甜瓜侧枝相关性状的QTL定位
|
摘要
以甜瓜长侧枝自交系‘TopMark’和短侧枝自交系‘PI353814’为亲本,构建了F_2群体和F_(2:3)家系。利用92个F_2单株构建了一张包含12个连锁群,353个SSR标记的遗传连锁图谱,覆盖基因组长度为1 565.88 cM,连锁群长度在86.98～186.68 cM,标记间的平均距离为4.44 cM。利用该连锁图谱结合F_(2:3)家系表型鉴定数据,对甜瓜侧枝相关性状进行定位,共检测到6个QTL,分布在连锁群LGⅠ、LGⅢ和LGⅦ上,LOD值介于2.5067～12.5524之间,可解释9.9242%～48.2348%的表型变异率。其中侧枝总长主效QTLlbtl7.1和侧枝均长主效QTLlbal7.1均位于连锁群LGVⅡ的SSR标记CmSSR17145和CmSSR17293之间,贡献率分别为38.5923%和48.2348%。进一步利用186个F_(2:3)家系和新开发标记对主效QTL进行了验证,发现侧枝总长主效QTL lbtl7.1定位在SSR标记CmSSR17251和CmSSR17253之间,与两标记的遗传距离分别为2.5和0.5 cM,可解释41.2742%的表型变异,侧枝均长主效QTL lbal7.1被定位在SSR标记CmSSR17238和CmSSR17251之间,距离两标记分别为1.5和0.5cM,可解释55.1739%的表型变异。
To map lateral branch associated QTL in melon,a long lateral branch inbred line‘Top Mark'and a short lateral branch inbred line‘PI 353814'was crossed to generate a F_2 population and F_(2:3) line in this study. Using a mapping population containing 92 F_2 plants and the SSR markers developed from melon genome,a genetic map containing 353 SSR markers was constructed. The total genetic map covered 1 565.88 cM with an average distance between interval markers of 4.44 cM. The genetic length of these linkage groups ranged from 86.98 cM to 186.68 cM. QTL analysis was carried out by combining the phenotypes of lateral branch related traits in F_(2:3) lines and the genotyping data,and 6 QTLs for these traits were detected on LGⅠ,LGⅢ and LGⅦ,explaining 9.9242% ～ 48.2348% of total phenotypic variation.Among them,two major QTL lbtl7.1 and lbal7.1 were mapped on chromosome LGVⅡ between markers CmSSR17145 and CmSSR17293,explaining 38.5923% and 48.2348% of the phenotypic variation,respectively. In order to validate these major QTLs,new SSR markers were developed in the candidate region,and an extended population of 186 F_(2:3) lines were used for QTL mapping. The results showed that the major QTL lbtl7.1 was further mapped between CmSSR17251 and CmSSR17253 markers with a genetic distance of 2.5 cM and 0.5 cM,explaining 41.2742% of the phenotypic variation. Another major QTL lbal7.1 was further mapped between CmSSR17238 and CmSSR17251,with a genetic distance of 1.5 cM and 0.5 cM,respectively,explaining 55.1739% phenotypic variation.
To map lateral branch associated QTL in melon,a long lateral branch inbred line‘Top Mark'and a short lateral branch inbred line‘PI 353814'was crossed to generate a F_2 population and F_(2:3) line in this study. Using a mapping population containing 92 F_2 plants and the SSR markers developed from melon genome,a genetic map containing 353 SSR markers was constructed. The total genetic map covered 1 565.88 cM with an average distance between interval markers of 4.44 cM. The genetic length of these linkage groups ranged from 86.98 cM to 186.68 cM. QTL analysis was carried out by combining the phenotypes of lateral branch related traits in F_(2:3) lines and the genotyping data,and 6 QTLs for these traits were detected on LGⅠ,LGⅢ and LGⅦ,explaining 9.9242% ～ 48.2348% of total phenotypic variation.Among them,two major QTL lbtl7.1 and lbal7.1 were mapped on chromosome LGVⅡ between markers CmSSR17145 and CmSSR17293,explaining 38.5923% and 48.2348% of the phenotypic variation,respectively. In order to validate these major QTLs,new SSR markers were developed in the candidate region,and an extended population of 186 F_(2:3) lines were used for QTL mapping. The results showed that the major QTL lbtl7.1 was further mapped between CmSSR17251 and CmSSR17253 markers with a genetic distance of 2.5 cM and 0.5 cM,explaining 41.2742% of the phenotypic variation. Another major QTL lbal7.1 was further mapped between CmSSR17238 and CmSSR17251,with a genetic distance of 1.5 cM and 0.5 cM,respectively,explaining 55.1739% phenotypic variation.
