脑红蛋白在糖尿病大鼠视网膜病变发展中的表达变化

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英文篇名：Expression changes of neuroglobin in the development of diabetic rat retinopathy 作者：刘琼 ; 于健 ; 陈林江 英文作者：LIU Qiong;YU Jian;CHEN Lin-jiang;Department of Ophthalmology, Nanfang Hospital of Southern Medical University; 关键词：脑红蛋白 ; 视网膜 ; 糖尿病 英文关键词：Neuroglobin;;Retina;;Diabetes 中文刊名：ZSSA 英文刊名：China Practical Medicine 机构：南方医科大学南方医院眼科; 出版日期：2019-05-10 出版单位：中国实用医药 年：2019 期：v.14 基金：广州市科技计划项目(项目编号:2013J4100105);; 南方医院院长基金(项目编号:2012A002) 语种：中文; 页：ZSSA201913102 页数：3 CN：13 ISSN：11-5547/R 分类号：196-198

摘要

目的探讨脑红蛋白(NGB)在糖尿病大鼠视网膜病变发展中的表达变化。方法 30只雄性SD大鼠,随机分为糖尿病组(DR组)和正常对照组(N组),每组15只。DR组大鼠腹腔注射链脲佐菌素STZ构建糖尿病大鼠动物模型。采用原位杂交法、免疫组化、蛋白质印迹法(Western blotting)检测NGB表达变化和分布特点。结果诱导12周后, DR组大鼠体重为(309.0±11.0)kg,明显轻于N组的(584.1±25.4)kg,血糖为(30.1±5.4)mmol/L,明显高于N组的(7.5±5.3)mmol/L,差异均有统计学意义(P<0.05)。DR组视网膜组织NGB表达增加,明显高于N组,差异有统计学意义(P<0.01)。原位杂交示DR组神经节细胞层、内核层、外核层、光感受器层可见强阳性表达。结论在糖尿病大鼠视网膜病变发展过程中, NGB可能参与视网膜的神经保护。
        Objective To discuss the expression changes of neuroglobin(NGB) in the development of diabetic rat retinopathy. Methods A total of 30 male SD rats were randomly divided into diabetic group(DR group) and normal control group(N group), with 15 rats in each group. In DR group, streptozotocin STZ was injected intraperitoneally to establish diabetic rat model. The expression and distribution characteristics of NGB were detected by in situ hybridization, immunohistochemistry and Western blotting. Results After 12 weeks of induction, DR group had obviously lighter rat weight as(309.0±11.0) kg than(584.1±25.4) kg in N group, and obviously higher blood glucose as(30.1±5.4) mmol/L than(7.5±5.3) mmol/L in N group. Their difference was statistically significant(P<0.05). DR group had expression of NGB in retina of increased by 55.68% compared with that of N group, and the difference was statistically significant(P<0.01). Situ hybridization showed strong positive expression in ganglion cell layer, inner nuclear layer, outer nuclear layer and photoreceptor layer of DR group. Conclusion NGB may be involved in retinal neuroprotection during the development of diabetic rat retinopathy.

引文

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