入侵植物胜红蓟精油对小贯小绿叶蝉的忌避和熏蒸活性研究
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摘要
本文研究了入侵植物胜红蓟(Ageratum conyzoides L.)精油对小贯小绿叶蝉(Empoasca onukii Matsuda)的生物活性。分别使用植株选择着落法、Y形嗅觉仪和改良熏蒸法,检测不同浓度的胜红蓟精油对小贯小绿叶蝉的忌避和熏蒸活性。并且采用气质联用仪(GC-MS)分析了胜红蓟精油的化学成分,初步探讨了化学成分与活性的关系。研究结果表明:不同浓度的胜红蓟精油对小贯小绿叶蝉均具有忌避和熏蒸活性,浓度越高,效果越佳。精油含量为200μL·mL~(-1),忌避率达到100.00%;精油含量为100μL·mL~(-1)和50μL·mL~(-1)时,处理小贯小绿叶蝉5 min,忌避率分别为91.67%±1.67%和76.67%±1.67%;精油含量为5μL·mL~(-1)时熏蒸小贯小绿叶蝉12 h,死亡率为100%。并且胜红蓟精油中检测出24种化合物,其主要成分是β-石竹烯(61.53%)、早熟素I(11.09%)和早熟素II(7.79%)。胜红蓟精油对小贯小绿叶蝉的忌避和熏蒸活性可能与其主要成分的协同作用有关。研究结果证明,胜红蓟精油对小贯小绿叶蝉有明显的生物活性,具有开发成防治小贯小绿叶蝉植物源农药的潜力。
The biological activity of essential oil from the invasive plant species Ageratum conyzoides L. to leafhopper Empoasca onukii Matsuda was reported in this study. The effects of essential oil concentrations on E. onukii were also investigated by selecting different plants and using Y-tube olfactometer and modified fumigation methods. The chemical composition of essential oil was examined using gas chromatography-mass spectrometry(GC-MS) and their relative activities was also analyzed. The results indicated that essential oil concentrations from A. conyzoides had significant effects on fumigant and repellent properties against E. onukii. High concentrations of essential oil displayed significant powerful effects. At an oil concentration of 200 μL·m L-1, the repellent rate was 100%. At concentrations of 100 μL·m L-1 and 50 μL·m L-1, the repellent rates were 91.67%±1.67% and 76.67%±1.67%, respectively. At a concentration of 5 μL·m L-1, the fumigant mortality rate at 12 h was 100%. Twenty-four essential oil constituents were identified, with the major constituents being β-caryophyllene(61.53%), precocene I(11.09%), and precocene II(7.79%). Main synergistic composition could effectively increase the repellency and fumigant activity against E. onukii. The results showed that the essential oil of A. conyzoides had significant effects against E. onukii and potential benefits as a botanical pesticide to control E. onukii.
The biological activity of essential oil from the invasive plant species Ageratum conyzoides L. to leafhopper Empoasca onukii Matsuda was reported in this study. The effects of essential oil concentrations on E. onukii were also investigated by selecting different plants and using Y-tube olfactometer and modified fumigation methods. The chemical composition of essential oil was examined using gas chromatography-mass spectrometry(GC-MS) and their relative activities was also analyzed. The results indicated that essential oil concentrations from A. conyzoides had significant effects on fumigant and repellent properties against E. onukii. High concentrations of essential oil displayed significant powerful effects. At an oil concentration of 200 μL·m L-1, the repellent rate was 100%. At concentrations of 100 μL·m L-1 and 50 μL·m L-1, the repellent rates were 91.67%±1.67% and 76.67%±1.67%, respectively. At a concentration of 5 μL·m L-1, the fumigant mortality rate at 12 h was 100%. Twenty-four essential oil constituents were identified, with the major constituents being β-caryophyllene(61.53%), precocene I(11.09%), and precocene II(7.79%). Main synergistic composition could effectively increase the repellency and fumigant activity against E. onukii. The results showed that the essential oil of A. conyzoides had significant effects against E. onukii and potential benefits as a botanical pesticide to control E. onukii.
引文
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[21]刘双弟.不同间作模式对台刈茶园小绿叶蝉及其天敌种群数量的影响[J].中国园艺文摘,2012,6:32-36.