引文
Beavis W D,Smith O S,Grant D,Fincher R.1994.Identification of quantitative trait loci using a small Sample of topcrossed and F4 progeny from maize.Crop Science,34(4):882-896.
Cho Y G,Eun M Y,Kim Y K,Chung T Y,Chae Y A.1994.The semidwarf gene,sd-1,of rice(Oryza sativa L.).I.Linkage with the esterase locus,Estl-2.Theoretical&Applied Genetics,89(1):49-53.
Ding An-ming,Cui Fa,LI Jun,Zhao Chun-hua,Wang Xiu-qin,Wang Hong-gang.2011.QTL analysis on grain yield per plant and plant height in wheat.Scientia Agricultura Sinica,44(14):2857-2867.
Fazio G,Chung S M,Staub J E.2003.Comparative analysis of response to phenotypic and marker-assisted selection for multiple lateral branching in cucumber(Cucumis sativus L.).Theoretical&Applied Genetics,107(5):875-883.
Fukino N,Ohara T,Sugiyama M,Kubo N,Hirai M,Sakata Y,Matsumoto S.2012.Mapping of a gene that confers short lateral branching(slb)in melon(Cucumis melo L.).Euphytica,187(1):133-143.
Jiang Su,Cai Run,Pan Jun-song,He Huan-le.2009.Anatomy observation and morphogenetic causation of non-lateral branch cucumber line S61.Acta Horticulturae Sinica,36(7):983-988.(in Chinese)蒋苏,蔡润,潘俊松,何欢乐.2009.黄瓜无侧枝品系S61的形态解剖学观察及其无侧枝成因分析.园艺学报,36(7):983-988.
Jiang Su,Yuan Xiao-jun,Pan Jun-song,He Huan-le,Cai Run.2008.Quantitative trait locus analysis of lateral branch-related traits in cucumber(Cucumis sativus L.)using recombinant inbred lines.Science in China Series C:Life Sciences,51(9):833-841.
Li S S,Jia J Z,Wei X Y,Zhang X C,Li L Z,Chen H M,Fan Y D,Sun H Y,Zhao X H,Lei T D,Xu Y F,Jiang F S,Wang H G,Li L H.2007.A intervarietal genetic map and QTL analysis for yield traits in wheat.Molecular Breeding,20:167-178.
Li Xiao-zun,Pan Jun-song,Wang Gang,Tian Li-bo,Si Long-ting,Wu Ai-zhong,Cai Run.2005.Localization of genes for lateral branch and female sex expression and construction of a molecular linkage map in cucumber(Cucumis sativus L.)with RAPD markers.Progress in Natural Science:Materials International,15(2):143-148.
Lu Fang.2009.Construction of permanent genetic map with SSR makers and mapping agronomic traits in hami melon[Cucumis melo L.ssp.melo.convar ameri(Pang.)Greb.][M.D.Dissertation].Harbin:Northeast Agricultural University.(in Chinese)陆芳.2009.哈密瓜SSR永久遗传图谱构建及重要农艺性状定位分析[硕士论文].哈尔滨:东北农业大学.
Ma Hai-cai,Ma Xiong,Liu Jian-li,Gong Hong-dong.2010.Construction of a molecular genetic map for melon with SSR markers.Journal of Fujian Agriculture and Forestry University(Natural Science Edition),39(1):47-52.(in Chinese)马海财,马雄,柳剑丽,巩红冬.2010.利用SSR分子标记构建甜瓜遗传图谱.福建农林大学学报(自然版),39(1):47-52.
Ma H,Zhang S,Ji L,Zhu H,Yang S,Fang X.2006.Fine mapping and in silico,isolation of the EUI1,gene controlling upper internode elongation in rice.Plant Molecular Biology,60(1):87-94.
Ohara T,Wako T,Kojima A,Yoshida,T.Ishiuchi D.2002.Breeding of suppressed-branching melon line‘Melon Chukanbohon Nou 4’(‘Melon parental line 4’)and its characteristics.Acta Horticulturae,588(588):227-231.
Qi Zheng-yu,Li Jun-xing,Zou Xiao-xia,Cao Li-wen,Rao Lin-li,Yu Jin-long,Chen Li-ping.2015.Genetic analysis of plant architecture traits in melon(Cucumis melo L.).Journal of Agricultural Biotechnology,23(3):302-310.(in Chinese)齐振宇,李俊星,邹晓霞,曹丽雯,饶琳莉,郁金龙,陈利萍.2015.甜瓜株型性状的遗传分析.农业生物技术学报,23(3):302-310.
