模拟微重力对大鼠脉搏血氧饱和度测量结果的影响研究
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摘要
本论文旨在通过大鼠尾部悬吊方式建立模拟微重力动物模型,研究微重力环境对无创方法测量大鼠血氧饱和度结果的影响。分别在饲养第14天、21天和28天使用脉搏血氧仪、血气分析仪测量实验组、对照组大鼠的脉搏血氧饱和度(SpO_2)及动脉血氧饱和度(SaO_2)。配对t检验结果显示,与对照组相比,实验组大鼠的SpO_2在第14天(P<0.05)、21天(P<0.05)和28天(P<0.05)均明显低于SaO_2值。方差分析结果显示,SpO_2值与造模时间有关,而SaO_2值与造模时间无关。上述结果表明,现有常规脉搏血氧仪可能不适用于空间微重力环境下的血氧饱和度测量。
To study the effect of microgravity on peripheral oxygen saturation(SpO_2) in rats, tail-suspended rats were applied to simulate microgravity environment. SpO_2 and arterial oxygen saturation(SaO_2) were measured by pulse oximeter and arterial blood gas analyzer(ABGA) respectively on the 14 th day, 21 st day and 28 th day in tail-suspended group and control group. Paired t-test shows that SpO_2 was significantly lower than SaO_2 in tail-suspended group on the14 th day(P < 0.05), the 21 st day(P < 0.05) and the 28 th day(P < 0.01). The ANOVA results shows that modeling time had significant effect on SpO_2 value but no effect on SaO_2 value in tail-suspended group. These results indicate that pulse oximeter may be not suitable for oxygen saturation test in microgravity environment.
To study the effect of microgravity on peripheral oxygen saturation(SpO_2) in rats, tail-suspended rats were applied to simulate microgravity environment. SpO_2 and arterial oxygen saturation(SaO_2) were measured by pulse oximeter and arterial blood gas analyzer(ABGA) respectively on the 14 th day, 21 st day and 28 th day in tail-suspended group and control group. Paired t-test shows that SpO_2 was significantly lower than SaO_2 in tail-suspended group on the14 th day(P < 0.05), the 21 st day(P < 0.05) and the 28 th day(P < 0.01). The ANOVA results shows that modeling time had significant effect on SpO_2 value but no effect on SaO_2 value in tail-suspended group. These results indicate that pulse oximeter may be not suitable for oxygen saturation test in microgravity environment.
引文
1于巍.反射式血氧饱和度无创检测的实验研究.广州:南方医科大学,2006.
2华子千,梁学,梁宇和,等.在空间微重力条件下斑头雁血红蛋白晶体生长.北京大学学报:自然科学版,1999,35(2):237-242.
3曹慧玲,马晓亮,郭云珠,等.空间微重力环境下蛋白质晶体生长的研究进展.材料导报,2011,25(15):69-74,95.
4梁宇和,查正,华子千,等.空间和地面条件下斑头雁血红蛋白晶体结构的比较研究.北京大学学报:自然科学版,2001,37(4):483-489.
5 陈万春,宋友庭.空间微重力环境中的晶体生长研究进展.科技导报,2012,30(2):46-57.
6Hanna G,Fontanella A,Palmer G,et al.Automated measurement of blood flow velocity and direction and hemoglobin oxygen saturation in the rat lung using intravital microscopy.Am J Physiol Lung Cell Mol Physiol,2013,304(2):L86-L91.
7Müller H C,Hellwig K,Rosseau S,et al.Simvastatin attenuates ventilator-induced lung injury in mice.Critical Care,2010,14(4):R143.
8Tipton C M.Animal models and their importance to human physiological responses in microgravity.Med Sci Sports Exerc,1996,28(10 Suppl):S94-100.
9Dong Qi,Shen Xianyun,Chen Jianhe,et al.Effects of simulated weightlessness on erythrocyte deformability in rats.Space Med Med Eng(Beijing),1997,10(4):240-244.
10董丽,王琼,刘新民,等.地面模拟失重实验方法概况.中国实验动物学报,2013,21(5):90-94.
2华子千,梁学,梁宇和,等.在空间微重力条件下斑头雁血红蛋白晶体生长.北京大学学报:自然科学版,1999,35(2):237-242.
3曹慧玲,马晓亮,郭云珠,等.空间微重力环境下蛋白质晶体生长的研究进展.材料导报,2011,25(15):69-74,95.
4梁宇和,查正,华子千,等.空间和地面条件下斑头雁血红蛋白晶体结构的比较研究.北京大学学报:自然科学版,2001,37(4):483-489.
5 陈万春,宋友庭.空间微重力环境中的晶体生长研究进展.科技导报,2012,30(2):46-57.
6Hanna G,Fontanella A,Palmer G,et al.Automated measurement of blood flow velocity and direction and hemoglobin oxygen saturation in the rat lung using intravital microscopy.Am J Physiol Lung Cell Mol Physiol,2013,304(2):L86-L91.
7Müller H C,Hellwig K,Rosseau S,et al.Simvastatin attenuates ventilator-induced lung injury in mice.Critical Care,2010,14(4):R143.
8Tipton C M.Animal models and their importance to human physiological responses in microgravity.Med Sci Sports Exerc,1996,28(10 Suppl):S94-100.
9Dong Qi,Shen Xianyun,Chen Jianhe,et al.Effects of simulated weightlessness on erythrocyte deformability in rats.Space Med Med Eng(Beijing),1997,10(4):240-244.
10董丽,王琼,刘新民,等.地面模拟失重实验方法概况.中国实验动物学报,2013,21(5):90-94.