广藿香酮对分泌性中耳炎模型豚鼠耳黏膜中TNF-α及ICAM-1的表达影响研究
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摘要
目的:探讨广藿香酮对分泌性中耳炎模型豚鼠耳黏膜中肿瘤坏死因子-α(TNF-α)及细胞间黏附分子(ICAM-1)表达的影响。方法:选取健康豚鼠82只,雌雄各半,随机分为对照组16只和造模组66只。造模组豚鼠通过灭活细菌诱导法建立分泌性中耳炎模型,对照组则给予等量生理盐水注射。将造模成功豚鼠随机分为模型组、广藿香酮低、高剂量组以及泼尼松组,各16只。模型组和对照组分别灌胃给予对照溶液2.5 mL,1次/d;广藿香酮低、高剂量组分别灌胃给予1 mg/mL、4 mg/mL广藿香酮溶液2.5 mL,1次/d;泼尼松组灌胃给予0.8 g/mL泼尼松溶液2.5 mL,1次/d。各组豚鼠均连续给药7 d。检测听性脑干反应(ABR)指标、中耳黏膜厚度及中性粒细胞计数、耳泡灌洗液TNF-α、可溶性细胞间黏附分子(sICAM-1)水平、耳黏膜TNF-α、ICAM-1表达水平。结果:治疗后,与模型组比较,各组豚鼠刺激强度90 dB时ABRⅠ、Ⅲ波潜伏期较短,ABR反应阈较低,各组豚鼠中耳黏膜厚度及中性粒细胞计数较低,各组豚鼠耳泡灌洗液TNF-α、sICAM-1水平较低,各组豚鼠中耳黏膜TNF-α、ICAM-1灰度值较低,且广藿香酮低剂量组>广藿香酮高剂量组、泼尼松组,差异有统计学意义(P<0.05)。结论:广藿香酮能明显减轻分泌性中耳炎模型豚鼠听力损失,减轻黏膜厚度和中性粒细胞浸润,其作用可能与抑制耳黏膜中TNF-α及ICAM-1表达有关。
Objective: To analyze the effect of patchouli ketone on the expressions of tumor necrosis factor-α(TNF-α) and intercellular adhesion molecule(ICAM-1) in the ear mucous membrane of guinea pigs with secretory otitis media. Methods: Eighty-two healthy guinea pigs, sex in half, were randomly divided into control group with 16 guinea pigs and model building group with 66 guinea pigs. The model of secretory otitis media was established by inactivated bacterial induction. The control group was given equal amount of saline injection. The successfully modeled guinea pigs were randomly divided into model group, patchouli ketone low-dose, high-dose groups and prednisone group, each with 16 guinea pigs. The model group and the control group were respectively given the control solution 2.5 mL by gavage, once a day. The patchouli ketone low-dose, high-dose groups were respectively given the 1 mg/mL, 4 mg/mL patchouli ketone solution 2.5 mL by gavage, once a day. The prednisone group was given 0.8 g/mL prednisone solution 2.5 mL by gavage, once a day. Each group was given a continuous dose for 7 days. The indexes of auditory brainstem response(ABR), the thickness of the middle ear mucosa and the count of neutrophils, the levels of TNF-α and soluble intercellular adhesion molecule(sICAM-1) in the alveolar lavage fluid,expression levels of TNF-α and ICAM-1 in the ear mucosa were detected. Results: After treatment, compared with model group, the latencies of ABR I and III wave at 90 dB of stimulation intensity were shorter, ABR reaction threshold was lower in each group and the thickness of the middle ear mucosa and the count of neutrophils in the middle ear of the guinea pigs were lower in each group. The levels of TNF-α and sICAM-1 in the alveolar lavage liquid of guinea pig in each group were lower. The gray values of TNF-α and ICAM-1 in the middle ear mucosa of the guinea pigs were lower in each group and that of the patchouli ketone low-dose was more than that of high-dose group and prednisone group. The difference was statistically significant(P<0.05). Conclusions: Patchouli ketone can significantly reduce the loss of hearing in the secretory otitis media model of guinea pigs, the thickness of mucous membrane and neutrophil infiltration, and the effect may be related to the inhibition of TNF-αand ICAM-1 expression in the ear mucous membrane.