Rebetzke G J,Richards R A.2000.Gibberellic acid-sensitive dwarfing genes reduce plant height to increase kernel number and grain yield of wheat.Australian Journal of Agricultural Research,51(2):235-246.
Ren Guo-liang,Yang Xu-qin,He Huan-le,Cai Run,Pan Jun-song.2013.Genetic mapping of the non-lateral branch gene(nlb)in cucumber.Acta Horticulturae Sinica,40(7):1375-1381.(in Chinese)任国良,杨绪勤,何欢乐,蔡润,潘俊松.2013.黄瓜无侧枝基因nlb的初步定位.园艺学报,40(7):1375-1381.
Serquen F C,Bacher J,Staub J E.1997.Mapping and QTL analysis of horticultural traits in a narrow cross in cucumber(Cucumis sativas L.)using random amplified polymorphic DNA makers.Molecular Breeding,3(4):257-268.
Wang G,Pan J S,Li X,Huanle H E.2005.Construction of a cucumber genetic linkage map with srap markers and location of the genes for lateral branch traits.Science in China,48(3):21-28.
Wei L,Xu J,Li X,Qian Q,Zhu L.2006.Genetic analysis and mapping of the dominant dwarfing gene D-53 in rice.Journal of Integrative Plant Biology,48(4):447-452.
Xing A,Gao Y,Ye L,Zhang W,Cai L,Ching A,Llaca V,Johnson B,Liu L,Yang X,Kang D,Yan J,Li J.2015.A rare SNP mutation in Brachytic2 moderately reduces plant height and increases yield potential in maize.Journal of Experimental Botany,66(13):3791.
Yang L,Koo D,Li D,Zhang T,Jiang J,Luan F,Renner S S,Henaff E,Sanseverino W,Garcia-Mas J,Casacuberta J,Senalik D A,Simon P W,Chen J,Weng Y.2014.Next-generation sequencing,FISH mapping and synteny-based modeling reveal mechanisms of decreasing dysploidy in Cucumis.The Plant Journal,77:16-30.
Zalapa J,Staub J,McCreight J.2008.Variance component analysis of plant architectural traits and fruit yield in melon.Euphytica,162(1):129-143.
Zhang Wei-wei,Pan Jun-song,Jiang Su,He Huan-le,Yu Ping-gao,Cai Run.2017.Genetic mapping of QTL associated with lateral branch-related traits using recombinant inbred lines population in cucumber(Cucumis sativus L.).Jiangsu J of Agr Sci,33(1):174-180.(in Chinese)张微微,潘俊松,蒋苏,何欢乐,俞平高,蔡润.2017.黄瓜RIL群体侧枝相关性状QTL定位.江苏农业学报,33(1):174-180.
Zhang Xue-jiao,Gao Peng,Luan Fei-shi.2013.The QTL analysis of traits related fruits in melon(Cucumis melo L.).China Vegetables,1(18):35-41.(in Chinese)张雪娇,高鹏,栾非时.2013.甜瓜果实相关性状QTL分析.中国蔬菜,1(18):35-41.
Zhang Y,Li Y X,Wang Y,Liu Z Z,Liu C,Peng B,Tan W W,Wang D,Shi Y S,Sun B C,Song Y C,Wang T Y,Li Y.2010.Stability of QTL across environments and QTL-by-Environment interactions for plant and ear height in maize.Agric Sci China,9(10):1400-1412.
Zhao Guang-wei,Xiong Jiang-ling,He Yu-hua,Wang Ping-yong,Kong Wei-hu,Zhang Jian,Xu Zhi-hong,Xu Yong-yang.2017.Research progresses on architecture related traits of melon.China Cucurbits and Vegetables,30(12):1-3,8.(in Chinese)赵光伟,熊姜玲,贺玉花,王平勇,孔维虎,张健,徐志红,徐永阳.2017.甜瓜株型相关性状研究进展.中国瓜菜,30(12):1-3,8.
Zhu Hui-qin.2013.Genetic analysis and QTL mapping for citric acid content,titratable acidity and pH in melon(Cucumis melo L.)fruit[M.D.Dissertation].Beijing:Chinese Academy of Agricultural Sciences.(in Chinese)朱慧芹.2013.甜瓜果实柠檬酸含量、可滴定酸和pH的遗传分析与QTL定位[硕士论文].北京:中国农业科学院.
