补肾、活血中药复方对去卵巢骨质疏松症模型大鼠股骨和肾组织IHH mRNA及蛋白表达的影响
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摘要
目的:观察去卵巢骨质疏松症大鼠模型股骨和肾组织中IHH mRNA和蛋白活性变化,探究中药防治骨质疏松症的分子生物学机制。方法:采用切除雌性SD大鼠双侧卵巢的方式建立骨质疏松症模型,运用补肾中药复方、活血中药复方对模型大鼠进行干预12周,用骨疏康颗粒作为阳性对照药物。RT-PCR检测各组股骨和肾组织IHH mRNA相对表达量;ELISA法检测各组股骨和肾组织IHH蛋白含量。结果:与正常组比较,模型组股骨和肾组织IHH mRNA和蛋白表达量显著减少(P<0.01);与模型组比较,各用药组股骨和肾组织IHH mRNA和蛋白表达均显著增加(P<0.01),且补肾组显著高于活血组(P<0.01)。结论:骨质疏松症的发生机制可能与Hedgehog信号传导通路中IHH含量降低有关;补肾、活血中药复方可以激活IHH信号传导通路,促进骨形成,抑制骨吸收,起到防止骨质疏松症的作用,且补肾中药复方优于活血中药复方。
Objective: To observe the mRNA and protein expression of IHH in bone and kidney tissues of the rats with osteoporosis, and to discuss the molecular biological mechanism of the compound prescription in preventing and treating osteoporosis. Methods: The osteoporosis rat model was established by photosynthesizing the female rats, the osteoporosis rat was treated with experimental compound prescription group for 12 weeks. Gushukang was taken as a positive control drug.Normal control and model control groups were set for comparing. The ELISA and RT-PCR methods were used to investigate the mRNA and protein expression of IHH in bone and kidney tissues. Results: Compared with normal groups, the mRNA and protein expression of IHH in bone and kidney tissues in model control group were significantly decreased(P<0.01). Compared with the model group, the mRNA and protein expression of IHH in bone and kidney tissues in all medicine groups were significantly increased(P<0.01), and the reinforcing kidney group was higher than the activating blood circulation group(P<0.01). Conclusion:The mechanism of osteoporosis may be related to the decrease of IHH content in the Hedgehog signaling pathway. Reinforcing kidney/activating blood circulation compound prescription can activate IHH signaling pathway, promote bone formation, inhibit bone absorption and prevent osteoporosis. Reinforcing kidney compound prescription is superior to activating blood circulation compound prescription compound.
Objective: To observe the mRNA and protein expression of IHH in bone and kidney tissues of the rats with osteoporosis, and to discuss the molecular biological mechanism of the compound prescription in preventing and treating osteoporosis. Methods: The osteoporosis rat model was established by photosynthesizing the female rats, the osteoporosis rat was treated with experimental compound prescription group for 12 weeks. Gushukang was taken as a positive control drug.Normal control and model control groups were set for comparing. The ELISA and RT-PCR methods were used to investigate the mRNA and protein expression of IHH in bone and kidney tissues. Results: Compared with normal groups, the mRNA and protein expression of IHH in bone and kidney tissues in model control group were significantly decreased(P<0.01). Compared with the model group, the mRNA and protein expression of IHH in bone and kidney tissues in all medicine groups were significantly increased(P<0.01), and the reinforcing kidney group was higher than the activating blood circulation group(P<0.01). Conclusion:The mechanism of osteoporosis may be related to the decrease of IHH content in the Hedgehog signaling pathway. Reinforcing kidney/activating blood circulation compound prescription can activate IHH signaling pathway, promote bone formation, inhibit bone absorption and prevent osteoporosis. Reinforcing kidney compound prescription is superior to activating blood circulation compound prescription compound.
引文
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[2]邓洋洋,李佳,孙鑫,等.中医不同治法对绝经后骨质疏松症大鼠骨组织Hedgehog信号通路m RNA和蛋白表达的影响.中国骨质疏松杂志,2017,23(12):1643-1647
[3]Nataliya Razumilava.Indian Hedgehog suppresses intestinal inflammation.Cellular and Molecular Gastroenterology and Hepatology,2018,5(1):63-64
[4]陈晓婷,高艳虹.间充质干细胞成骨分化中多种调控因子的交互作用及其机制.上海交通大学学报(医学版),2017,37(5):694-698
[5]Ye J,Coulouris G,Zaretskaya I,et al.Primer-BLAST:A tool to design target-specific primers for polymerase chain reaction.BMC Bioinformatics,2012,3(1):134
[6]苏麒麟,孙鑫,杨芳,等.补肾中药对绝经后骨质疏松症模型大鼠骨及肌肉组织Notch信号通路蛋白表达的影响.中华中医药杂志,2016,31(8):3208-3212
[7]王剑,刘研,张锦萍,等.鹿蛎中药复方对摘卵巢骨质疏松症大鼠骨组织Leptin蛋白表达的影响.辽宁中医药大学学报,2017,19(12):56-58
[8]高璐,郑洪新,陈谊敬,等.淫羊藿苷、补骨脂素、齐墩果酸、二苯乙烯苷正交配伍调控Bmp2、Smad1、4诱导BMSCs成骨分化的影响.世界科学技术-中医药现代化,2014,16(5):1094-1102
[9]陈易,王明三,徐琬梨.绝经后骨质疏松症证型探讨.辽宁中医杂志,2017,44(2):273-275
[10]张萌萌.中国老年学学会骨质疏松委员会骨代谢生化指标临床应用专家共识.中国骨质疏松杂志,2014,20(11):1263-1272
[11]Briscoe J,Thérond P.The mechanisms of Hedgehog signalling and its roles in development and disease.Nat Rev Mol Cell Biol,2013,14(7):416-429
[12]Carbone A,Carballo C,Ma R,et al.Indian hedgehog signaling and the role of graft tension in tendon-to-bone healing:Evaluation in a rat ACL reconstruction model.J Orthop Res,2016,34(4):641-649