姜黄素及姜黄提取物对血栓模型大鼠凝血功能的影响
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摘要
目的初步探讨姜黄素及姜黄提取物对血栓模型大鼠凝血功能的影响,为进一步研究其作用机制做前期准备。方法分别用姜黄素及姜黄提取物灌胃14d后,采用物理方法建立大鼠血栓模型,获取各组大鼠血浆,全自动凝血仪检测活化部分凝血活酶时间(APTT)、纤维蛋白原(FIB)、凝血酶时间(TT)及抗凝血酶3(AT3)。结果血栓模型鼠血栓形成后,血浆APTT显著延长(P=0.011);姜黄素低、高剂量灌胃处理的血栓模型鼠血浆APTT缩短(P=0.02,P<0.001),而姜黄提取物处理的血栓模型鼠血浆APTT进一步延长(P<0.001,P=0.019);血栓模型鼠血栓形成后血浆FIB显著升高(P<0.001),姜黄素及姜黄提取物低、高剂量对血浆FIB没有显著影响;姜黄素灌胃缩短TT时间(P=0.03,P=0.001),而姜黄提取物没有此作用;姜黄素有降低AT3的作用,而姜黄提取物有升高AT3的作用。结论姜黄素没有抗凝作用,而姜黄提取物则表现出较强的抗凝作用,提示姜黄提取物的活血化瘀机制与抗凝作用有关,而姜黄素的作用机制有待进一步探讨。
Objective To study the effects of curcumin and turmeric extract on coagulation function in rats with thrombotic model,and to prepare for further study of its mechanism.Methods After 14 days of intragastric administration of curcumin and turmeric extract,the thrombosis model of rats was established by physical method.The plasma of rats in each group was obtained.The activated partial thromboplastin time(APTT),fibrinogen(FIB),thrombin time(TT)and antithrombin 3(AT3)were detected by automatic coagulation analyzer.Results The plasma APTT was significantly prolonged after thrombosis in thrombotic model rats(P=0.011),and the plasma APTT in thrombotic model rats treated with curcumin at low and high doses by gavage was shortened(P=0.02,P<0.001),while the plasma APTT of thrombotic model rats treated with turmeric extract was further prolonged(P<0.001,P=0.019),and the plasma FIB increased significantly after thrombosis inthrombotic model rats(P<0.001).Curcumin and turmeric extract at low and high doses had no significant effect on plasma FIB level,curcumin gavage decreased TT(P=0.03,P=0.001),but turmeric extract did not have this function.Curcumin tended to decrease AT3,and turmeric extract had an effect of increasing AT3.Conclusion Curcumin has no anticoagulant effect,but turmeric extract shows strong anticoagulant effect,suggesting that the mechanism of activating blood circulation and removing blood stasis of turmeric extract is related to anticoagulant effect,and the mechanism of curcumin action needs to be further explored.
Objective To study the effects of curcumin and turmeric extract on coagulation function in rats with thrombotic model,and to prepare for further study of its mechanism.Methods After 14 days of intragastric administration of curcumin and turmeric extract,the thrombosis model of rats was established by physical method.The plasma of rats in each group was obtained.The activated partial thromboplastin time(APTT),fibrinogen(FIB),thrombin time(TT)and antithrombin 3(AT3)were detected by automatic coagulation analyzer.Results The plasma APTT was significantly prolonged after thrombosis in thrombotic model rats(P=0.011),and the plasma APTT in thrombotic model rats treated with curcumin at low and high doses by gavage was shortened(P=0.02,P<0.001),while the plasma APTT of thrombotic model rats treated with turmeric extract was further prolonged(P<0.001,P=0.019),and the plasma FIB increased significantly after thrombosis inthrombotic model rats(P<0.001).Curcumin and turmeric extract at low and high doses had no significant effect on plasma FIB level,curcumin gavage decreased TT(P=0.03,P=0.001),but turmeric extract did not have this function.Curcumin tended to decrease AT3,and turmeric extract had an effect of increasing AT3.Conclusion Curcumin has no anticoagulant effect,but turmeric extract shows strong anticoagulant effect,suggesting that the mechanism of activating blood circulation and removing blood stasis of turmeric extract is related to anticoagulant effect,and the mechanism of curcumin action needs to be further explored.
引文
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[13]周丽娟,赵军宁,徐海波,等.不同商品规格,等级姜黄提取物抗氧化作用比较研究[J].中药药理与临床,2016,32(1):110-112.
[2] PENN M S,IGWE C.Role of inflammation in modulating thrombotic-fibrinolytic balance in venous thrombosis[J].Circ Res,2016,119(12):1256-1257.
[3] KIM K H,BARAZIA A,CHO J.Real-time imaging of heterotypic platelet-neutrophil interactions on the activated endothelium during vascular inflammation and thrombus formation in live mice[J].J Vis Exp,2013,2(74):e50329.
[4]IBA T,LEVY J H.Inflammation and thrombosis:roles of neutrophils,plateletsandendothelial cells and their interactions in thrombus formation during sepsis[J].J Thromb Haemost,2018,16(2):231-241.
[5] KOCAADAM B,SANLIER N.Curcumin,an active component of turmeric(Curcuma longa),and its effects on health[J].Crit Rev Food Sci Nutr,2017,57(13):2889-2895.
[6] KARIMIAN M S,PIRRO M,MAJEED M A.Curcumin as a natural regulator of monocyte chemoattractant protein-1[J].Cytokine Growth Factor Rev,2017,33:55-63.
[7] SHI X,ZHENG Z,LI J,et al.Curcumin inhibits Aβ-induced microglial inflammatory responses in vitro:Involvement of ERK1/2and p38signaling pathways[J].Neurosci Lett,2015,594:105-110.
[8] WOJCIK M,KRAWCZYK M,WOJCIK P,et al.Molecular mechanisms underlying curcumin-mediated therapeutic effects in type 2diabetes and cancer[J].Oxid Med Cell Longev,2018:9698258.
[9] AHMAD I,SHARMA S,GUPTA N,et al.Antithrombotic potential of esculin 7,3′,4′,5′,6′-O-pentasulfate(EPS)for its role in thrombus reduction using rat thrombosis model[J].Int J Biol Macromol,2018,119:360-368.
[10]HASAN M,LATIFI S,KAHN C J,et al.The positive role of curcumin-loaded salmon nanoliposomes on the culture of primary cortical neurons[J].Mar Drugs,2018,16(7):218.
[11]肖志勇.姜黄提取物对小鼠肝纤维化的影响[J].生物技术通讯,2018,29(4):516-520.
[12]周丽娟,赵军宁,徐海波,等.不同来源姜黄提取物的抗肝损伤药效学研究[J].中药药理与临床,2015,31(2):55-57.
[13]周丽娟,赵军宁,徐海波,等.不同商品规格,等级姜黄提取物抗氧化作用比较研究[J].中药药理与临床,2016,32(1):110-112.