Zhu H,Guo L,Song P,Luan F,Hu J,Sun X,Yang L.2016.Development of genome-wide SSR markers in melon with their cross-species transferability analysis and utilization in genetic diversity study.Molecular Breeding,36(11):153.
Cho Y G,Eun M Y,Kim Y K,Chung T Y,Chae Y A.1994.The semidwarf gene,sd-1,of rice(Oryza sativa L.).I.Linkage with the esterase locus,Estl-2.Theoretical&Applied Genetics,89(1):49-53.
Ding An-ming,Cui Fa,LI Jun,Zhao Chun-hua,Wang Xiu-qin,Wang Hong-gang.2011.QTL analysis on grain yield per plant and plant height in wheat.Scientia Agricultura Sinica,44(14):2857-2867.
Fazio G,Chung S M,Staub J E.2003.Comparative analysis of response to phenotypic and marker-assisted selection for multiple lateral branching in cucumber(Cucumis sativus L.).Theoretical&Applied Genetics,107(5):875-883.
Fukino N,Ohara T,Sugiyama M,Kubo N,Hirai M,Sakata Y,Matsumoto S.2012.Mapping of a gene that confers short lateral branching(slb)in melon(Cucumis melo L.).Euphytica,187(1):133-143.
Jiang Su,Cai Run,Pan Jun-song,He Huan-le.2009.Anatomy observation and morphogenetic causation of non-lateral branch cucumber line S61.Acta Horticulturae Sinica,36(7):983-988.(in Chinese)蒋苏,蔡润,潘俊松,何欢乐.2009.黄瓜无侧枝品系S61的形态解剖学观察及其无侧枝成因分析.园艺学报,36(7):983-988.
Jiang Su,Yuan Xiao-jun,Pan Jun-song,He Huan-le,Cai Run.2008.Quantitative trait locus analysis of lateral branch-related traits in cucumber(Cucumis sativus L.)using recombinant inbred lines.Science in China Series C:Life Sciences,51(9):833-841.
Li S S,Jia J Z,Wei X Y,Zhang X C,Li L Z,Chen H M,Fan Y D,Sun H Y,Zhao X H,Lei T D,Xu Y F,Jiang F S,Wang H G,Li L H.2007.A intervarietal genetic map and QTL analysis for yield traits in wheat.Molecular Breeding,20:167-178.
Li Xiao-zun,Pan Jun-song,Wang Gang,Tian Li-bo,Si Long-ting,Wu Ai-zhong,Cai Run.2005.Localization of genes for lateral branch and female sex expression and construction of a molecular linkage map in cucumber(Cucumis sativus L.)with RAPD markers.Progress in Natural Science:Materials International,15(2):143-148.
Lu Fang.2009.Construction of permanent genetic map with SSR makers and mapping agronomic traits in hami melon[Cucumis melo L.ssp.melo.convar ameri(Pang.)Greb.][M.D.Dissertation].Harbin:Northeast Agricultural University.(in Chinese)陆芳.2009.哈密瓜SSR永久遗传图谱构建及重要农艺性状定位分析[硕士论文].哈尔滨:东北农业大学.
Ma Hai-cai,Ma Xiong,Liu Jian-li,Gong Hong-dong.2010.Construction of a molecular genetic map for melon with SSR markers.Journal of Fujian Agriculture and Forestry University(Natural Science Edition),39(1):47-52.(in Chinese)马海财,马雄,柳剑丽,巩红冬.2010.利用SSR分子标记构建甜瓜遗传图谱.福建农林大学学报(自然版),39(1):47-52.
Ma H,Zhang S,Ji L,Zhu H,Yang S,Fang X.2006.Fine mapping and in silico,isolation of the EUI1,gene controlling upper internode elongation in rice.Plant Molecular Biology,60(1):87-94.
Ohara T,Wako T,Kojima A,Yoshida,T.Ishiuchi D.2002.Breeding of suppressed-branching melon line‘Melon Chukanbohon Nou 4’(‘Melon parental line 4’)and its characteristics.Acta Horticulturae,588(588):227-231.
Qi Zheng-yu,Li Jun-xing,Zou Xiao-xia,Cao Li-wen,Rao Lin-li,Yu Jin-long,Chen Li-ping.2015.Genetic analysis of plant architecture traits in melon(Cucumis melo L.).Journal of Agricultural Biotechnology,23(3):302-310.(in Chinese)齐振宇,李俊星,邹晓霞,曹丽雯,饶琳莉,郁金龙,陈利萍.2015.甜瓜株型性状的遗传分析.农业生物技术学报,23(3):302-310.