[22]张月.植物精油对假眼小绿叶蝉和烟粉虱的生物活性[D].福州:福建农林大学,2013:39-40.
[23]Feng M G,Pu X Y,Ying S H,et al.Field trials of an oil-based emulsifiable formulation of Beauveria bassiana conidia and low application rates of imidacloprid for control of false-eye leafhopper Empoasca vitis on tea in southern China[J].Crop Protection,2004,23(6):489-496.
[24]Zhang Z Q,Chen Z M.Non-host plant essential oil volatiles with potential for a'push-pull'strategy to control the tea green leafhopper,Empoasca vitis[J].Entomologia Experimentalis et Applicata,2015,156(1):77-87.
[25]施龙清,曾兆华,尤民生,等.室内饲养茶假眼小绿叶蝉的方法:201310212692.1[P].2013-08-28.
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[28]Ziaee M,Moharramipour S,Mohsenifar A.MA-chitosan nanogel loaded with Cuminum cyminum essential oil for efficient management of two stored product beetle pests[J].Journal of Pest Science,2014,87(4):691-699.
[29]lsman M B.Plant essential oils for pest and disease management[J].Crop Protection,2000,19:603-608.
[30]Usman L A,Olawore Z M,Muhammad N O,et al.Chemical constituents of flower essential oil of Ageratum conyzoides growing in Nigeria[J].Elixir Organic Chemistry,2013,54:12463-12465.
[31]赵冬香,徐汉虹,高景林,等.印楝提取物对假眼小绿叶蝉的生物活性研究[J].华南农业大学学报,2003,24(3):30-32.
[32]赵冬香,高景林,徐汉虹.鱼藤根提取物对假眼小绿叶蝉的防治[J].热带作物学报,2005,26(4):86-89.
[33]胡强,韩宝瑜,马建义,等.喜树叶提取物防治假眼小绿叶蝉的田间药效试验[J].中国茶叶,2009,31(5):32-33.
[2]林镕,陈艺林.中国植物志:第74卷[M].北京:科学出版社,1985:53-54.
[3]Kohli R K,Batish D R,Singh H P,et al.Status,invasiveness and environmental threats of three tropical American invasive weeds(Parthenium hysterophorus L.,Ageratum conyzoides L.,Lantana camara L.)in India[J].Biological Invasions,2006,8(7):1501-1510.
[4]Dong H Y,Li Y,Wang Q,et al.Impacts of invasive plants on ecosystems in natural reserves in Jiangsu of China[J].Russian Journal of Ecology,2011,42(2):133-137.
[5]Kong C H,Hu F,Xu X H,et al.Allelopathic plants Ageratum conyzoides L.[J].Allelopathy Journal,2004,14(1):1-12.
[6]郝建华,强胜.外来入侵性杂草—胜红蓟[J].杂草科学,2005(4):54-58.
[7]Bouda H,Tapondjou L A,Fontem D A,et al.Effect of essential oils from leaves of Ageratum conyzoides,Lantana camara and Chromolaena odorata on the mortality ofSitophilus zeamais(Coleoptera,Curculionidae)[J].Journal of Stored Products Research,2001,37(2):103-109.
[8]Boukaew S,Prasertsan P,Sattayasamitsathit S.Evaluation of antifungal activity of essential oils against aflatoxigenic Aspergillus flavus and their allelopathic activity from fumigation to protect maize seeds during storage[J].Industrial Crops and Products,2017,97:558-566.
[9]Nogueira J H C,Gon?alez E,Galleti S R,et al.Ageratum conyzoides essential oil as aflatoxin suppressor of Aspergillus flavus[J].International Journal of Food Microbiology,2010,137(1):55-60.
[10]Bayala B,Bassole I H N,Gnoula C,et al.Chemical composition,antioxidant,anti-inflammatory and anti-proliferative activities of essential oils of plants from Burkina faso[J].Plos One,2014,9(3):e921223.
[11]Esper R H,Gon?alez E,Marques M O M,et al.Potential of essential oils for protection of grains contaminated by aflatoxin produced by Aspergillus flavus[J].Frontiers in Microbiology,2014,5(12):1-5.
