脑红蛋白在双峰驼视网膜中的表达研究
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摘要
为了探讨双峰驼视网膜全层中脑红蛋白(NGB)的分布特征,选择健康成年双峰驼,利用免疫组化SP法染色,观察NGB在双峰驼视网膜各层中的表达情况。结果显示,NGB在除视网膜外核层之外的其他区域均有表达,且存在表达强度的差异,其主要分布在节细胞及双极细胞的细胞质、光感受器细胞及色素上皮细胞中。以上结果表明,NGB几乎分布于视网膜全层,提示在极端生存环境适应性中,NGB对维持视网膜中的氧稳态及参与光损伤修复机制过程中可能具有非常重要的生理意义。NGB在双峰驼光感受器外节段中的阳性表达,丰富了NGB在哺乳动物视网膜中分布的形态学资料,并且对其功能研究提供了有价值的线索。
For the purpose of studing the distribution of neuroglobin proteins(NGB) in retina of adult Bactrian camel,immunohistochemical SP method was used to observe the distribution of NGB in the retina of healthy adult Bactrian camels.The results showed that strongly NGB proteins were seen in all of retina except outer nuclear layer.The mainly retinal cell's types were retinal ganglion cells,bipolar cells,photoreceptor cells and retinal pigment epithelium cells.In retinal ganglion cells and bipolar cells,NGB was located in the cytoplasm,not expressed in the nucleus.These observations suggested that NGB was almost expressed in all layers of the retina and might play an important role in oxygen homeostasis of retina,mechanism process of light damage repair and adapting extreme environments.Bactrian camel photoreceptor outer segments find that positive expression for NGB in the mammalian retina distribution provided valuable morphological data and valuable clues for its functional research.
For the purpose of studing the distribution of neuroglobin proteins(NGB) in retina of adult Bactrian camel,immunohistochemical SP method was used to observe the distribution of NGB in the retina of healthy adult Bactrian camels.The results showed that strongly NGB proteins were seen in all of retina except outer nuclear layer.The mainly retinal cell's types were retinal ganglion cells,bipolar cells,photoreceptor cells and retinal pigment epithelium cells.In retinal ganglion cells and bipolar cells,NGB was located in the cytoplasm,not expressed in the nucleus.These observations suggested that NGB was almost expressed in all layers of the retina and might play an important role in oxygen homeostasis of retina,mechanism process of light damage repair and adapting extreme environments.Bactrian camel photoreceptor outer segments find that positive expression for NGB in the mammalian retina distribution provided valuable morphological data and valuable clues for its functional research.
引文
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[22]ROBERTS P A,GAFFNEY E A,LUTHERT P J,et al.Retinal oxygen distribution and the role of neuroglobin[J].Journal of Mathematical Biology,2016,73(1):1-38.
[2]GEUENS E,BROUNS I,FLAMEZ D,et al.A globin in the nucleus[J].Journal of Biological Chemistry,2003,278(33):30417-30420.
[3]SCHMIDT M,GIESSL A,LAUFS T,et al.How does the eye breathe?Evidence for neuroglobin-mediated oxygen supply in the mammalian retina[J].Journal of Biological Chemistry,2003,278(3):1932-1935.
[4]高雪晋.脑红蛋白在西门塔尔牛主要器官组织中分布的研究[D].兰州:甘肃农业大学,2015.GAO Xue-jin.Research of distribution of neuroglobin in major organs and tissues of the simmental[D].Lanzhou:Gansu Agricultural University,2015.(in Chinese)
[5]WEI X,DENG Y,LIU X,et al.How can photoreceptor cells breathe in the retina?Neuroglobin may be the answer[J].Neural Regeneration Research,2010,5(9):720-720.
[6]ANDERSON B,J R.Ocular effects of changes in oxygen and carbon dioxide tension[J].Transactions of the American Opbtbalmol Society,1968,66(1):423-474.
[7]GREENBERG D A,JIN K,KHAN A A.Neuroglobin:an endogenous neuroprotectant[J].Current Opinion in Pharmacology,2008,8(1):20-24.