Rebetzke G J,Richards R A.2000.Gibberellic acid-sensitive dwarfing genes reduce plant height to increase kernel number and grain yield of wheat.Australian Journal of Agricultural Research,51(2):235-246.
Ren Guo-liang,Yang Xu-qin,He Huan-le,Cai Run,Pan Jun-song.2013.Genetic mapping of the non-lateral branch gene(nlb)in cucumber.Acta Horticulturae Sinica,40(7):1375-1381.(in Chinese)任国良,杨绪勤,何欢乐,蔡润,潘俊松.2013.黄瓜无侧枝基因nlb的初步定位.园艺学报,40(7):1375-1381.
Serquen F C,Bacher J,Staub J E.1997.Mapping and QTL analysis of horticultural traits in a narrow cross in cucumber(Cucumis sativas L.)using random amplified polymorphic DNA makers.Molecular Breeding,3(4):257-268.
Wang G,Pan J S,Li X,Huanle H E.2005.Construction of a cucumber genetic linkage map with srap markers and location of the genes for lateral branch traits.Science in China,48(3):21-28.
Wei L,Xu J,Li X,Qian Q,Zhu L.2006.Genetic analysis and mapping of the dominant dwarfing gene D-53 in rice.Journal of Integrative Plant Biology,48(4):447-452.
Xing A,Gao Y,Ye L,Zhang W,Cai L,Ching A,Llaca V,Johnson B,Liu L,Yang X,Kang D,Yan J,Li J.2015.A rare SNP mutation in Brachytic2 moderately reduces plant height and increases yield potential in maize.Journal of Experimental Botany,66(13):3791.
Yang L,Koo D,Li D,Zhang T,Jiang J,Luan F,Renner S S,Henaff E,Sanseverino W,Garcia-Mas J,Casacuberta J,Senalik D A,Simon P W,Chen J,Weng Y.2014.Next-generation sequencing,FISH mapping and synteny-based modeling reveal mechanisms of decreasing dysploidy in Cucumis.The Plant Journal,77:16-30.
Zalapa J,Staub J,McCreight J.2008.Variance component analysis of plant architectural traits and fruit yield in melon.Euphytica,162(1):129-143.
Zhang Wei-wei,Pan Jun-song,Jiang Su,He Huan-le,Yu Ping-gao,Cai Run.2017.Genetic mapping of QTL associated with lateral branch-related traits using recombinant inbred lines population in cucumber(Cucumis sativus L.).Jiangsu J of Agr Sci,33(1):174-180.(in Chinese)张微微,潘俊松,蒋苏,何欢乐,俞平高,蔡润.2017.黄瓜RIL群体侧枝相关性状QTL定位.江苏农业学报,33(1):174-180.
Zhang Xue-jiao,Gao Peng,Luan Fei-shi.2013.The QTL analysis of traits related fruits in melon(Cucumis melo L.).China Vegetables,1(18):35-41.(in Chinese)张雪娇,高鹏,栾非时.2013.甜瓜果实相关性状QTL分析.中国蔬菜,1(18):35-41.
Zhang Y,Li Y X,Wang Y,Liu Z Z,Liu C,Peng B,Tan W W,Wang D,Shi Y S,Sun B C,Song Y C,Wang T Y,Li Y.2010.Stability of QTL across environments and QTL-by-Environment interactions for plant and ear height in maize.Agric Sci China,9(10):1400-1412.
Zhao Guang-wei,Xiong Jiang-ling,He Yu-hua,Wang Ping-yong,Kong Wei-hu,Zhang Jian,Xu Zhi-hong,Xu Yong-yang.2017.Research progresses on architecture related traits of melon.China Cucurbits and Vegetables,30(12):1-3,8.(in Chinese)赵光伟,熊姜玲,贺玉花,王平勇,孔维虎,张健,徐志红,徐永阳.2017.甜瓜株型相关性状研究进展.中国瓜菜,30(12):1-3,8.
Zhu Hui-qin.2013.Genetic analysis and QTL mapping for citric acid content,titratable acidity and pH in melon(Cucumis melo L.)fruit[M.D.Dissertation].Beijing:Chinese Academy of Agricultural Sciences.(in Chinese)朱慧芹.2013.甜瓜果实柠檬酸含量、可滴定酸和pH的遗传分析与QTL定位[硕士论文].北京:中国农业科学院.
Zhu H,Guo L,Song P,Luan F,Hu J,Sun X,Yang L.2016.Development of genome-wide SSR markers in melon with their cross-species transferability analysis and utilization in genetic diversity study.Molecular Breeding,36(11):153.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |