(20):2049-2051.
[23]赵亮,王新霞,吕磊,等.HPLC法测定清肝散结颗粒中黄芩苷、汉黄芩苷、野黄芩苷、黄芩素及汉黄芩素[J].中成药,2014,36(2):313-317.