膀胱脱细胞基质预凝胶水溶液的微流变特性研究

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英文篇名：Microrheological properties of bladder acellular matrix hydrogel during gelation 作者：刘文景 ; 范苏娜 ; 张慧慧 ; 邵惠丽 ; 宋鲁杰 ; 黄建文 ; 张耀鹏 英文作者：Liu Wenjing;Fan Suna;Zhang Huihui;Shao Huili;Song Lujie;Huang Jianwen;Zhang Yaopeng;State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University;Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital; 关键词：膀胱脱细胞基质 ; 水凝胶 ; 微流变性能 ; 溶胶-凝胶转变 英文关键词：bladder acellular matrix;;hydrogel;;microrheological properties;;sol-gel transition 中文刊名：HCSY 英文刊名：Synthetic Technology & Application 机构：纤维材料改性国家重点实验室东华大学材料科学与工程学院;上海交通大学附属第六人民医院泌尿外科; 出版日期：2019-03-28 出版单位：合成技术及应用 年：2019 期：v.34;No.125 基金：国家重点研发计划,2018YFC1105802;; 国家自然科学基金,21674018,81600524 语种：中文; 页：HCSY201901006 页数：5 CN：01 ISSN：32-1414/TQ 分类号：14-18

摘要

本研究采用光学微流变仪对不同浓度膀胱脱细胞基质(BAM)预凝胶水溶液的动态成胶过程进行考察。结果表明,在BAM水凝胶成胶过程中,颗粒均方位移(MSD)曲线均出现平台区,且流动因子(FI)逐渐减小,即发生溶胶-凝胶转变;随着BAM浓度的增加,MSD曲线平台区高度降低且向右移动,宏观黏度因子(MVI)和弹性因子(EI)逐渐增大,网格尺寸(ξ)减小,即凝胶粘弹性增强。本研究为组织工程应用中原位注射BAM水凝胶提供了流变学参考。
        In this study, the microrheological properties of bladder acellular matrix(BAM) pre-gel solutions with different mass concentrations were investigated during gelation by optical microrheometer. Results showed that the mean square displacement(MSD) of all BAM pre-gel solution generated a plateau region and the fluidity index(FI) gradually decreased. Furthermore, as the BAM concentration increased, the platform height of MSD curves decreased, and the curves shifted to right. The macroscopic viscosity index(MVI), elasticity index(EI) gradually increased, while the mesh size decreased, which indicated that viscoelasticity of BAM hydrogel increased. This study provided a rheological reference for the in situ injection of BAM hydrogels in tissue engineering.

引文

[1] Aalaie J, Vasheghani-Farahani E, Rahmatpour A, et al. Gelation rheology and water absorption behavior of semi-interpenetrating polymer networks of polyacrylamide and carboxymethyl cellulose [J]. Journal of Macromolecular Science, Part B, 2013, 52(4): 604-613.
    [2] Lucio B, de la Fuente J L. Rheokinetic analysis on the formation of metallo-polyurethanes based on hydroxyl-terminated polybutadiene [J]. European Polymer Journal, 2014, 50: 117-126.
    [3] Chen Y Q, Chou P L, Cheng C Y, et al. Microrheology of human synovial fluid of arthritis patients studied by diffusing wave spectroscopy [J]. Journal of Biophotonics, 2012, 5(10): 777-784.
    [4] Larsen T H, Furst E M. Microrheology of the liquid-solid transition during gelation [J]. Physical Review Letters, 2008, 100(14): 146001.
    [5] Tisserand C, Fleury M, Brunel L, et al. Analytical series soft matter analysis by means of microrheology [J]. Coatingstech, 2012, 9(3): 46-51.
    [6] Bellour M, Skouri M, Munch J P, et al. Brownian motion of particles embedded in a solution of giant micelles [J]. The European Physical Journal E-Soft Matter, 2002, 8(4): 431-436.
    [7] Feng C, Xu Y M, Fu Q, et al. Evaluation of the biocompatibility and mechanical properties of naturally derived and synthetic scaffolds for urethral reconstruction [J]. Journal of Biomedical Materials Research Part A, 2010, 94a(1): 317-325.
    [8] Freytes D O, Martin J, Velankar S S, et al. Preparation and rheological characterization of a gel form of the porcine urinary bladder matrix [J]. Biomaterials, 2008, 29(11): 1630-1637.
    [9] Tisserand C, Fleury M, Brunel L, et al. Passive microrheology for measurement of the concentrated dispersions stability [J]. Progress in Colloid & Polymer Science, 2012, 139: 101-105.
    [10] Chen Y Q, Cheng C Y, Chiang C C, et al. Microrheology of sodium polystyrene sulfonate (NaPSS) solutions with different polymer concentrations and molecular weights studied by diffusing wave spectroscopy [C]. Holography, Diffractive Optics, and Applications IV. International Society for Optics and Photonics, 2010, 7848: 78480H.
    [11] Min W, Yang X Q. Effects of molecular weight of dextran on the rheological behavior and texture properties of soy protein isolate cold-set gels [J]. Science & Technology of Food Industry, 2010, 31(5): 72-71.
    [12] Brightman A O, Rajwa B P, Sturgis J E, et al. Time-lapse confocal reflection microscopy of collagen fibrillogenesis and extracellular matrix assembly in vitro [J]. Biopolymers, 2000, 54(3): 222-234.
    [13] Oelschlaeger C, Schopferer M, Scheffold F, et al. Linear-to-branched micelles transition: A rheometry and diffusing wave spectroscopy (DWS) study [J]. Langmuir, 2009, 25(2): 716-723.