[8]冉建华,孙善全,毕恣,等.脑红蛋白m RNA在大鼠眼球中的表达[J].重庆医学,2005,34(7):1035-1036.RAN Jian-hua,SUN Shan-quan,BI Zi,et al.The expression of NGB m RNA in adult rat eye[J].Chongqing Medicine,2005,34(7):1035-1036.(in Chinese)
[9]李耀宇,刘惠玲,童绎,等.脑红蛋白在大鼠眼球中分布的免疫组织化学研究[J].眼科研究,2006,24(5):461-464.LI Yao-yu,LIU Hui-ling,TONG Yi,et al.Normal distribution of neuroglobin in proteins in eyeball of adult rats[J].Chinese Ophthalmic Research,2006,24(5):461-464.(in Chinese)
[10]OSTOJI J,GROZDANI S D,SYED N A,et al.Patterns of distribution of oxygen-binding globins,neuroglobin and cytoglobin in human retina[J].Archives of Ophthalmology,2008,126(11):1530-1536.
[11]OSTOJI J,SAKAGUCHI D S,DE LATHOUDER Y,et al.Neuroglobin and cytoglobin:oxygen-binding proteins in retinal neurons[J].Investigative Ophthalmology&Visual Science,2006,47(3):1016-1023.
[12]杨阳,刘霞,曾国敏,等.脑红蛋白在家兔视网膜中的分布[J].中国组织化学与细胞化学杂志,2015,24(2):139-144.YANG Yang,LIU Xia,ZENG Guo-min,et al.Distribution of neuroglobin in retina of adult rabbits[J].Chinese Journal of Histochemistry and Cytochemistry,2015,24(2):139-144.(in Chinese)
[13]杨阳,刘霞,高雪晋,等.脑红蛋白在绵羊视网膜中的分布[J].解剖学杂志,2015,(3):275-278.YANG Yang,LIU Xia,GAO Xue-jin,et al.Distribution of neuroglobin in the retina of adult sheep[J].Chinese Journal of Anatomy,2015,(3):275-278.(in Chinese)
[14]郭延俭,王绿林,王朝阳,等.牛眼球石蜡切片制作方法的探索[J].中国奶牛,2013,17(1):11-13.GUO Yan-jian,WANG L譈-lin,WANG Zhao-yang,et al.Exploration for a paraffin production method of cow eyeballs[J].China Dairy Cattle,2013,17(1):11-13.(in Chinese)
[15]BENTMANN A,SCHMIDT M,REUSS S,et al.Divergent distribution in vascular and avascular mammalian retinae links neuroglobin to cellular respiration[J].Journal of Biological Chemistry,2005,280(21):20660-20665.
[16]徐琼,黄晓峰,魏小燕,等.成年大鼠视网膜中脑红蛋白表达的亚细胞分布[J].国际眼科杂志,2009,9(1):35-37.XU Qiong,HUANG Xiao-feng,WEI Xiao-yan,et al.Ultrastructural study of intraretinal distribution of neuroglobin proteins in normal adult rats[J].International Journal of Ophthalmology,2009,9(1):35-37.(in Chinese)
[17]COSTA B L,FAWCETT R,LI G Y,et al.Orally dminisatered epigallocatechin gallate attenuateslightinduced photoreceptor damage[J].Brain Research Bulletin,2008,76(4):412-423.
[18]FORDEL E,THIJS L,MARTINET W,et al.Anoxia or oxygen and glucose deprivation in SH-SY5Y cells:a step closer to the unraveling of neuroglobin and cytoglobin functions[J].Gene,2007,398(2):114-122.
[19]NAYAK G,PRENTICE H M,MILTON S L.Role of neuroglobin in regulating reactive oxygen species in the brain of the anoxia-tolerant turtle Trachemys scripta[J].Journal of Neurochemistry,2010,110(2):603-612.
[20]CRINGLE S J,YU D Y,PAULA K V,et al.Intraretinal oxygen consumption in the rat in vivo[J].Investigative Ophthalmology&Visual Science,2002,43(6):1922-1927.
[21]李秋明,郑广瑛.眼科应用解剖学[M].郑州:郑州大学出版社,2013:121.LI Qiu-ming,ZHENG Guang-ying.Ophthalmic appli ed anatomy[M].Zhengzhou:Zhengzhou University Press,2013:121.(in Chinese)
[22]ROBERTS P A,GAFFNEY E A,LUTHERT P J,et al.Retinal oxygen distribution and the role of neuroglobin[J].Journal of Mathematical Biology,2016,73(1):1-38.
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