Objective: To analyze the effect of patchouli ketone on the expressions of tumor necrosis factor-α(TNF-α) and intercellular adhesion molecule(ICAM-1) in the ear mucous membrane of guinea pigs with secretory otitis media. Methods: Eighty-two healthy guinea pigs, sex in half, were randomly divided into control group with 16 guinea pigs and model building group with 66 guinea pigs. The model of secretory otitis media was established by inactivated bacterial induction. The control group was given equal amount of saline injection. The successfully modeled guinea pigs were randomly divided into model group, patchouli ketone low-dose, high-dose groups and prednisone group, each with 16 guinea pigs. The model group and the control group were respectively given the control solution 2.5 mL by gavage, once a day. The patchouli ketone low-dose, high-dose groups were respectively given the 1 mg/mL, 4 mg/mL patchouli ketone solution 2.5 mL by gavage, once a day. The prednisone group was given 0.8 g/mL prednisone solution 2.5 mL by gavage, once a day. Each group was given a continuous dose for 7 days. The indexes of auditory brainstem response(ABR), the thickness of the middle ear mucosa and the count of neutrophils, the levels of TNF-α and soluble intercellular adhesion molecule(sICAM-1) in the alveolar lavage fluid,expression levels of TNF-α and ICAM-1 in the ear mucosa were detected. Results: After treatment, compared with model group, the latencies of ABR I and III wave at 90 dB of stimulation intensity were shorter, ABR reaction threshold was lower in each group and the thickness of the middle ear mucosa and the count of neutrophils in the middle ear of the guinea pigs were lower in each group. The levels of TNF-α and sICAM-1 in the alveolar lavage liquid of guinea pig in each group were lower. The gray values of TNF-α and ICAM-1 in the middle ear mucosa of the guinea pigs were lower in each group and that of the patchouli ketone low-dose was more than that of high-dose group and prednisone group. The difference was statistically significant(P<0.05). Conclusions: Patchouli ketone can significantly reduce the loss of hearing in the secretory otitis media model of guinea pigs, the thickness of mucous membrane and neutrophil infiltration, and the effect may be related to the inhibition of TNF-αand ICAM-1 expression in the ear mucous membrane.
引文
[1] 任妍妍,何双八,于振坤.分泌性中耳炎病因研究及治疗进展[J].国际耳鼻咽喉头颈外科杂志,2017,41(3):132-135.
[2] 刘娅,杨军,张杰,等.临床实践指南:分泌性中耳炎(更新版)[J].听力学及言语疾病杂志,2016,24(5):499-519.
[3] 洪超群,吴亚庆.加味参苓白术散治疗儿童分泌性中耳炎的临床疗效观察[J].中国中医药科技,2017,24(3):375-376.
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[5] 唐正伟,彭成,王蓬勃,等.广藿香油治疗急性中耳炎模型豚鼠的实验探讨[J].中国实验方剂学杂志,2015,21(18):144-148.
[6] 李卓豪,徐果,张志钢.分泌性中耳炎动物模型建立方法及研究进展[J].中华耳科学杂志,2015,13(4):757-760.
[7] 程馨,杨驹,吴楷文,等.水合氯醛对豚鼠听性脑干反应的影响[J].听力学及言语疾病杂志,2017,25(2):171-175.
[8] 秦川.实验动物学[M].北京:人民卫生出版社,2010:274-278.
[9] 常向辉.中西医结合疗法在分泌性中耳炎患者中的应用研究[J].中西医结合研究,2016,8(2):92-93.
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[11] 刘光华.分泌性中耳炎治疗新进展[J].中国当代医药,2016,23(26):12-14.
[12] 李大鹏,黄辉,何苗,等.分泌性中耳炎的临床诊治进展[J].中华耳科学杂志,2017,15(1):105-109.
[13] 徐雯,吴艳清,丁浩然,等.广藿香的药理作用及机制研究进展[J].上海中医药杂志,2017,1(10):103-106.
[14] Peng F,Wan F,Xiong L,et al.In vitro and in vivo antibacterial activity of Pogostone[J].Chin Med J(Engl Ed),2014,127(23):4001-4005.
[15] 黎玉翠.广藿香酮及广藿香醇的抗炎、抗真菌活性及药物代谢研究[D].广州:广州中医药大学,2013:132-136.
