不同免疫学检验方法对混合精斑的检测及应用
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摘要
目的利用免疫学检验方法对混合精斑进行分离确证,寻找更有效的混合精斑检测方法。方法将3名志愿者的精液混合后,分别用前列腺特异性抗原(prostate-specific antigen,PSA)胶体金免疫试纸条法、免疫磁珠法以及激光捕获显微切割技术对混合精斑进行处理和检测,对分型结果进行统计,并与单一精斑结果进行比较。结果经PSA胶体金免疫试纸条法测试,各样品在测试区和控制区内均出现一条紫红色条带;经免疫磁珠法处理后,检出更完整有效的短串联重复(short tandem repeat,STR)分型;应用激光捕获显微切割技术对混合精斑进行处理并经低体积扩增后,将结果汇总、叠加获得完整的单一分型,与单一精斑分型相匹配,分型成功率为84.00%。把上述方法应用于实际案件中,获得了单一的嫌疑人Y-STR分型,为案件的快速破获提供了证据基础。结论联合应用PSA胶体金免疫试纸条法、免疫磁珠法以及激光捕获显微切割技术可快速对混合精斑进行分离检验。
Objective To perform the separation and confirmation of mixed semen stains with immunological test method, and find a more effective method for the detection of mixed semen stains. Methods The semens of three volunteers were mixed. The mixed semen stains were processed and tested with prostate-specific antigen(PSA)colloidal gold immunoassay strip method, immunomagnetic beads method and laser capture microdissection, respectively. Statistics of the results of STR were gathered and compared with those of a single semen stain. Results After PSA colloidal gold immunoassay strip method testing, the samples showed a purplish red line in the test area and the control area. The results obtained with the immunomagnetic beads method showed a more complete and effective short tandem repeat(STR)sequence. The mixed semen stains were processed with laser capture microdissection and low volume amplified. The results were summarized and superimposed to obtain a complete single typing,which matched the single semen stain typing, with a typing success rate of 84.00%. Single suspect YSTR typing was obtained with the application of the method above in actual cases, which provided evidence basis for rapid solving of the case. Conclusion The combination of PSA colloidal gold immunoassay strip method, immunomagnetic beads method and laser capture microdissection can be used to separate and confirm the mixed semen stains.
Objective To perform the separation and confirmation of mixed semen stains with immunological test method, and find a more effective method for the detection of mixed semen stains. Methods The semens of three volunteers were mixed. The mixed semen stains were processed and tested with prostate-specific antigen(PSA)colloidal gold immunoassay strip method, immunomagnetic beads method and laser capture microdissection, respectively. Statistics of the results of STR were gathered and compared with those of a single semen stain. Results After PSA colloidal gold immunoassay strip method testing, the samples showed a purplish red line in the test area and the control area. The results obtained with the immunomagnetic beads method showed a more complete and effective short tandem repeat(STR)sequence. The mixed semen stains were processed with laser capture microdissection and low volume amplified. The results were summarized and superimposed to obtain a complete single typing,which matched the single semen stain typing, with a typing success rate of 84.00%. Single suspect YSTR typing was obtained with the application of the method above in actual cases, which provided evidence basis for rapid solving of the case. Conclusion The combination of PSA colloidal gold immunoassay strip method, immunomagnetic beads method and laser capture microdissection can be used to separate and confirm the mixed semen stains.
引文
[1]侯一平.法医物证学[M].3版.北京:人民卫生出版社,2009:117-118.
[2]范光耀,赵贵森,莫耀南.精斑鉴别技术研究进展[J].中华男科学杂志,2010,16(8):735-740.
[3]李慧,姚亚飞.性侵犯案件中对于精斑试纸条使用灵敏度以及特异性的研究[J].法制与社会,2012(9):258-259.
[4]杨电,刘超,徐曲毅,等.DNA IQ磁珠法结合MaxwellTM16自动仪提取接触DNA[J].刑事技术,2011(3):3-5.
[5]张健,胡兰,张惠芹,等.利用LCM技术从生物检材中提取单个细胞[J].中国人民公安大学学报(自然科学版),2005(1):14-17.
[6]喻少波,许飞.混合斑检材中极微量精子DNA检验初探[J].警察技术,2012(2):18-19.
[2]范光耀,赵贵森,莫耀南.精斑鉴别技术研究进展[J].中华男科学杂志,2010,16(8):735-740.
[3]李慧,姚亚飞.性侵犯案件中对于精斑试纸条使用灵敏度以及特异性的研究[J].法制与社会,2012(9):258-259.
[4]杨电,刘超,徐曲毅,等.DNA IQ磁珠法结合MaxwellTM16自动仪提取接触DNA[J].刑事技术,2011(3):3-5.
[5]张健,胡兰,张惠芹,等.利用LCM技术从生物检材中提取单个细胞[J].中国人民公安大学学报(自然科学版),2005(1):14-17.
[6]喻少波,许飞.混合斑检材中极微量精子DNA检验初探[J].警察技术,2012(2):18-19.