自发性高血压大鼠微循环障碍与血瘀、出血的相关性研究
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摘要
目的:动态观察自发性高血压大鼠(SHR)视网膜和肠系膜等微循环损伤的变化,分析其与瘀血、出血的相关性,以期为临床诊断及治疗提供实验依据。方法:实验分两组,模型组为雄性SHR 20只,对照组为雄性健康Wistar大鼠20只。采用视网膜照相机及肠系膜微循环观测系统,分别于大鼠6月龄、10月龄时随机各取10只进行检测;同时取血进行血流变学检测。结果:6月龄与10月龄模型组大鼠均可见视网膜及肠系膜微血管形态异常、数量减少、血液流速明显减慢、微动脉变细、DA/DV明显减小等瘀血反应,与对照组相比,差异均具有统计学意义(P<0.05或P<0.01);随着月龄的增加,血压增高,瘀血损伤反应加重,出现微血管瘤及出血等改变。结论:随着病程的改变,自发性高血压大鼠可出现血瘀、出血的病理改变。
Objective:To dynamically observe microcirculation injuries of retina and mesentery in the spontaneously hypertensive rats(SHRs),to analysis whether blood stasis and bleeding is correlated with microcirculation disturbance,and to provide the experimental evidence for clinical diagnosis and treatment. Methods:Rats were divided into the control and model groups. In the model group there were 20 male SHRs and in the control group there were 20 male healthy Wistar rats. Retinal camera and mesenteric microcirculation observation system were used to perform the observation. Ten rats from each group were randomly selected for the observation and hemorheological examination at the age of 6 months and 10 months. Results:In the model group the rats at the age of 6 months and 10 months showed the manifestation of blood stasis,such as dysmorphic retinal and mesenteric microvessels,decreased microvessels,significantly decreased blood flow,narrowing of microartery,decreased DA/DV,with statistical difference compared with the control group rats(P<0.05 or P<0.01). With the increase of age,blood pressure increased,injury due to blood stasis became serious,and even microangioma and bleeding appeared. Conclusions:With the development of the disease,SHRs could manifest pathological changes like blood stasis and hemorrhage.
Objective:To dynamically observe microcirculation injuries of retina and mesentery in the spontaneously hypertensive rats(SHRs),to analysis whether blood stasis and bleeding is correlated with microcirculation disturbance,and to provide the experimental evidence for clinical diagnosis and treatment. Methods:Rats were divided into the control and model groups. In the model group there were 20 male SHRs and in the control group there were 20 male healthy Wistar rats. Retinal camera and mesenteric microcirculation observation system were used to perform the observation. Ten rats from each group were randomly selected for the observation and hemorheological examination at the age of 6 months and 10 months. Results:In the model group the rats at the age of 6 months and 10 months showed the manifestation of blood stasis,such as dysmorphic retinal and mesenteric microvessels,decreased microvessels,significantly decreased blood flow,narrowing of microartery,decreased DA/DV,with statistical difference compared with the control group rats(P<0.05 or P<0.01). With the increase of age,blood pressure increased,injury due to blood stasis became serious,and even microangioma and bleeding appeared. Conclusions:With the development of the disease,SHRs could manifest pathological changes like blood stasis and hemorrhage.
引文
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[23]Nazzaro P,Vulpis V,Schirosi G,et al.Microvascular impeirment is associated wigh insulin resistance in euglycemic mild hypertrnsives[J].Hypertens,2008,221(4):432-437.
[24]秦林,彭欣,王栋先.血瘀证与出血证的辨治关系与特点[J].山东中医药大学学报,2015,39(4):299-300.
[2]孙静,李宝珍,李英.论活血化瘀药物在高血压病治疗中的应用[J].中国民间疗法,2012,20(1):63-64.
[3]van Es ACGM,van der Grond J,de Craen A J M,et al.Cerebral microbleeds and cognitive functioning in the PROSPER study[J].Neurology,2011,7(15):1446-1452.
[4]李婷,张微微.脑微出血的研究新进展[J].北京医学,2015,37(1):41-43.
[5]郭静萱,刘健.抗栓治疗出血与缺血的平衡[J].中国循环杂志,2011,26(1):1-3.
[6]袁晋青,张佳慧.血栓与出血[J].中国实用内科杂志,2015,35(1):18-23.
[7]陈小燕,郑碧辉,韦瑞焕.高血压病Ⅱ期甲襞微循环与中医血瘀证[J].光明中医,2001,16(5):21-23.
[8]杨瑞华,骆秉铨,陈兴新,等.自发性高血压大鼠微循环变化及异搏定的干预[J].微循环技术杂志,1994(2):87-91.
[9]吴秀川,牟琴,于志瀛.尿毒清对老龄自发性高血压大鼠血压、血脂及血黏度的影响[J].中国中西医结合肾病杂志,2014,15(9):800-802.
[10]江时森,顾小萍,李辉,等.高血压患者和高血压鼠视网膜微血管稀疏状态的定量研究[J].金陵医院学报,1997,10(1):50-54.
[11]张方申,彭欣,秦林,等.痰瘀互结型动脉粥样硬化大鼠视网膜和肠系膜微循环观察[J].微循环学杂志,2015,25(1):10-13+22+83.
[12]孟凡星.自发性高血压大鼠肠系膜微循环的改变及内皮细胞对血管钙化的调节作用研究[D].北京:北京协和医学院,2015:18-19.
[13]刘育英.微循环图谱[M].北京:人民军医出版社,2005:8.
[14]Sanada S,Komuro I,Kitakaze M.Pathophysiology of myocardial reperfusion injury:preconditioning,postconditioning,and translational aspects of protective measures[J].Am J Physiol Heart Circ Physiol,2011,301(5):1723-1741.
[15]李丽琴,郭竹秀,吴锐.微循环改变与血液高凝状态在血瘀证中的研究进展[J].现代中西医结合杂志,2010,19(35):4640-4641.
[16]胡文娟,张秉韬,吴锐.5种血瘀证亚型家兔模型球结膜微循环改变的比较[J].中国中西医结合杂志2013,33(9):1260-1266.
[17]胡庆全.脑梗塞中医证型与微循环关系研究[J].中国微循环,2001,5(4):306-307.
[18]Ferrieira R.The reduction of infarct size forty years of research[J].Rev port cardiol,2010,9(6):1037-1053.
[19]何涛,陶天琪,李玉珍,等.不同危险分层原发性高血压病患者甲襞微循环改变[J].微循环学杂志,2015,25(3):17-22.
[20]张海燕,张方申,秦林,等.血瘀出血兼证的现代临床诊治研究概况[J].现代中西医结合杂志,2015,24(25):2842-2845.
[21]彭欣,秦林.血虚证“瘀血出血”微观病机探究[J].山东中医杂志,2012,31(6):387-388.
[22]王彦芳,彭欣,秦林.化瘀止血法临床应用概述[J].山东中医药大学学报,2012,36(12):361-363.
[23]Nazzaro P,Vulpis V,Schirosi G,et al.Microvascular impeirment is associated wigh insulin resistance in euglycemic mild hypertrnsives[J].Hypertens,2008,221(4):432-437.
[24]秦林,彭欣,王栋先.血瘀证与出血证的辨治关系与特点[J].山东中医药大学学报,2015,39(4):299-300.