[16] 齐珊珊,胡丽萍.广藿香叶挥发油抗炎作用机制实验研究[J].中国实用医药,2015,10(2):249-251.
[17] 何兴丽,冉文婧,王永义,等.主客观听力检测相关性研究进展[J].中国工业医学杂志,2017(1):29-32.
[18] 赵兴泉,钟渠,陈茜.分泌性中耳炎研究现状[J].国际耳鼻咽喉头颈外科杂志,2017,41(3):136-140.
[19] 王梦航,李水静,韩琳,等.MUC5AC和TNF-α在大鼠急性分泌性中耳炎模型中的表达变化[J].中华耳科学杂志,2017,15(3):329-333.
[20] 迟作华,刘振,肖平.Tregs及IL-6、IL-10、TNF在急性分泌性中耳炎患者中的变化及意义[J].实用医学杂志,2016,32(2):255-257.
[21] 马慧琴,张治军.分泌性中耳炎相关细胞因子免疫机制研究进展[J].山东大学耳鼻喉眼学报,2012,26(4):78-81.
[2] 刘娅,杨军,张杰,等.临床实践指南:分泌性中耳炎(更新版)[J].听力学及言语疾病杂志,2016,24(5):499-519.
[3] 洪超群,吴亚庆.加味参苓白术散治疗儿童分泌性中耳炎的临床疗效观察[J].中国中医药科技,2017,24(3):375-376.
[4] Cole LK,Samii VF,Wagner SO,et al.Diagnosis of primary secretory otitis media in the cavalier King Charles spaniel[J].Veterinary Dermatology,2015,26(6):459-507.
[5] 唐正伟,彭成,王蓬勃,等.广藿香油治疗急性中耳炎模型豚鼠的实验探讨[J].中国实验方剂学杂志,2015,21(18):144-148.
[6] 李卓豪,徐果,张志钢.分泌性中耳炎动物模型建立方法及研究进展[J].中华耳科学杂志,2015,13(4):757-760.
[7] 程馨,杨驹,吴楷文,等.水合氯醛对豚鼠听性脑干反应的影响[J].听力学及言语疾病杂志,2017,25(2):171-175.
[8] 秦川.实验动物学[M].北京:人民卫生出版社,2010:274-278.
[9] 常向辉.中西医结合疗法在分泌性中耳炎患者中的应用研究[J].中西医结合研究,2016,8(2):92-93.
[10] 何腾,胡文健.分泌性中耳炎病因及发病机制研究进展[J].现代医药卫生,2015(5):705-707.
[11] 刘光华.分泌性中耳炎治疗新进展[J].中国当代医药,2016,23(26):12-14.
[12] 李大鹏,黄辉,何苗,等.分泌性中耳炎的临床诊治进展[J].中华耳科学杂志,2017,15(1):105-109.
[13] 徐雯,吴艳清,丁浩然,等.广藿香的药理作用及机制研究进展[J].上海中医药杂志,2017,1(10):103-106.
[14] Peng F,Wan F,Xiong L,et al.In vitro and in vivo antibacterial activity of Pogostone[J].Chin Med J(Engl Ed),2014,127(23):4001-4005.
[15] 黎玉翠.广藿香酮及广藿香醇的抗炎、抗真菌活性及药物代谢研究[D].广州:广州中医药大学,2013:132-136.
[16] 齐珊珊,胡丽萍.广藿香叶挥发油抗炎作用机制实验研究[J].中国实用医药,2015,10(2):249-251.
[17] 何兴丽,冉文婧,王永义,等.主客观听力检测相关性研究进展[J].中国工业医学杂志,2017(1):29-32.
[18] 赵兴泉,钟渠,陈茜.分泌性中耳炎研究现状[J].国际耳鼻咽喉头颈外科杂志,2017,41(3):136-140.
[19] 王梦航,李水静,韩琳,等.MUC5AC和TNF-α在大鼠急性分泌性中耳炎模型中的表达变化[J].中华耳科学杂志,2017,15(3):329-333.
[20] 迟作华,刘振,肖平.Tregs及IL-6、IL-10、TNF在急性分泌性中耳炎患者中的变化及意义[J].实用医学杂志,2016,32(2):255-257.
[21] 马慧琴,张治军.分泌性中耳炎相关细胞因子免疫机制研究进展[J].山东大学耳鼻喉眼学报,2012,26(4):78-81.
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