淫羊藿苷对IL-1β诱导的炎症性软骨细胞表型与代谢的影响

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英文篇名：Effects of icariin on phenotype and metabolism in IL-1β-induced inflammatory chondrocytes 作者：李安琪 ; 刘益杰 ; 冯伟 ; 何东仪 ; 张增乔 ; 胡笑燊 ; 杨松滨 ; 张彩 英文作者：LI Anqi;LIU Yijie;FENG Wei;HE Dongyi;ZHANG Zengqiao;HU Xiaoshen;YANG Songbin;ZHANG Cai;School of Rehabilitation Medicine,Shanghai University of Traditional Chinese Medicine;Shanghai Guanghua Hospital of Integrated Traditional Chinese and Western Medicine; 关键词：淫羊藿苷 ; 骨关节炎 ; 软骨细胞 英文关键词：icariin;;osteoarthritis;;chondrocytes 中文刊名：SHZZ 英文刊名：Shanghai Journal of Traditional Chinese Medicine 机构：上海中医药大学康复医学院;上海市光华中西医结合医院; 出版日期：2018-02-09 出版单位：上海中医药杂志 年：2018 期：v.52;No.577 基金：国家自然科学基金资助项目(81774114);; 上海市自然基金资助项目(15ZR1441000);; 上海市卫计委进一步加快中医药事业发展三年行动计划资助项目(ZY3-LCPT-1);上海市卫计委科研课题计划资助项目(201640192) 语种：中文; 页：SHZZ201802029 页数：5 CN：02 ISSN：31-1276/R 分类号：105-109

摘要

目的探讨淫羊藿苷(Icariin,ICA)对IL-1β诱导的炎症软骨细胞表型的影响。方法分离培养大鼠关节软骨细胞,利用白细胞介素-1β(interleukin-1β,IL-1β)诱导原代软骨细胞,模拟骨关节炎软骨细胞炎症内坏境。实验分为空白组、模型组(含IL-1β10 mg/L)、ICA低浓度组(含10 mg/L IL-1β和1μmol/L ICA)和ICA高浓度组(含10 mg/L IL-1β和5μmol/L ICA)。利用Western blot的方法检测II型胶原蛋白(collagen-II,Col-II)和蛋白聚糖(aggrecan)蛋白表达情况,利用RT-PCR法检测collagen-II、aggrecan、MMP-13、ADAMTS-5 m RNA表达情况。结果培养3天时,与模型组比较,ICA对炎症软骨细胞collagen-II m RNA和蛋白表达有明显的促进作用(P<0.05),ICA高浓度组对collagen-II m RNA和蛋白表达的促进作用较低浓度组更强(P<0.05);与模型组比较,ICA对炎症软骨细胞aggrecan m RNA和蛋白表达有明显的促进作用(P<0.05),不同浓度的ICA促进作用差异不明显。与模型组比较,ICA可抑制MMP-13、ADAMTS-5 m RNA表达(P<0.05),ICA高浓度组抑制MMP-13 m RNA表达作用更显著(P<0.05),不同浓度ICA对ADAMTS-5 m RNA表达差异不明显。结论 ICA可以通过抑制MMP-13、ADAMTS-5的表达,促进炎症软骨细胞collagen-II和aggrecan的表达,从而维持软骨细胞表型和促进基质代谢。
        Objective To investigate the effects of icariin( ICA) on the phenotype of interleukin-1β( IL-1β)-induced inflammatory chondrocytes. Methods The articular chondrocytes of rats were isolated and cultured. The primary chondrocytes were induced by IL-1β,and the inflammatory environment of chondrocytes in osteoarthritis was simulated. The cells were divided into the blank group,model group( treated with IL-1β at concentration of 10 mg/L),ICA group with lowconcentration( treated with IL-1β at concentration of 10 mg/L and ICA at concentration of 1 μmol/L) and ICA group with high-concentration( treated with IL-1β at concentration of 10 mg/L and ICA at concentration of 5 μmol/L).The protein expressions of collagen-II and aggrecan were detected by Western blot. The m RNA expressions of collagen-II,aggrecan,matrix metallopeptidase-13( MMP-13) and a disintegrin and metalloprotease with thrombospondin motifs-5( ADAMTS-5) were detected by RT-PCR. Results After the cells were cultured for three day,compared with the model group,ICA could obviously promote the m RNA and protein expressions of collagen-II in the inflammatory chondrocytes( P<0.05); and this effect in the ICA group with high-concentration was more significant than that in the ICA group with low-concentration( P<0.05). Compared with the model group,ICA could obviously promote the m RNA and protein expressions of aggrecan in the inflammatory chondrocytes( P<0.05); and this effect shows no obviously difference between the low-and high-concentration. Compared with the model group,ICA could inhibit the m RNA expressions of MMP-13 and ADAMTS-5( P<0.05),and the inhibiting effect on MMP-13 m RNA expression was more significant in the ICA group with high-concentration( P < 0.05); there was no obvious difference on the ADAMTS-5 m RNA expression between the low-and high-concentration.Conclusion ICA can inhibit the expressions of MMP-13 and ADAMTS-5 and promote the expressions of collagen-II and aggrecan in the inflammatory chondrocytes,and thus maintain the chondrocytes phenotype and promote the matrix metabolism.

引文

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