[12]Vyas A V,Mulchandani N B.Biosynthesis of precocenes-I and-II,anti-juvenile hormones[J].Phytochemistry,1980,19(12):2597-2598.
[13]Fagoonee I,Umrit G.Anti-gonadotropic hormones from the goatweed,Ageratum conyzoides[J].International Journal of Tropical Insect Science,1981,1(4):373-376.
[14]Jaya P S,Prakash B,Dubey N K.Insecticidal activity of Ageratum conyzoides L.,Coleus aromaticus Benth.andHyptis suaveolens(L.)Poit essential oils as fumigant against storage grain insect Tribolium castaneum Herbst[J].Journal of Food Science and Technology,2014,51(9):2210-2215.
[15]De Melo N I,Magalhaes L G,De Carvalho C E,et al.Schistosomicidal activity of the essential oil of Ageratum conyzoides L.(Asteraceae)against adult Schistosoma mansoni worms[J].Molecules,2011,16(1):762-773.
[16]Moreira M D,Pican?o M C,Barbosa L C,et al.Compounds from Ageratum conyzoides:isolation,structural elucidation and insecticidal activity[J].Pest Management Science,2007,63(6):615-621.
[17]Qin D Z,Zhang L,Xiao Q,et al.Clarification of the identity of the tea green leafhopper based on morphological comparison between Chinese and Japanese specimens[J].Plos One,2015,10(9):e0139202.
[18]王梦馨,李辉仙,武文竹,等.假眼小绿叶蝉对茶梢挥发物的行为反应[J].应用昆虫学报,2016,53(3):507-515.
[19]金珊.不同茶树品种抗假眼小绿叶蝉机理研究[D].杨凌:西北农林科技大学,2012:4.
[20]张海芳.防治假眼小绿叶蝉无公害药剂筛选[D].长沙:湖南农业大学,2010:4.
[21]刘双弟.不同间作模式对台刈茶园小绿叶蝉及其天敌种群数量的影响[J].中国园艺文摘,2012,6:32-36.
[22]张月.植物精油对假眼小绿叶蝉和烟粉虱的生物活性[D].福州:福建农林大学,2013:39-40.
[23]Feng M G,Pu X Y,Ying S H,et al.Field trials of an oil-based emulsifiable formulation of Beauveria bassiana conidia and low application rates of imidacloprid for control of false-eye leafhopper Empoasca vitis on tea in southern China[J].Crop Protection,2004,23(6):489-496.
[24]Zhang Z Q,Chen Z M.Non-host plant essential oil volatiles with potential for a'push-pull'strategy to control the tea green leafhopper,Empoasca vitis[J].Entomologia Experimentalis et Applicata,2015,156(1):77-87.
[25]施龙清,曾兆华,尤民生,等.室内饲养茶假眼小绿叶蝉的方法:201310212692.1[P].2013-08-28.
[26]中华人民共和国家药典委员会.中华人民共和国药典[S].北京:化学工业出版社,2000:720.
[27]Sundufu A J,Huang S S.Chemical composition of the essential oils of Ageratum conyzoides L.occurring in South China[J].Flavour and Fragrance Journal,2004,19(1):6-8.
[28]Ziaee M,Moharramipour S,Mohsenifar A.MA-chitosan nanogel loaded with Cuminum cyminum essential oil for efficient management of two stored product beetle pests[J].Journal of Pest Science,2014,87(4):691-699.
[29]lsman M B.Plant essential oils for pest and disease management[J].Crop Protection,2000,19:603-608.
[30]Usman L A,Olawore Z M,Muhammad N O,et al.Chemical constituents of flower essential oil of Ageratum conyzoides growing in Nigeria[J].Elixir Organic Chemistry,2013,54:12463-12465.
[31]赵冬香,徐汉虹,高景林,等.印楝提取物对假眼小绿叶蝉的生物活性研究[J].华南农业大学学报,2003,24(3):30-32.
[32]赵冬香,高景林,徐汉虹.鱼藤根提取物对假眼小绿叶蝉的防治[J].热带作物学报,2005,26(4):86-89.
[33]胡强,韩宝瑜,马建义,等.喜树叶提取物防治假眼小绿叶蝉的田间药效试验[J].中国茶叶,2009,31(5):32-33.