不同来源白及中3个主要化学成分的含量比较
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摘要
为了了解相同种植条件下60个不同来源白及中贝母兰宁、山药素Ⅲ和3'-O-甲基山药素Ⅲ的含量差异。该研究采用UPLC进行测定,采用Waters ACQUITY UPLC BEH C18(2. 1 mm×100 mm,1. 7μm)色谱柱,以乙腈-0. 1%甲酸水为流动相进行梯度洗脱,流速为0. 208 m L·min-1,检测波长270 nm,柱温35℃,进样量为4μL。结果表明,在上述色谱条件下3个成分分离度良好,在0. 156～5. 000 mg·L-1线性良好,测得贝母兰宁、山药素Ⅲ和3'-O-甲基山药素Ⅲ平均质量分数分别达(0. 116±0. 071)%,(0. 386±0. 185)%,(0. 086±0. 034)%。不同来源、不同品种白及在相同条件下种植2年后,化学组成差异不大,但3个成分含量具有一定的地域性差异,表现为西部地区高于东部地区的趋势;而华白及中贝母兰宁、山药素Ⅲ含量稍高于白及。该色谱方法简单,稳定性、重复性好,可用于3个成分的定量分析;不同来源白及成分含量分析可为今后白及优良品种选育和质量标准制定提供参考。
In order to understand the difference of contents of coelonin,batatasin Ⅲ and 3'-O-methylbatatasin Ⅲ in 60 different sources of Bletilla striata planted under the same conditions. UPLC method was used and the analysis was performed on a Waters ACQUITY UPLC BEH C18 column( 2. 1 mm×100 mm,1. 7 μm),eluted with acetonitril-0. 1% formic acid solution by gradient. The flow rate was 0. 208 m L·min-1,the detection wavelength was 270 nm,the column temperature was 35 ℃ and the injection volume was 4μL. Under the above chromatographic conditions,the three components can be separated well with good linearity in the range of 0. 156-5. 000 mg·L-1. The average contents of coelonin,batatasin Ⅲ and 3'-O-methylbatatasin Ⅲ were( 0. 116 ± 0. 071) %,( 0. 386 ±0. 185) % and( 0. 086±0. 034) %,respectively. After planting for two years under the same conditions,there was no significant difference in chemical composition among different sources and varieties,but the contents of the three components had some regional differences,which indicated that the western region was higher than the eastern region,while the contents of coelonin and batatasin Ⅲ in B.sinensis were slightly higher than those in B. striata. The chromatographic method above is simple,stable and reproducible,and can be used for quantitative analysis of three components. The content analysis of different sources of B. striata can provide reference for future B. striata breeding and quality control.
In order to understand the difference of contents of coelonin,batatasin Ⅲ and 3'-O-methylbatatasin Ⅲ in 60 different sources of Bletilla striata planted under the same conditions. UPLC method was used and the analysis was performed on a Waters ACQUITY UPLC BEH C18 column( 2. 1 mm×100 mm,1. 7 μm),eluted with acetonitril-0. 1% formic acid solution by gradient. The flow rate was 0. 208 m L·min-1,the detection wavelength was 270 nm,the column temperature was 35 ℃ and the injection volume was 4μL. Under the above chromatographic conditions,the three components can be separated well with good linearity in the range of 0. 156-5. 000 mg·L-1. The average contents of coelonin,batatasin Ⅲ and 3'-O-methylbatatasin Ⅲ were( 0. 116 ± 0. 071) %,( 0. 386 ±0. 185) % and( 0. 086±0. 034) %,respectively. After planting for two years under the same conditions,there was no significant difference in chemical composition among different sources and varieties,but the contents of the three components had some regional differences,which indicated that the western region was higher than the eastern region,while the contents of coelonin and batatasin Ⅲ in B.sinensis were slightly higher than those in B. striata. The chromatographic method above is simple,stable and reproducible,and can be used for quantitative analysis of three components. The content analysis of different sources of B. striata can provide reference for future B. striata breeding and quality control.
引文
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[23]吴梅,李宏哲,薛咏梅,等.小白及高效液相指纹图谱分析[J].中国实验方剂学杂志,2019,25(5):180.
[2]刘逢芹,余晓东.白及栓塞剂在抗肿瘤中应用[J].时珍国医国药,1999,10(4):298
[3]佚名.白芨治疗矽肺效果好[J].中药通报,1958(11):384.
[4]康庄,李洪芳,王和,等.中药白及凝胶治疗肺外结核及空洞型肺结核的临床观察[J].河北医药,2012,34(11):1731.
[5]陆春芳.中药材白及质量标准研究[D].苏州:苏州大学,2014.
[6]赵艳霞,邓雁如,张晓静,等.白及属药用植物化学成分及药理作用研究进展[J].天然产物研究与开发,2013,25(8):1137.
[7]王巍,夏令强,陈君,等. LC-MS/MS测定白及止血海绵中白及葡甘聚糖的含量[J].第二军医大学学报,2014,35(12):1401.
[8]代建丽,周斯荻,刘铭轩,等.白芨多糖的抑菌作用研究[J].安徽农学通报,2015,21(19):19.
[9] Luo Y,Diao H,Xia S,et al. A physiologically active polysaccharide hydrogel promotes wound healing[J]. J Biomed Mater Res A,2010,94A(1):193.
[10]韩丹.白芨多糖的提取分离、分子量测定及结构研究[D].延吉:延边大学,2013.
[11]吴久健,孟岳良,邹俪华,等.白及不同提取部位对小鼠止血活性实验[J].药学实践杂志,2011,29(3):206.
[12]刘星星.白及止血作用、作用机制及止血效价测定研究[D].贵阳:贵阳医学院,2015.
[13]彭芙,万峰,熊亮,等.白及抑菌作用及其活性部位的初步研究[J].时珍国医国药,2013,24(5):1061.
[14]方应权,全哲山.白及正丁醇萃取物对胃溃疡作用研究[J].江西中医药,2008,39(11):69.
[15]戴鸥,仰莲,周勤梅,等.白及化学成分分离鉴定[J].中国实验方剂学杂志,2018,24(14):43.
[16]何迅,王爱民,李勇军,等.HPLC测定白及中militarine含量[J].中国中药杂志,2009,34(16):2076.
[17]张晓静,赵艳霞,邓雁如,等.HPLC同时测定白及中3种成分的含量[J].中国实验方剂学杂志,2015,21(21):40.
[18]梅朝叶,向文英,杨武,等.UPLC-MS/MS同时测定白及中6个指标成分的含量[J].天然产物研究与开发,2016(8):1233.
[19]邓延珍,金丽霞,高承贤,等.白及小分子组分抗矽肺大鼠肺纤维化活性研究[J].中药材,2016,39(11):2615.
[20]蒋福升,李美芽,丁滨,等.一种白及有效部位及其应用:中国.201810696274. 1[P]. 2018-06-29.
[21]李浩宇,史珍珍,舒立峰,等.白及多糖抗矽肺大鼠肺纤维化活性研究[J].中药材,2016,39(7):1638.
[22]邓延珍.基于RAW264. 7巨噬细胞炎症模型探讨白及抗肺纤维化活性组分及分子机理[D].杭州:浙江中医药大学,2017.
[23]吴梅,李宏哲,薛咏梅,等.小白及高效液相指纹图谱分析[J].中国实验方剂学杂志,2019,25(5):180.