小麦野生近缘植物多药抗性基因PDR7的生物信息学分析
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摘要
小麦的野生近缘植物,尤其山羊草属中含有丰富的抗病虫基因,是小麦遗传育种的重要基因来源。针对禾谷镰刀菌入侵引起的小麦赤霉病,在山羊草属中尚未找到相应抗性基因。多药抗性基因(pleiotropic drug resistance, PDR)是ABC (ATP-binding cassette)转运蛋白基因三大亚家族中的一个,具有对生物和非生物胁迫的广谱抗性。本研究以D、C、U、M、S染色体基组的山羊草属植物为研究材料,对PDR7基因进行分离鉴定和生物信息学分析。结果表明,这些PDR7基因为半个PDR基因,具有PDR基因的典型结构域,是膜蛋白;同源序列对比和进化分析均表明,在山羊草属植物中,PDR7基因具有高度的保守性。以AePDR7为代表进行了启动子预测,表明AePDR7对禾谷镰刀菌等真菌、脱氧雪腐镰刀烯醇(deoxynivalenol, DON)、生物激素、盐胁迫、冷胁迫、干旱胁迫等均有响应元件,说明其可能是一个广谱抗性基因。
Wild relatives of wheat, especially Aegilops plants that are rich in insect-resistance genes, is an important source for wheat heredity and breeding. Yet till now, no corresponding resistance genes of wheat scab which caused by fusarium head blight has not been found. Pleiotropic drug resistance(PDR) gene is one of the three subfamiliesof ATP-binding cassette(ABC) transporter protein gene. It has broad spectrum resistance to biotic and abiotic stress. In this research, we used D, C, U, M and S chromosome basis set of Aegilops plants as materials to isolate and identify the PDR7 genes, and analyzed with bioinformatics method. The results showed that these PDR7 genes were half-size PDR gene, and they all had the typical structure region of PDR genes, which was membrane proteins. Homologous alignment and molecular evolution analysis showed that PDR7 was quite conserved in Aegilops plants. Use AePDR7 as the representative, promoter prediction showed that AePDR7 had response elements of fungi such as Fusarium graminearum, deoxynivalenol(DON), biotic stress, salt stress, cold stress, drought stress and so on which indicated that it might be a broad-spectrum disease resistance gene.
Wild relatives of wheat, especially Aegilops plants that are rich in insect-resistance genes, is an important source for wheat heredity and breeding. Yet till now, no corresponding resistance genes of wheat scab which caused by fusarium head blight has not been found. Pleiotropic drug resistance(PDR) gene is one of the three subfamiliesof ATP-binding cassette(ABC) transporter protein gene. It has broad spectrum resistance to biotic and abiotic stress. In this research, we used D, C, U, M and S chromosome basis set of Aegilops plants as materials to isolate and identify the PDR7 genes, and analyzed with bioinformatics method. The results showed that these PDR7 genes were half-size PDR gene, and they all had the typical structure region of PDR genes, which was membrane proteins. Homologous alignment and molecular evolution analysis showed that PDR7 was quite conserved in Aegilops plants. Use AePDR7 as the representative, promoter prediction showed that AePDR7 had response elements of fungi such as Fusarium graminearum, deoxynivalenol(DON), biotic stress, salt stress, cold stress, drought stress and so on which indicated that it might be a broad-spectrum disease resistance gene.
引文
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Chhuneja P.,Kaur S.,Goel R.K.,Aghaee-Sarbarzeh M.,Prashar M.,and Dhaliwal H.S.,2008b,Transfer of leaf rust and stripe rust resistance from Aegilops umbellulata Zhuk.to bread wheat(Triticum aestivum L.),Genet.Resour.Crop Evol.,55(6):849-859
Donini P.,Koebner R.M.,and Ceoloni C.,1995,Cytogenetic and molecular mapping of the wheat-Aegilops longissimi chromatin breakpoints in powdery mildew-resistant introgression lines,Theor.Appl.Genet.,91(5):738-743
Eichhorn H.,Klinghammer M.,Becht P.,and Tenhaken R.,2006,Isolation of a novel ABC-transporter gene from soybean induced by salicylic acid,J.Exp.Bot.,57(10):2193-2201
Gupta S.K.,Charpe A.,Koul S.,Prabhu K.V.,and Haq Q.M.,2005,Development and validation of molecular markers linked to an Aegilops umbellulata-derived leaf-rust-resistance gene,Lr9,for marker-assisted selection in bread wheat,Genome,48(5):823-830
Higgins C.F.,2001,ABC transporters:physiology,structure and mechanism-an overview,Res.Microbiol.,152(3-4):205-210
Hou W.Q.,2014,Identification and BSMV-VIGS analysis of candidate genes resistant to fusarium head blight,Dissertation for Ph.D.,Shangdong Agricultural University,Supervisor:Kong L.R.,pp.56-77(侯文倩,2014,小麦赤霉病抗性相关基因的分离鉴定及BSMV-VIGS功能验证,博士学位论文,山东农业大学,导师:孔令让,pp.56-77)
Hu Y.Y.,Feng L.N.,Ji H.L.,Sun Y.,Zhang N.,Wei X.J.,Yang W.X.,Liu D.Q.,and Jia J.Z.,2011,SSR mapping of leaf rust resistance gene LrY192 in Aegilops tauschii Y192,Zhongguo Nongye Kexue(Scientia Agriculture Sinica),44(10):2022-2028(胡亚亚,冯丽娜,冀红柳,孙一,张娜,魏学军,杨文香,刘大群,贾继增,2011,粗山羊草Y192中抗叶锈基因LrY192的SSR定位,中国农业科学,44(10):2022-2028)
Huang L.,Brooks S.A.,Li W.,Fellers J.P.,Trick H.N.,and Gill B.S.,2003,Map-based cloning of leaf rust resistance gene Lr21 from the large and polyploid genome of bread wheat,Genetics,164(2):655-664
Karpowich N.,Martsinkevich O.,Millen L.,Yuan Y.R.,Dai P.L.,MacVey K.,Thomas P.J.,and Hunt J.F.,2001,Crystal structure of the MJ1267 ATP binding cassette reveal and induced-fit effect at the ATPase active site of an ABC transporter,Structure,9(7):571-586
Kobae Y.,Sekino T.,Yoshioka H.,Nakagawa T.,Martinoia E.,and Maeshima M.,2006,Loss of AtPDR8,a plasma membrane ABC transporter of Arabidopsis thaliana,causes hypersensitive cell death upon pathogen infection,Plant Cell Physiol.,47(3):309-318
Lang J.,Zhang H.Q.,and Yang H.,2014,Research advances in application of Aegilops tauschii on wheat genetics and breeding,Zhongguo Nongye Daxue Xuebao(Journal of China A-gricultural University),19(5):269-274(朗杰,张海泉,杨虹,2014,粗山羊草在小麦遗传育种中的应用研究进展,中国农业大学学报,19(5):269-274)
Lee M.,Lee K.,Lee J.,Noh E.W.,and Lee Y.,2005,AtPDR12contributes to lead resistance in Arabidopsis,Plant Physiol.,138(2):827-836
Li M.J.,Li Y.Q.,Zhang S.C.,Zhang N.,and Shi Z.L.,2017,Isolation and functional analysis of coding and promoter regions of LEAsm gene in wheat,Mailei Zuowu Xuebao(Journal of Triticeae Crops),37(2):145-152(李孟军,李亚青,张士昌张楠,史占良,2017,小麦LEAsm基因及其启动子的克隆与功能研究,麦类作物学报,37(2):145-152)
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